JOURNAL OF SEPARATION SCIENCE
·
2019
·Vol. 42
·期 21
·3372-3381
·
IF 2.516
Jin-Kui Ma,Xiao-Chen Huang,Shou-Lian Wei
A novel magnetic molecularly imprinted polymer adsorbing material was successfully synthesized to detect ribavirin in animal feedstuff. Molecularly imprinted polymer was prepared through surface polymerization by using ribavirin as template molecule, methyl methacrylate, and γ-methacryloxypropyl trimethoxy silane functionalized magnetic mesoporous silica as bifunctional monomers, and ethylene diglycidyl ether as crosslinking agent. The prepared magnetic molecularly imprinted polymer was characterized by scanning electron microscopy and infrared spectroscopy. Static and dynamic adsorption experiments and selective adsorption analysis were performed to evaluate the adsorption and selectivity of magnetic molecularly imprinted polymer. Different experiments were conducted to optimize the magnetic solid-phase extraction conditions. Under optimal experimental conditions, a magnetic molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography method was successfully developed for ribavirin detection. The established method achieved a satisfactory linear range of 0.20–50 mg/L ( R 2 > 0.99) and a low detection limit (0.081 mg/kg). An average recovery of 92–105% with relative standard deviation of <6.5% was obtained upon the application of the developed method to detect ribavirin in real feedstuff samples. Thus, established method can be used for the rapid and simple separation and detection of added ribavirin in feedstuff.
研究领域:
分析化学
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食品科学
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生物医学工程
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PubMed
高影响因子
Water
·
2026
·Vol. 18
·期 13
·1548
·
IF 3.5
Xicheng Wang,Junqi Jia,Zhangbin Pan,Congcong Li,Zhenqi Du,Ruibao Jia
Ribavirin (RBV) and chloroquine phosphate (CQP) are two typical pharmaceutical contaminants with distinct hydrophilic (RBV) and hydrophobic (CQP) properties. This polarity contrast led to markedly different degradation behaviors. Interestingly, hydrophobic CQP consistently degraded faster and with lower EEOthan hydrophilic RBV across all combined systems, highlighting pollutant polarity as a key determinant. This study systematically compared their degradation by three UV-based advanced oxidation processes (UV/AOPs): UV/H2O2, UV/PMS, and UV/KMnO4. Degradation kinetics, electrical energy per order (EEO), radical pathways, and water matrix effects were investigated. Sole UV or sole oxidant achieved negligible removal (<7.2%). All UV/AOPs greatly enhanced degradation in a dose-dependent manner. At equal molar oxidant concentration (0.2 mM), the efficiency order was UV/PMS > UV/H2O2≫ UV/KMnO4, with the gap widening at higher dosages. UV/H2O2exhibited the best overall performance, with remarkably low EEOvalues (0.59 kWh/m3for RBV and 0.46 kWh/m3for CQP at 0.2 mM), whereas UV/PMS showed faster kinetics but much higher energy consumption (e.g., 28.67 kWh/m3for RBV and 28.60 kWh/m3for CQP at 0.4 mM) and secondary pollution risks. UV/KMnO4had low energy but poor degradation. Radical quenching experiments revealed that in UV/H2O2, hydroxyl radicals (•OH) predominantly drove degradation regardless of pollutant polarity. In UV/PMS, •OH primarily drove RBV degradation, while CQP removal involved the combined action of •OH, sulfate radicals (SO4•−), and other reactive species. For the optimal UV/H2O2process, acidic pH (5.0) favored degradation; Cl−slightly promoted CQP removal but inhibited RBV, whereas SO42−, CO32−, and HCO3−suppressed both pollutants. Collectively, UV/H2O2is recommended as the most energy-efficient and robust UV/AOP for treating both hydrophilic and hydrophobic pharmaceuticals, with the additional insight that pollutant polarity governs both degradation kinetics and radical mechanisms.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Qing Gao,Dangqin Jin,Jiaqi Xu,Hao Huang,Huanren Cheng,Huaiguo Xue
