ADVANCED FUNCTIONAL MATERIALS
·
2023
·
IF 19
Qiyue Wang,Tao Wang,Shengfei Yang,Hui Du,Bo Zhang,Sirui Cui,Daishun Ling,Fangyuan Li
Calcium ions (Ca 2+ ) play crucial roles as second messengers in living cells and are associated with the onset and development of chronic brain diseases, including epilepsy. Dynamic and real-time imaging of extracellular calcium levels ([Ca 2+ ] ex ) in the brain holds great potential for identifying disease foci. Magnetic resonance imaging (MRI) is a powerful non-invasive tool for Ca 2+ imaging with unlimited depth penetration. However, current available Ca 2+ MR sensors lack the sensitivity to monitor small [Ca 2+ ] ex alterations associated with chronic brain diseases in vivo. In this study, a highly sensitive Ca 2+ -dependent T2-T1 switchable magnetic nanosensor is reported, consisting of Ca 2+ -specific ligands engineered near-paramagnetic iron oxide nanoparticles. Surface ligand modifications can be adjusted to regulate the interaction energy between sensors and Ca 2+ , allowing for customizable response range and sensitivity to fulfill the in vivo imaging requirements. The optimized sensors enable non-invasive visualization of early-stage seizure focus in mice undergoing epilepsy.
研究领域:
神经科学
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生物医学工程
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医学成像
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纳米技术
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 11
·1688-1698
·
IF 4.776
Pei Liu,Hongyi Zheng,Zhe Yang,Li Ba,Wei Zhu,Leping Lin,Yuxuan Xiong,Zushun Xu,Jinghua Ren
Imaging-guided photodynamic/photothermal (PDT/PTT) synergetic therapy is important in more precise and efficient cancer treatment. Herein, gadolinium-chelated porphyrin–protein composite based on gold nanorods (GNRs@BPP-Gd) was synthesized through a straightforward and environmentally friendly method. Based on the high longitudinal relaxivity (9.544 mM−1 s−1), GNRs@BPP-Gd can serve as a decent T1-weighted magnetic resonance imaging (MRI) probe to steer treatment. A relevant cellular uptake assay showed that GNRs@BPP-Gd could be internalized into cancer cells effectively with negligible cytotoxicity. Furthermore, MRI-guided PDT/PTT studies in vivo indicated that GNRs@BPP-Gd could efficiently take effect after intravenous injection, leading to almost complete ablation at the tumor site. A single treatment was conducted for comparison with the combined treatment, indicating that synergetic therapy greatly enhanced the anti-tumor effect.
研究领域:
生物医学工程
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纳米技术
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癌症研究
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分子影像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2022
·
IF 6.14
Yinghua Tao,Yucheng Li,Dandan Wei,Min Liang,Pengfei Ren,Jidong Dai,Tianzhu Zhang,Jiandu Lei,Peidang Liu
Multifunctional nanomaterials with diagnostic and therapeutic capabilities offer promising customized nanomedicines for cancers. However, achieving good execution of these functions in vivo employing a single nanoframework remains challenging, and only a few of these magnetic nanocarriers have been validated for clinical use. In this study, based on biocompatibility and remarkable multifunctionalities, we describe a novel approach to synthesize small Fe3O4 nanoparticles conjugated with natural polysaccharide pectin and assembled into a pH-responsive T1-weighted magnetic resonance imaging contrast agent, Fe3O4@PD, for better chemotherapeutic efficacy. In vitro studies revealed that the conjugated doxorubicin (DOX) was quickly released in the tumor microenvironment by cleavage of the acid-sensitive acylhydrazone bond at a release rate of 89.72% and exerted a considerable inhibitory effect on breast cancer cells. In vivoT1-weighted images of mice showed that Fe3O4@PD displayed darkening to brightening contrast enhancement at tumor sites, and the relative signal-to-noise ratio of the tumor site reached its maximum (increased to about 2.27-fold) for distinguishing the normal and tumor tissues. Thus, this Fe3O4@PD-based system has the potential to be a multifunctional nanodrug delivery system, and as a smart theragnostic platform, it has broad application prospects in clinical and biological works, such as cell-based diagnostics and in vivo medication monitoring.
