ENVIRONMENTAL POLLUTION
·
2024
·
IF 7.6
Shuyang Huang,Zhijia Zhang,Cong Li,Yasong Luo,Guoxia Zhang
Diethyl ethylphosphonate (DEEP) as a novel organophosphorus flame retardant received increasing attention and its structure was discovered. But there are currently insufficient studies on how DEEP exposure affects the gut microbiome. In this study, the effects of DEEP on the structure and function of the human gut microbiota were examined using the SHIME system. Results from high-throughput sequencing of the 16S rRNA gene show that the high dose DEEP exposure reduced the Shannon and Simpson index in the transverse and descending colon. The Bacillota had the highest proportion while the proportion of Proteobacteria gradually decreased at the phylum level. The abundance of Escherichia , Prevotella , and Bilophila at the genus level increased with increasing doses of DEEP exposure. On the contrary, the abundance of Megasphaera , Klebsiella , and Phascolarctobacterium decreased. The short-chain fatty acids had a significant shift. With increasing doses of DEEP exposure, the concentration of acetic acid and propionic acid increased, while the concentration of butyric acid reached the highest at the medium dose of exposure. In addition, Bilophila , Psychrobacter , Escherichia , and Nostoe showed strong beneficial associations with acetic and propionic acids under DEEP exposure. Phocaeicola , Agathobacter , Klebsiella , Megasphaera , Phascolarctobacterium, and Bacteroides were negatively association with acetic and propionic acids. In a word, the study verified that exposure to different doses of DEEP can cause changes in the composition of the gut microbiome and metabolite SCFAs, which provides ideas for the investigation of other potential hazards of DEEP on human beings.
研究领域:
微生物学
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毒理学
·
环境健康
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DOI
PubMed
高影响因子
Food Frontiers
·
2025
·
IF 6.9
Yanqi Peng,Ji Wu,Ronghua Fan,Mingyue Ma,Xixin Wang,Yuzhen Pi,Xiqing Yue,Yanyu Peng
The development of new therapeutic strategies for ulcerative colitis (UC) requires the targeting of multiple pathogenic factors, including disruption of the colonic mucosal barrier, dysregulated inflammation, and gut microbiota imbalance. This study aimed to investigate the protective mechanisms of almond polysaccharide (AP1) against dextran sulfate sodium (DSS)-induced UC in mice. Administration of AP1 significantly restored DSS-induced reductions in goblet cells and inhibited epithelial apoptosis, thereby preserving colonic mucosal barrier integrity. In addition, AP1 decreased the levels of proinflammatory cytokines and oxidative stress markers in colonic tissues. Gut microbiota analysis revealed that AP1 effectively restored microbial composition and diversity. Furthermore, AP1 increased the relative abundance of beneficial bacteria while reducing pathogenic taxa, thereby contributing to the restoration of intestinal microbial balance in UC mice. Metabolomic analysis of short-chain fatty acids (SCFAs) demonstrated that DSS-induced UC significantly depleted major SCFAs (acetic acid, propionic acid, n-butyric acid) and disrupted branched-chain fatty acid (i-butyric acid and i-valeric acid) metabolism, whereas AP1 intervention reestablished SCFA profiles. Correlation analysis showed Bacteroides negatively correlated with multiple SCFAs, whereas Alistipes positively correlated with propionic acid. Collectively, these findings underscore the role of AP1 in rescuing microbiota-derived SCFA metabolism. In summary, AP1 was shown to alleviate UC symptoms through multiple mechanisms, including the preservation of mucosal barrier integrity, suppression of oxidative stress and inflammation, and modulation of gut microbiota composition and the intestinal microenvironment. These results support the therapeutic potential of AP1 as a natural polysaccharide-based agent for UC treatment.
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Yong-Gang Xia,Tian-Long Wang,Si-Miao Yu,Jun Liang,Hai-Xue Kuang
The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GC MS based monosaccharide compositions, Smith degradations, and methylation analysis. The result showed average molecular weights of AERP were between 42.7 kDa and 93.9 kDa. AERP was composed of Ara, Rha, GlcA, Man, Glc, and Gal in a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2 along with a small number of sulfate (3.38%) and acetyl (4.87%) groups. The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected. Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays. GC MS was further used to determine the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice. These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC–MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.
