CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Haoyu Wang,Shiting Chen,Jiqing Liu,Wanyue Liu,Wei Chen,Lin Huang,Chao Yan
In recent years, RPA-CRISPR/Cas12a-based electrochemical biosensors have emerged as highly effective tools for nucleic acid detection. However, the random trans -cleavage characteristics of Cas12a on single-stranded nucleic acid sequences can result in the presence of uncertain nucleic acid residues on the electrode surface post-reaction. This situation complicates subsequent sensitization strategies. In this study, we devised a universal sensitization protocol mediated by terminal deoxynucleotidyl transferase (TdT) , a template-independent DNA polymerase and G-rich spherical nucleic acids (G-rich SNAs) with superior performance. Initially, Cas12a was activated by the specific RPA amplification product and cleaved the closed single stranded DNA (C-ssDNA) on the electrode surface. Then, the TdT enzyme extended residual single stranded DNA (R-ssDNA) on the electrode surface, forming G-Quadruplex structures. Subsequently, G-rich SNAs were immobilized on the electrode surface via π-π stacking interactions with the G-quadruplexes. Consequently, a substantial accumulation of hemin occurred within the G-quadruplex cluster structures, generating significant electrochemical signals. We applied this method to the trace detection of Staphylococcus aureus ( S. aureus ) and achieved a good linear calibration curve with concentrations ranging from 0 CFU/mL to 10 4 CFU/mL. Notably, the limit of detection (LOD) was as low as 1.29 CFU/mL, surpassing that of most similar sensors. These results demonstrate that our proposed universal sensitization method effectively addresses the limitations associated with the random trans -cleavage characteristics of Cas12a.
研究领域:
生物传感
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纳米技术
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分子生物学
·
电化学
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DOI
高影响因子
Nano Energy
·
2024
·
IF 16.8
Junkun Feng,Xiaoyi Liu,Kai Li,Weiwei Zhao,Wenjun Wang,Shaohua Ge,Hong Liu,Jianhua Li
Macrophages are the first line of host defense against bacterial infections and mitochondrial reactive oxygen species (mROS) plays an important role in the bactericidal innate response of macrophages. In this work, we propose the concept of engineering macrophages with artificial mROS generation ability to combat bacterial infection, which is enabled by metal phenolic network-mediated lysosomal escape of piezocatalytic nanoparticles (piezoNPs) and piezotronic effect-enhanced intracellular piezocatalysis. By harvesting ultrasound energy, the engineered macrophages can demonstrate pro-inflammatory phenotype, potent phagocytosis and strong bactericidal activity against Staphylococcus aureus ( S. aureus ). By in situ engineering macrophages at the infection site with targeting ligand-modified piezoNPs, the treatment of S. aureus abscess is further demonstrated. This study reveals the potential of intracellular piezotronic nanocatalysis in enhancing innate immune response of macrophages, providing a non-invasive modulation of macrophages-mediated immunotherapy.
研究领域:
生物医学工程
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免疫学
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纳米技术
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微生物学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
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IF 13.273
Huan Li,Minhui Gong,Jiayu Xiao,Luo Hai,Yuze Luo,Lidan He,Zefeng Wang,Le Deng,Dinggeng He
Combined therapy can improve the curative effect and reduce side effect, so it has a broad application prospect in the treatment of bacterial infections especially bacterial biofilm infections. Herein, we propose a photothermal-activated multifunctional nano-antibacterial platform that is constructed by Introducing indocyanine green (ICG) photosensitizers and silver nanoparticles (AgNPs) onto Molybdenum disulfide (MoS 2 ) nanosheets surface. The constructed nanoplatform (MoS 2 /ICG/Ag) has the combined chemo/photothermal/photodynamic triple-mode antibacterial capability under a single 808 nm laser irradiation. Briefly, the photonic hyperthermia generated by MoS 2 nanosheets can not only directly kill bacteria, but also accelerate the release of ICG and silver ions, and the latter is a commonly used chemical antibacterial reagent. The released ICG can catalyze the conversion of oxygen into singlet oxygen with the help of 808 nm light, achieving the photodynamic sterilization. Encouragingly, the loaded ICG and AgNPs can in turn enhance the heat, which is a mutually reinforcing effect that can produce a combined or even synergistic therapeutic effect. In vitro experiments demonstrate that the MoS 2 /ICG/Ag with triple bactericidal modalities has good broad-spectrum antibacterial performance. Importantly, it can remarkably inhibit the S. aureus biofilms formation and effectively eradicate the bacteria deep in the biofilms. In addition, the MoS 2 /ICG/Ag is successfully used for treating S. aureus biofilms-infected wound in vivo with minimal toxicity. Taken together, the developed MoS 2 /ICG/Ag nanoplatform may serve as a potential nano-antibacterial agent for photothermally activated multimodal combined antibacterial and antibiofilm therapy.
