FOOD CHEMISTRY
·
2023
·
IF 8.8
Clement Yaw Effah,Lihua Ding,Longlong Tan,Sitian He,Xiang Li,Huijie Yuan,Yi Li,Shaohua Liu,Tongwen Sun,Yongjun Wu
Klebsiella pneumoniae ( KP ) and Acinetobacter baumannii ( AB ) are two important gram-negative bacteria that cause pneumonia and have been recently known to be associated with food. The rapid detection of these pathogens in food is important to minimize their colonization of the gut and stop new threats of the disease from spreading across the food chain. Herein, a double-edged sword aptasensor was developed for the synchronous detection of KP and AB in food and clinical samples. A highly sensitive, selective, specific, and synchronous detection of the target bacteria was achieved, and the limit of detection (LOD) was 10 cells/mL with a liner range of 50 to 10 5 cells/mL. The total assay time was 1.5 h. This study does not only provide a new tool for the detection of the target bacteria, but also serves as a promising tool for food safety and pneumonia diagnosis.
研究领域:
分析化学
·
生物传感器
·
食品安全
·
微生物学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR RESEARCH
·
2013
·Vol. 21
·期 3
·306-310
·
IF 1.639
Zhang Wen-Yi,Xiao Xin-Ze,Lv Chao,Zhao Jia,Wang Gong,Gu Xuan,Zhang Ran,Xu Bin-Bin,Zhang Dan-Dan,Li Ai-Wu,Zhang Yong-Lai,Sun Hong-Bo
Reported here is the fabrication of photopolymer hierarchical micronanostructures through a combinative process of electrospinning and subsequent photolithography. Electrospun SU-8 (epoxy-based negative photoresist) nanofiber films have been patterned into gratings with periods of 100, 200, 300, and 400 μm, respectively. Deposition of a silver nanolayer on these interlaced nanofiber films would lead to the formation of various plasmonic nanostructures, and therefore, giving rise to abundant surface-enhanced Raman scattering (SERS) “hot spots”. In the detection of Rhodamine 6G (R6G), probing molecule, the resultant SERS substrates show both high sensitivity and good reproducibility. The SERS enhancement factor could reach as high as ∼10 8 , indicating high efficiency. The fabrication of patterned, highly efficient SERS substrates may hold a great promise for the integration of SERS substrates in various microdevices such as microfluidic chips.
研究领域:
材料科学
·
光学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Runzi Zhang,Shunbi Xie,Qianyan Zhang,Ying Gao,Xiang He,Shanshan Jin,Junhao Leng,lian Zhang,Yi He
Background Chloramphenicol (CAP) is a broad-spectrum antibiotic, and its continuous use in human medicine, livestock has resulted disturbances in ecosystem stability. The complex background and low concentration of CAP in aquatic environments present significant scientific challenges for its sensitive detection. Currently detection techniques such as high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) are hindered by their complex procedures and high costs. Surface-enhanced Raman spectroscopy (SERS), due to its unique ability for fingerprint recognition, has emerged as a powerful tool for analysis and detection. However, it is often limited by the low kurtosis expression of the target. Result The water-soluble explosive polyethylenimide grafted calcium carbonate (PEI@CaCO3) microcapsule was utilized to encapsulate the signal probe Ag@4-NTP and combine it with a semiconductor SERS substrate Zn 0.995 Ce 0.005 O, enabling ultra-sensitive detection of CAP through a signal attenuation strategy. Moreover, magnetic molecularly imprinted polymers (MMIP) and nucleic acid aptamers were employed as capture probes for achieving rapid and direct magnetic separation, followed by layer-by-layer assembly to construct the sensor probe. In the presence of CAP, the aptamers and rMMIPs selectively recognized the target, forming a PEI@CaCO 3 @Ag@4-NTP@Apt@CAP@rMMIPs "sandwich" structure (Microcapsule@Apt@rMMIPs). After multiple magnetic separations, the "igniter" EDTA solution was added, causing the CaCO 3 capsules to rapidly "explode" and release a significant amount of Ag@4-NTP. This was then applied to the "signal booster" semiconductor SERS substrate. Under optimal conditions, this method exhibited a detection range of 1.0 × 10 −5 M to 1.0 × 10 −15 M, with a detection limit of 6.84 × 10 −16 M. Significant The unite of magnetic MIP and nucleic acid aptamer to form a “sandwich” structure, in combination with a semiconductor SERS substrate, not only enhances the sensor's sensitivity but also offers significant economic advantages. Moreover, this innovative technology holds great promise for accurate and highly sensitive detection of pollutants in complex environments.