研究领域:
纳米技术
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生物医学工程
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药学
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癌症研究
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磁共振成像
✓ 已验证
DOI
高影响因子
OPTICS EXPRESS
·
2025
·Vol. 33
·期 11
·22185-22195
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IF 3.3
Lu Li,Feng Qin,Zhiguo Zhang,Zhiguo Zhang,Tao Jia
The determination of the energy of the lowest triplet excited state (ET1) for metalloporphyrin photosensitizers, a key parameter for rationalizing energy transfer processes in metalloporphyrins, facilitates a better understanding and optimization of their functionality. In this study, a strategy was proposed for determining the ET1 values in metalloporphyrin systems by analyzing the temperature dependence of the oxygen quenching rate constant using time-resolved spectroscopy, with Platinum(II) octaethylporphyrin (PtOEP) and Palladium(II) octaethylporphyrin (PdOEP) as representative examples. Through a thermal equilibrium approximation of the excited triplet state (T1) and the excited state of oxygen, a theoretical model was introduced in which the oxygen quenching rate exhibits a nonlinear dependence on temperature, enabling the determination of ET1 values. Additionally, the validity of this approach was demonstrated through excitation spectra of metalloporphyrins. Our work provides an effective pathway and offers new insights into the energy-loss mechanisms involved in related photoconversion processes.
研究领域:
物理化学
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光化学
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光谱学
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材料科学
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分子物理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2018
·Vol. 6
·期 8
·2209-2218
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IF 5.831
Siqi Wang,Wenzhi Ren,Jianhua Wang,Zhenqi Jiang,Madiha Saeed,Lili Zhang,Aiguo Li,Aiguo Wu
At present, transmembrane glycoprotein CD133 highly expressed pancreatic cancer stem cells (PCSCs), with the features of chemotherapeutic/radiotherapeutic resistance and exclusive tumorigenic potential, are considered as the primary cause of metastasis and recurrence in pancreatic cancer, and therefore are an effective target in the disease treatment. Furthermore, with the launch of precision medicine, multifunctional nanoprobes have been applied as an efficient strategy for the magnetic resonance imaging (MRI)-guided photothermal therapy (PTT) of pancreatic cancer. In this research, with the aim of achieving precise MRI-guided PTT in CD133 highly expressed PCSCs, novel bTiO2-Gd-CD133mAb nanoprobes were designed and successfully prepared by loading Gd-DOTA and CD133 monoclonal antibodies on black TiO2 nanoparticles. It was very interesting to find that the r1 relaxivity value of the nanoprobes was 34.394 mM−1 s−1, about 7.5 times that of commercial Magnevist (4.5624 mM−1 s−1), which indicates that the nanoprobes have good potential as MRI T1 contrast agents with excellent performance. Herein, CD133 highly expressed PANC-1 cells were selected and verified as PCSCs model. In vitro experiments demonstrated that the nanoprobes exhibited active-targeting ability in PANC-1 cells, and consequently could specially enhance T1-weighted MR imaging and 808 nm near-infrared (NIR)-triggered PTT efficiency in the PCSCs model. Our study not only provides a new strategy for the effective treatment of pancreatic cancer and its’ stem cells, but also further broadens the application of black TiO2 in the field of cancer theranostics.
研究领域:
癌症研究
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纳米技术
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医学成像
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诊疗一体化
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 33
·18647-18655
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IF 2.936
Xi Zhou,Xiaolin Lv,Wen Zhao,Tiantian Zhou,Shupeng Zhang,Zhan Shi,Shefang Ye,Lei Ren,Zhiwei Chen
This study reports a multifunctional core/shell nanoparticle (NP) that can be used for amplified magnetic resonance image (MRI), enhanced photothermal therapy (PTT) and magnetic hyperthermia therapy (MHT) due to its surface coating with a porous shell. Importantly, by means of introducing the surface coating of a porous shell, it helps entrap large quantities of water around NPs and allow more efficient water exchange, leading to greatly improved MR contrast signals. Besides, the porous shell helps the near-infrared (NIR) absorbance of the core, and then the extremely enhanced thermal effect can be obtained under synergistic combination of PTT and MHT. By synthesizing multifunctional porous MnFe2O4/PB as an example, we found that the transversal relaxivity (r2) of MnFe2O4 NPs might improve from 112.11 to 123.46 mM−1 s−1, and the specific absorption rate (SAR) of MnFe2O4/PB nanoparticles reached unprecedented levels of up to 4800 W g−1 compared with the SAR 1182 W g−1 of PTT under an 808 nm laser and 180 W g−1 of MHT under an external AC magnetic field. Meanwhile, when MnFe2O4 was decorated on PB nanoparticles, the magnetic properties became lower slightly, but the synergistic photothermal/magnetic hyperthermia conversion was enhanced greatly. Subsequently, in vitro T1–T2 dual-modal MRI, PTT and MHT results verified that MnFe2O4/PB could serve as an excellent MRI/PTT/MHT theranostic agent. Furthermore, the MnFe2O4/PB NPs were applied as a T1–T2 dual-modal MRI, PTT and MHT theranostic agent for in vivo MRI-guided photothermal and magnetic hyperthermia ablation of tumors by intratumoral injection in 4T1 tumor-bearing mice. The T1–T2 dual-modal MR imaging result shows a significantly contrast in the tumor site. The MPB-mediated PTT and MHT result shows high therapeutic efficiency as a result of high photothermal and magnetic hyperthermia conversion efficiency. The multifunctional NPs have a great potential application for future clinical tumorous diagnosis and treatment.
