International Journal of Electrochemical Science
·
2023
·
IF 1.5
Nian Lu,Wen-chang Wang,Xiao-hui Chen,Zhi-dong Chen
In this paper, a PSA/Ru(bpy) 3 2+ modified electrode was prepared by electrochemical polymerization and used as the working electrode in ECL detection of melamine. Under the optimal conditions, the sensor shows a wide linear range of 1.0×10 -7 ~1.0×10 -5 mol/L, detection limit of 2.5×10 -8 mol/L with good reproducibility and stability. The method is expected to be a new method for the detection of melamine, which is simple and convenient.
研究领域:
分析化学
·
食品科学
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DOI
高影响因子
TALANTA
·
2012
·
IF 3.794
Baomei Huang,Xibin Zhou,Zhonghua Xue,Guofan Wu,Jie Du,Di Luo,Tao Liu,Jing Ru,Xiaoquan Lu
Efficient and stable quenching of electrochemiluminescence of Ru(bpy) 3 2+ /TPA by malachite green(MG) and crystal violet(CV) at the glass carbon (GC) electrode is reported. A novel quenching mechanism has been proposed. Resonance energy transfer from the excited-state luminophore Ru(bpy) 3 2+⁎ to MG/CV and dynamic quenching are suggested as the mechanism for quenching ECL. The quenching mechanism is discussed in detail based on UV–visible absorption spectra, cyclic voltammograms, ECL curves and fluorescence methods . MG shows more efficient quenching than CV. Moreover, the quenched ECL intensity versus the concentration of MG and CV are linear over the concentration ranges of 8×10 −10 –8×10 −7 M and 3.46×10 −9 –5.5×10 −7 M, respectively. The corresponding limit of detection (LOD) was 1.0×10 −10 M for MG and 1.1×10 −10 M for CV ( S / N =3).
研究领域:
分析化学
·
电化学
·
发光光谱学
·
传感器与生物传感器
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DOI
高影响因子
ANALYTICAL SCIENCES
·
2019
·Vol. 35
·期 6
·639-644
·
IF 1.618
Shan Xueling,Pan Yuting,Chen Xiaohui,Wang Wenchang,Chen Zhidong
A novel electrochemiluminescence (ECL) sensor for detection of brilliant blue FCF (BB) has been developed using Ru@ Zn-MOF/nafion modified GCE (glass carbon electrode) in this research. Different from conventional method for usage of Ru(bpy) 3 2+ in solution-phase, Ru(bpy) 3 2+ here was immobilized on a zinc-metal-organic-framework (Zn-MOF). After adding BB, a significant quenching phenomenon of ECL intensity was observed. The behavior of BB on the quenching effect of Ru(bpy) 3 2+ /Zn-MOF in different conditions was investigated thoroughly and the detection limit was achieved to 2.5 × 10 −8 M in an optimized condition. Furthermore, the interference of some conventional ions and amino acids to the detection of BB was also investigated. Additionally, the composite showed a good effect on the detection of BB in commercial samples. The proposed sensor provided a promising platform for food safety analysis, environmental monitoring and clinical testing.
研究领域:
分析化学
·
食品安全
·
环境监测
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产品关联
DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2018
·Vol. 30
·期 2
·025501
·
IF 3.404
Danqing Liu,Tianchan Li,Weicheng Huang,Zhuang Ma,Wei Zhang,Rui Zhang,Hong Yan,Bin Yang,Shaoqin Liu
Foodborne pathogens are perpetual threats to human and animal health. Detection of pathogens requires accurate, sensitive, rapid and point-of-care diagnostic assays. In this study, we described a simple and sensitive electrochemiluminescent (ECL) assay to detect the deadly bacteria Escherichia coli O157:H7 by {\rm{Ru}}{{({\rm{bpy}})}_{3}}^{2+}-coated ZnO nanorods arrays (NAs). The {\rm{Ru}}{{({\rm{bpy}})}_{3}}^{2+}-coated ZnO NAs were fabricated by immobilizing {\rm{Ru}}{{({\rm{bpy}})}_{3}}^{2+} on ZnO NAs with a large specific surface area and good conductivity. An {\rm{Ru}}{{({\rm{bpy}})}_{3}}^{2+}-2-(dibutylamino)-ethanol (DBAE) system coated on ZnO NAs exhibits high ECL intensity, rapid response and good stability. This system was further developed as an ECL immunosensor used in the detection of E. coli O157:H7. The proposed ECL immunosensor exhibits a broad detection range within the scope of 200–100 000 CFU ml−1 and quite a low detection limit of 143 CFU ml−1. The high specificity, remarkable reproducibility and good stability offer a sensitive, selective, and convenient pathway for detecting E. coli O157:H7 in the field of food safety and clinical diagnosis.