研究领域:
分析化学
·
环境科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Xiaolong Fu,Huiying Wu,Zhihong Liu,Pengzhao Wang,Jiefeng Rong,Fengfu Fu,Zhenyu Lin,Yongqiang Dong
The chemical enhancement of semiconductor-based surface-enhanced Raman scattering (SERS) substrates is an exciting hot topic. Herein, a simple hydrothermal method is developed to prepare molybdenum disulfide (MoS2), which can be easily exfoliated into monolayer nanosheets (MoS2NSs) by sonication, even in the absence of any surfactant. The obtained MoS2NSs contain two types of defects, namely, one caused by the incorporation of Mo atoms of high valence states and one caused by the incorporation of S22–. The density of the two types of defects can be easily tuned by controlling the ratio of Na2S and Na2MoO4 in the raw materials. The unique properties and the clear surface make the obtained MoS2NSs ideal models to investigate the effect of defects on the SERS activity of MoS2. It is found that the SERS activity of the obtained MoS2NSs increases dramatically with the defects caused by Mo atoms of high valence states, while it first increases and then decreases with the increase of defects caused by S22–. On the basis, MoS2NSs with high SERS activity and a low detection limit of 5.0 × 10–9 mol/L toward crystal violet (CV) are obtained. Moreover, the mechanism of defects affecting the SERS activity of MoS2NSs is revealed. The defects on one hand provide a large amount of dangling bonds that can combine CV molecules to form MoS2NS–CV complex and on the other hand provide extensive induced local dipoles and enhance the overall SERS spectrum of CV.
研究领域:
材料科学
·
纳米技术
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yalei Wang,Chunning Chen,Jiaqi Liu,Jiaxin Lu,Jingtong Zhai,Hongkun Zhao,Rui Li,Nan Lu
To simultaneously possess the capability of reusability and non-destructive enrichment is very important for the SERS substrate in practical applications, but it is difficult to achieve the two capabilities simultaneously. In this work, an ordered micro-pillars array was prepared on the surface of organogel, analytes can be rapidly concentrated and enriched on the lubricating surface of the substrate, effectively avoiding the influence of “coffee ring” effect. At the same time, the pillar structures can effectively prevent the surface lubricating layer on substrate from being rapidly consumed, and it can be successfully used as a reusable supporting substrate in SERS detection, with the LOD of 5.1 × 10 −12 M and 1.1 × 10 −11 M for rhodamine 6G and crystal violet molecules.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 7
·2066-2071
·
IF 1.938
Wei Zhang,Bowei Li,Lingxin Chen,Yunqing Wang,Dingxue Gao,Xuehua Ma,Aiguo Wu
A simple and facile method has been demonstrated to fabricate low-cost surface enhanced Raman scattering (SERS) active microfluidic paper chips using a painting brush. This strategy solves the problem of mass production of highly reproducible SERS substrates without complicated or bulky micro- or nanofabrication instruments. Rhodamine 6G (R6G) was chosen as a probe molecule to evaluate the performance of the SERS active chip. To further demonstrate the possibility of this method's potential application in environmental monitoring, trace malachite green (MG) was successfully analyzed on this chip. The performance of our chips was desirable. The paper substrates with silver nanoparticles deposited by brush were found to be cost-efficient and highly sensitive (LOD for R6G and MG are 1 nM and 10 nM, respectively), and have good reproducibility (∼15% relative standard deviation).
