TALANTA
·
2019
·
IF 4.916
Tian-Tian Wang,Chon kit Lio,Hui Huang,Run-Yue Wang,Hua Zhou,Pei Luo,Lin-Sen Qing
Urinary glucose determination using a glucose test strip is simple and convenient in daily self-monitoring of diabetes. However, diabetic patients exhibit acquired impaired color vision (ICV), which results in the inability to discriminate between hues. Even with the assistance of a color chart, it is still not easy for these patients to read the urinary glucose results with the naked eye. In this study, a smartphone camera using an image-based colorimetric detection method was successfully developed for quantitative analysis of urine glucose. A horseradish peroxidase-hydrogen peroxide-3,3′5,5′-tetramethylbenzidine (HRP–H 2 O 2 –TMB) system was optimized for a reliable and gradual color fading process via a glucose oxidase (GOD) catalyzed oxidation reaction. The color changes of the peroxidase-H 2 O 2 enzymatic reactions in the 96-well microplate were captured by a smartphone RGB camera with subsequent detection of red, green, and blue (RGB) intensities decreasing at each image pixel. The highly quantitative relationships between the glucose concentrations and the color characteristic values of the blue channel of the captured images were successfully established. The high accuracy of this method was demonstrated in urine glucose measurements with a linear response over the 0.039 mg mL −1 to 10.000 mg mL −1 glucose concentration range and a 0.009 mg mL −1 detection limit. The method has great potential as a point-of-need platform for diabetic patients with defective color vision and features high accuracy and low cost.
研究领域:
医学诊断
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生物化学
·
移动健康技术
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DOI
高影响因子
Journal of Materials Chemistry C
·
2018
·Vol. 7
·期 4
·1065-1071
·
IF 5.976
Zhen Qin,Qiutong Wang,Chengzhong Wang,Dongfeng Xu,Guiping Ma,Kai Pan
White-light-emitting materials are an important component for realizing full-color OLED devices. However, it is still a challenge to achieve white-light emission from one single fluorescent dye, as the essential requirement of the emission spectrum is that it should be sufficiently broad to cover the entire visible-light region. Controlling the energy transfer between two or three fluorescent dyes is a feasible approach for achieving white-light emission, and this has attracted widespread attention from many researchers. In this study, a white-light-emitting Janus nanofiber film was fabricated using a side-by-side electrospinning method. RGB fluorescent molecules were used to form a donor–acceptor system, where the red and blue fluorescent molecules were located on the PAN-side of the nanofiber, and the green fluorescent molecules were located on the PVP-side of the nanofiber. The Janus structure enabled an efficient spatial separation of the RGB fluorescent molecules within the nanofiber while rationally regulating the two-step energy transfer effect between the RGB fluorescent molecules, resulting in the emission of uniform white light from every individual microscopic-sized nanofiber. Such nanofiber films have great potential for use in next-generation flexible display applications due to their excellent performance and micro scale.
研究领域:
材料科学
·
光电技术
·
纳米技术
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Xuedong Zhang,Zhuoheng Wu,Jian Chen,Dongliang Yang,Zhenlei Zhong,Yinghao Li,Yinfu Lu,Zemin He,Yuzhen Zhao,Yi Luan
To address technical bottlenecks associated with complex fabrication processes and extended manufacturing duration of conventional three-layer cholesteric liquid crystal (CLC) structures, photolithography and a chiral molecular precision diffusion strategy were integrated to prepare monolayer color-reflective photonic display panels. Moreover, by developing monolayer-encapsulated CLC photonic displays, we effectively tackled key technical problems encompassing fine microstructure design, isolated RGB pixel fabrication, and high-precision color tuning of CLC. It is notable that the electric-field-driven CLC switching process was realized through red, green, and blue pixel unit color superposition. The fabricated monolayer color display possesses electric-field-regulated pattern switching functionality. Furthermore, a multimode anticounterfeiting model was successfully constructed based on the Bragg selective reflection characteristics of CLC. Within the model, the original matrix pattern presents varied visual information with obvious differences in color and brightness under varying illumination. Combining specific light source illumination with the matrix encoding table facilitates efficient decoding of encrypted information, significantly boosting CLC anticounterfeiting performance.