研究领域:
食品安全
·
临床诊断
·
微生物学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Yufeng Liu,Min Zhou,Wen Cao,Xiaoyu Wang,Quan Wang,Sirong Li,Hui Wei
Nanomaterials with enzyme-like characteristics (nanozymes) have been developed to mimic enzymes because of their low cost, high stability, and large-scale production. By using light as an external stimulation, one can modulate nanozymes’ catalytic activities with controlled spatial and temporal precision. A few inorganic photoactive materials have been investigated to construct light-responsive oxidase-like nanozymes. However, these materials suffered from limited absorbance of visible light. To address this challenge, herein we have developed a photosensitized metal–organic framework (PSMOF) by using a derivative of Ru(bpy)32+ with stronger visible-light absorption as a PS linker. The PSMOF exhibited excellent oxidase-like activity, which could be modulated by switching light on and off. Moreover, the PSMOF was used to detect glutathione levels in both normal and cancer cells with good selectivity and high sensitivity. This study not only provides a smart strategy to modulate nanozymes’ activities but also broadens the sensing applications of nanozymes.
研究领域:
材料科学
·
纳米技术
·
生物化学
·
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Yizhuo Fu,Jingke Pan,Yuhao Liu,Chao Lu
The electrochemiluminescence (ECL) effectiveness of the tris(bipyridine) ruthenium(II) (Ru(bpy)32+) system is hampered by aggregation-caused quenching (ACQ) in optoelectronic systems as a result of π–π accumulation of the aromatic ring structure. In this work, a negatively charged tetraphenylvinyl molecule (TPE-2SO3Na, TPE-4SO3Na) was synthesized to modify the electrode interface, and the π–π accumulation between Ru(bpy)32+ molecules was transformed into the π–π interaction between Ru(bpy)32+ and TPE molecules. Interestingly, the ECL signal intensity of the Ru(bpy)32+-tripropylamine (TPA) system in the presence of TPE-2SO3Na was increased by about 15 times due to the π–π action and electrostatic action. In comparison with traditional physical packaging with porous zeolites, metal–organic frameworks (MOFs), and covalent organic frameworks (COFs), the fabricated electrode interface modification strategy was simple and efficient to avoid π–π accumulation in aqueous solutions. Our success will inspire other researchers to investigate the supramolecular interaction (π–π interaction, electrostatic interaction, hydrophilic interaction, and host–guest interaction) at the electrode interface to amplify the ECL intensities of Ru(bpy)32+.
研究领域:
材料科学
·
电化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2012
·
IF 3.898
Tingting Hao,Zhiyong Guo,Shuping Du,Lili Shi
An ultrasensitive electrochemiluminescence (ECL) immunosensor for the detection of carcinoembryonic antigen (CEA) in serum and saliva was developed. Ru(bpy) 3 2+ -graphene-Nafion composite was first coated on the glassy carbon electrode surface in this ECL immunosensor. The sandwich-type immunoreactions between the first antibody and the second antibody bridged the donors Ru(bpy) 3 2+ and acceptors QDs , which led to the occurrence of ECL quenching of Ru(bpy) 3 2+ by QDs . Based on the efficient ECL quenching, ultrasensitive CEA detection was realized. Under optimal conditions, the ECL signal depended linearly on the logarithm of the CEA concentration within a range from 0.005 to 0.5 pg/mL, and the detection limit of CEA was 0.002 pg/mL. Moreover, the proposed ECL immunosensor provides a stable, specific, and highly sensitive immunoassay protocol for CEA detection at very low levels, which might hold a promise for clinical application.