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
CRYSTENGCOMM
·
2020
·Vol. 23
·期 3
·694-699
·
IF 3.117
Muhammad Usman Amin,Lingling Zhang,Rui Hao,Dongjie Zhang,Hongjun You,Jixiang Fang
We successfully prepared silver dendritic nanostructures with a well-defined and precisely tailored trunk and branches, as well as decorated nanoparticles, by a simple and controllable two-electrode electrochemical deposition system using different AgNO3 concentrations and deposition potentials. The prepared nanostructures are used as SERS substrates for the detection of crystal violet molecules with a minimum detection limit of 10−11 M. Finite-difference time-domain (FDTD) simulation is used to analyze the SERS activity of diverse silver dendritic nanostructures. The SERS and FDTD results revealed that the pine leaf-like structure displays excellent sensitivity and reproducibility compared to all other structures due to a large number of hotspot sites between branch–branch and branch–particle junctions. This article provides a good understanding of electromagnetic field enhancement and shows that various silver dendritic nanostructures could be potential candidates for SERS-based detection.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Nanomaterials
·
2019
·Vol. 9
·期 3
·355
·
IF 4.034
Luqiang Huang,Changji Wu,Lijuan Xie,Xue Yuan,Xinyu Wei,Qun Huang,Youqiang Chen,Yudong Lu
Nanocellulose is an abundant green resource that, owing to the larger surface area, length, and diameter of the fibers, can be used as a framework for loading Ag nanoparticles and serve as substrate for surface enhancement Raman scattering (SERS). These properties would cause the hydroxyl groups on the surface to adsorb the Ag ions and reduce them to Ag seed to form a load fulcrum. This paper presents a convenient and environmentally friendly method for the fabrication of silver-nanocellulose composites (NCF-Ag). A commonly used pesticide, carbendazim (CBZ), was used as a SERS probe to evaluate the properties of NCF-Ag. The results showed that NCF-Ag possesses good homogeneity, reproducibility, and stability. Additionally, CBZ was found to have a low limit of detection (LOD), i.e., 1.0 × 10−8M, which indicates the possibility for trace analysis. Furthermore, it presents good linearity withR2= 0.98 at 1007 and 1270 cm−1in the range from 10−4~10−7M CBZ.Keywords:nanocellulose;surface enhancement Raman scattering (SERS);carbendazim;Ag nanoparticle
研究领域:
材料科学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
OPTICAL MATERIALS
·
2022
·
IF 3.754
Xiaoxu Zhang,Chengxu Song,Shilun Li,Liang Chen,Jing Xu,Wei Liu,Shangshen Feng
The effective regulation of the absorption properties of Au nanosphere arrays, as well as effective enhancement of its SERS performance, are of great significance for its applications in the field of optical sensing. In this paper, the polyol synthesis method was used to fabricate monolayer and bilayer periodic Au nanosphere arrays, onto which monolayer graphene was coated, and the optical properties of the obtained composite structures were investigated. The results showed that the coverage of graphene enhances the absorption peak intensities of the monolayer and bilayer Au nanoarrays by up to 32.92%, and the resonance peaks were red-shifted by up to 17 nm. The Raman signals of graphene-covered monolayer and bilayer Au nanosphere composite arrays are 180 and 280 times stronger than that of pristine graphene, due to the combination of chemical enhancement and electromagnetic enhancement. And the detection sensitivity of R6G molecules by graphene-bilayer Au nanosphere composite arrays is 10 −6 M. In addition, the staggered stacking of bilayer Au nanosphere arrays can provide more plasmonic hotspots, resulting in higher Raman intensity of the substrate. These results will pave the way for the fabrication of highly efficient SERS substrates by providing various promising candidates.