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DOI
PubMed
高影响因子
MATERIALS RESEARCH BULLETIN
·
2020
·
IF 4.019
Enming Zhao,Xiaodan Liu,Dongyang Tang,Lu Liu,Guangyu Liu,Bao Zhou,Chuanxi Xing
To date, the white light lanthanide upconvertion consisting of RGB components usually originated from more than one type of emitter. Alternatively, Pr 3+ is a fascinating lanthanide ion featuring multiple visible emission bands, including RGB components that in principle enable Pr 3+ to emit white light. In this paper, we report, for the first time to our knowledge, white light upconversion through dual sensitization routes in Nd/Yb/Pr triply doped NaYF 4 microrods upon 800 nm excitation. It is found that the dual sensitization is more efficient than single sensitization for Pr 3+ upconversion. In addition, the temperature sensing behaviors of the proposed triply doped microrods are comparatively investigated using different emission bands. This work presents a novel white light emitting material upon 800 nm excitation, and further demonstrates the feasibility of multichannel thermometry using the proposed material.
研究领域:
材料科学
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光学
·
纳米技术
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产品关联
DOI
高影响因子
Analytical Methods
·
2026
·
IF 3
Shengyi Fang,Sheng Ye,Dan Ouyang,Songyan Tie,Jianzhong Cao
Nanocatalysts based on metal-organic frameworks exhibit limited catalytic activity due to their symmetrical inactive structures, thereby restricting their application in nanozyme-linked immunoassays (NLISA). This work constructed geometrically structure expanded Cu-Co bimetallic metal-organic frameworks (GS-CCBM) nanozymes through a two-step hydrothermal and alkali treatment, enabling a specific nanozyme-based immunoassay for the target alpha-fetoprotein (AFP). The constructed GS-CCBM nanozyme demonstrated excellent substrate affinity (Km = 1.79 mM) and H2O2 reaction rate (Vm = 8.569 μmol min-1). Based on this, a signal probe was constructed that enables changes in the absorbance and color of the catalytic system in the presence of the target AFP. Semi-quantitative and quantitative analyses were performed using a self-developed color value extraction system. Under optimized conditions, the constructed NLISA method demonstrated an acceptable working range (0.01–50 ng mL⁻¹) and a sensitive detection limit (LOD = 4.7 pg mL⁻¹) for AFP. This approach innovatively proposes a novel strategy for constructing highly efficient MOF-based nanozymes with catalytic activity, offering a new method for developing novel clinical detection techniques.
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DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2019
·
IF 6.97
Xin Li,Siqun Li,Qingyun Liu,Zhiqiang Cui,Zhengbo Chen
The detection and discrimination of biothiols is of cardinal significance because of their key roles in biological systems and numerous diseases. The present work has developed a simple but effective colorimetric sensor array for identification of biothiols. The sensor array is based on utilization of triple-channel properties (the red (R), green (G), and blue (B) alterations) of polydopamine (PDA)-functionalized gold nanoparticles (AuNPs). Five metal ions (Fe3+, Mg2+, Ca2+, Ag+, and Hg2+) as sensing elements are anchored on the surface of PDA–AuNPs through chelating reaction between metal ions and PDA. Different thiols show distinct binding affinities toward these metal ions, generating diverse colorimetric response patterns (i.e., RGB variations) as “fingerprints” related to each specific biothiol, which can be quantitatively differentiated by linear discriminant analysis (LDA) and hierarchical clustering analysis (HCA). The sensor array can well discriminate six biothiols (dithiothreitol, l-cysteine, glutathione, mercaptoethanol, mercaptosuccinic acid, and mercaptoacetic acid) with 100% accuracy and high sensitivity at the 30 nM level. The sensing strategy, with simple preparation, fast response, excellent sensitivity, and especially stable and high-throughput signal output, is promising for practical applications in artificial olfactory systems.