研究领域:
纳米技术
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生物医学工程
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材料科学
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癌症治疗
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医学影像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2023
·
IF 2.7
Yike Hou,Shuqi Huang,Zhe Tang,Zhangsen Yu,Muhammad Zubair Iqbal,Xiangdong Kong
Photo-responsive combinational therapeutics attained significant attention in cancer theranostic owing to the versatile features of nanomaterials. The simplest nano-design with unique properties to carry out multifunctionalties is a challenging assignment. Herein, a facile solvothermal method is employed to fabricate homogeneous MnO 2 sea urchin-shaped nanoparticles (MnO 2 -OVA NPs) with a high loading capacity (86.15%) of ovalbumin (OVA) antigen. The prepared MnO 2 -OVA NPs demonstrated excellent biocompatibility against different cell lines and significant cellular uptake, resulting in mature dendritic cells to enhance anti-tumor immunity. The synthesized sea urchin-shaped MnO 2 -OVA NPs further exhibited self-generated catalytic properties that efficiently relieve tumor hypoxia and improve cancer therapies. More excitingly, MnO 2 -OVA NPs endorsed reactive oxygen species (ROS) in response of minute UV irradiation (mW) for photodynamic therapy. Further, the prepared photo-responsive nanoparticles showed promising T 1 -MR imaging and relaxivity properties for diagnosis and real-time drug delivery monitoring. These synergetic therapeutic strategies produced by MnO 2 -OVA NPs showed excellency as a potential theranostic agent.
研究领域:
纳米技术
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癌症研究
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免疫治疗
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医学成像
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诊疗一体化
✓ 已验证
产品关联
DOI
高影响因子
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2017
·Vol. 83
·期 23
·
IF 3.807
Cui-Wei Chu,Bin Liu,Na Li,Shi-Gang Yao,Dan Cheng,Jia-Dong Zhao,Ji-Guo Qiu,Xin Yan,Qin He,Jian He
Thiobencarb is a thiocarbamate herbicide used in rice paddies worldwide. Microbial degradation plays a crucial role in the dissipation of thiobencarb in the environment. However, the physiological and genetic mechanisms underlying thiobencarb degradation remain unknown. In this study, a novel thiobencarb degradation pathway was proposed in Acidovorax sp. strain T1. Thiobencarb was oxidized and cleaved at the C—S bond, generating diethylcarbamothioic S-acid and 4-chlorobenzaldehyde (4CDA). 4CDA was then oxidized to 4-chlorobenzoic acid (4CBA) and hydrolytically dechlorinated to 4-hydroxybenzoic acid (4HBA). The identification of catabolic genes suggested further hydroxylation to protocatechuic acid (PCA) and finally degradation through the protocatechuate 4,5-dioxygenase pathway. A novel two-component monooxygenase system identified in the strain, TmoAB, was responsible for the initial catabolic reaction. TmoA shared 28 to 32% identity with the oxygenase components of pyrimidine monooxygenase from Agrobacterium fabrum, alkanesulfonate monooxygenase from Pseudomonas savastanoi, and dibenzothiophene monooxygenase from Rhodococcus sp. TmoB shared 25 to 37% identity with reported flavin reductases and oxidized NADH but not NADPH. TmoAB is a flavin mononucleotide (FMN)-dependent monooxygenase and catalyzed the C—S bond cleavage of thiobencarb. Introduction of tmoAB into cells of the thiobencarb degradation-deficient mutant T1m restored its ability to degrade and utilize thiobencarb. A dehydrogenase gene, tmoC, was located 7,129 bp downstream of tmoAB, and its transcription was clearly induced by thiobencarb. The purified TmoC catalyzed the dehydrogenation of 4CDA to 4CBA using NAD+ as a cofactor. A gene cluster responsible for the complete 4CBA metabolic pathway was also cloned, and its involvement in thiobencarb degradation was preliminarily verified by transcriptional analysis.IMPORTANCE Microbial degradation is the main factor in thiobencarb dissipation in soil. In previous studies, thiobencarb was degraded initially via N-deethylation, sulfoxidation, hydroxylation, and dechlorination. However, enzymes and genes involved in the microbial degradation of thiobencarb have not been studied. This study revealed a new thiobencarb degradation pathway in Acidovorax sp. strain T1 and identified a novel two-component FMN-dependent monooxygenase system, TmoAB. Under TmoAB-mediated catalysis, thiobencarb was cleaved at the C—S bond, producing diethylcarbamothioic S-acid and 4CDA. Furthermore, the downstream degradation pathway of thiobencarb was proposed. Our study provides the physiological, biochemical, and genetic foundation of thiobencarb degradation in this microorganism.