研究领域:
分析化学
·
生物医学工程
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DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2025
·
IF 4.1
Ziqi Wang,Yahui Ji,Nana You,Xiaoping Hu,Fangxin Du,Gen Liu
Testing ribavirin (RBV) is essential to promote its appropriate clinical use, mitigate resistance development, reduce adverse effects, and safeguard public health. Electrochemiluminescence (ECL) outperforms conventional methods in RBV detection, offering superior sensitivity and reduced background noise. In this work, ECL micro-reactors were prepared by incorporating Ru(bpy) 3 2+ into a mesoporous silica matrix with two-dimensional hexagonal structure (SBA-15). In this configuration, tripropylamine (TPA) was employed as a co-reactant, which interacted with Ru(bpy) 3 2+ to enhance the ECL signal. The quenching effect of ribavirin (RBV) on the Ru(bpy) 3 2+ -TPA system was confirmed through its significant suppression of ECL emission. A glassy carbon electrode (GCE) modified with SBA-15 loaded with Ru(bpy) 3 2+ (SBA-15@Ru) was fabricated as a novel ECL sensor. This sensor enabled the detection of RBV over a wide concentration range 1.0 × 10 −9 mol·L −1 to 1.0 × 10 −5 mol·L −1 , achieving a detection limit (LOD) of 3.33 × 10 −10 mol·L −1 . Additionally, the sensor exhibited outstanding stability and selectivity and was successfully applied for accurate quantification of RBV in pharmaceutical samples.
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产品关联
DOI
高影响因子
ANALYTICAL LETTERS
·
2024
·
IF 1.6
Jiali Ju,Qiaoyu Ding,Jianwei Xie,Guixin Li
A graphene oxide-Ru(bpy)32+-polyaniline-nanocomposite (GO-Ru(bpy)32+-PANI) was prepared to develop a sensitive label-free electrochemiluminescence (ECL) immunosensor for mucoprotein 1 (MUC1). Ru(bpy)32+ and PANI were decorated together on graphene oxide for the first time. The GO-Ru(bpy)32+-PANI material was characterized by scanning electron microscopy (SEM), absorption spectroscopy, Fourier transform infrared spectroscopy (FTIR), fluorescence, electrochemical impedance spectroscopy (EIS), and ECL. The GO-Ru(bpy)32+-PANI exhibited excellent stability. The ECL immunosensor for MUC1 was fabricated by covalently immobilizing anti-MUC1 upon GO-Ru(bpy)32+-PANI modified indium-doped tin oxide (ITO) electrode. In the presence of MUC1, a decrease of ECL was observed due to the formation of the anti-MUC1/MUC1 complex. The immunosensor showed a linear relationship from 1 × 10−13 to 1 × 10−7 mg/mL with a detection limit of 8.2 × 10−14 mg/mL. The immunosensor was applied to the determination of MUC1 in human serum.
研究领域:
生物医学工程
·
分析化学
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产品关联
DOI
高影响因子
ChemElectroChem
·
2020
·Vol. 7
·期 20
·4239-4244
·
IF 4.154
Fan Yuan,Mohamed Ibrahim Halawa,Xiangui Ma,Abubakar Abdussalam,Baohua Lou,Guobao Xu
Graphical Shine and detect : Oxamic hydrazide (OHA) can dramatically increase the electrochemiluminescence (ECL) intensity of Ru(bpy) 3 2+ on glassy carbon electrode (GCE). 4-Nitrobenzaldehyde (4-NBA) can react with OHA to form a hydrazone product through nucleophilic addition, inducing a significant quenching on the ECL intensity of the developed Ru(bpy) 3 2+ /OHA system. Based on this, a sensing platform of 4-NBA was constructed. Oxamic hydrazide (OHA) was utilized as an efficient coreactant of Ru(bpy) 3 2+ for the first time, which can dramatically increase the electrochemiluminescence (ECL) intensity of Ru(bpy) 3 2+ on glassy carbon electrode (GCE). 4-nitrobenzaldehyde (4-NBA), a nitroaromatic compound, has mutagenic and carcinogenic toxicity on humans′ genome and causes a serious impact on the environment. Hence, the detection of its level in biological and environmental samples is of great significance. Interestingly, 4-NBA was found to induce a dramatic quenching for the ECL intensity of the developed Ru(bpy) 3 2+ /OHA system owing to the formation of hydrazone product through the nucleophilic addition reaction with OHA. Under optimal conditions, the ECL decreasing value of Ru(bpy) 3 2+ /OHA in the presence of 4-NBA is linear in the range of 20 μM to 800 μM with a limit of detection (LOD) of 1.09 μM (signal-to-noise 3 : 1). Finally, this strategy enabled the detection of 4-NBA in tap water with satisfactory recoveries from 97.84 % to 103.56 %.
