JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2022
·Vol. 46
·期 11
·e17133
·
IF 2.609
Qingyi Wei,Xiaomei Wang,Kaiqiang Wang,Hongbin Pu,Da-Wen Sun
Biofilms formed on food contact surfaces cause significant economic loss in the food sector and pose a serious public health concern. In this study, the formation of Shewanell a putrefaciens ( S. putrefaciens ) biofilms on nylon film at 30°C and 4°C was investigated. Results indicated that more biofilms were formed at 4°C, which peaked after 60 h incubation. The protease activity in biofilms was significantly higher than that in the corresponding plankton culture, and it was closely related to the development stage of biofilms. Besides, S. putrefaciens biofilms formed on nylon film in large yellow croaker juice and fresh cubes at 4°C were also investigated, and SDS-PAGE patterns demonstrated that S. putrefaciens biofilms showed a higher capacity to decompose proteins than planktonic bacteria. This work provided insight into the development of biofilms on nylon film, allowing for further controlling S. putrefaciens biofilms in food packages in the future. Practical applications Flexible plastic packaging with nylon film is widely used for packaging perishable food products including marine fish, smoked fish, processed meats such as sausage and bacon, dairy products, and prepared ready meals due to its various advantages of thermoformability, mechanical strength and toughness, transparency, and oxygen barrier properties. However, the formation of biofilms on the packaging surfaces can cause significant economic loss in the food industry and pose serious public health concerns. This study compared the formation of Shewanella putrefaciens biofilms on nylon film at 30°C and 4°C, and the results can provide further insight into the development of biofilms on nylon film, allowing for better control of S. putrefaciens biofilms in food packages, which is thus very beneficial for the food industry and consumers to ensure food safety.
研究领域:
食品科学
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微生物学
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生物技术
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产品关联
DOI
高影响因子
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
·
2024
·
IF 4.1
Liwen Zhang,Luyao Xi,Shuyu He,Huizi Wen,Shanyi Tan,Shaohua Chen
Bioremediation has garnered considerable interest due to its advantages of economy and no secondary pollution. However, the direct electron transfer rate between microorganisms and Cr(VI) is low. The bioreduction of Cr(VI) by Shewanella oneidensis MR-1 ( S. oneidensis ) in the presence of formylmethylflavin (FMF) was conducted to understand how FMF mediated the extracellular electron transfer process to improve Cr(VI) removal. In this study, FMF was firstly synthesized by modifing RF to increase its solubility and redox activity. The findings indicate that S. oneidensis /FMF exhibited better Cr(VI) removal performance compared to that of S. oneidensis . Under optimum conditions, 40 mg/L Cr(VI) could be completely removed by S. oneidensis /FMF within 120 h, while only 48.6% of Cr(VI) was removed by S. oneidensis , and the first order rate constant (k) for the Cr(VI) elimination by S. oneidensis /FMF (0.033 h −1 ) was about 4.1-fold greater than that of S. oneidensis (0.008 h −1 ). Moreover, the removal of Cr(VI) by S. oneidensis /FMF were dominated by reduction, and supplemented by adsorption and complexation. FMF enhance the extracellular electron transfer rate (EETR) was confirmed by electrochemical cyclic voltammetry (CV) and linear scanning voltammetry (LSV) experiments. This study emphasizes the potential important role of FMF in environmental bioremediation.
研究领域:
微生物学
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环境科学
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产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 26
·期 12
·12237-12246
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IF 2.914
Yang Mi,Shi Xianyang
Biosynthesized nanocomposites are attracting growing interests because they are environmentally friendly. Ag 2 S nanoparticles (Ag 2 S NPs) are deposited in situ on the surfaces of TiO 2 nanotubes (TNTs) via Shewanella oneidensis MR-1 to form Ag 2 S/TNT nanocomposites. The prepared Ag 2 S/TNTs nanocomposites are characterized using high-resolution transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and energy-dispersive X-ray spectroscopy. The results show that Ag 2 S NPs smaller than 8 nm are successfully synthesized and fabricated on the TNT surfaces with relatively uniform distribution. The catalytic performance of the Ag 2 S/TNT nanocomposites is evaluated for catalytic reduction in the presence of NaBH 4 and the photocatalytic degradation of 4-nitrophenol (4-NP) under visible light. The Ag 2 S/TNT nanocomposites show excellent catalytic activity and good stability in the 4-NP reduction process. The 4-NP degradation ratio reaches 98.3% in 50 min, and 87% conversion was achieved after eight cycles. The Ag 2 S/TNT nanocomposites also exhibit excellent photocatalytic activity for 4-NP at a rate of 0.69 h −1 , and the complete degradation of 4-NP was observed within 5 h. Therefore, this study offers an environmentally friendly approach to synthesize nanocomposites for practical applications.
