FUEL
·
2024
·
IF 6.7
Chang Li,Kang Tan,Fangzheng Hua,Changxue Wang,Xiaolin Wang,Fei Wang,Mengting Sun
Hydrate-based natural gas storage and transportation is in urgent need of efficient and eco-friendly promotion methods. Here, we employed a kind of eco-friendly hydrogels, which were polyvinyl alcohol-co-acrylic acid (PVA-co-PAA), as carriers for trace SDS solution to construct a co-promoter for methane hydrate formation. Under static condition without stirring, trace SDS solution that was lower than or equal to 0.4 mmol L −1 had no or had extremely weak promoting effects on hydrate formation kinetics, whereas hydrogel-carried trace SDS exhibited excellent synergistic promoting effects. The HS-0.4 under a SDS concentration of 0.4 mmol L −1 and at a swelling ratio of 200 g g −1 achieved the most remarkable promotion, and the induction time was 0 min, the hydrate growth rate at t 90 was 0.1552 ± 0.0068 mmol mL −1 min −1 , the methane uptake was 121.70 ± 1.58 mmol mol −1 and the water-to-hydrate conversion was 0.730 ± 0.009. During synergistic promotion process, on the one hand, hydrogels keeping with large surface area increased gas–liquid interface, and were in charge of facilitating hydrate nucleation and shortening induction time; on the other hand, trace SDS produced strong capillary effect and sharply boosted mass transfer during hydrate growth period, which drastically enhanced hydrate growth rate and methane uptake. Morphology observation on hydrate evolutions unveiled that the co-promoter significantly improved poor hydrate compactness and partly relieved foam problem caused by SDS. Hydrogel-carried trace SDS can serve as an effective, eco-friendly and economical promotion approach for methane hydrate formation, which might be of great benefit for hydrate-based natural gas storage and transportation.
研究领域:
化学工程
·
材料科学
·
环境科学
·
能源存储与运输
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2013
·Vol. 36
·期 15
·2530-2537
·
IF 2.591
Maria del Mar Barrios-Romero,Agustín G. Crevillén,José Carlos Diez-Masa
This work describes the development of an SDS-gel electrophoresis method for the analysis of major whey proteins (α-lactalbumin, β-lactoglobulin, and BSA) carried out in SU-8 microchips. The method uses a low-viscosity solution of dextran as a sieving polymer. A commercial coating agent (EOTrol LN) was added to the separation buffer to control the EOF of the chips. The potential of this coating agent to prevent protein adsorption on the walls of the SU-8 channels was also evaluated. Additionally, the fluorescence background of the SU-8 material was studied to improve the sensitivity of the method. By selecting an excitation wavelength of 532 nm at which the background fluorescence remains low and by replacing the mercury arc lamp by a laser in the detection system, an LOD in the nanomolar range was achieved for proteins derivatized with the fluorogenic reagent Chromeo P540. Finally, the method was applied to the analysis of milk samples, demonstrating the potential of SU-8 microchips for the analysis of proteins in complex food samples.
研究领域:
分析化学
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蛋白质组学
·
食品分析
·
微流控技术
✓ 已验证
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2020
·
IF 2.952
Han Huang,Yun Lin,Guozeng Wang,Juan Lin
A new pullulanase-encoding gene, pulSL3 , was cloned from Alkalibacterium sp. SL3 and expressed in Escherichia coli . Deduced PulSL3 contains 2026 amino acid residues and consists of a putative signal peptide, four carbohydrate-binding modules, an E_set pullulanase domain, an α-amylase domain, and a pullulanase domain. The purified rPulSL3 exhibited pullulanase activity only. To identify the functions of the α-amylase and pullulanase domains, the N- and C-terminus truncated mutants ( pulSL3△N and pulSL3△C ) were also expressed in E. coli. The purified rPulSL3△N had pullulanase activity, while rPulSL3△C had no either α-amylase or pullulanase activity. Both rPulSL3 and rPulSL3△N exhibited maximum activities at pH 9.0 and 50 °C, and were highly active and stable at the concentration of NaCl up to 4 M and resistant to a few surfactants and detergents. In comparison to the wild type, rPulSL3△N had better thermostability (retaining 62.9 vs. 33.6% activity at 52 °C for 30 min), higher substrate affinity (with K m of 0.10 vs. 0.15 mg mL −1 ) and higher catalytic efficiency (836.6 vs. 520.3 mL mg −1 s −1 ). These excellent properties make rPulSL3△N potential for the application in the industries of detergent, food, and environmental remediation.