研究领域:
纳米技术
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生物医学工程
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药学
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微生物学
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材料科学
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产品关联
DOI
高影响因子
Food Bioscience
·
2026
·
IF 5.9
Tingting Sun,Lin Jiang,Lijiao Yang,Siyi Chen,Yang Liu,Jing Liu,Fei Xu
Benzo-γ-pyrone derivatives are widely utilized in both the food industry and pharmaceutical research. From a constructed Benzo-γ-pyrone Natural Compounds Database (BNCD), we screened 18 compounds with the core benzo-γ-pyrone skeleton against Staphylococcus aureus α-hemolysin (Hla) and categorized them into five classes (A-E). We identified 10 effective inhibitors by hemolytic assay that could be pooled with the use of a dose-response model to generate an IC 50 curve. Dose-response analysis yielded IC 50 values, which, together with molecular docking results for the Hla secretion regulators (AgrC, AgrA, SarR, SarA), revealed a key pharmacophore. The optimal structure is characterized by a benzo-γ-pyrone backbone featuring a C2-linked B-ring, an essential 5,7-dihydroxy motif on the A-ring, and a B-ring substituted with either a 4′-hydroxyl or a 3′,4′-catechol group. The minimum inhibitory concentrations (MICs) of morin, apigenin, (+/-)-naringenin, and liquiritigenin were all > 2000 μg/mL. Subsequent cytotoxicity assessments (CCK-8 assay) revealed no significant cytotoxicity (P > 0.05) for morin and apigenin up to 2000 μg/mL, for naringenin up to 500 μg/mL, and for liquiritigenin up to 250 μg/mL in bovine mammary epithelial cells. Furthermore, these compounds conferred concentration-dependent protection against S. aureus -induced damage in both in vitro Raw264.7 macrophage and in vivo Galleria mellonella larval infection models. In conclusion, our study identifies specific dietary benzo-γ-pyranones as promising non-antibiotic, anti-virulence agents targeting S. aureus Hla. These findings provide a rational basis for designing functional foods and developing novel anti-virulence therapeutics.
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产品关联
DOI
高影响因子
TALANTA
·
2024
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IF 5.6
Shanshan Lin,Yunsi Zheng,Yanlong Xing,Kun Dou,Rui Wang,Hongwang Cui,Rui Wang,Fabiao Yu
Staphylococcus aureus ( S. aureus ) is the most common pathogen in human purulent infections, which can cause local purulent infections, as well as pneumonia, pseudomembranous enteritis, pericarditis, and even systemic infections. The conventional methods including bacteria colony counting, polymerase chain reaction and enzyme-linked immunosorbent assay can't fully meet the requirement of highly sensitive detection of S. aureus due to their own disadvantages. Therefore, it's an urgent need to develop new platform to detect S. aureus in the early infection stage. In this study, a new surface-enhanced Raman scattering (SERS)-based nanoplatform based on dual-recognition of aptamer (Apt) and vancomycin (Van) was developed for the highly sensitive detection of S. aureus . The SERS nanoplatform consisted of two functional parts: aptamer-conjugated Fe 3 O 4 magnetic nanoparticles (Fe 3 O 4 -Apt MNPs) for bacteria enrichment and vancomycin modified-Au nanoparticles (Van-Au NPs) as the SERS probes for S. aureus quantitative detection. Upon the target bacteria enrichment, the SERS signals of the supernatant after magnetic separation could be obtained and analyzed under different concentrations of S. aureus . The limit of detection of the proposed assay was found to be 3.27 CFU/mL. We believe that the proposed SERS-based nanoplatform has great potential as a powerful tool in the early detection of specific bacteria.
研究领域:
分析化学
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纳米技术
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生物传感器
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微生物学
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临床诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
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2018
·Vol. 20
·期 10
·1-14
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IF 2.127
Hua Jisong,Teng Peng,Zou Yingying,Zhang Chao,Shen Xujie,Cai Jianfeng,Hu Yong
The bacterial infections, especially wound infection, have become a growing problem threatening the human health. Small molecular antimicrobial agents were synthesized to fight against the bacteria but the clinical application in wound inflection treatment was restricted because of their hydrophobicity and poor skin permeation. Here, we synthesized a novel quinoline compound to be used as an antimicrobial agent. Due to its hydrophobic property, this quinoline compound could be successfully encapsulated into poly(epsilon-caprolactone)-poly(ethylene glycol) (PCL-PEG) nanoparticles (NPs) with a mean size of about 100 nm and showed a sustained drug release manner in vitro. Compared to the free drug of this quinoline compound, these drug-loaded PCL-PEG NPs had better water solubility and lower cytotoxicity in vitro. Results of in vivo wound healing experiments demonstrated that these drug-loaded NPs could not only greatly suppress the bacterial infection around the wound, but also significantly accelerate the curing rate of wound. Thus, these quinoline compound-loaded PCL-PEG NPs could act as a promising drug delivery system for wound healing applications.