研究领域:
环境科学
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纳米生物学
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催化化学
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DOI
PubMed
高影响因子
Small
·
2024
·Vol. 20
·期 33
·2400962
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IF 13
Han Chen,Yu-Qing Wei,Meng-Yuan Xu,Ma-Wei Zhu,Junying Liu,Yang-Chun Yong,Zhen Fang
Bioelectrochemical reactions using whole-cell biocatalysts are promising carbon-neutral approaches because of their easy operation, low cost, and sustainability. Bidirectional (outward or inward) electron transfer via exoelectrogens plays the main role in driving bioelectrochemical reactions. However, the low electron transfer efficiency seriously inhibits bioelectrochemical reaction kinetics. Here, a three dimensional and artificial nanoparticles-constituent inverse opal-indium tin oxide (IO-ITO) electrode is fabricated and employed to connect with exoelectrogens ( Shewanella loihica PV-4). The above electrode collected 128-fold higher cell density and exhibited a maximum current output approaching 1.5 mA cm −2 within 24 h at anode mode. By changing the IO-ITO electrode to cathode mode, the exoelectrogens exhibited the attractive ability of extracellular electron uptake to reduce fumarate and 16 times higher reverse current than the commercial carbon electrode. Notably, Fe-containing oxide nanoparticles are biologically synthesized at both sides of the outer cell membrane and probably contributed to direct electron transfer with the transmembrane c -type cytochromes. Owing to the efficient electron exchange via artificial and biosynthetic nanoparticles, bioelectrochemical CO 2 reduction is also realized at the cathode. This work not only explored the possibility of augmenting bidirectional electron transfer but also provided a new strategy to boost bioelectrochemical reactions by introducing biohybrid nanoparticles.
研究领域:
微生物学
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纳米技术
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环境科学
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DOI
PubMed
高影响因子
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
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2013
·
IF 3.848
Jie Yuan,Yiwen Chen,Guangqi Zhou,Haijiang Chen,Haichun Gao
Background Bacteria adopt a variety of lifestyles in their natural habitats and can alternate among different lifestyles in response to environmental changes. At high cell densities, bacteria can form extracellular matrix encased cell population on submerged tangible surfaces (biofilms), or at the air–liquid interface (pellicles). Compared to biofilm, pellicle lifestyle allows for better oxygen access, but is metabolically more costly to maintain. Further understanding of pellicle formation and environmental cues that influence cellular choices between these lifestyles will definitely improve our appreciation of bacterial interaction with their environments. Methods Shewanella oneidensis cells were cultured in 24-well plates with supplementation of varied divalent cations, and pellicles formed under such conditions were evaluated. Mutants defective in respiration of divalent cations were used to further characterize and confirm unique impacts of iron. Results and conclusions Small amount of Fe 2 + was essential for pellicle formation, but presence of over-abundant iron (0.3 mM Fe 2 + or Fe 3 + ) led to pellicle disassociation without impairing growth. Such impacts were found due to S. oneidensis -mediated formation of insoluble alternative electron acceptors (i.e., Fe 3 O 4 ) under physiologically relevant conditions. Furthermore, we demonstrated that cells preferred a lifestyle of forming biofilm and respiring on such insoluble electron acceptors under tested conditions, even to living in pellicles. General significance Our finding suggests that bacterial lifestyle choice involves balanced evaluation of multiple aspects of environmental conditions, and yet-to-be-characterized signaling mechanism is very likely underlying such processes.
研究领域:
微生物学
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环境科学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2024
·
IF 8.1
Haoran Tian,Qingyu Wu,Qiuju Wu,Chengyou Sun,Ruimei Zhang,Jie Wei,Haiyin Xu,Zhiming Liu,Chao Huang,Ping Wang
Nano zero-valent iron (nZVI) has been widely employed for the elimination of heavy metals from wastewater. However, the practical application of nZVI is greatly limited due to its fast aggregation and surface passivation. In this study, a carbon nanotube-supported nZVI composite (nZVI@CNTs) was synthesized and coupled with Shewanella oneidensis MR-1 to enhance the removal of Cr(VI) from aqueous media. The results demonstrated that the coupling of nZVI@CNTs and MR-1 (nZVI@CNTs/MR-1) exhibited superior performance in eliminating Cr(VI) compared to using nZVI@CNTs or MR-1 alone. Electrochemical characterization demonstrated that CNTs acting as electron shuttles improve the electron transfer from MR-1 to nZVI, thus facilitating the passivation layer dissolution. XRD analysis proved that MR-1 can effectively promote the dissolution of the Fe(III) passivation layer with the existence of CNTs. FTIR and XPS analyses confirmed that CNTs contain many functional groups (such as C − C and C = O) related to extracellular electron transfer, thus promoting the electron transfer of MR-1 to nZVI. Three-dimensional excitation-emission matrix (3D-EEM) analysis showed that the extracellular polymeric substances (EPS) generated from MR-1 participated in the Cr(VI) elimination. The mechanism analysis demonstrated the Fe(II) dissolved from the nZVI passivation layer reduces Cr(VI) to Cr(III) and then removes it by co-precipitation, and the EPS secreted by MR-1 also participates in the Cr(VI) adsorption and reduction. Generally, the coupling of nZVI@CNTs and MR-1 successfully addresses the drawbacks of nZVI and demonstrates a remarkable ability to remove Cr(VI).
