查找使用阿拉丁产品的科研文献

Three-phase solvent bar liquid-phase microextraction combined with high-performance liquid chromatography to determine sarcosine in human urine

Yuan Huang,Xiaobing Huang,Liping Huang,Qicai Liu,Yun Lei,Lijuan Yang,Liying Huang

Sarcosine is a potential prostate cancer marker. In this study, we developed a method of three-phase solvent bar liquid-phase microextraction combined with high-performance liquid chromatography to determine sarcosine after derivatization with 4-dimethylarminoazobenzene-4-sulfonyl chloride from human urine. The effects of different extraction conditions on extraction efficiency were investigated and optimized. Under optimum experimental conditions, a calibration graph exhibited linearity over the range of 0.05–25 μmol/L with a correlation coefficient ( r 2 ) of 0.9990. The enrichment factor was 168, and the detection limit was 0.02 μmol/L. The method was successfully used to analyze sarcosine in human urine and non-invasive detection, and good spiked recoveries ranging from 90.5 to 93.6% were obtained. The proposed method exhibited high sensitivity, high enrichment factor, good precision, and a simple setup. It may contribute to the early accurate diagnosis and the progression monitoring of prostatic carcinoma.

Sarcosine sensitizes lung adenocarcinoma to chemotherapy by dual activation of ferroptosis via PDK4/PDHA1 signaling and NMDAR-mediated iron export

Shan Guangyao,Bian Yunyi,Ren Shencheng,Hu Zhengyang,Pan Binyang,Zeng Dejun,Zheng Zhaolin,Fan Hong,Bi Guoshu,Yao Guangyu,Zhan Cheng

Background Ferroptosis, a regulated cell death driven by iron-dependent lipid peroxidation, is associated with chemoresistance in lung adenocarcinoma (LUAD). This study aims to investigate the role of sarcosine in ferroptosis and its underlying mechanisms.Methods An RSL3-induced ferroptosis model was used to screen a library of 889 human endogenous metabolites and metabolomic profiling was harnessed to identify metabolites associated with ferroptosis. Cell viability, lipid-reactive oxygen species (ROS), ferrous iron, malondialdehyde (MDA), and mitochondrial integrity were assessed to evaluate sarcosine’s effects on ferroptosis. Metabolic fate was studied using 15N-labeled sarcosine. Next, we used untargeted metabolomic profiling and next-generation sequencing to dissect metabolic and transcriptomic changes upon sarcosine supplementation. The effects of sarcosine on ferroptosis and chemotherapy were further validated in patient-derived organoids (PDOs), xenograft models, and LUAD tissues.Results Sarcosine emerged as a potent ferroptosis inducer in the metabolic library screening, which was further confirmed via cell viability, lipid-ROS, ferrous iron, and MDA measurements. Metabolic flux analysis showed limited conversion of sarcosine to other metabolites in LUAD cells, while untargeted metabolomic profiling and seahorse assays indicated a metabolic shift from glycolysis to oxidative phosphorylation. Sarcosine enhanced pyruvate dehydrogenase activity to generate more ROS by interacting with PDK4, reducing PDHA1 phosphorylation. As a co-activator of N-methyl-D-aspartate receptor (NMDAR), sarcosine also exerted its pro-ferroptosis effect via regulating ferrous export through the NMDAR/MXD3/SLC40A1 axis. Given the significance of ferroptosis in chemotherapy, we validated that sarcosine enhanced the sensitization of cisplatin by promoting ferroptosis in LUAD cells, PDOs, and xenograft models.Conclusion Sarcosine promotes ferroptosis and enhances chemosensitivity, suggesting its potential as a therapeutic agent in treating LUAD.

A photoelectrochemical self-powered sensor for the detection of sarcosine based on NiO NSs/PbS/Au NPs as photocathodic material

Hanyu Wang,Yanting Qi,Dan Wu,Qin Wei

In this study, lead(II) sulfide (PbS) nanocrystals were modified on nickel(II)oxide nanosheets (NiO NSs) via the chemical bath method. Afterwards, Au nanoparticles (NPs) were also modified successfully. A photoelectrochemical (PEC) self-powered platform for detecting sarcosine with high PEC activity was constructed. The capacity of NiO NSs to be loaded with other sensitizing materials was mainly attributed to its porous structure and large specific surface area. Under optimum conditions, the constructed PEC self-powered cathodic sensor for detecting sarcosine exhibited a linear range in 5.0 × 10 −8 –5.0 × 10 −2 mol/L with a detection limit (LOD) of 1.7 × 10 −8 mol/L. The biosensor demonstrated good reproducibility, acceptable stability and high specificity, thus confirming its potential application in the detection of other similar substances.

