PHYTOCHEMISTRY
·
2018
·
IF 3.186
Yu Dai,Yun Lin,Xiaoyan Pang,Xiaowei Luo,Limbadri salendra,Junfeng Wang,Xuefeng Zhou,Yongjun Lu,Bin Yang,Yonghong Liu
Five new peptides, Sinulariapeptides A−E together with seven known peptides ( 6–12 ) were isolated from the soft coral associated fungus Simplicillium sp. SCSIO 41209. The structures of the new compounds and their absolute configurations were established on the basis of spectroscopic analysis including NMR, MS and ECD. All the Compounds (except sinulariapeptides B−D) were tested for the inhibitory activities of Mycobacterium tuberculosis protein tyrosine phosphatase B (MptpB), and antifungal activities against five plant pathogenic fungi . Simplicilliumtides B and cyclo(L-Val-L-Pro) showed inhibitory activity with the IC 50 values of 35.0 and 25.9 μ M, sinulariapeptides A, simplicilliumtides J, verlamelins A and B exhibited potent inhibition against Colletotrichum asianum with the MIC values of range from 4.9 to 9.8 μ g/mL and simplicilliumtides J, verlamelins A and B displayed inhibition against Pyricularia oryza Cav with the MIC values in the range of 19.5–78.1 μ g/ml, respectively.
研究领域:
海洋生物学
·
天然产物化学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2024
·
IF 4
Liu Chenglong,Wen Xueer,Pan Huiqiao,Luo Ying,Zhou Junyang,Wu Yuzhe,Zeng Zhiyong,Sun Ting,Chen Jun,Hu Zhangli,Lou Sulin,Li Hui
Cobalt pollution is harmful to both the aquatic ecosystem and human health. As the primary producer of aquatic ecosystems in hypersaline environments, unicellular planktonic Dunaliella microalgae is considered to be a low-energy and eco-friendly biosorbent that removes excess cobalt and enhances the vitality of coastal and marine ecosystems. In this study, we found that the halotolerant microalga named Dunaliella sp. FACHB-558 could grow under a salinity condition with 0.5–4.5 M NaCl. A phylogenetic analysis based on the rbcL gene revealed that Dunaliella sp. FACHB-558 is a close relative of Dunaliella primolecta TS-3. At lab-scale culture, Dunaliella sp. FACHB-558 exhibited high tolerance to heavy metal stresses, including cobalt, nickel, and cadmium. Treatment with 60 μM cobalt delayed its stationary phase but ultimately led to a higher population density. Furthermore, Dunaliella sp. FACHB-558 has the ability to adsorb the cobalt ions in the aquatic environment, which was evidenced by the decreased amount of cobalt in the culture medium. In addition, the tolerance of Dunaliella sp. FACHB-558 to cobalt stress was correlated with enhanced nitric oxide content and peroxidase activity. The autophagy inhibitor 3-MA enhanced nitric oxide burst, increased peroxidase activity, and accelerated the bioremoval of cobalt, suggesting that the autophagy pathway played a negative role in response to cobalt stress in Dunaliella sp. FACHB-558. In summary, our study identified a novel microalga possessing high cobalt tolerance and provided a promising natural biosorbent for the research and application of heavy metal bioremediation technology.
研究领域:
环境科学
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2018
·
IF 6.434
Shanshan Sun,Haizhen Wang,Yuanzhi Chen,Jun Lou,Laosheng Wu,Jianming Xu
Mycobacterium sp. WY10 was a highly effective PAHs-degrading bacterium that can degrade phenanthrene (PHE, 100 mg L −1 ) completely within 60 h and 83% of pyrene (PYR, 50 mg L -1 ) in 72 h. In this study, ten and eleven metabolites, respectively, were identified in PHE and PYR degradation cultures, and a detailed PHE and PYR metabolism maps were constructed based on the metabolic results. The strain WY10 degraded PHE and PYR with initial dioxygenation mainly on 3,4- and 4,5-carbon positions, respectively. Thereafter, PYR degradation entered the PHE degradation pathway via the ortho -cleavage. It was observed that the “lower pathway” of PHE and PYR degradations were different. Based on the kinetics of residual metabolites, PHE was degraded in a dominant phthalate pathway and a minor salicylate pathway. However, both phthalate and salicylate pathways played important roles on PYR degradation. The WY10 genome revealed there were fifty-three genes related to PAHs degradations, including a complete gene set for PHE and PYR degradation via the phthalate pathway. The candidate gene/ORF, BOH72_19755, encoding salicylate synthase might contribute in the salicylate pathway.
