ACS Applied Nano Materials
·
2024
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IF 5.3
Cailin Qiao,Xinrou Wang,Yuwei Gao,Yi Ma,Huibo Luo,Suyi Zhang,Danqun Huo,Changjun Hou
A method was proposed to enhance the growth of Saccharomyces cerevisiae cells and increase the alcohol yield. This strategy utilized nanozymes, specifically FeCo-Prussian blue analogue nanoparticles (FeCo-PBA NPs), FeCo-N-C, CoSO porous nanocubes (CoSO PNCs), and FeN3P-single-atom nanozymes (FeN3P-SAzyme), to mimic the antioxidant enzymes. When subjected to H2O2 stress, the nanozymes significantly stimulated cell growth and effectively reduced the levels of reactive oxygen species (ROS), as evidenced by the decrease in the malondialdehyde (MDA) content. Additionally, the decline in lactate dehydrogenase (LDH) activity within the fermentation environment suggested that nanozymes provided protection against cell membrane damage caused by H2O2. This protective effect can be attributed to their inhibitory impact on ROS production. Further results demonstrated that 50 μg mL–1 of nanozymes led to an increase in the ethanol yield and total alcohol yield in S. cerevisiae. This approach held great potential for enhancing alcohol production efficiency in the S. cerevisiae industrial fermentation process.
研究领域:
微生物学
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生物技术
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生物化学
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发酵科学
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DOI
高影响因子
Nature Communications
·
2026
·Vol. 17
·期 1
·1806
·
IF 18.1
Zhong Wei,Liu Nana,Chen Binbin,Sun Huiqi,Fei Xiao,Lian Jiazhang,Guo Junling,Wang Bo,Wang Yajie
Methanol is a promising one-carbon (C1) feedstock for microbial bioconversion; however, engineered Saccharomyces cerevisiae often faces energetic constrains during its assimilation. Here, we develop SC-AOX 25 , an energy-efficient methylotrophic S. cerevisiae , through engineering of heterologous methanol-formaldehyde-formate (MFF) oxidation pathways coupled with adaptive laboratory evolution. SC-AOX 25 efficiently generates adenosine triphosphate (ATP) and nicotinamide adenine dinucleotide (NADH) during methanol metabolism while co-assimilating methanol-derived intermediates (formaldehyde, formate, and CO₂) via native glyoxylate-serine cycle, pentose phosphate pathway, and reductive glycine pathway. Key energy modules - Fdh1 sc , Adh2 m , Aox m , and Rgi2 m - are characterized for their roles in ATP/NADH synthesis and methylotrophic growth. Formaldehyde-induced DNA-protein crosslinks (DPCs) and large repeated DNA fragments suggest strategies for methanol detoxification and phenotype enhancement. Utilizing SC-AOX 25 , we enable CO₂ assimilation through non-native Calvin cycle during methanol fermentation, establishing the engineered strain as a robust and energy-efficient methylotrophic platform for further C1 engineering.
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DOI
高影响因子
AMB Express
·
2024
·Vol. 14
·期 1
·1-10
·
IF 3.5
Wang Laiyou,Liu Bingbing,Meng Qingshan,Yang Chunchun,Hu Yiyi,Wang Chunyan,Wu Pengyu,Ruan Chen,Li Wenhuan,Cheng Shuang,Guo Shuxian
The unsustainable and widespread utilization of fossil fuels continues to drive the rapid depletion of global supplies. Biodiesel has emerged as one of the most promising alternatives to conventional diesel, leading to growing research interest in its production. Microbes can facilitate the de novo synthesis of a type of biodiesel in the form of fatty acid methyl esters (FAMEs). In this study, Saccharomyces cerevisiae metabolic activity was engineered to facilitate enhanced FAME production. Initially, free fatty acid concentrations were increased by deleting two acetyl-CoA synthetase genes (FAA1, FAA4) and an acyl-CoA oxidase gene (POX1). Intracellular S-adenosylmethionine (SAM) levels were then enhanced via the deletion of an adenosine kinase gene (ADO1) and the overexpression of a SAM synthetase gene (SAM2). Lastly, the S. cerevisiae strain overproducing free fatty acids and SAM were manipulated to express a plasmid encoding the Drosophila melanogaster Juvenile Hormone Acid O-Methyltransferase (DmJHAMT). Using this combination of engineering approaches, a FAME concentration of 5.79 ± 0.56 mg/L was achieved using these cells in the context of shaking flask fermentation. To the best of our knowledge, this is the first detailed study of FAME production in S. cerevisiae. These results will provide a valuable basis for future efforts to engineer S. cerevisiae strains for highly efficient production of biodiesel.