研究领域:
药理学
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生物化学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Xinmiao Wang,Qian Wang,Dong Cai,Xu Guo,Peiyong Tong,Fawen Yin,Xiaoyang Liu,Dayong Zhou
Phenolipids such as hydroxytyrosol-SCFA acyl esters (HTy-SEs) and tyrosol-SCFA acyl esters (TYr-SEs) with various alkyl chains lengths (C1–C4) and different isomers (branched-chain and straight-chain) were successfully synthesized. All esters were hydrolyzed by pancreatic lipase to produce polyphenols (HTy and TYr) and SCFAs (iso-butyric acid, acetic acid, propionic acid, and n-butyric acid). Moreover, HTy-SEs (and TYr-SEs) could also be hydrolyzed to free HTy (and TYr) and SCFAs by gut microbiota and Lactobacillus from mice feces. Especially, the hydrolysis rates showed positive correlation with the carbon skeleton length, and the hydrolysis degree (DH) of ester with a branched-chain fatty acid was weaker than that of ester with a straight-chain fatty acid. Besides, the DH values of TYr -SEs were significantly higher than those of HTy-SEs. Therefore, through regulating the structures of polyphenols, carbon skeleton lengths, and isomers, controlled-release of polyphenols and SCFAs from phenolipids will be easily achieved.
研究领域:
食品科学
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营养学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2023
·
IF 5.6
Yingyu Zhang,Yuan Sun,Yupei Liu,Jiameng Liu,Jing Sun,Yajuan Bai,Bei Fan,Cong Lu,Fengzhong Wang
Polygonum sibiricum polysaccharides (PSP) are one of the main active components of Polygonatum sibiricum . The present study was performed to deeply explore the antidepressant-like effects of PSP on the chronic unpredictable mild stress (CUMS)-induced depression and underlying mechanisms. PSP markedly reversed the depression-like behavior and significantly increased the levels of 5-hydroxytryptamine and norepinephrine, decreased the levels of the pro-inflammatory cytokines in the hippocampus and the corticosterone and adrenocorticotropic-hormone in the serum of CUMS-induced mice. Gut microbiota dysbiosis focusing on Bacteroidetes and Firmicutes and changes of SCFAs especially for butyrate, propionate and acetate were remarkably reversed by PSP. These findings indicated that the antidepressant-like effects of PSP were related to inhibit the HPA axis hyperactivation, normalize the neurotransmitter levels, suppress the neuroinflammation, regulate the composition of gut microbiota and the levels of SCFAs. The current study provides a new insight into the potential of PSP in prevention and treatment of depression.
研究领域:
药理学
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神经科学
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微生物学
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精神病学
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产品关联
DOI
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2019
·Vol. 63
·期 14
·1801363
·
IF 4.653
Shanshan Zhang,Haijuan Hu,Wanying He,Zafarullah Muhammad,Lufeng Wang,Fengxia Liu,Siyi Pan
Scope The prebiotic regulation of the gut microbiota is a promising strategy to induce protective humoral and mucosal immune responses. The potential immune-improving effects of pectin oligosaccharides (POS) in healthy mice and the potential mechanism mediated by specific intestinal bacteria are investigated. Methods and results POS is prepared using a hydrogen-peroxide-assisted degradation. Mice that consumed diets containing POS are tested for microbial community shifts, short-chain fatty acids (SCFAs), and immunoglobulin (Ig) production using quantitative real-time polymerase chain reaction, gas chromatography, and ELISA kits. Pearson's correlation analyses are performed between Ig production and specific intestinal bacteria or SCFAs. POS treatment significantly improves the growth of healthy mice. Moreover, 4-week POS administration results in a profound change in intestinal microbial composition and a significantly higher fecal concentration of acetate, which leads to substantial increases of the levels of fecal secretory immunoglobulin A and serum IgG. Conclusions The results suggest that the inclusion of POS in a diet can increase Ig production and optimize the composition of the gut microbiota. A significant correlation is observed between changes in Ig production and specific intestinal bacteria or acetate, providing insight into the mechanism of POS as a potential immune-enhancing supplement.