研究领域:
环境科学
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微生物学
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产品关联
DOI
高影响因子
Surfaces and Interfaces
·
2023
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IF 6.2
Diandi Li,Haijun He,Zhongxuan Xu,Hong Deng
Coexistence of chromium and iron, manganese and copper in industries is common, which may affect the microbial treating efficiency and toxicity to microorganisms, thus restricting the application of microbial treatment technology. In this research, Cu 2+ promoted the reduction of Cr(VI) and the fixation of total chromium in bimetallic systems for both Shewanella oneidensis MR-1 and Bacillus subtilis , while Mn 2+ and Fe 3+ exhibited inhibition effects. Generally, groups with coexisting heavy metal of 0.1 mM were most affected, no matter promotive or inhibitive. Toxicity analysis revealed that high concentration of heavy metal ions led to damage to the cell membranes and reduction of their survival rate. To clarify the optimal condition for microbial treatment, response surface methodology was used to investigate the influencing factors of Cr(VI) reduction by S. oneidensis . The total chromium concentration in the supernatant was correlated with three factors, and the significant order of influence was: culture time > culture temperature > pH.
研究领域:
微生物学
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环境科学
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DOI
高影响因子
IET Nanobiotechnology
·
2018
·Vol. 12
·期 4
·441-445
·
IF 2.059
Xiaojie Song,Xianyang Shi,Mi Yang
Biosynthesised nanocomposites have attracted growing interests attributed to their ‘green’ synthesis nature in recent years. Shewanella oneidensis MR-1, a dissimilatory metal-reducing bacterium, was used to reduce palladium (II) nitrate to palladium (0) nanoparticles (Pd NPs) under anaerobic conditions, resulting in the in situ formation of Pd NPs immobilised on TiO 2 nanotubes (TNTs) (Pd/TNTs nanocomposites). The Pd/TNTs nanocomposites were characterised by transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, energy dispersive X-ray, and electron spin resonance, respectively. The results indicated that Pd NPs are successfully grown on the TNTs without aggregation. Photocatalytic degradation of methylene blue (MB) by Pd/TNTs nanocomposites under simulated sunlight was also investigated. Pd/TNTs nanocomposites had photocatalytic efficiency superior to that of single TiO 2 nanomaterials. The photocatalytic activity of Pd/TNTs nanocomposites can be enhanced by S. oneidensis MR-1. The results showed that after only 10 min, the degradation ratio of MB reached 98.7% by Pd/TNTs nanocomposites when simultaneously assisted with S. oneidensis MR-1.
研究领域:
环境科学
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材料科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Jingxin Ye,Yunfang Qian,Weiqing Lan,Jing Xie
Shewanella putrefaciens and Listeria monocytogenes are concerned by scientists and consumers due to their ability to produce dense biofilms to contaminate and cause food spoilage. The object of this study was to investigate the antimicrobial and anti-biofilm activities of octyl gallate (OG) against the two food-related bacteria. Results showed that the antimicrobial activities of OG were in a dose-dependent manner and the minimum inhibitory concentration (MIC) of S. putrefaciens and L. monocytogenes were 0.2 mmol/L and 0.1 mmol/L, respectively. OG can rapidly penetrate the bacteria and produce reactive oxygen species (ROS). Then, OG and ROS can lead to membrane damage and cell rupture, causing DNA and protein leakage. Our data also strongly suggested that OG not only can inhibit biofilms formation, but also eradicate the performed biofilms. Furthermore, it was shown that OG, as an additive, has great potential in antimicrobial application to prolong the shelf life of large yellow croakers. These prove that OG can combat bacteria and eradicate biofilm against S. putrefaciens and L. monocytogenes .