研究领域:
环境微生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2022
·
IF 6.064
Wang Guangli,Li Xiang,Zheng Jiaxin,Li Xuedong,Bai Lingling,Yue Wenlong,Jiang Li
Diazinon is one of the most widely used organophosphate insecticides, one that is frequently detected in the environment. In this study, a diazinon-degrading bacterium, DI-6, previously isolated from diazinon-contaminated soil in China has been subsequently identified as Sphingobium sp. on the basis of its physiological and biochemical characteristics, as well as by virtue of a comparative analysis of 16S rRNA gene sequences. This strain is capable of using diazinon as its sole carbon source for growth and was able to degrade 91.8% of 100 mg L–1 diazinon over a 60-h interval. During the degradation of diazinon, the following seven metabolites were captured and identified by gas chromatography/mass spectrometry (GC–MS) analysis: diazoxon, diazinon aldehyde, isopropenyl derivative of diazinon, hydroxyethyl derivative of diazinon, diazinon methyl ketone, O-[2-(1-hydroxyethyl)-6-methylpyrimidin-4-yl] O-methyl O-hydrogen phosphorothioate, and O-(6-methyl pyrimidin-4-yl) O,O-dihydrogen phosphorothioate. Based on these metabolites, a novel microbial biodegradation pathway of diazinon by Sphingobium sp. DI-6 is proposed. This research provides potentially useful information for the application of the DI-6 strain in bioremediation of diazinon-contaminated environments.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
AQUACULTURE
·
2016
·
IF 1.893
Ningning Liu,Shanshan Zhang,Weiwei Zhang,Chenghua Li
Recently, the use of probiotics as an alternative strategy to control bacterial diseases in aquaculture has garnered much attention due to the merits of environmental friendliness. In this study, a bacterium identified as Vibrio sp. V33 was isolated from healthy sepia and was determined to have strong antagonistic activity towards the pathogenic isolate Vibrio splendidus Vs using the disk diffusion method. The antagonistic substance was secreted into the supernatant and was thermo-stable. The antagonistic activity of Vibrio sp. V33 may be attributed to its higher ability to compete for iron than that of V . splendidus Vs, as deduced from siderophore quantification using the CAS method. Number of colony counts from seawater and Apostichopus japonicus infection experiments showed that Vibrio sp. V33 significantly decreased the quantity of V . splendidus Vs in natural seawater and promoted the survival rate of A . japonicus infected by V . splendidus , with a relative percentage survival of 43% under the current conditions. Our findings suggest that V33 could be potentially used as potential bacterial antagonist for controlling V . splendidus infections in sea cucumber aquaculture. Statement of relevance It might protect A . japonicus from V . splendidus infection.
研究领域:
水产养殖学
·
微生物学
✓ 已验证
DOI
高影响因子
AMB Express
·
2019
·Vol. 9
·期 1
·1-9
·
IF 2.226
Zhao Shuxue,Hu Chunhui,Guo Lizhong,Li Kuiran,Yu Hao
A 3-hydroxypyridine degrading bacterium, designated strain DW-1, was isolated from petroleum contaminated soil in Liao River China. 16S rRNA-based phylogenetic analysis indicates that strain DW-1 belongs to genus Agrobacterium. The optimal cultivation temperature and pH for strain DW-1 with 3-hydroxypyridine were 30 °C and 8.0, respectively. Under optimal conditions, strain DW-1 could completely degrade up to 1500 mg/L of 3-hydroxypyridine in 66 h. The 3-hydroxypyridine degradation pathway of strain DW-1 was suggested by HPLC and LC–MS analysis. The first reaction of 3-hydroxypyridine degradation in strain DW-1 was α-hydroxylation so that the major metabolite 2,5-dihydroxypyridine was produced, and then 2,5-dihydroxypyridine was transformed by a Fe2+-dependent dioxygenase to form N-formylmaleamic acid. N-Formylmaleamic acid will be transformed to maleic acid and fumaric acid through maleamic acid. This is the first report of the 3-hydroxypyridine degradation pathway and the utilization of 3-hydroxypyridine by a Agrobacterium sp. It may be potentially used for the bioremediation of environments polluted with 3-hydroxypyridine.