研究领域:
微生物学
·
酶学
·
生物技术
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产品关联
DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Shuo Li,Sheng Lei,Qian Yu,Lina Zou,Baoxian Ye
A novel high-sensitive electrochemical sensor with glassy carbon electrode (GCE) as support for hyperin determination is successfully designed and constructed, and the well-shaped nano-meter modified material is synthesized via a one-step and facile route. Functionalized with surfactant sodium dodecyl sulfate (SDS), Single-Walled Carbon Nanotubes (SWCNTs) are synchronously grafted with ZrO 2 nanoparticles to develop into the as-prepared nano-composite (ZrO 2 -SDS-SWCNTs). Compared to the previous reports related with hyperin detection, the linear range gets wider and detection limit (LOD) becomes lower with the aid of this novel nano-composite modified glassy carbon electrode (ZrO 2 -SDS-SWCNTs/GCE). The crystalline phases and functionalization of the preparation process has been investigated by X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) instrument analysis, respectively, and the micro-morphology of related modified materials is also visibly characterized by scanning electron microscope (SEM) and transmission electron microscope (TEM). In addition, electrochemical properties of the modified materials are comparably explored by means of impedance spectroscopy (EIS) and cyclic voltammograms (CV). According to the established calibration curve under optimized condition, the peak current (Differential pulse voltammetry (DPV) signal) keeps a linear relationship with hyperin concentration in the ranges of 1.0 × 10 −9 − 3.0 × 10 −7 mol L −1 , meanwhile detection limit reaches as low as 5 × 10 −10 mol L −1 (S/N = 3). As for practical applications, the proposed sensor has also worked well on sensitive hyperin determination in real species Abelmoschus manihot.
研究领域:
分析化学
·
纳米技术
·
电化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 28
·期 26
·35113-35125
·
IF 4.223
An Shaorong,Jin Qiang
In recent years, the excess discharge of ammonia nitrogen from wastewater into surface water has been regulated by more stringent standard. The air stripping method is successfully used to treatment of high-concentration ammonia nitrogen; however, alkali will be added to keep pH more than 10, which is costly and not environment-friendly operation. In this study, an advanced air stripping (AAS) based on foam separation of removing ammonia nitrogen in low concentration from aqueous solution at low pH was proposed. The effect of conditions such as air flow rate, temperature, SDS dosage, coexisting ionic strength, pH, and initial ammonia nitrogen concentration on the removal efficiency was studied. The advanced air stripping exhibited favorable removal efficiency for NH 4 + -N in low concentration from aqueous solution (20 mg·L −1 ) with a broad range of low pH 3.0–9.0. Besides, for strongly alkaline (pH=11.0) solution, the advanced air stripping can alleviate the decrease of pH to some extent and keep ammonia nitrogen stripping out continuously based on equilibrium shift between NH 4 + and NH 3 . A microcalorimeter was applied to demonstrate the interaction between the negatively charged hydrophilic groups of SDS and NH 4 + ions, helping to understand the mechanisms more clearly. The simple operation and the satisfactory removal efficiency could imply that the advanced air stripping is a promising technology for minimizing low-concentration NH 4 + -N. Graphical abstract
研究领域:
环境科学
·
废水处理
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2021
·
IF 3.512
Qiming Zhuo,Wenli Liu,Hongxiang Xu,He Zhang,Xiaopeng Sun
Attachment behavior is a key component of flotation and has a decisive influence on flotation performance, and the experiment research on the attachment between mineral particles and bubbles still needs further research. In this work, a particle–bubble attachment apparatus and multiple target tracking software were developed. Coal particles were used as the subjects, and the effect of particle properties on the attachment performance was studied from the perspective of the particle group. The particle–bubble attachment experiments indicated that the collision position had an effect on the attachment efficiency, and the attachment efficiency decreased with an increase in the collision angle. The efficiency-weighted attachment angle was proposed to quantitatively describe the attachment performance of coal samples. The efficiency-weighted attachment angle of low-density coal samples was greater than that of high-density coal samples. For particles with different sizes, the efficiency-weighted attachment angle of fine particles was greater than that of coarse particles. Furthermore, SDS weakened the attachment performance between coal particles and bubbles via adsorption on the bubble, and the efficiency-weighted attachment angle decreased as the concentration of the SDS solution increased. CTAB adsorbed on coal particles and bubbles, and the efficiency-weighted attachment angle first increased and then decreased with increasing CTAB concentration.