研究领域:
分析化学
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生物医学工程
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纳米技术
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产品关联
DOI
高影响因子
Laser & Photonics Reviews
·
2022
·Vol. 17
·期 2
·2200531
·
IF 10.947
Shuai Zhou,Yang Wang,Po Hu,Wei Zhong,Hong Jia,Jianrong Qiu,Jiajun Fu
Orthogonal upconversion nanoparticles (UCNPs) have received increasing research attention due to their unique optical performance. However, the establishment of the orthogonal UCNPs emitting primary red/green/blue (RGB) UCL remains a great challenge. Herein, RGB-switchable NaErF 4 -cored core–multishell UCNPs (RGB-UCNPs) are constructed through the coordinative implementation of outside-in cascaded photon harvesting and interfacial energy-transfer (IET) inhibition. By modulating several constructional factors, the power-density-independent red, green, and blue luminescence in high color purity is readily available in the elaborated RGB-UCNPs under 1550, 808, and 980 nm excitations, respectively. Notably, full-color emissions, involving red, yellow, green, cyan, blue, magenta and white, are dynamically implemented in this single architecture through manipulating the excitation power of the combined 1550/808/980 nm lasers. These emission profiles of RGB-UCNPs make them promising in broad photonic applications. As a proof of concept, the viability of the logicalized information encryption strategy and smartphone APP-assisted multimodal luminescent QR code anti-counterfeiting technique is demonstrated, which are both established based on directly useable RGB-UCNPs with assistance of a self-built combined laser system. This work not only opens up a new avenue to gain RGB-switchable UCL and flexible full-color output in a single nanostructure, but also provides enlightenment for developing the application scenarios of multicolor-tunable UCNPs.
研究领域:
材料科学
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光学
·
纳米技术
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产品关联
DOI
高影响因子
Biosensors-Basel
·
2023
·Vol. 13
·期 7
·753
·
IF 5.4
Chonghui Yang,Yujing Yang,Gaozhen Zhao,Huan Wang,Yang Dai,Xiaowen Huang
The sensitive and rapid detection of microsamples is crucial for early diagnosis of diseases. The short response times and low sample volume requirements of microfluidic chips have shown great potential in early diagnosis, but there are still shortcomings such as complex preparation processes and high costs. We developed a low-cost smartphone-based fluorescence detection device (Smartphone-BFDD) without precision equipment for rapid identification and quantification of biomarkers on glass capillary. The device combines microfluidic technology with RGB image analysis, effectively reducing the sample volume to 20 μL and detection time to only 30 min. For the sensitivity of the device, we constructed a standard sandwich immunoassay (antibody–antigen–antibody) in a glass capillary using the N-protein of SARS-CoV-2 as a biological model, realizing a low limit of detection (LOD, 40 ng mL−1). This device provides potential applications for different biomarkers and offers wide use for rapid biochemical analysis in biomedical research.Keywords:smartphone;fluorescence;glass capillary;biomarkers;RGB
研究领域:
生物医学工程
·
分析化学
·
微流控
·
即时诊断
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Zhou Hongtao,Sun He,Zhang Zebin,Fang Xin,Bu Shengjun,Li Chenxi,Wan Yue,Li Nan,Hao Zhuo,Ma Chengyou,Lv Na,Wan Jiayu
This study presents a signal-amplified detection platform integrating magnetic separation, PtAuRu@PCN-224 nano-enzyme catalysis magnification, and smartphone RGB analysis. The developed detection system has the following advantages: ultra-high sensitivity, the detection limit in ultraviolet-visible light mode is 1 CFU/mL; it features rapid response and excellent selectivity, capable of distinguishing common foodborne pathogenic bacteria; the nanozyme exhibits excellent stability, with its activity remaining above 92% after being stored at 4℃ for 10 days; the recovery rates in four food samples were 98 to 103%; and the relative standard deviations were less than 3%. This platform combines the high catalytic performance of MOFs nano-enzymes with the convenience of RGB analysis on smartphones, providing a new solution for the detection of foodborne pathogens. Graphical abstract
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DOI
PubMed
高影响因子
Food Chemistry-X
·
2023
·
IF 6.1
Chenxi Jiang,Shuang Ye,Jing Xiao,Chao Tan,Huimin Yu,Xiaoli Xiong,Ke Huang,Yujia Deng,Zhirong Zou
A miniaturized/portable dual-mode colorimetric analytical system was established for inorganic arsenic determination in honey and drinking water samples. Hydride generation (HG) was utilized as a sampling technique for this colorimetric system, because of its high generation efficiency and efficient matrix separation. AsH 3 was generated via HG and then reacted with HAuCl 4 , gold nanoparticles (Au NPs) were formed on the paper sheet, leading the paper color changed from light yellow to dark blue, it could be readout by naked-eye (visual colorimetric mode) and a smartphone (RGB readout mode) simultaneously. The accuracy and potential application for field analysis were further confirmed by the analysis of two water samples, four honey samples and two certified reference water samples (BWB2440-2016 and GBW08650), good recoveries (90–116%) were obtained for those samples and their spiked samples.