研究领域:
免疫学
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微生物学
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营养学
·
益生元
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产品关联
DOI
PubMed
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2020
·Vol. 64
·期 8
·1901251
·
IF 5.309
Lingjun Tong,Haining Hao,Xinyi Zhang,Zhe Zhang,Youyou Lv,Lanwei Zhang,Huaxi Yi
Scope Milk-derived extracellular vesicles (mEVs) as nanoparticles are being developed as novel drug vehicles due to their pivotal role in cell–cell communication. As an important bioactive component in milk, little is known about their effect on the gut microbiota and intestinal immunity. Therefore, the effects of mEVs on gut microbiota and intestinal immunity in mice are investigated. Methods and results First, a new method to obtain high-yield mEVs is developed. Afterward, the colonic contents from C57BL/6 mice fed different doses of mEVs (8 weeks) are collected and the microbial composition via 16S rRNA gene sequencing is analyzed. It is found that mEVs could alter the gut microbiota composition and modulate their metabolites—short-chain fatty acids (SCFAs). Furthermore, the effects of mEVs on intestinal immunity are evaluated. It is observed that the expression levels of Muc2, RegIIIγ, Myd88, GATA4 genes, and IgA, sIgA are increased in the intestine, which are significant for the integrity of the mucus layer. Conclusion These findings reveal that the genes with critical importance for intestinal barrier function and immune regulation are modified in mice by oral administration mEVs, which also result in the changes of the relative composition of gut microbiome and SCFAs.
研究领域:
药理学
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微生物学
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免疫学
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营养科学
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DOI
PubMed
高影响因子
Environment & Health
·
2026
·
IF 8.8
Yun Hong,Yujie Qiu,Qingqing Yang,Bingbing Sun,Yuheng Zhang,Zhiyuan Zhu,Zuo Li,Tong Shen
Bisphenol A (BPA), frequently detected at low levels in pregnant women, has been linked to long-term metabolic disturbances in offspring. Emerging evidence indicates that maternal gut microbiota critically influence offspring susceptibility to metabolic diseases through their metabolites. This study aimed to investigate how maternal gut microbiota and short-chain fatty acids (SCFAs) mediate the increased susceptibility to high-fat-diet (HFD)-induced metabolic disorders in adult offspring following gestational low-dose BPA exposure. Pregnant mice were exposed to 0, 10, 100, and 1000 nmol/L BPA via drinking water throughout gestation, after which pups were cross-fostered by unexposed dams and fed normal chow or HFD until adulthood. Adult offspring from BPA-exposed dams exhibited aggravated HFD-induced dyslipidemia and insulin resistance. Maternal BPA exposure decreased the abundance of f_Prevotellaceae and g_Bacteroides, accompanied by reduced circulating SCFAs in both dams and offspring. Reduced SCFAs impaired embryonic pancreas and intestinal development by downregulating GPR41 and GPR43, which may predispose offspring to metabolic disorders at adulthood. Importantly, maternal high-fiber diet restored gut microbiota composition along with SCFA production, normalized embryonic GPRs expression, and subsequently alleviated offspring metabolic impairments. These findings provide key insights into BPA-induced developmental programming of metabolic disorders and offer promising strategies against metabolic dysfunction induced by endocrine-disrupting chemicals.
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DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Shanshan Zhang,Yonggan Sun,Qixing Nie,Jielun Hu,Yuhao Li,Zefu Shi,Haihua Ji,Hao Zhang,Mingjiao Zhao,Chunhua Chen,Shaoping Nie
Hydrocolloids are important food additives and have potential regulatory effects on gut microbiota. The development of colitis is closely related to changes in gut microbiota. Food hydrocolloids have potential microbial-modulating effects, but their impact on colitis is not well studied. Therefore, this study investigated the effects of four hydrocolloids (carrageenan, guar gum, xanthan gum, and pectin) on colitis, and explored their regulatory effects on gut microbiota. The results indicated that pectin and guar effectively alleviated body weight loss and disease activity index, reduced inflammatory cytokine levels, and promoted short-chain fatty acids (SCFAs) production. They increased the abundance of Akkermansia muciniphila , Oscillospira , and Lactobacillus , and Akkermansia abundance had a negative correlation with the severity of colitis. In contrast, carrageenan and xanthan gum did not significantly improve colitis, and carrageenan reduced the production of SCFAs. Both carrageenan and xanthan gum increased the abundance of Ruminococcus gnavus , and Ruminococcus abundance was positively correlated with the severity of colitis. These findings suggest that food additives have an impact on host health and provide guidance for the diet of patients with colitis.
