BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Hong Ke,Haifeng Sha,Yinfang Wang,Weiwei Guo,Xin Zhang,Zhiming Wang,Chusen Huang,Nengqin Jia
Electrochemiluminescent (ECL) assay has gradually drawn increasing interest in the biomedical analysis. This paper proposed a new methodology for ultrasensitive and facile detection of Alzheimer's disease marker β-amyloid (Aβ) by fabricating a sandwich-type ECL sensing platform. Herein, electrochemiluminescence resonance energy transfer (ECL-RET) was employed to determine Aβ concentration, which can be attributed to the quenching effect from RET between Ru(bpy) 3 2+ and gold nanorods (GNRs) acting as ECL-RET electron donor and acceptor, respectively. In this protocol, mesoporous carbon nanospheres were adopted to immobilize ECL reactant Ru(bpy) 3 2+ and antibody via nafion to acquire the RET donor nanocomposites (MOCs/nafion/Ru(bpy) 3 2+ /antibody), which were tightly interconnected with epoxy group functionalized Fe 3 O 4 nanoparticles . It is of vital importance that GNRs with exquisite rod shape were synthesized and exhibited a typical absorption peak at 650 nm to quench ECL signal of Ru(bpy) 3 2+ effectively. In addition, the ECL emission decreased linearly with the logarithm of Aβ concentration in a wide linear range from 1.0 × 10 −5 to 100 ng/mL with a detection limit of 4.2 × 10 −6 ng/mL. Furthermore, distinctive and desirable properties were verified to declare the promise for being applicable to analyze the Aβ content in real Alzheimer's cerebrospinal fluid samples with satisfactory results.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
·
诊断学
·
神经退行性疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2021
·Vol. 37
·期 1
·58-71
·
IF 2.464
Zheng Chang,Chao Zhang,Binghua Yao
A novel dual-sensitization electrochemiluminescence (ECL) immunosensor for the detection of tumour protein prostate specific antigen (PSA) at trace level using Ru(bpy) 3 2+ -doped chitosan/SiO 2 nanoparticles (Ru(bpy) 3 2+ /chitosan/SiO 2 NPs) as the first signal enhancers was fabricated. Due to chitosan with excellent pore forming capacity, these nanoparticles possess porous structures and better photopermeability, and therefore have higher luminescence efficiencies compared with Ru(bpy) 3 2+ /SiO 2 NPs reported in previous publications. Conversely, chitosan with good biocompatibility and high hydrophilicity was electrochemically decorated onto a Nafion/multiwall carbon nanotubes (Nafion/MWNTs) modified electrode surface and used as the second sensitizing matrix to seize large amounts of prostate specific capture antibody (Ab 1 ). The chitosan-decorated Nafion/MWNTs composites exhibited a 5.5-times higher ECL intensity than the unadorned Nafion/MWNTs films. Also, without additional reagents, such as (3-aminopropyl)triethoxysilane (APTS), the one-step functionalized Ru(bpy) 3 2+ /chitosan/SiO 2 NPs provided a large number of active arms to connect with PSA-detected antibodies (Ab 2 ) through the amino groups in chitosan. After a sandwich immunoreaction, the PSA antigen and Ru(bpy) 3 2+ /chitosan/SiO 2 NPs-labelled Ab 2 were sequentially captured onto the Ab 1 /chitosan/Nafion/MWNTs-modified electrode surface. The ECL signal increases were linearly related to the PSA antigen concentrations and ranged from 0.01 pg·mLl −1 to 10.0 pg·mLl −1 . Under the optimized experimental conditions, the immunosensor displayed excellent sensitivity and selectivity. The detection limit was as low as 3.4 fg·mLl −1 , equivalent to, or better than, those of the reported ECL immunosensors for PSA.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Xueyuan Li,Xuezhong Du
Surface enhanced electrochemiluminescence (SEECL) emission of the Ru(bpy) 3 2+ (bpy = 2,2′-bipyridyl)/tripropylamine (TPA) system by [email protected] 2 core/shell nanoparticles (NPs) was achieved owing to the electromagnetic field enhancement induced by localized surface plasmon resonance (LSPR) of internal Au cores. The Ru(bpy) 3 2+ /TPA system in electrolyte solution showed a maximum ECL enhancement when a glassy carbon electrode (GCE) was modified with [email protected] 2 NPs ( [email protected] 2 /GCE; Au core, ca. 68 nm; silica shell, 12 nm). The ECL emission of the system in the case of [email protected] 2 /GCE was 29 fold stronger than the ECL emission in the case of bare GCE, ca. 39 fold stronger than the ECL emission in the case of hollow silica NP-modified GCE, and 100 fold stronger than the ECL emission in the case of AuNP-modified GCE. To the best of our knowledge, the respective folds of ECL enhancement in the Ru(bpy) 3 2+ /TPA system by the LSPR-induced electromagnetic field of AuNPs are the largest reported to date. The ECL emission of TPA •+ radical cations was detected for the first time taking the advantage of the electromagnetic field enhancement. A SEECL platform based on the [email protected] 2 /GCE in the Ru(bpy) 3 2+ /TPA system was constructed for the detection of dopamine (DA). This is the first example that the AuNP-enhanced ECL emission was quenched by DA for the selective detection of DA, and a wide linear range of 0.01–600 μM directly relevant to DA concentration was obtained with a low detection limit of 0.004 μM and high sensitivity and accuracy. The mechanism that the ECL emission of the Ru(bpy) 3 2+ /TPA system was quenched by DA for the detection of DA was clearly elaborated. The level of DA in human serum was determined using the SEECL platform.