Facile synthesis and ozonation of carbon dots using mango pulps for dual-mode colorimetry and ratiometric fluorescence detection of sarcosine

Weidong Qin,Hongyuan Tian,Zhao Meng,Zhanqiu Tang,Junhua Wang,Zhenglong Wu

We synthesized excitation-dependent fluorescent carbon dots (CDs) through a one-pot hydrothermal process using mango pulps as precursors and discovered that the ozone-treated CDs (O 3 -CDs) could stabilize the diimine form of oxidized 3,3′,5,5′-tetramethylbenzidine (diimine oxTMB). A dual-mode colorimetry and ratiometric fluorescence method using O 3 -CDs and TMB as probes was developed for detecting sarcosine. During measurement, sarcosine was selectively oxidized by sarcosine oxidase, and the resulting hydrogen peroxide was catalyzed by horseradish peroxidase to convert TMB into a blue-colored oxidized product. Under acidic conditions, this product was further oxidized to diimine oxTMB, which exhibited an absorbance band at 450 nm. Due to the inner filter effect, the fluorescence emission of O 3 -CDs at 450 nm was significantly quenched by the diimine oxTMB, while the emission at 550 nm was utilized as a reference signal. The dual-mode method selectively detected sarcosine, with detection limits of 0.034 µM and 0.096 µM for colorimetry and ratiometric fluorescence detection, respectively. The ratiometric fluorescence sensing strategy provided a built-in correction to prevent errors originating from environmental variabilities. Moreover, the stabilization of diimine oxTMB mediated by O 3 -CDs improved the detection performance of the method. This study enriches the library of luminescent CDs through the rational utilization of natural biomass and introduces a novel approach to improve the analytical performance of dual-mode detection.

Direct electrochemistry of sarcosine oxidase on graphene, chitosan and silver nanoparticles modified glassy carbon electrode and its biosensing for hydrogen peroxide

Yunlei Zhou,Huanshun Yin,Xiaomeng Meng,Zhenning Xu,Yanrong Fu,Shiyun Ai

The direct electrochemistry of sacrosine oxidase (SOX) immobilized on graphene, chitosan and silver nanoparticles (AgNPs) modified glassy carbon electrode was realized. SOX exhibits a pair of well-defined reversible redox peaks in 0.1   M pH 7.0 phosphate buffer solution (PBS) with the oxidation peak potential of −0.446   V and the reduction peak potential of −0.496   V. The immobilized SOX retains its bioactivity, and exhibits a surface confined, reversible two-proton and two-electron transfer reaction. It also has good stability, and a fast heterogeneous electron transfer rate with the rate constant ( k s ) of 1.8   s −1 . The surface coverage of SOX is 1.18   ×   10 −7   mol/cm 2 . This biosensor shows excellent sensitivity towards H 2 O 2 in 0.1   M PBS (pH 7.0) with a wide linear range from 1.0 to 177   μM. The detection limit is 1.0   μM. The apparent Michaelis–Menten constant for immobilized SOX is calculated to be 0.18   mM. The excellent performance of the biosensor is attributed to large specific surface area and high conductivity of graphene and AgNPs, and good biocompatibility of chitosan, which improve the enzyme absorption and promote direct electron transfer between SOX and the electrode surface.