Herein, we developed a highly sensitive imprinted electrochemical sensor for the trace detection of ribavirin (RBV) using pyrrole (PYR) and pyrro-1-propionyl-alaninoyl-chitooligosaccharides (PPACO) as bifunctional monomers on Prussian blue-gold nanocomposite films. PPACO had strong molecular effect on RBV molecule and was selected by quantitative calculations. After the deposition of the Prussian blue-gold nanocomposite on a glassy carbon electrode (GCE) surface, a 4-aminothiophenol layer successfully self-assembled on the surface. Subsequently, the molecularly imprinted membrane (MIM) was subjected to electrochemical polymerization on the electrode surface using RBV as the template and PPACO and PYR as the two monomers. After eluting the RBV molecules from the MIM, the fabricated RBV-MIM/Fn-Au-PB/GCE exhibited the specific adsorption of RBV. Under optimal conditions, differential pulse voltammetry (DPV) was used to measure the performance of the synthesized sensor, which exhibited a linear relationship between the decreasing peak current and RBV concentration from 0.015 to 3.5 μM with a low detection limit of 3 nM (S/N = 3). As a proof of concept, RBV-MIM/Fn-Au-PB/GCE was also applied to monitor the RBV content in RBV granules. It showed a satisfactory recovery (96.5–99.2%) with a relative standard deviation of less than 3.5% (n = 5), and thus, we believe it has potential for practical applications.
研究领域:
分析化学
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电化学传感器
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药物检测
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PubMed
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ANALYTICAL LETTERS
·
2019
·
IF 1.248
Xiaoyan Wang,Weiyang Shen,Xiaolei Zhang,Siyan Guo,Ye Gao,Xiaotong Li,Fang Feng,Gongjun Yang
A novel method for the indirect electrochemical determination of ribavirin based on boronic acid-diol recognition was developed using the platform as the sensing element. The device was constructed using a 3-aminophenylboronic acid (APBA)-electrochemically reduced graphene oxide (ERGO) modified electrode. When the electrode was immersed in a solution of ribavirin, complexation of boronic acid groups of APBA with ribavirin occurred at the surface of the electrode and simultaneously caused steric hindrance, resulting in a current decrease because the ferricyanide redox probe was unable access the surface. Under the optimized conditions, a linear relationship was obtained between the relative change in current (%Δi) of [Fe(CN)6]3−/4−and the concentration of ribavirin at levels from 10.0 to 7.50 × 102 ng mL−1. The proposed electrochemical sensor performed with acceptable sensitivity and reproducibility and was successfully used to determine the content of ribavirin in an injection with satisfactory results.
研究领域:
分析化学
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药物分析
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生物医学工程
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高影响因子
Fishes
·
2025
·Vol. 10
·期 3
·92
·
IF 2.4
Yijun Jia,Zhao Zhao,Leyang Chen,Yongqi Liu,Bin Zhu
A number of viral diseases have significantly impeded the growth of the aquaculture industry. Antiviral drugs represent an effective means of controlling infection. However, the efficacy of the entire therapeutic process is contingent upon the availability of an efficient delivery system. This study selected three common antiviral drugs and constructed corresponding drug delivery systems utilising single-walled carbon nanotubes (SWCNTs) as carriers. The reliability of carbon nanotubes as delivery carriers was evaluated by detecting the therapeutic effect on infectious splenic and renal necrosis virus (ISKNV). The findings demonstrated that SWCNTs can effectively enhance the absorption of the three drugs into the body, prolong their metabolic half-life, and improve the survival rate of fish infected with ISKNV. The Ribavirin-SWCNTs (RBV-SWCNTs) group exhibited the most pronounced protective effect, with a mortality rate of less than 25%. It was observed that SWCNTs facilitated the rapid transportation of ribavirin, with the drug content in the RBV-SWCNTs group being approximately double that of the free ribavirin group. Furthermore, this system markedly diminished the viral load, augmented enzyme activities, and elevated antiviral gene expression. This study indicated that carbon nanotubes are optimal carriers for antiviral drugs, which have considerable potential as a delivery vehicle for antiviral drugs to prevent viral infections in aquaculture.