研究领域:
生物技术
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代谢工程
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可再生能源
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Bin Ma,Min Liu,Zhen-Hai Li,Xinyi Tao,Dong-Zhi Wei,Feng-Qing Wang
Patchoulol, a natural sesquiterpene compound, is widely used in perfumes and cosmetics. Several strategies were adopted to enhance patchoulol production in Saccharomyces cerevisiae: (i) farnesyl pyrophosphate (FPP) synthase and patchoulol synthase were fused to increase the utilization of FPP precursor; (ii) expression of the limiting genes of the mevalonate pathway was enhanced; (iii) squalene synthase was weakened by a glucose-inducible promoter of HXT1 (promoter for hexose transporter) to reduce metabolic flux from FPP to ergosterol; and (iv) farnesol biosynthesis was inhibited to decrease the consumption of FPP. Glucose was used to balance the trade-off between the competitive squalene and patchoulol pathways. The patchoulol production was 59.2 ± 0.7 mg/L in a shaken flask with a final production of 466.8 ± 12.3 mg/L (20.5 ± 0.5 mg/g dry cell weight) combined with fermentation optimization, which was 7.8-fold higher than the reported maximum production. The work significantly promoted the industrialization process of patchoulol production using biobased microbial platforms.
研究领域:
生物技术
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代谢工程
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生物过程工程
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DOI
PubMed
高影响因子
Food Bioscience
·
2026
·
IF 6.2
Zhiqiang Yi,Weizhong Hu,Huiying Li,Shuxin Zhou,Jing Chengjun,Quang D. Nguyen,Szilárd Kun,Szandra Klátyik,Zhirong Wang,Jianquan Kan,Muying Du
In this study, four Saccharomyces cerevisiae strains were evaluated for their fermentation performance and the resulting wine’s quality. The results demonstrated that the Saccharomyces cerevisiae SY exhibited the most robust fermentation capacity, with faster initiation and higher efficiency. The SY group exhibited a higher total content of active ingredients (1.04 mg/mL), with Parishin E levels being significantly higher compared to the others. Although the amino acid content of PM group was found to be the highest (63.36 mg/L), while the percentage of bitter amino acid in the SY group was found to be the lowest (39.52%). and the percentage of umami amino acid in the SY group was found to be the highest (28.1%). The DV10 group exhibited the highest levels of pyruvic and malic acid (0.53 g/L and 1.64 g/L, respectively) while the SY group demonstrated the lowest acetic acid content (1.63 g/L). In addition, the SY group had a more complex aroma composition, with higher scores for sensory floral and fruity aromas. Furthermore, Gastrodia elata fermentation wine could reduce the damage of nerve cells in sleep-disordered zebrafish, reduce the distance and time of zebrafish's nocturnal movement, which had effect of improving sleep in zebra fish model, Specific mechanisms of action can be further investigated in the future.
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高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·1-11
·
IF 3.9
Wahid Ehtisham,Ocheja Ohiemi Benjamin,Oguntomi Sunday Olakunle,Pan Run,Grattieri Matteo,Guaragnella Nicoletta,Guaragnella Cataldo,Marsili Enrico
Electrodes functionalised with weak electroactive microorganisms offer a viable alternative to conventional chemical sensors for detecting priority pollutants in bioremediation processes. Biofilm-based biosensors have been proposed for this purpose. However, biofilm formation and maturation require 24–48 h, and the microstructure and coverage of the electrode surface cannot be controlled, leading to poorly reproducible signal and sensitivity. Alternatively, semiconductive biocompatible coatings can be used for viable cell immobilization, achieving reproducible coverage and resulting in a stable biosensor response. In this work, we use a polydopamine (PDA)-based coating to immobilize Saccharomyces cerevisiae yeast viable cells on carbon screen printed electrodes (SPE) for Cu(II) detection, with potassium ferricyanide (K 3 [Fe (CN) 6 ]) as a redox mediator. Under these conditions, the current output correlates with Cu (II) concentration, reaching a limit of detection of 2.2 µM, as calculated from the chronoamperometric response. The bioelectrochemical results are supported by standard viability assays, microscopy, and electrochemical impedance spectroscopy. The PDA coatings can be functionalised with different mutant strains, thus expanding the toolbox for biosensor design in bioremediation.