研究领域:
分析化学
·
电化学
·
纳米技术
·
生物传感器
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2022
·Vol. 24
·期 1
·1-13
·
IF 2.533
Lu Yifei,Wang Sui,Guo Zhiyong,Hu Yufang
A ratiometric fluorescence sensor (c-CDs/Ru(bpy) 3 2+ ) based on the selective recognition of chitosan carbon dots (c-CDs) on ClO − and combine with Ru(bpy) 3 2+ was established for sensitive and visual monitoring of hypochlorite ion (ClO − ). Using this nanosensor, a highly sensitive assay of ClO − in the linear range of 0.026 − 70 μM with a low detection limit (LOD) of 8.6 nM was provided by quenching the blue emission fluorescence. It is more attractive that ClO − can also be visualized by this nanosensor via evident color changes of the solution (from blue to red) under an ultraviolet (UV) lamp. In addition, since c-CDs and Ru(bpy) 3 2+ have good water dispersibility and water solubility, respectively, the sensor can be applied to the detection of HClO in 100% aqueous solution. This is a new colorimetric and fluorescent dual-mode nanosensor with more simple preparation procedures, low cost, and excellent biocompatibility. It provides a new method for detecting ClO − and promotes the development of biosensing.
研究领域:
分析化学
·
纳米技术
·
生物传感器
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Jiawei Gong,Tongtong Zhang,Peng Chen,Fei Yan,Jiyang Liu
Herein we reported the construction of dual-mode electrochemiluminescence (ECL) and electrochemical (EC) sensing platform using the bipolar silica nanochannel array (bp-SNA) confined with the bifunctional probe (tris(2,2′-bipyridyl) ruthenium , Ru(bpy) 3 2+ ) inside the nanochannels (designated as Ru@bp-SNA). With the asymmetric surface charges, bp-SNA on the indium tin oxide electrode can effectively function as electrostatic nanocage for the stable confinement of Ru(bpy) 3 2+ via dual electrostatic force , namely electrostatic attraction of inner negative surface and electrostatic repulsion of outer positive surface. Ru(bpy) 3 2+ immobilized into the nanospace acts as both highly efficient ECL luminophore and well-defined EC redox molecule to generate strong signal. Since the modification of bio-recognition units on the top of Ru@bp-SNA is able to confer excellent recognitive capacity and obtain the formation of complex in the presence of specific analyte, the decrease of ECL and EC signals enables the quantitative detection of this analyte. As proof-of-concept experiments, two tumor related biomarkers, CEA and CA15–3, have been successfully determined in aqueous solution and human serum by ECL and EC modes, respectively.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
生物传感器
·
癌症研究
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xinxia Wang,Wei Yuan,Zhilan Sun,Fang Liu,Daoying Wang
Pseudomonas aeruginosa ( P. aeruginosa ) is an opportunistic infection-causing pathogen that threatens human health. Accordingly, a rapid and ultrasensitive analytical method is required urgently. Herein, an ultrasensitive multicolor electrochromic sensing platform was established on the basis of a closed bipolar electrode (BPE). Prussian blue (PB), the blue spot that can be easily electrodeposited, was selected as an electrochromic indicator at the closed BPE cathode. Integrating with the anodic emitter, Ru(bpy) 3 2+ , which emitted optical red light, visualized multicolor electrochromism was achieved on closed BPE. Particularly, physical separation between the positive and negative poles of the closed BPE greatly prevented mutual interference between Ru(bpy) 3 2+ and PB. Consequently, the sensitivity and accuracy of the proposed biosensor considerably improved. Notably, owing to magnetic-separation technology, the closed BPE surface required no modification. Without any complex pretreatment, the entire experiment time could be greatly shortened because the [email protected] completely captured P. aeruginosa in food matrix within only 20 min. By comparing the visual electrochromic colors, ultrasensitive screening of P. aeruginosa was accomplished within 1–10 8 CFU mL −1 . Combining the merits of closed BPE, electrochemiluminescence (ECL), and electrochromic, this strategy provided an accurate and intrinsic way for visual detection of P. aeruginosa , as well as great potential in measuring other pathogens.