研究领域:
水产养殖学
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病毒学
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药物递送
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Xiaoyan Chen,Jiangdong Cui,Xinyu Yang,Taoyue Yuan,Jiacheng Ge,Mengtao Ma,Shunke Ding,Wenhai Chu
The core bottleneck in water decontamination lies in trigging the selective removal of trace contaminants in the presence of complex water matrices, overcoming the inevitable consumption of oxidants and energy input by the water matrix. Here, the selective removal of phenolic contaminants with permanganate (PM) oxidation mediated by 2,2,6,6-tetramethylpiperidinooxy (TEMPO)-functionalized silica (SiO 2 @TEMPO) was firstly reported. SiO 2 @TEMPO was synthesized by covalently bonding 4-oxo-2,2,6,6-tetramethylpiperidinooxy onto SiO 2 through a 3-aminopropyltriethoxysilane bridge. The loading capacity of Wt% for nitrogen element in SiO 2 @TEMPO are 1.01–1.27 %. The degradation rate of tetracycline (around 1.50 min −1 ) with SiO 2 @TEMPO/PM are around 15 folds higher than that with PM, attributed to the formation of the reactive N-oxoammonium cation via single-electron transfer during PM oxidation. SiO 2 @TEMPO exhibited excellent stability and recyclability after five consecutive cycles of tetracycline degradation. Three transformation pathways (hydroxylation, demethylation, and dehydration) for tetracycline degradation by SiO 2 @TEMPO/PM were proposed based on the identification of 36 transformation products using HPLC/Q-Orbitrap HRMS. Thereinto, the attack of the N-oxoammonium cation in SiO 2 @TEMPO on the phenolic hydroxyl group to form corresponding hydroxylation and quinylation products accounted for the dominant degradation pathway. SiO 2 @TEMPO/PM also demonstrated remarkable adaptability across a wide pH range of 5.0–9.0, indicating its potential for practical applications. The significant resistance to the inhibitory effects of co-existing water matrix components (humic acid, cations and anions), along with the marked differences in degradation efficiency between phenolic and non-phenolic contaminants, demonstrate the selectivity of SiO 2 @TEMPO/PM towards phenolic contaminants. These results offer new insights into the selective removal of phenolic contaminants and pave the way for the development of efficient and sustainable strategies for water remediation.
研究领域:
环境科学
·
化学
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高影响因子
WATER RESEARCH
·
2024
·
IF 11.4
Haichuan Zhang,Jiawei Li,Shanshan Ye,Xiyang Zou,Rongxin Fei,Xinyu Hu,Ji Li
The electro-peroxone (EP) process encounters two inherent challenges in wastewater treatment: sluggish O 2 /O 3 transfer and substantial ozone waste. To overcome these limitations, we introduced micro-nano bubbles (MNBs) aeration to enhance O 2 /O 3 dissolution and diffusion, ultimately aiming to improve the removal of trace pharmaceutical contaminants from hospital wastewater. In the MNBs aeration system, the ozone transfer coefficient ranging from 0.536 to 0.265 min −1 , significantly surpassing that of conventional aeration (0.220 to 0.090 min −1 ) by approximately 2 to 4.5 times. Consequently, the EP process under MNBs aeration significantly enhanced ozone-resistant ibuprofen (IBU) removal, achieving a removal rate of 98.4 ± 1.5 %, far exceeding the 47.3 ± 4.7 % observed with conventional aeration. This significant improvement was attributed to the heightened production of hydroxyl radicals (•OH), reaching 0.97 × 10 −9 M s, compared to only 0.28 × 10 −9 M s in conventional aeration. The mechanism behind the enhanced •OH production in the MNBs-EP process relied primarily on two factors: improved O 2 /O 3 dissolution due to high internal pressure/large surface and enhanced O 3 /H 2 O 2 activation from high collapse energy. These factors together contributed to the robust oxidation capability of the MNBs-EP system. As a result, over 97 % removal efficiency was achieved for five representative pharmaceutical pollutants (sulfamethoxazole, ribavirin, norfloxacin, tetracycline and ampicillin) in just 1 min. Furthermore, when applied to real hospital wastewater, the MNBs-O 3 -E treatment system reduced all 15 detected trace pharmaceutical compounds to below 10 ng L −1 and achieved 14 types of pollutants with removal rates of over 85 % within 15 min, resulting in an ultrahigh total removal rate of 98.6 %, from an initial total concentration of 2108 ng L −1 to less than 30 ng L −1 . Thus, micro-nano aeration endowed the EP process as a promising advanced oxidation system for rapid and highly-effective removal of trace pharmaceutical contaminants from hospital wastewater.