研究领域:
微生物学
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环境科学
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生物技术
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Shen Yao,Yang Li,Xianwei Zeng,Yuan Cui,Zhaolian Li,Meichen Liu,Daqing Zhao,Shiting Yu,Siming Wang
Fermentation affects ginseng wine flavors. In this study, the kinetic profiles of volatile and non-volatile flavor compounds generated during the fermentation of ginseng wine were monitored by two-dimensional gas chromatography-time-of-flight mass spectrometry and liquid chromatography–mass spectrometry. A sensory analysis revealed that the sample fermented for 7 days had the best flavor balance (prominent fruity aroma and minor herbal notes). Five key flavor compounds were identified: isoamyl alcohol, 2-methylbutyraldehyde, isobutyl acetate, 2-nonenal, and heptanal. Notably, 2-nonenal and heptanal, which are unique ginseng flavor compounds, produced a distinct fatty–fruity composite aroma during fermentation. Correlation analyses indicated that organic acids promoted the production of flavor compounds in ginseng wine and that amino acids served as flavor compound precursors. Sensory reconstruction experiments using exogenous flavor compounds showed that these five flavor compounds significantly affected ginseng wine aromas, especially fatty, fruity, and sweet aromas. These results guide industrial production of high-quality, flavorful, low-alcohol ginseng wine.
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DOI
PubMed
高影响因子
Fermentation-Basel
·
2025
·Vol. 11
·期 12
·664
·
IF 3.3
Yu Hou,Yubo Wang,Tianpeng Ma,Shuobo Shi,Zheng Wang,Chun Shen,Yunming Fang,Fenghui Ye,Zihe Liu
Fossil fuel overuse drives excessive CO2emissions, exacerbating environmental degradation and climate change. Coupling electrochemistry with microbial fermentation provides a promising route to convert CO2into fuels and chemicals. However, microbial electrolytic solution tolerance remains a critical bottleneck, as observed in model organisms likeSaccharomyces cerevisiae(S. cerevisiae). To address this, we engineeredS. cerevisiaeto utilize electrochemically derived formate, thereby boosting free fatty acids (FFAs) production. By optimizing culture conditions and heterologously expressing formate dehydrogenase (FDH), we improved formate assimilation efficiency. Additionally, we introduced stress-resistant genes for a better electrolytic solution tolerance to sustain growth and FFAs synthesis under harsh electrolytic conditions (e.g., high formate/salt ion concentrations), eliminating the need to separate formate from the electrolyte post-electrolysis. In the presence of 4 g/L formate electrolytic medium, the engineered strain YB061 achieved a 41.9% increase in biomass and a formate conversion rate exceeding 97.0%. Compared to the parental strain, YB061 enhanced FFAs production by 92.8% by utilizing formate-containing electrolytes, demonstrating great potential for bio-electrochemical manufacturing. However, further work is needed to improve yeast tolerance to high formate concentrations and to enable direct coupling of CO2electroreduction with microbial cultivation.
研究领域:
合成生物学
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生物化学工程
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微生物代谢
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电化学
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可持续能源
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工业生物技术
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DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 3.8
Junjie Wang,Pengming Yang,Shuai Tu,Weihong Zhong
Scopoletin, a plant-derived coumarin, exhibits diverse pharmacological activities. Nowadays, scopoletin is mainly produced by plant extraction. In this study, a Saccharomyces cerevisiae strain capable of de novo scopoletin biosynthesis was constructed by successively integrating ten heterologous genes into a p -coumaric acid producing strain. The following strategies were employed to improve the yield of scopoletin: (1) A high-yield caffeic acid platform strain (301.98 mg/L) was established by increasing the HpaBC copy number, integration of EcaroL and RgTAL , feedback-resistant mutant expression cassette ( ARO3 D154N / ARO4 G141S / ARO7 K229L ), and knockout competing pathway genes ( ARO10 / PDC5 ); (2) Two distinct pathways from caffeic acid to scopoletin were introduced and selected; (3) Integration of heterologous genes AtHCT and AtC3’H , to construct the pathway from p -coumaroyl-CoA to caffeoyl-CoA; (4) Attenuating PYK1 by introducing the mutant gene of PYK1 D146N to increase PEP supply; (5) Overexpression of cofactor regeneration genes ( ADO1 , SAH1 , and ZWF1 ) to strengthen the supply of SAM and NADPH; (6) Adjustment of the fermentation medium. The engineered strain produced scopoletin of 55.32 mg/L in shake flasks. To our knowledge, this is the first report of de novo scopoletin biosynthesis in S. cerevisiae . These results establish new insights for microbial production of scopoletin and coumarin derivatives.