研究领域:
生物技术
·
分析化学
·
微生物学
·
传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2019
·Vol. 23
·期 8
·2579-2588
·
IF 2.531
Du Fangkai,Zhang Hui,Tan Xuecai,Ai Chenhao,Li Mengru,Yan Jun,Liu Min,Wu Yeyu,Feng Defen,Liu Shaogang,Han Heyou
A sensitive electrochemiluminescence (ECL) aptasensor based on 3D graphene is described for thrombin detection utilizing nitrogen-doped graphene quantum dots doped silica (NGQDs@SiO 2 ) nanocomposite labeling as an enhancer. Ru(bpy) 3 2+ -Pt nanoparticles (Ru-PtNPs) with an ECL signal were dropped onto a 3D graphene-modified electrode, and then, a complementary thrombin aptamer (cDNA) was conjugated to the Ru-PtNPs. Finally, NGQDs@SiO 2 , coupled with the thrombin aptamer as the booster, was attached to the electrode surface by using hybridization with the cDNA, and the ECL intensity was efficiently enhanced. The target thrombin induced the NGQDs@SiO 2 nanocomposites labeled with the thrombin (TB) aptamer to be extracted because of their strong binding with thrombin. The immobilization amount of NGQDs@SiO 2 on the modified electrode decreased. Thus, the presence of thrombin could result in a decrease in the ECL signal. On the basis of the signal amplification factors, the fabricated ECL aptasensor demonstrated excellent properties with concentrations varying from 2.0 pM to 50 nM and a detection limit of 23.1 fM.
研究领域:
材料科学
·
分析化学
·
生物传感器
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Jing Zhang,Dan Xu,Zijie Deng,Xueping Tan,Dongnan Guo,Yanru Qiao,You Li,Xiaofang Hou,Sicen Wang,Junbo Zhang
A thrombin-activity-based electrochemiluminescence (ECL) biosensor was successfully constructed using tungsten oxide quantum dots (WO 3-x QD S ) as the co-reactant and thrombin-cleavable peptides as the recognizer. Specifically, Ru(bpy) 3 2+ were doped on silica nanoparticles (Ru@SiO 2 ), which greatly enhanced the ECL potential. AuNPs@WO 3-x QDs composite was then prepared to accelerate electron transfer and improve the ECL signal by 219 times. Under ideal conditions, the limit of detection for thrombin in serum was determined to be 0.28 μU/mL with a linear range from 1 μU/mL to 1 U/mL. In addition, the developed ECL biosensor was used to screen for thrombin inhibitors from 12 compounds in Artemisiae Argyi Folium. Among the compounds tested, it was observed that 100 μmol/L luteolin exhibited a significantly higher inhibition rate (exceeding 80%) compared to apigenin , isorhamnetin , naringin , or eriodictyol . In an in-vitro anticoagulation experiment, luteolin (100 μmol/L) prolonged APTT by 49%, and the molecular docking assay indicated that luteolin had binding sites of Gly219 and Asp189 in the active pockets of thrombin. This may have been the main reason underpinning luteolin's anticoagulation effects. Overall, the Ru@WO 3-x QD S ECL biosensor provided a reliable strategy for thrombin activity assay and screening of anticoagulant agents.
研究领域:
分析化学
·
生物化学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Yu-Ling Wang,Fu-Rao Liu,Jun-Tao Cao,Shu-Wei Ren,Yan-Ming Liu
The identification of tumor markers is of great importance for clinical diagnosis but accurate detection with high sensitivity is still a great challenge. In present work, a spatial-resolved dual-signal-output electrochemiluminescent (ECL) ratiometric assay platform was constructed for sensitive detection of prostate specific antigen (PSA) on a dual-disk glassy carbon electrode. To fabricate the platform, flower-like CdS three-dimensional (3D) assemblies and Ru(bpy) 3 2+ -conjugated silica nanoparticles (Ru(bpy) 3 2+ @RuSi NPs), were immobilized onto the two disks as cathodic and anodic ECL emitters, respectively. After the stepwise modification of the gold nanoparticles , antibody for PSA, and bovine serum albumin onto the two disks respectively, the Ru(bpy) 3 2+ @RuSi NPs-based disk were incubated with varied concentration of PSA as working electrode , whereas the flower-like CdS 3D assemblies-based disk with fixed concentration of PSA were taken as internal reference electrode . The label free assay of PSA was realized by the ratio of anodic ECL signal from working electrode to the cathodic ECL signal from the internal reference electrode (ECL anode /ECL cathode ). On the basis of the spatial-resolved dual-signal-output ratiometric ECL sensor, the PSA can be detected accurately with a linear range of 0.001 – 50 ng/mL at a concentration as low as 0.34 pg/mL. Furthermore, the proposed method was applied for PSA determination in human serum samples with satisfying results. Thanks to the same modified process of the two disks, this universal design well avoids environmental errors including the interference caused in the biological recognition process, which effectively reduces the false positive or negative errors, exhibiting a greatly improved accuracy, reliability and sensitivity.