研究领域:
环境科学
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水处理技术
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高级氧化工艺
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PubMed
高影响因子
Frontiers in Nutrition
·
2025
·
IF 5.1
Zhanding Cui,Jinlong Liu,Jinlong Wang,Jing Zhang,Yimei Cao,Kun Li,Zhixun Zhao,Hong Yuan,Xingwen Bai,Xueqing Ma,Pinghua Li,Yuanfang Fu,Huifang Bao,Dong Li,Qiang Zhang,Zaixin Liu,Kai Li,Tong Xu,Zengjun Lu
This study delves into the antiviral efficacy of Formononetin (FMN) and Mizoribine (MZR) against the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), a virus with a considerable economic impact and a current void in effective treatments. FMN and MZR were found to inhibit various PRRSV strains in vitro, predominantly in the early stages of viral infection. Noteworthy was the observation of their synergistic effects when combined with Ribavirin. The study underscores the antiviral potential of FMN and MZR, particularly emphasizing their low cytotoxicity at specific concentrations. These results position FMN and MZR as promising antiviral agents against PRRSV, underscoring their low cytotoxicity and efficacy in early-stage viral inhibition. Such findings pave the way for their potential inclusion in future PRRSV management strategies.
研究领域:
兽医学
·
病毒学
·
药理学
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PubMed
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Zilin Wu,Min Huang,Junmei Jiang,Chun Zhang,Gefei Hao,Moxian Chen,Qing X. Li,Mengao Jia,Junfeng Liu,Xiangyang Li
Ningnanmycin has been proven to effectively inhibit infection by potato virus Y (PVY), although its underlying mechanisms remain unclear. In this study, we report a novel finding that ningnanmycin affects PVY replication. Two approaches were employed: studies using PVY movement-deficient mutants suggest that ningnanmycin, at a concentration of 500 μg/mL, inhibits PVY replication. Ningnanmycin interacts with the PVY-encoded coat protein (CP) with a binding constant of 1.34 μmol/L, and key amino acids Glu168 and Thr206 are involved in this interaction. Additionally, ningnanmycin induces the expression of antiviral response genes in Nicotiana benthamiana, including PRXIIB, PRXIIE, PUB4, and PER42. Furthermore, studies revealed that the overexpression of PUB4 in N. benthamiana confers resistance to PVY infection. These findings highlight the mechanisms by which ningnanmycin activates the PUB4 gene and suppresses CP assembly, thereby inhibiting PVY in N. benthamiana. This study represents an important step toward elucidating the molecular mechanism underlying the antiviral activity of ningnanmycin.