The synergetic collecting effect of N-oleoyl sarcosine (N-OSS) and dodecylamine (DDA) on the selective flotation of spodumene from albite

Hui Shao,Bingqiao Yang,Huihua Luo,Ruiqi Ge,Jie Wu

In this study, N-OSS combined with DDA was examined as an novel collector. The collecting performances and their synergetic interaction mechanisms were investigated comprehensively. The micro-flotation results illustrated N-OSS/DDA exhibited excellent selectivity on separation without pre-activation. Contact angle tests revealed N-OSS/DDA could selectively enhance the hydrophobicity of spodumene, whereas, they barely affected that of albite. FTIR, SEM-EDS and Zeta potential revealed N-OSS/DDA might co-adsorb on spodumene surface rather than that of albite. XPS analysis and DFT calculations inferred the O 1 2p orbitals of -COO- within N-OSS could bond with Al 3s orbitals and the -NH 3 + groups of DDA adsorbed onto spodumene surface via hydrogen bonding and electrostatic attraction. Meanwhile, the hydrogen bonding between N-OSS and DDA promoted the co-adsorption on spodumene surface. While N-OSS/DDA weakly adsorbed on albite surface through physical adsorption. N-OSS/DDA exhibited outstanding floatability, which maybe a good substitute for conventional collectors in future.

Novel Chemiluminescence Sensing for the Visual Detection of Sarcosine based Upon an Iron-based Metal–Organic Gel with Peroxidase-like Activity

Yuguang Jing,Yue Hu,Yongcheng He,Jiao Yang,Yingchun Li

Synthesis of N-acyl sarcosine from vegetable fatty acid as a novel collector and its low-temperature flotation performance and mechanism for apatite

Qing Jia,Wengang Liu,Wenbao Liu,Yonglun Wang,Zongxu Li

The flotation performance of traditional fatty acid collectors for apatite is significantly reduced under low-temperature conditions, limiting their industrial application. To overcome this limitation, a novel collector, N-fatty acyl sarcosine (N-FAS), was synthesized from vegetable fatty acid and evaluated for low-temperature flotation separation of fluorapatite from quartz and K-feldspar. Micro-flotation tests demonstrated that N-FAS exhibited superior selectivity, enhanced collecting power, and improved low-temperature tolerance compared to conventional sodium fatty acid (SFA). The adsorption mechanism was investigated using Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), revealing that N-FAS adsorbs onto the fluorapatite surface through synergistic coordination of both acyl and carboxyl groups with surface Ca²⁺ sites. In contrast, SFA adsorbs via a single carboxyl group interaction with Ca²⁺ sites. Total organic carbon (TOC) measurements confirmed that N-FAS adsorption on fluorapatite was approximately 40 % higher than SFA at low temperature. Molecular dynamics simulations (MDS) further demonstrated that at 10 ℃, N-acyl sarcosine exhibits lower interaction energy with water molecules and adopts a more compact spatial configuration in proximity to the fluorapatite surface compared to sodium fatty acid. These results indicate that N-FAS is a highly efficient low-temperature collector for apatite. In conclusion, this research provides a novel multidimensional interpretation of the N-FAS mechanism by bridging molecular-level MDS with macroscopic dynamic foam analysis, offering a scientific framework for the rational development of low-temperature collectors.

12-Plex UHPLC-MS/MS analysis of sarcosine in human urine using integrated principle of multiplex tags chemical isotope labeling and selective imprint enriching

Shi-En Chen,Jingwen Hu,Ping Yan,Jing Sun,Wenhui Jia,Shuyun Zhu,Xian-En Zhao,Huwei Liu

Urinary sarcosine was considered to be a potential biomarker for prostate cancer (Pca). In this work, an integrated strategy of multiplex tags chemical isotope labeling (MTCIL) combined with magnetic dispersive solid phase extraction (MDSPE), was proposed for specific extraction and high-throughput determination of sarcosine by ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). In the past three months, we have developed 8-plex MTCIL reagents with excellent qualitative and quantitative performance. In this work, the multiplexing capacity of MTCIL reagents (MTCIL360/361/362/363/364/365/366/375/376/378/379/381) was increased 1.5-fold from 8-plex to 12-plex. MTCIL359 was prepared and used to label sarcosine standard as internal standard (IS). The structural analogue derivative (MTCIL373-sarcosine) of all targeted MTCIL-sarcosine derivatives was synthesized and used as a novel dummy template to prepare dummy magnetic molecularly imprinted polymers (DMMIPs). The integration of MTCIL and DMMIPs procedures were extremely favorable to excellent chromatographic separation and efficient mass spectrometric detection. The labeling efficiency, chromatographic retention and mass spectrometry responses of MTCIL reagents were consistent for sarcosine. In a single UHPLC-MS/MS run (2.0 min), this method can simultaneously quantify sarcosine in 12-plex urine samples and achieve unbiased concentrations comparison between different urine samples. Analytical parameters including linearity (R 2 0.989–0.997), detection limits (0.02 nM), precision (2.6–11.5%), accuracy (96.1–107.4%), matrix effect, labeling and extraction efficiency were carefully validated. The proposed method was successfully applied for urinary sarcosine determination of healthy male individuals and Pca patients. It was found that the sarcosine concentrations in these two groups were statistically extremely significantly different ( P  < 0.001). The developed method was a powerful analytical tool to substantially promote the analysis throughput and large-scale experiments about the potential biomarker research.