研究领域:
合成生物学
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代谢工程
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微生物生物技术
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天然产物生物合成
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发酵技术
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产品关联
DOI
高影响因子
ACS Synthetic Biology
·
2024
·
IF 3.7
Mei Tang,Xianhao Xu,Yanfeng Liu,Jianghua Li,Guocheng Du,Xueqin Lv,Long Liu
Betulinic acid (BA) is a lupane-type triterpenoid with potent anticancer and anti-HIV activities. Its great potential in clinical applications necessitates the development of an efficient strategy for BA synthesis. This study attempted to achieve efficient BA biosynthesis in Saccharomyces cerevisiae using systematic metabolic engineering strategies. First, a de novo BA biosynthesis pathway in S. cerevisiae was constructed, which yielded a titer of 14.01 ± 0.21 mg/L. Then, by enhancing the BA synthesis pathway and dynamic inhibition of the competitive pathway, a greater proportion of the metabolic flow was directed toward BA synthesis, achieving a titer of 88.07 ± 5.83 mg/L. Next, acetyl-CoA and NADPH supply was enhanced, which increased the BA titer to 166.43 ± 1.83 mg/L. Finally, another BA synthesis pathway in the peroxisome was constructed. Dual regulation of the peroxisome and cytoplasmic metabolism increased the BA titer to 210.88 ± 4.76 mg/L. Following fed-batch fermentation process modification, the BA titer reached 682.29 ± 8.16 mg/L. Overall, this work offers a guide for building microbial cell factories that are capable of producing terpenoids with efficiency.
研究领域:
生物技术
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代谢工程
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药学
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微生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 10
·9033
·
IF 5.6
Weilan Piao,Chong Li,Pengkun Sun,Miaomiao Yang,Yansong Ding,Wei Song,Yunxiao Jia,Liqun Yu,Yanming Lu,Hua Jin
As a master regulator in cells, RNA-binding protein (RBP) plays critical roles in organismal development, metabolism and various diseases. It regulates gene expression at various levels mostly by specific recognition of target RNA. The traditional CLIP-seq method to detect transcriptome-wide RNA targets of RBP is less efficient in yeast due to the low UV transmissivity of their cell walls. Here, we established an efficient HyperTRIBE (Targets of RNA-binding proteins Identified By Editing) in yeast, by fusing an RBP to the hyper-active catalytic domain of human RNA editing enzyme ADAR2 and expressing the fusion protein in yeast cells. The target transcripts of RBP were marked with new RNA editing events and identified by high-throughput sequencing. We successfully applied HyperTRIBE to identifying the RNA targets of two yeast RBPs, KHD1 and BFR1. The antibody-free HyperTRIBE has competitive advantages including a low background, high sensitivity and reproducibility, as well as a simple library preparation procedure, providing a reliable strategy for RBP target identification inSaccharomyces cerevisiae.Keywords:HyperTRIBE;RNA-binding protein (RBP);yeast;KHD1;BFR1
研究领域:
分子生物学
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基因组学
·
酵母遗传学
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DOI
PubMed
高影响因子
Biotechnology Journal
·
2023
·
IF 4.7
Haiquan Yang,Kunjie Zhang,Wei Shen,Yuanyuan Xia,Yiting Li,Xianzhong Chen
Cembratriene-ol is a good biodegradable biopesticide ingredient with future potential applications in the field of sustainable agriculture. Cembratriene-ol is a monocyclic diterpenoid compound that is synthesized only in the trichome gland of Nicotiana plants. In this study, geranylgeranyl diphosphate synthase gene ggpps from Taxus canadensis and cbts*Δp were heterologously expressed in Saccharomyces cerevisiae W303-1A to successfully synthesize cembratriene-ol. The titer of cembratriene-ol was increased by 1.84-fold compared to the control by overexpressing the S. cerevisiae bifunctional (2E,6E)-farnesyl diphosphate synthase genes ERG20 and cbts*Δp under one promoter P GAP . The titer of cembratriene-ol in the engineered S. cerevisiae BY4741 was increased by 1.39-fold compared to the engineered S. cerevisiae W303-1A. The titer of cembratriene-ol in the engineered S. cerevisiae BY4741 was increased by 2.22-fold compared to the control by overexpressing ERG20 and cbts*Δp , respectively, using two promoters P GAP . Cembratriene-ol was found to be successfully synthesized via the integrated expression of cbts*Δp , ggpps and ERG20 on the genome of S. cerevisiae BY4741. The titer of cembratriene-ol in S. cerevisiae S25 was further increased by 1.80-fold compared to the control via dynamic control of the squalene synthase gene ERG9 . Overexpression of the genes cbts*Δp and ggpps using pY26-GPD-TEF in S. cerevisiae S25 with their integration expression increased the titer of cembratriene-ol by 26.1-fold compared to S. cerevisiae S25. The titer of cembratriene-ol was significantly enhanced by mitochondrial compartmentalized expression of cbts*Δp and ggpps , which was 76.3-fold higher than that of the initial strain constructed. It was indicated that the systematic optimization has great potential in facilitating high-level production of cembratriene-ol production in S. cerevisiae . This article is protected by copyright. All rights reserved
研究领域:
生物技术
·
微生物学
·
植物科学
·
分子生物学
·
代谢工程
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产品关联
DOI
PubMed
高影响因子
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