研究领域:
分析化学
·
环境科学
·
毒理学
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产品关联
DOI
高影响因子
ELECTROCHEMISTRY COMMUNICATIONS
·
2011
·
IF 4.287
Kai Zeng,Manli Guo,Yingjun Zhang,Meng Qing,Afang Liu,Zhou Nie,Yan Huang,Yuliang Pan,Shouzhuo Yao
A novel method based on click chemistry for the immobilization of Ru(bpy) 3 2+ derivative on electrode to fabricate a solid state electrochemiluminescence (ECL) sensor was reported. Pentenyl functionalized Ru(bpy′) 3 Cl 2 was synthesized and immobilized on a (3-mercaptopropyl) trimethoxysilane (MPTMS) pretreated indium tin oxide (ITO) substrate via thiol-ene click chemistry reaction between its alkenyl group and the sulfhydryl group of MPTMS. The immobilization method is simple, fast, and efficient. More importantly, the resulting Ru(bpy′) 3 2+ monolayer film exhibited excellent stability in organic solvent, which is superior to many previously reported methods. Using tri- n -propylamine (TPrA) as a model analyte, the ECL sensor gave a wide linear range over 5 × 10 − 6 to 5 × 10 − 3 M, with a satisfactory detection limit of 1 × 10 − 6 M ( S / N = 3).
研究领域:
分析化学
·
材料科学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2015
·
IF 4.513
Zhenyu Zhou,Weiliang Guo,Linru Xu,Qian Yang,Bin Su
In this paper, we report significantly enhanced electrochemiluminescence (ECL) of tris(2,2′-bipyridyl)ruthenium(II) ( Ru ( bpy ) 3 2 + ) at indium tin oxide (ITO) electrodes modified with a thin film consisting of vertically aligned silica mesochannels (SMCs). The perpendicular SMCs allow for the effective mass transport of Ru ( bpy ) 3 2 + and its co-reactants from the solution to the underlying ITO electrode surface. Moreover, due to the ultrasmall diameter (namely 2–3 nm) and the negatively charged surface, SMCs exerted a strong electrostatic attraction to the positively charged Ru ( bpy ) 3 2 + to accelerate its mass transport, resulting in a remarkably increased ECL signal. As a model system, when using tri- n -propylamine (TPrA) as the co-reactant, the intensity of ECL generated at the SMCs-modified ITO electrode was stronger than that at a bare ITO electrode by more than two orders of magnitude (i.e., ∼107-fold increase). In this case, the amount of Ru ( bpy ) 3 2 + required for ECL-based analysis can be considerably reduced. A very low concentration of Ru ( bpy ) 3 2 + , namely 9 μM, was demonstrated to be enough for achieving the sensitive detection of TPrA, nicotine and atropine. Both photomultiplier (PMT) and charge coupled device (CCD) were used to measure the luminescence from the ECL reaction. The obtained detection limits were 0.17 nM for TPrA on the basis of the ECL intensity measurement, 72 nM for nicotine and 38 nM for atropine based on the gray value analysis of captured ECL images.
研究领域:
分析化学
·
电化学
·
生物传感器
·
纳米材料
·
分子探针
·
药物检测
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DOI
高影响因子
危险品化学品经营许可证（带存储）