研究领域:
微生物学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Qi Han,Youwen Xing,Lulu Yan,Lulu Cao,Zehao Li,Jiandong Jiang,Xihui Xu,Chen Chen
Cyromazine, a triazine insecticide, raises food safety concerns due to residues in vegetables like cowpeas. Microbial metabolism is key for pesticide elimination, but bacteria efficient in cyromazine degradation are limited, with uncharacterized enzymes. This study isolated a highly efficient cyromazine-degrading bacterium, Mycobacterium sp. M15, from a cowpea field. M15 utilized cyromazine as the sole carbon source for its growth and completely degraded 0.5 mM cyromazine within 24 h. The degradation pathway involved hydrolyzing cyromazine to N-cyclopropylammeline and further to N-cyclopropylammelide, with amino groups removed sequentially. The cyclopropylamine group in N-cyclopropionamide continued to hydrolyze to cyanuric acid. A protein, CriA, identified as an aminohydrolase in M15, degraded cyromazine to N-cyclopropylammeline. Using CriA reduced cyromazine residues on cowpea surfaces and completely degraded them in immersion solutions. These findings offer insights into cyromazine’s microbial degradation mechanism and highlight the potential of cyromazine-degrading enzymes in enhancing food safety.
研究领域:
环境科学
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微生物学
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生物技术
·
农药化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2018
·
IF 5.807
Xinru Zhang,Hao Yuan,Zeyi Jiang,Dahao Lin,Xinxin Zhang
The organic matter and surfactants in wastewater may cause variations in the surface tension of wastewater (STW) ranging between ∼40 and ∼70 mJ·m −2 . This study focused on the influence of STW on microalgae biofilm formation. A theoretical analysis was first conducted, and then microalgae biofilm formation on hydrophilic and hydrophobic substrata in liquid and real wastewater with different surface tensions was studied. The results demonstrated that STW affected microalgae biofilm formation remarkably. When the surface tension of liquid medium ( γ lv ) was approximately equal to the average value of surface free energy of microalgae and substrata, biofilm formation reached the minimum. Microalgae biofilm formation on a hydrophilic surface first decreased (from ∼2200 to ∼1500 cells/mm 2 ) and then increased (from ∼1500 to 3100 cells/mm 2 ) with the decrease in γ lv (from ∼70 to ∼40 mJ·m −2 ), whereas biofilm on a hydrophobic surface continued to decrease (from ∼2500 to 1000 cells/mm 2 ).
研究领域:
环境生物学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Wenshan He,Run Jiang,Siqi Li,Minglu Zhang,Tingting Zhang,Xiaobiao Zhu,Xiaohui Wang
Chlortetracycline (CTC), a widely used typical tetracycline antibiotic, has raised increasing concerns due to its potential health and environmental risks. Biodegradation is considered an effective method to reduce CTC in environment. In this study, a strain Aspergillus sp. LS-1, which can efficiently degrade CTC, was isolated from CTC-rich activated sludge . Under optimal conditions, the maximum removal efficiency of CTC could reach 95.41%. Temperature was the most significant factor affecting the degradation efficiency of LS-1. The 19 products were identified in the CTC degradation by strain LS-1, and three degradation pathways were proposed. All the degradation pathways for CTC exhibited ring-cleaving, which may accelerate the mineralization of CTC. To gain more comprehensive insights into this strain, we obtained the genome of LS-1, which had high GC content (50.1%) and completeness (99.3%). The gene annotation revealed that LS-1 contains some vital enzymes and resistance genes that may carry functional genes involved in the CTC degradation. In addition, other antibiotic resistance genes were found in the genome of LS-1, indicating that LS-1 has the potential to degrade other antibiotics. This study provides a more theoretical basis for the investigation of CTC degradation by fungi and new insights into the biodegradation of CTC.