The evaluation of N-OSS (N-oleoyl sarcosine) as an efficient collector in the flotation of spodumene from albite at low temperature: Experimental tests and atom-level calculation

Hui Shao,Bingqiao Yang,Huihua Luo,Huanyu Zhu,Ruiqi Ge,Jie Wu

In this study, N-OSS was explored as an efficient collector in the flotation of spodumene from albite. The flotation behaviors were examined by micro-flotation tests and the interaction mechanisms were comprehensively uncovered from atomical scale. It indicated N-OSS could effectively collect spodumene from albite at low temperature and N-OSS was more inclined to adsorb on spodumene surface than that on albite surface, thus significantly expanding the hydrophobicity difference. X-ray photoelectron spectroscopy (XPS) analysis suggested that N-OSS chemically interacted with both Ca and Al active sites on Ca-activated spodumene surface. Whereas, it weakly adsorbed on Ca-activated albite. Density functional theory (DFT) calculations reconfirmed that the adsorption of N-OSS on Ca-activated spodumene surface was more favorable than that on albite surface. The strong adsorption on spodumene was due to the hybridization reaction between the O 2p orbitals of C-O and C=O groups of N-OSS with Ca 4s orbitals as well as Al 3s orbitals on spodumene surface. Nevertheless, N-OSS weakly interacted with Ca atoms on Ca-activated albite due to steric hindrance. N-OSS exhibited selective collecting ability at low temperature in the flotation of spodumene from albite compared with conventional collector, which has promising prospects in the low-temperature flotation of spodumene from albite.

An intelligent ratiometric fluorescent assay based on MOF nanozyme-mediated tandem catalysis that guided by contrary logic circuit for highly sensitive sarcosine detection and smartphone-based portable sensing application

Jingyu Hou,Jun Wang,Jiawen Han,Juan Wang,Daiyong Chao,Qing Dong,Daoqing Fan,Shaojun Dong

As the well-known test-indicator for early prostate cancer (PCa), sarcosine (SA) is closely related to the differential pathological process, which makes its accurate determination increasingly significant. Herein, we for the first time expanded the peroxidase (POD)-like property of facile-synthesized Zn-TCPP(Fe) MOF to fluorescent substrates and exploited it to ratiometric fluorescent (RF) sensing. By harnessing the effective catalytic oxidation of MOF nanozyme toward two fluorescent substrates (Scopoletin, SC; Amplex Red, AR) with contrary changes, and target-responsive (SA + SOx)/MOF/(SC + AR) tandem catalytic reaction, we constructed the first MOF nanozyme-based RF sensor for the quantitative determination of SA. Superior to previous works, the operation of this RF sensor is under the guidance of AND-(ANDˆNAND) contrary logic circuit. The dual-channel binary output changes (from 1/0 to 0/1) not only enable the intelligent logical recognition of SA, bringing strengthened reliability and accuracy, but also manifest the proof-of-concept discrimination of PCa individuals and healthy ones. Through recording the fluorescence alterations of SC (F 465 ) and AR (F 585 ), two segments of linear relationships between ratiometric values (F 585 /F 465 ) and varied contents of SA are realized successfully. The LOD for SA could reach to as low as 39.98 nM, which outperforms all nanozyme-originated SA sensors reported till now. Moreover, this sensor also demonstrates high selectivity and satisfactory performance in human serum samples. Furthermore, the portable sensing of SA is realized under the assistance of smartphone-based RGB analysis, demonstrating the potential of point-of-care diagnostics of PCa in the future.