研究领域:
食品科学
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胃肠病学
·
微生物学
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Denglong Lu,Yang Song,Huanying Ge,Hongjia Peng,Haipu Li
Large amounts of waste activated sludge are generated daily worldwide, posing significant environmental challenges. Anaerobic fermentation is a promising method for sludge disposal, but it has two technical bottlenecks: the availability of short-chain fatty acids (SCFAs)-producing substrates and SCFAs consumption by methanogenesis. This study proposes a pretreatment strategy combining sodium percarbonate (SPC) and magnetite (Fe 3 O 4 ) to address these issues. Under optimized conditions (20 mg Fe 3 O 4 /g TSS and 15 mg SPC/g TSS), SCFAs production increased to 3244.10 ± 216.31 mg COD/L, about 3.06 times the control (1057.29 ± 35.06 mg COD/L) and surpassing reported treatments. The combined pretreatment enhanced the disruption of extracellular polymeric substances, increased the release of biodegradable matters, improved acidogenesis enzyme activities, and inhibited methanogenesis. Additionally, it increased NH 4 + -N release in favor of the recovery of phosphorus from sludge residual. This study demonstrates an efficient pretreatment for high SCFAs production and resource recovery from WAS.
研究领域:
环境工程
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生物技术
·
废物管理
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Jinping Zhou,Laifeng Chen,Hooi Ling Foo,Zhenhui Cao,Qiuye Lin
The present study aimed to determine microbial community , short-chain fatty acids (SCFAs), and volatilome of Bulang pickled tea during fermentation. Sequencing of 16S rRNA and ITS revealed that Bualng pickled tea was dominated by Lactobacillus plantarum , unclassified Enterobacteriaceae , unclassified Debaryomyces , Candida metapsilosis, Cladosporium sphaerospermum , and unclassified Aspergillus . The overall contents of SCFAs increased, with acetic acid showing the highest content. A total of 398 differential volatile metabolites were detected using differential metabolomics analysis. Out of these different volatile compounds , ten key volatile compounds including (Z)-4-heptenal, 1-(2-thienyl)-ethanone, 5-methyl-(E)-2-hepten-4-one, 2-ethoxy-3-methylpyrazine, p-cresol, 2-methoxy-phenol, ethy-4-methylvalerate, 3-ethyl-phenol, p-menthene-8-thiol, and 2-s-butyl-3-methoxypyrazinewere were screened based on odor activity value (OAV). The Spearman correlation analysis showed a high correlation of SCFAs and volatile compounds with microorganisms, especially L. plantarum and C. sphaerospermum . This study provided a theoretical basis for elucidating the flavor quality formation mechanism of Bulang pickled tea.
研究领域:
食品科学
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微生物学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Xinmiao Wang,Qian Wang,Yuanyuan Hu,Fawen Yin,Xiaoyang Liu,Dayong Zhou
The models of rat everted gut sac and hydrolysis by rat plasma were used to clarify the hydrolysis and transport characteristics of tyrosol-SCFA esters (TYr-SEs). HPLC-UV results indicated that TYr-SEs could be hydrolyzed by intestinal lipase, which showed sustained release of SCFAs and TYr. Meanwhile, TYr-SEs and the liberated SCFAs and TYr could cross the membrane and were transported into blood circulation. TYr-SEs were further hydrolyzed by carboxylesterase in plasma. Obviously, the hydrolysis of TYr-SEs in blood also showed sustained release of SCFAs and TYr. Especially, the rates of hydrolysis and transport correlated positively with the acyl chain lengths. Besides, the above rates of the TYr-SE with a straight chain were greater than those of its isomer with a branched chain. Therefore, the above-mentioned two-step release of SCFAs and TYr clearly demonstrated that TYr-SEs would be an effective approach to enhance the beneficial health effects of SCFAs and TYr.
研究领域:
营养学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）