研究领域:
环境科学
·
微生物学
·
基因组学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Yidong Zhou,Zhijian Ke,Jiazhuo Zhang,Wenbin Chang,Yarong Cui,Yimiao Chen,Yirui Huang,Chunli Xia,Wenjia Zhong,Zifa Deng,Dayong Jin
Microorganisms are constantly evolving to resist the toxicity of artificial antibiotics and preservatives released into the environment. Parabens, a group of alkyl and aryl esters of p -hydroxybenzoate, are a class of widely used antimicrobial preservatives, particularly effective against Gram-positive bacteria and fungi. Previous studies have reported the degradation of parabens by numerous Gram-negative strains and activated sludge, with degradation processes often involving transesterification. However, the physiological significance of transesterification has received little attention. Here, a Gram-positive bacterium, Microbacterium sp. S1037 was isolated and characterized for its ability to degrade eight parabens and completely mineralize them through p -hydroxybenzoate and protocatechuate ( β -ketoadipate pathway). Moreover, strain S1037 also possesses the ability of paraben-transesterification. Alcohols compete with water molecules for the active center of enzymes, accelerating the transesterification of parabens and inhibiting hydrolysis. Considering that the fate of the transesterification product is still hydrolysis, we speculated and proved that such a “superfluous” mechanism is a detoxification strategy, and could be used in the bioremediation of parabens in wastewater. Microbial transesterification of parabens has been reported worldwide, and we speculated that it might be a common detoxification choice in the evolution of microorganisms worldwide.
研究领域:
微生物学
·
环境科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 3.978
Shuiquan Chen,Meng Zang,Lin Li,Juntao Chen,Qiyou Liu,Xiwen Feng,Shuo Sun,Chuanwei Zang,Chaocheng Zhao
Biodesulfurization is a promising, environmentally friendly method to desulfurize the organosulfur compounds in crude oils through the highly selective cleavage of carbon-sulfur bonds under mild reaction conditions. In this study, the behavior of a hydrophobic bacterium, Gordonia sp. SC-10, was evaluated in the presence of diesel oils. The biodesulfurization pathways, bacterium hydrophobicity , and cell ultrastructure were investigated, and the capability of strain SC-10 to desulfurize diesel oil was also studied. The results showed that strain SC-10 can desulfurize organosulfur compounds through sulfur-specific desulfurization pathways by producing corresponding hydroxylated products in oil-water polyphasic systems. Mycolic acids consisting of 47 and 58 carbon atoms chains were produced on the cell surface, and inclusion bodies were produced in the cells, both of which may improve the bioavailability of the substrates, thereby enhancing its adaptability to oil-water polyphasic systems. To our knowledge, this is the first report of intracellular inclusion body formation during biodesulfurization. Strain SC-10 also exhibited broad substrate specificity towards organosulfur compounds and effectively desulfurized diesel oils. The sulfur content in real-life diesel oil was reduced from 167.7 mg/L to 19.7 mg/L, with 88.3% of sulfur removed after five days of biodesulfurization. This study showed that Gordonia sp. SC-10 can be effectively used for the biodesulfurization of diesel oils.
研究领域:
微生物学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2015
·Vol. 31
·期 4
·645-650
·
IF 0.875
Geng Zhi,Zhu Suiyi,Yu Yang,Lu Ying,Lin Rao,Guo Shujun,Bian Dejun,Yang Xia,Huo Mingxin,Huo Hongliang
Novel matrix beads for the immobilization of strain Comamonas testosteroni sp. bdq06 to degrade quinoline were fabricated from polyethersulfone(PES). The beads have an average size of 3 mm and a surface dense layer of 20 microns. To help adhesion and proliferation of bacterial cells, the surfaces of the PES beads were etched, and numerous holes about 1.5 micrometers in diameter were generated as tunnels for cell colonizing in the larger internal cavities of about 5 micrometers in diameter. The quinoline degradation was remarkably enhanced by the cells immobilized in PES beads compared with that by the free cells at pH 5.0 or 10.0 and a temperature of 40 °C. The enhanced degradation of quinoline was contributed to the biofilm on the surface of PES beads, resulting in the significant reduction of retention time from 9 h to 2 h. Furthermore, the beads remain intact after the ultrasonic treatment of them for 30 min or recycling 50 times, indicating that they have excellent mechanical strength, flexibility and swelling capacity. Thus, PES beads have great potential to be matrix for the cell immobilization in bioaugmentation.
研究领域:
环境生物学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）