研究领域:
药剂学
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生物医学工程
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微生物学
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DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2024
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IF 5.4
Lin Wang,Yu Pang,Meihua Xin,Mingchun Li,Lulu Shi,Yangfan Mao
Chitosan exhibits good biocompatibility and some antibacterial activity , making it a popular choice in biomedicine, personal care products, and food packaging. Despite its advantages, the limited antibacterial effectiveness of chitosan hinders its widespread use. Introducing a six-membered heterocyclic structure through chemical modification can significantly enhance its antimicrobial properties and broaden its potential applications. In order to explore the effect of six-membered heterocyclic structure on the antibacterial and antibiofilm activities of chitosan. In this study, seven chitosan derivatives containing six-membered heterocyclics were prepared. They were characterized using Fourier transform infrared (FT-IR) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, and elemental analysis . Cell viability assay showed that they were non-toxic. The antibacterial and antibiofilm activities against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) were evaluated. Our research findings demonstrate that increasing the hydrophobicity , alkalinity and charge density of the substitute groups improved the antibacterial and antibiofilm activities of chitosan. This study also offers valuable insights into the quantitative structure-activity relationships of chitosan derivatives in terms of antibacterial and antibiofilm activities.
研究领域:
生物医学
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材料科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
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2019
·Vol. 17
·期 1
·1-14
·
IF 5.345
Gasmalla Hind Baballa,Lu Xiaoquan,Shinger Mahgoub Ibrahim,Ni Lubin,Chishti Aadil Nabi,Diao Guowang
Background Iron oxide nanocomposites have received a great attention for their application in various fields like physics, medicine, biology, and material science etc., due to their unique properties, such as magnetism, electrical properties, small size, biocompatibility and low toxicity. Methods Fe 3 O 4 /Ag 3 PO 4 @WO 3 nanocomposites with different weight percent of Ag 3 PO 4 were successfully prepared through fabricated Ag 3 PO 4 /Fe 3 O 4 with WO 3 via in situ fabrication method, electrospinning involved precursor solution preparation and spinning to enhance photocatalyst performance under simulated sunlight for the degradation of methylene blue (MB) and antibacterial activity against Staphylococcus aureus ( S. aureus ). Results The photocatalytic degradation of methylene blue (MB) under simulated light irradiation indicated that the nanocomposite with 0.25 mg of Ag 3 PO 4 has the best activity. An additional advantage of these photocatalysts is magnetic recoverability, using external magnetic field and photocatalytic stability of the nanocomposites was evaluated for three cycles. In addition, using different scavengers, holes (h + ) and superoxide radical (O
·−) 2
radicals and hydroxide radical ( · OH) were identified the main oxidative species in the degradation reaction of methylene blue. Conclusions The results reveal that Fe 3 O 4 /Ag 3 PO 4 @WO 3 -0.25 nanocomposites have photocatalytic and antibacterial activity against S. aureus. The photocatalyst and mechanism based on the enhancement of electron transfer processes between Ag 3 PO 4 and WO 3 nanoparticles.
研究领域:
材料科学
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环境科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Shixin Liu,Xiang Cai,Wei Xue,Dong Ma,Wu Zhang
We developed a co-delivery system of nitric oxide (NO) and antibiotic for the antibiotic-resistant bacterial infection therapy. The NO could disperse the bacterial biofilms and convert the bacteria into an antibiotic-susceptible planktonic form. Using the chitosan-graft-poly(amidoamine) dendrimer (CS-PAMAM) as the co-delivery system, methicillin (MET) and NO were conjugated successively to form CS-PAMAM-MET/NONOate. The positive CS-PAMAM could efficiently capture the negatively charged bacteria and PAMAM provide abundant reaction points for high payloads of NO and MET. The CS-PAMAM-MET/NONOate displayed effective and combined antibacterial activity to the E. coli and S. aureus . Particularly, for the MET-resistant S. aureus (MRSA), the CS-PAMAM-MET/NONOate displayed the synergistic antibacterial activity. In vivo wound healing assays also confirmed that CS-PAMAM-MET/NONOate could heal the infection formed by MRSA and then accelerate the wound healing effectively. Moreover, CS-PAMAM-MET/NONOate showed no toxicity towards 3T3 cells in vitro and rats in vivo , providing a readily but high-efficient strategy to drug-resistant bacterial infection therapy.