研究领域:
矿物加工工程
·
表面化学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING RESEARCH & DESIGN
·
2024
·
IF 3.7
Yulong Jiang,Danhua Xie,Xiuping Hu,Nan Ye,Siying Chen,Yingying Li,Biao Ma
Solar evaporation systems represent a green and sustainable strategy for obtaining fresh water. However, traditional evaporation processes depend on the heating of bulk water. Recently, floating solar absorbers have demonstrated excellent evaporation efficiency via localized heating at the device–water interface. In this work, a floating carbon-based hybrid porous evaporator was prepared through simple and inexpensive phase conversion methods, effectively improving the water evaporation efficiency two-fold compared to pure water. The evaporators and evaporation process were characterized by mercury porosimeter, scanning electron microscope , near-infrared spectrometer, infrared thermography , and differential scanning calorimetry . Furthermore, by introducing sodium dodecyl sulfate to regulate the surface hydrophobicity and hydrogen bond structure of water at the device–water interface, the evaporation rate was further improved to 1.27 kg·m −2 ·h −1 (three times that of pure water) at the optimal sodium dodecyl sulfate concentration of 0.3–1 mmol·L −1 . The system also demonstrated excellent practical performance and durability in the evaporation of seawater , salt solutions, and under natural sunlight conditions. This work creates a new avenue for the evaporation applications of hydrophobic carbon-based materials and provides a strategy to regulate solar evaporation systems from the perspective of bulk water by introduction of a surfactant .
研究领域:
材料科学
·
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
ENERGY & FUELS
·
2022
·
IF 4.654
Wenlian Rong,Yu Zhang,Shengjun Luo,Rongbo Guo
To speed up the formation of methane hydrate, copper nanowires (CuNWs) doped with sodium dodecyl sulfate (SDS) were used as a promotor. Transmission electron microscopy (TEM) images and elemental analysis of copper, oxygen, and sulfur indicated that SDS was successfully doped on the surface of copper nanowires. Compared with the SDS solution, the average hydrate induction time required for CuNW-SDS was significantly shortened from 71.25 ± 22.41 to 12 ± 4.89 min due to the strong thermal conductivity of copper nanowires. When SDS was used, the hydrate growth rates were about 0.56 ± 0.25 mmol/min. For CuNW-SDS, the hydrate growth rate was increased to 1.04 ± 0.53 mmol/min, which indicated that CuNW-SDS with better dispersibility enhanced the hydrate growth rate over 86% compared to that of SDS. In addition, when CuNW-SDS was used as a promoter, there were more dense hydrates, fewer foams generated by dissociation, and excellent recycling performance in the process of methane hydrate formation and dissociation.
研究领域:
化学工程
·
材料科学
·
能源科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF POROUS MATERIALS
·
2019
·Vol. 27
·期 1
·29-35
·
IF 1.947
Li Jie,zhang Jia,Chen Lianxi,Wen Zhuoqiong,Luo Jie,Yu Cui,Wang Pengpeng
In this study, vinyl-functionalized porous silica (V-P-SiO 2 ) and ureido-functionalized porous silica (UD-P-SiO 2 ) have been synthesized separately by using hexadecyl trimethyl ammonium bromide (CTAB) and sodium dodecyl sulfonate (SDS) as mixed templates. The samples were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectrum (FTIR) and N 2 adsorption–desorption measurement. The results showed that UD-P-SiO 2 with a diversity of morphologies were obtained by judiciously chosen mole ratio of SDS to CTAB (R = n SDS :n CTAB ). On the contrast, the mole ratio of surfactants had less effect on the morphologies of V-P-SiO 2 until the mole ratio of SDS to CTAB was reached 1.6, however, FTIR proved that it had effect on the vinyl groups in the V-P-SiO 2 network framework.