研究领域:
分析化学
·
生物化学
·
生物医学工程
·
临床诊断
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Libo Li,Bainian Chen,Xiaohong Liu,Panao Jiang,Lijun Luo,Xia Li,Tianyan You
Divalent mercury ion (Hg 2+ ) is widely distributed in the ecological environment and harmful to the human body and the environment. Herein, a highly sensitive and selective ‘on–off-on’ electrochemiluminescence (ECL) aptamer sensing method based on the dual signal amplification strategy of a self-enhanced luminophore and ECL resonance energy transfer (ECL-RET) was developed to detect Hg 2+ . In detail, the first ‘switch-on’ state was obtained by the self-enhanced luminophore of Ru(bpy) 3 2+ -doped silica nanoparticle-nitrogen-doped graphene quantum dots ( [email protected] 2 -NGQDs) modified glassy carbon electrode, which significantly amplified the ECL intensity compared with the mixture of [email protected] 2 and NGQDs . Later, the ‘switch-off’ state was induced by gold nanoparticles (AuNPs), which acted the acceptor in the ECL-RET system between AuNPs and [email protected] 2 -NGQDs due to their good spectral overlap. This combination of the self-enhanced luminophore and ECL-RET provided a novel dual signal amplification strategy, which led to a high signal-to-noise ratio and high analysis sensitivity. Finally, upon the addition of Hg 2+ , the formation of a stable T-Hg 2+ -T structure induced the release of AuNPs from the sensing interface and halted the ECL-RET process, resulting in ECL signal recovery (the second ‘switch-on’ state). For the detection of Hg 2+ , the proposed ECL sensor exhibited a wide linear range from 1 × 10 −14 to 1 × 10 −6 M and a highly sensitive detection limit of 3.0 fM. Finally, the feasibility of the developed method in water sample analysis was investigated.
研究领域:
分析化学
·
环境科学
·
材料科学
·
纳米技术
·
传感器技术
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2020
·
IF 5.339
Libo Li,Bainian Chen,Lijun Luo,Xiaohong Liu,Xiaoya Bi,Tianyan You
In the present study, a novel self-enhanced electrochemiluminescence (ECL) aptasensor which combined self-enhanced ECL composite as signal response element and aptamer as the specific recognition element was firstly proposed for the sensitive and selective detection of Hg 2+ . Innovatively, the luminophore Ru(bpy) 3 2+ -doped silica nanoparticles functionalized with 3-aminopropyltriethoxysilane (NH 2 – [email protected] 2 ) and the co-reactant nitrogen doped graphene quantum dots (NGQDs) were bound together to form the self-enhanced ECL composite, NH 2 – [email protected] 2 -NGQDs, by electrostatic adsorption. Satisfactorily, high and stable luminous efficiency of NH 2 – [email protected] 2 -NGQDs was obtained benefited from the short electron transfer distance and low energy loss of self-enhanced-typal ECL composite. For the fabrication of the self-enhanced ECL aptasensor for Hg 2+ detection, the Hg 2+ aptamer, of which one end was connected to NH 2 – [email protected] 2 -NGQDs, and the other end was fixed on glass carbon electrode (GCE) surface through Au–S bond, was served as a bridge. Upon the addition of Hg 2+ , the aptamer was bent due to the formation of thymine-Hg 2+ -thymine (T-Hg 2+ -T) specific structure, which caused the self-enhanced ECL composite was close to the GCE surface. On this basis, a linearly enhanced ECL signal was acquired with the concentration of Hg 2+ in the range of 5.0 × 10 −11 M − 1.0 × 10 −6 M with excellent selectivity , repeatability and stability in 3 min for each assay. In addition, the proposed aptasensor showed satisfying accuracy and practicability for Hg 2+ analysis in tap and canal water verified by the inductively coupled plasma-mass spectrometry (ICP-MS) method.
研究领域:
分析化学
·
环境科学
·
纳米技术
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）