研究领域:
药剂学
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生物医学工程
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微生物学
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Guotai Yang,Xiangyu Meng,Yutong Wang,Mingyao Yan,Zoraida P. Aguilar,Hengyi Xu
A novel 2-step lectin-magnetic separation (LMS) method combined with gold nanoparticle (AuNPs) -based colorimetric system for specifically and quantitatively detecting S. aureus in blood samples was established. In this study, wheat germ agglutinin lectin from Triticum vulgaris (wheat) (WGA) was applied for the first time as a recognition agent in a 2-step magnetic separation method using magnetic nanoparticles (MNPs) for effective and low-cost bacteria enrichment. A quantitative detection strategy based on the long dsDNA amplicons stabilized with AuNPs against salt-induced aggregation was investigated. The 2-step LMS method resulted in a high enrichment efficiency even at low concentrations (10 0 –10 2 CFU/mL) of S. aureus in pure culture and blood samples. The AuNPs-based colorimetric system was applied for quantitative detection of S. aureus with a limit of detection (LOD) at 3.5 × 10 0 CFU/mL. The complete process starting with the blood samples treatment, 2-step LMS, and AuNPs-based colorimetric detection took only 7 h. The low cost and sensitive 2-step LMS combined with AuNPs-based colorimetric detection holds considerable potential for early sepsis diagnosis.
研究领域:
生物技术
·
纳米技术
·
微生物学
·
临床诊断
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Anni Cui,Yiting Hou,Jialu Zhang,Xin Mu,Huan Wang,Yu Sun,Haitao Xu,Guiye Shan
Staphylococcus aureus (S. aureus) infections pose a serious threat to human health worldwide. Therefore, selective and accurate detection of S. aureus is necessary for preventing infections. Herein, we design the colorimetric-electrochemical sensing platform based on specific oligonucleotide (SA31 aptamer)-tunable oxidase-like activity of mesoporous polydopamine/MnO 2 (MPDA/MnO 2 ) nanozymes for the S. aureus detection. The MPDA/MnO 2 nanozymes possess excellent oxidase-like activity and electrochemical property. MPDA/MnO 2 nanozymes can catalyze the oxidation of TMB to generate TMB + (blue color) and TMB 2+ (yellow color) products. The oxidase-like activity of MPDA/MnO 2 can be inhibited when the SA31 is adsorbed on the surface of the nanozymes. In the presence of S. aureus, SA31 aptamer desorbs from MPDA/MnO 2 surface based on the specific identification between S. aureus and aptamer, resulting in the recovery of their oxidase-like activity. Based on the different oxidative degree of TMB, the color of sensing solution changes from blue to yellow, the electrochemical signals also have changed simultaneously. The colorimetric-electrochemical sensing platform can accurately detect S. aureus, showing a low detection limit in 3 CFU/mL (S/N = 3). Furthermore, the dual-mode sensing platform can be utilized for the accurate and specific detection of S. aureus in real sample with the recovery of 95.15%− 115.28%. This work based on SA31 aptamer-tunable oxidase-like activity of MPDA/MnO 2 nanozymes provides a dual-mode assay for specific and accurate detection of S. aureus.
研究领域:
生物化学
·
分析化学
·
医学诊断
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产品关联
DOI
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2025
·
IF 4.9
Xinxin Liu,Zhengfei Hong,Rongrong Li,Shuxian Hou,Xuechao Shi,Guoqiang Yang,Yuxuan Wang,Yimei Wang,Zhiru Tao,Jun Ge,Fei Ge,Yiyan Song,Jun Wang
Wound infections caused by surgery, chronic ulcers, or trauma have been a challenge for clinicians, especially multiple resistant bacteria induced by the misuse of antibiotics, which has increased the risk of bacterial infection. It is very urgent to design and develop antimicrobial materials with potential for clinical application against drug-resistant bacterial infections. In this study, a photothermal montmorillonite-chitosan hydrogel system (MNT-CS Gel) was fabricated as a photothermal antimicrobial dressing. This study found for the first time that montmorillonite (MNT) has good photothermal properties and photothermal conversion efficiency under near-infrared (NIR) light, and on this basis, its application research was carried out. The MNT possesses broad-spectrum antibacterial activity with significant inhibitory effects against E. coli and S. aureus, excellent photothermal stability under NIR light. And all the performances were improved after combining with CS-Gel, especially in bacterial adsorption capacity and photothermal performance. Notably, MNT-CS Gel was used as a photothermal antibacterial agent for the first time, and the antibacterial effects in vitro and their mechanisms have been systematically investigated. It was found that the MNT-CS Gel had significant inhibitory and destructive effects on bacteria in an in vitro pigskin model. The MNT-CS Gel could adsorb the bacteria strongly, inhibit bacterial biofilm formation, induced the generation of ROS, destroy the structure of bacteria, and cause the leakage of nucleic acids. The MNT-CS Gel demonstrated good photothermal antimicrobial properties, suggesting that the MNT-CS Gel has potential for clinical hygiene applications.
研究领域:
生物医学工程
·
材料科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）