研究领域:
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
PHARMACEUTICAL RESEARCH
·
2020
·Vol. 37
·期 11
·1-12
·
IF 3.242
Shen Bin-Bin,Zhang Zhongwei,Yuan Jun-Jie,Zheng Aiping,Zeng Su,Gao Jian-Qing,Bao Wenhan,Barnard James,Wang Haibin,Fang Wei-Jie
Purposes The main purposes of this article are to describe an unprecedented phenomenon in which significant amount of a shoulder peak impurity was observed during normal non-reducing capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) analysis of a recombinant fusion protein X, and to evaluate the root cause for this phenomenon. Methods A series of experiments were conducted to study the nature of this degradation. Effects of iodoacetamide (IAM), heating temperature, duration, and SDS on the formation of this specific impurity were evaluated using a variety of characterization techniques. Results The formation of the impurity as observed in CE-SDS was actually due to alkylation of lysine and serine residues with IAM, as confirmed by peptide mapping and LC-MS/MS, which increased the molecular weight and therefore decreased the electrophoretic mobility. The amount of impurity was also strongly dependent on sample preparation conditions including the presence or absence of SDS. Conclusions Our study clearly suggested that even though IAM has been used extensively as an alkylation reagent in the traditional non-reducing CE-SDS analysis of monoclonal antibodies and other proteins, alkylation with IAM could potentially lead to additional impurity peak, and therefore complicating analysis. Therefore, before performing CE-SDS and other analyses, the effects of sample preparation procedures on analytical results must be evaluated. For protein X, IAM should be excluded for CE-SDS analysis.
研究领域:
生物化学
·
蛋白质分析
·
药物开发
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Bioresource Technology Reports
·
2018
·
IF 0
Cheng Cai,Yu Jin,Yuxia Pang,Qia Ke,Wenhui Qiu,Xueqing Qiu,Yanlin Qin,Hongming Lou
Cellulase components in hydrolyzate were accurately traced by SDS-PAGE gel electrophoresis during the enzymatic hydrolysis of corncob residual (CCR) and delignified CCR (CCR-DL). After a quick initial adsorption on substrates, cellulase in hydrolyzate remained stable for CCR, but showed an obvious increasing trend for CCR-DL. It indicated that lignin in CCR was the main factor for the loss of cellulase in hydrolyzate. During CCR's hydrolysis, β‑glucosidase, xylanase , endoglucanase III (EGIII) and EGV were rapidly adsorbed on lignin and weren't released in subsequent, however, cellobiohydrolase I (CBHI), CBHII, EGI and EGIV were mainly adsorbed on cellulose in the early stage. With the hydrolysis of substrate, cellulase on cellulose would be released into hydrolyzate, but cellulase in hydrolyzate gradually became hydrophobic and much easier to be adsorb on lignin by the increasing hydrophobic interaction . These two factors dominated the change of cellulase concentration in hydrolyzate in the later stage of CCR's hydrolysis.
研究领域:
生物化学
·
分子生物学
·
植物资源利用
·
酶工程
✓ 已验证
DOI
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2017
·
IF 2.603
Wenhua Li,Bin Yang,Dongmei Zhou,Jun Xu,Wenjia Li,Wen-Chen Suen
Sodium dodecyl sulfate capillary electrophoresis (CE-SDS) has been widely used to separate and quantify monoclonal antibody (mAb) fragments and impurities. Two fragments with different masses smaller than monomer were observed in the CE-SDS analysis of a recombinant IgG1 mAb treated at 40 °C for 28 days. Reversed phase liquid chromatography mass spectrometry (RP–LC–MS) and trypsin digestion peptide mapping followed by LC–MS/MS analysis were employed to conclusively demonstrate that these antibody fragments were the degradation products derived from a Ser–Ser peptide bond cleavage at the heavy chain complementarity determining region (CDR). The total percentage of the cleaved antibody heavy chain was increased from 1.1% in the control sample to 4.6% in the forced degradation sample stored at 40 °C for 28 days by using CE-SDS analysis. Native size exclusion liquid chromatography (SEC-HPLC) was used to analyze the fragment profile of this treated mAb but did not reveal fragments smaller than the monomer. However, a small fragment was detected using denaturing size exclusion liquid chromatography (dSEC-HPLC) and was confirmed to be the N-terminal fragment of the heavy chain cleaved at the CDR region using RP–LC–MS analysis. These results suggested that this N-terminal fragment may be closely associated with the rest of the cleaved antibody molecule through non-covalent bond interactions. Moreover, when dSEC-HPLC and RP–LC–MS were used to analyze the aggregate and monomer samples individually collected from SEC-HPLC, both samples contained the same amount of the cleaved N-terminal fragment. These results suggested that the CDR region cleaved fragments likely not be involved in aggregates formation of this antibody. This case study reiterated that a combination of orthogonal analytical methods plays a crucial role in comprehensively analyzing and characterizing antibody fragmentation.
研究领域:
生物化学
·
分析化学
·
蛋白质组学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）