研究领域:
分析化学
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环境科学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 8
·1-10
·
IF 6.408
Zhao Tingting,Yan Weizhen,Dong Fengqi,Hu Xinlong,Xu Yanli,Wang Zhenyu,Shen Yating,Wang Wanrong,Zhao Ye,Wei Wenmei
COVID-19 necessitates the development of reliable and convenient diagnostic tools. In this work, a facile 3D-printed smartphone platform was constructed that achieved reliable visual detection of SARS-CoV-2 by eliminating the effect of ambient light and fixing the camera position relative to the sample. The oligonucleotide probe is modified with orange-red-emitting TAMRA working as an internal standard and green-emitting FAM serving as a sensitive sensing agent. Under 365-nm UV excitation, the emission wavelengths of TAMRA and FAM are 580 nm and 518 nm, respectively. When the probes interact with the targets, the green fluorescence gradually restores while the orange-red fluorescence remains stable. Thus, a striking color transition from orange-red to green could be observed by the naked eye. The detection limit of SARS-CoV-2 nucleic acid is 0.23 nM, and the entire process of color change could be completed in 25 min. Furthermore, the RGB value analysis of the sample solution was conducted using a smartphone for reliable and reproducible discrimination of SARS-CoV-2. The proposed smartphone platform might establish a general method for visual detection of SARS-CoV-2 nucleic acid as well as other virus-related diseases. Graphical abstract
研究领域:
生物医学工程
·
分子生物学
·
诊断工具
·
病毒学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Pharmaceutical Analysis
·
2024
·
IF 6.1
Yue Liu,Taimei Cai,Sen Chen,Tao Wen,Hailong Peng
The overuse of antibiotics has led to the severe contamination of water bodies, posing a considerable hazard to human health. Therefore, the development of an accurate and rapid point-of-care testing (POCT) platform for the quantitative detection of antibiotics is necessary. In this study, Cerium oxide (CeO 2 ) and Ferrosoferric oxide (Fe 3 O 4 ) nanoparticles were simultaneously encapsulated into N-doped nanofibrous carbon microspheres to form of a novel nanozyme (CeFe-NCMzyme) with a porous structure, high surface area, and N-doped carbon material properties, leading to a considerable enhancement of the peroxidase (POD)-like activity compared with that of the CeO 2 or Fe 3 O 4 nanoparticles alone. The POD-like activity of CeFe-NCMzyme can be quenched using L-Cysteine (Cys) and subsequently restored by the addition of a quinolone antibiotic (norfloxacin, NOR). Therefore, CeFe-NCMzyme was used as a colorimetric sensor to detect NOR via an “On-Off” model of POD-like activity. The sensor possessed a wide linear range of 0.05-20.0 μM ( R 2 = 0.9910) with a detection limit of 35.70 nM. Furthermore, a smartphone-assisted POCT platform with CeFe-NCMzyme was fabricated for quantitative detection of NOR based on RGB analysis. With the use of the POCT platform, a linear range of 0.1-20.0 μM and a detection limit of 54.10 nM were obtained. The spiked recoveries in the water samples were ranged from 97.73% to 102.01%, and the sensor exhibited good accuracy and acceptable reliability. This study provides a portable POCT platform for the on-site and quantitative monitoring of quinolone antibiotics in real samples, particularly in resource-constrained settings.
研究领域:
环境科学
·
分析化学
·
生物技术
·
即时诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）