研究领域:
食品科学
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微生物学
·
食品安全
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产品关联
DOI
高影响因子
ULTRASONICS SONOCHEMISTRY
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2021
·
IF 7.491
Okon Johnson Esua,Jun-Hu Cheng,Da-Wen Sun
A novel method of thermoultrasound-assisted plasma functionalized buffer (PFB) for decontaminating grass carp was evaluated using the Box–Behnken design (BBD) with processing variables including PFB generating voltage (P V ), ultrasound treatment time (U T ) and temperature (T P ). The predicted models were found to be significant ( p < 0.05) and displayed sufficient fitness with experimental data as indicated by non-significant ( p > 0.05) lack of fit and high coefficient of determination (R 2 ≥ 0.97) values. The optimum decontamination conditions for the responses of S. putrefaciens and S. Typhimurium were P V of 66 V, U T of 14.90 min and T P of 60 ℃, achieving reductions of 4.40 and 3.97 log CFU/g, respectively, with a desirability of 0.998. Among the variables, temperature presented higher significance for inactivating bacteria and the production of volatile basic nitrogen and lipid peroxidation under the optimized conditions were within the limits of freshness for grass carp. Additionally, the effects of PFB and the optimized thermoultrasound-assisted PFB decontamination were mild on the microstructure of grass carp with slight ruptures and loose myofibril structures, indicating the potential of thermoultrasound-assisted PFB for seafood products decontamination with reduced processing time.
研究领域:
食品科学
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微生物学
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生物技术
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Mingxia Jiang,Yuyin Lin,Liyuan Xu,Zhihang Chen,Junxian Wu,Yong Yuan,Lihua Zhou
Extracellular respiratory bacteria (ERB) assume a significant role in biological and environmental remediation, so the rapid detection of ERB is essential to monitor its biological repair process. This study developed a microfluidic colorimetric biosensor for rapid and sensitive detection of ERB model strain Shewanella oneidensis MR-1. In the process of separation and enrichment of bacteria based on immune magnetic nanoparticles (MNPs), the biological signal is significantly amplified by the excellent peroxidase activity of flower-like CoOOH (FL-CoOOH). Firstly, immune MNPs, samples and immune FL-CoOOH were mixed and incubated within the microfluidic chip’s channel. Magnetic capture in the separation chamber yielded MNP-bacteria-CoOOH complex. Then, 3, 3′, 5, 5′-tetramethylbenzidine (TMB) substrate was added to make the FL-CoOOH nanozyme catalyze the formation of yellow product. Finally, the target bacteria in the microplate were quantitatively detected. Based on the excellent simulated enzyme activity and stability of FL-CoOOH, the detection can be completed within 45 min after the introduction of the microfluidic chip. The detection was 5×10 1 ∼5×10 6 CFU/mL. Compared with the previously reported colorimetric sensors for MR-1, the sensitivity was increased by 99.54 % to 23 CFU/mL. This study uniquely integrates optical sensors and microfluidic technology for rapid ERB detection with enhanced portability. The design of biosensor can facilitate further investigations into extracellular respiration mechanisms and rapid ecological environment restoration applications.
研究领域:
生物技术
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环境科学
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分析化学
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微流控
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
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IF 16.744
Cholnam Ri,Fengxiang Li,Hyokchol Mun,Linan Liu,Jingchun Tang
The technology of integrating sulfidated zero-valent iron and functional anaerobic bacteria has been extensively explored to improve the removal efficiency of pollutants and enhance the performance of anaerobic microbial system. In this study, the simultaneous DCNB(2,4-dichloronitrobenzene) reduction and methane production by ball-milled 1:1 Fe 0 /FeS 2 (briefly, 1:1 Fe 0 /FeS 2 ), Shewanella oneidensis MR-1 and microbial consortium (MC) were firstly investigated. The result showed that the DCNB reduction was increased by 2.6 and 1.7 times in 1:1 Fe 0 /FeS 2 + MR-1 group compared to 1:1 Fe 0 /FeS 2 and MR-1, respectively, while it further increased by almost 2 times in 1:1 Fe 0 /FeS 2 + MC + MR-1 group as compared to that of 1:1 Fe 0 /FeS 2 + MR-1 group. Moreover, the methane production was drastically facilitated via the reciprocal interaction among FeS as the reaction product, MR-1 and MC. Dechlorination was not found, mainly due to the inability of 1:1 Fe 0 /FeS 2 and the inexistence of dechlorinating bacteria in original inoculum. Meanwhile, the microbial community was also changed, with genera like Eubacterium capable of reducing nitroaromatic compound and Methanosarcina as acetoclastic methanogen enriched by 1:1 Fe 0 /FeS 2 and MR-1. Therefore, fast DCNB reduction and methane production using 1:1 Fe 0 /FeS 2 in anaerobic microbial system, gives useful reference for both establishment of pollutant removal and biogas production. In conclusion, Fe 0 /FeS 2 , as a kind of sulfidated ZVI, is a good candidate for enhancing both pollutant degradation and CH 4 production in anaerobic wastewater treatment.
研究领域:
环境工程
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微生物学
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废水处理
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）