研究领域:
植物病理学
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病毒学
·
分子生物学
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植物生物技术
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高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-12
·
IF 14.7
Cui Zhanding,Liu Jinlong,Xie Chong,Wang Tao,Sun Pu,Wang Jinlong,Li Jiaoyang,Li Guoxiu,Qiu Jicheng,Zhang Ying,Li Dengliang,Sun Ying,Yin Juanbin,Li Kun,Zhao Zhixun,Yuan Hong,Bai Xingwen,Ma Xueqing,Li Pinghua,Fu Yuanfang,Bao Huifang,Li Dong,Zhang Qiang,Liu Zaixin,Cao Yimei,Zhang Jing,Lu Zengjun
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) poses a major threat to the global swine industry, yet effective prevention and control measures remain elusive. This study unveils Nitazoxanide (NTZ) as a potent inhibitor of PRRSV both in vitro and in vivo. Through High-Throughput Screening techniques, 16 potential anti-PRRSV compounds are identified from a library comprising FDA-approved and pharmacopeial drugs. We show that NTZ displays strong efficacy in reducing PRRSV proliferation and transmission in a swine model, alleviating viremia and lung damage. Additionally, Tizoxanide (TIZ), the primary metabolite of NTZ, has been identified as a facilitator of NMRAL1 dimerization. This finding potentially sheds light on the underlying mechanism contributing to TIZ’s role in augmenting the sensitivity of the IFN-β pathway. These results indicate the promising potential of NTZ as a repurposed therapeutic agent for Porcine Reproductive and Respiratory Syndrome (PRRS). Additionally, they provide valuable insights into the antiviral mechanisms underlying NTZ’s effectiveness.
研究领域:
兽医学
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分子生物学
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药理学
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病毒学
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治疗学
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Yucan Zhang,Chenxi Shi,Jin Yuan,Yue Zhang,Xiaohui Jin,Shaopo Zu,Honglei Zhang,Hui Hu
Porcine deltacoronavirus (PDCoV) is an emerging enteropathogenic coronavirus that mainly causes diarrhea and death in suckling piglets and also has the potential for cross-species transmission, threatening public health. However, there is still no effective vaccine or drug to prevent PDCoV infection. In order to accelerate the development of antiviral drugs, we established a high-throughput screening platform using a novel genome editing technology called transformation-associated recombination cloning in yeast. The recombinant PDCoV and PDCoV reporter virus expressing enhanced green fluorescent protein were both rapidly rescued with stable genealogical characteristics during passage. Further study demonstrated that the reporter virus can be used for high-throughput screening of antiviral drugs with a Z-factor of 0.821–0.826. Then, a medicine food homology compound library was applied, and we found that three compounds were potential antiviral reagents. In summary, we have established a fast and efficient reverse genetic system of PDCoV, providing a powerful platform for the research of antiviral drugs.
研究领域:
病毒学
·
分子生物学
·
药理学
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生物技术
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PubMed
高影响因子
Cell Host & Microbe
·
2026
·
IF 23.2
Fengqing Lin,Lianglan Li,Yuanpeng He,Shujie Xu,Qiufen Mo,Wangsen Cao,Aikun Fu,Weiqin Li
The enteric microbiome and nutrient sensing within the small intestine play critical roles in maintaining host metabolic homeostasis. Although various bacteria and some fungi have established functions in nutrient metabolism, the role of the enteric virome remains poorly understood. Here, we demonstrate that the enteric virome significantly influences carbohydrate digestion and absorption independently of the bacteriome. Furthermore, the virome elicits distinct responses across different intestinal cell types. Specifically, it activates programs for carbohydrate digestion and absorption in intestinal epithelial cells while simultaneously stimulating antigen-presenting cells―Th17 cells―to produce interleukin-22, a cytokine that curbs excessive carbohydrate uptake. The virome’s effect on carbohydrate digestion and absorption—whether suppressive or stimulatory—depends on the presence or absence of immune surveillance. This intricate interplay between metabolic and immune pathways establishes the enteric virome as a pivotal regulator of metabolism and reveals the virome’s intrinsic capacity to autonomously modulate vertebrate intestinal physiology.
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高影响因子
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