研究领域:
材料科学
·
光学传感
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
NANOTECHNOLOGY
·
2021
·Vol. 32
·期 37
·375603
·
IF 3.874
Chundong Liu,Jia Li,Fengcai Lei,Yisheng Wei,Zhen Li,Chao Zhang,Qianqian Peng,Jing Yu,Baoyuan Man
The surface-enhanced Raman spectroscopy (SERS) has attracted much attention due to the powerful capability of quantificational analysis. Nowadays, most of the enhancement effect by SERS substrate is provided by the 'hot spots' occupying relatively small space. When the amount of analyte is too low, it is difficult to ensure that all the probe molecules can be placed into the 'hot spots', which is a headache in SERS quatification. In order to solve this problem, we have developed a structure of CuO nanowires/Ag nanoparticles with wettability capacity difference, which can aggregate molecules in water and oil simultaneously under two different mechanisms. The limit of detection and enhancement factor of this structure are estimated as 10−15 M and 1.55 × 1011 respectively (for rhodamine 6G, R6G). In a proof-in-principle experiment of sewage detection, it successfully achieved the aggregation and additional enhancement of both the R6G molecules in aqueous solution and thiuram molecules in toluene, realizing efficient and accurate Raman detection.
研究领域:
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiaomu Shan,Jie Pan,Xuejian Du,Jian Shi,Zhuofan Wei,Yuanze Xu,Shouzhen Jiang
The excellent performance of surface-enhanced Raman spectroscopy (SERS) is closely related to the intensity, density and uniformity of hotspots. In this study, we introduce a PET/Au NPs@COF Raman substrate featuring three-dimensional hotspots. The spacing between each layer of gold nanoparticles (NPs) is precisely controlled by adjusting the thickness of the COF via the interfacial synthesis technique, causing the strong coupling between gold particles across the layers. The porosity of the COF enables the substrate to adsorb numerous analyte molecules into the large-area uniform hotspots. Through simulations and adsorption detection on the substrate, Raman testing further demonstrated that the substrate with double-layer Au NPs@COF exhibited the most favorable SERS performance. Additionally, the substrate allows for selective detection based on the particle size of the target molecule. The flexibility of the substrate enables it to conform to irregular surfaces, effectively capturing analytes from a variety of real samples.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Theranostics
·
2022
·
IF 11.6
Bohan Yin,Qin Zhang,Xinyue Xia,Chuanqi Li,Willis Kwun Hei Ho,Jiaxiang Yan,Yingying Huang,Honglian Wu,Pui Wang,Changqing Yi,Jianhua Hao,Jianfang Wang,Honglin Chen,Siu Hong Dexter Wong,Mo Yang
Background: CRISPR-Cas12a has been integrated with nanomaterial-based optical techniques, such as surface-enhanced Raman scattering (SERS), to formulate a powerful amplification-free nucleic acid detection system. However, nanomaterials impose steric hindrance to limit the accessibility of CRISPR-Cas12a to the narrow gaps (SERS hot spots) among nanoparticles (NPs) for producing a significant change in signals after nucleic acid detection. Methods: To overcome this restriction, we specifically design chimeric DNA/RNA hairpins (displacers) that can be destabilized by activated CRISPR-Cas12a in the presence of target DNA, liberating excessive RNA that can disintegrate a core-satellite nanocluster via toehold-mediated strand displacement for orchestrating a promising “on-off” nucleic acid biosensor. The core-satellite nanocluster comprises a large gold nanoparticle (AuNP) core surrounded by small AuNPs with Raman tags via DNA hybridization as an ultrabright Raman reporter, and its disassembly leads to a drastic decrease of SERS intensity as signal readouts. We further introduce a magnetic core to the large AuNPs that can facilitate their separation from the disassembled nanostructures to suppress the background for improving detection sensitivity. Results: As a proof-of-concept study, our findings showed that the application of displacers was more effective in decreasing the SERS intensity of the system and attained a better limit of detection (LOD, 10 aM) than that by directly using activated CRISPR-Cas12a, with high selectivity and stability for nucleic acid detection. Introducing magnetic-responsive functionality to our system further improves the LOD to 1 aM. Conclusion: Our work not only offers a platform to sensitively and selectively probe nucleic acids without pre-amplification but also provides new insights into the design of the CRISPR-Cas12a/SERS integrated system to resolve the steric hindrance of nanomaterials for constructing biosensors.
研究领域:
生物技术
·
分析化学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）