研究领域:
环境微生物学
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分子生物学
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生物化学
✓ 已验证
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Fangnan Xiao,Yue Xiao,Fangman Chen,Xiaolin Liu,Chentao Lin,Jianxin Chen,Yunkun Wu
Highly stable and multifunctional fluorescent quantum dots are particularly attractive in practical applications. Here, a new kind of ultra-small-sized silicon quantum dot-gadolinium (SiQD-Gd) was successfully fabricated by a newly-designed facile hydrothermal growth and chelating method. The obtained SiQD-Gd exhibited outstanding water dispersibility, stability and good fluorescent property with the quantum yield of 11.6%. SiQD-Gd displayed a low cytotoxicity in normal cell lines (HELF, HEK293F) and tumor cell lines (H1299, A549). Meanwhile, SiQD-Gd showed excellent magnetic resonance response with r 1 relaxation rate of 10.5 mmol L −1 ·s −1 and r 2 relaxation rate of 47.5 mmol L −1 ·s −1 , which are 2.5 and 7.4 times enhanced comparing to that of the commercial MR agent Magnevist. In vivo studies showed significant contrast enhancement effect of its T 1 - and T 2 -weighted MR imaging. In addition, in vivo fluorescent imaging for mice and zebrafish indicated its potential applications in fluorescent tracking. Thus, the excellent multimodal imaging capacity and biocompatibility of SiQD-Gd make it a potential imaging agent for clinic applications.
研究领域:
材料科学
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纳米技术
·
医学成像
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2019
·
IF 0
Ying Liu,Yuxia Tang,Ying Tian,Jiang Wu,Jing Sun,Zhaogang Teng,Shouju Wang,Guangming Lu
Hydroxyapatite nanoparticles have drawn much attention in the development of multifunctional nanosystems because of their excellent biocompatibility. In the report, gadolinium-doped hydroxyapatite nanorods (NHA:Gd) were controlled synthesized through adjusting the amount of gadolinium. The NHA:Gd showed a high loading capacity for doxorubicin (118 μg per milligram) and a pH-responsive drug release ability. The r1 value of obtained doxorubicin (DOX)-loaded NHA:Gd (NHA:Gd-DOX) solution reached 0.0472 (μg/mL)−1 s–1 and was higher than that of commercial T1 contrast Gd-DTPA (0.0314 (μg/mL)−1 s–1). In vivo experiments demonstrated that NHA:Gd-DOX exhibited excellent MR imaging property. The results of therapeutic efficacy demonstrate that the tumor volume treated by NHA:Gd-DOX was significantly reduced compared to that treated by free DOX. In vivo toxicity experiments demonstrated no obvious damage to the major organs in NHA:Gd-DOX treated mice. Considering the excellent biocompatibility, T1WI contrast ability, and enhanced therapeutic efficacy, these NHA:Gd-DOX have great potential for applications as MR T1 contrast agents and drug carriers for cancer therapy.
研究领域:
纳米技术
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医学成像
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癌症治疗
·
材料科学
·
药学
✓ 已验证
产品关联
DOI
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Asim Mushtaq,Han Zhang,Mingyue Cui,Xiaoqing Lin,Shuqi Huang,Zhe Tang,Yike Hou,M. Zubair Iqbal,Xiangdong Kong
Magnetite hydroxyapatite nanoparticles show notable multifunctional therapeutic features in nanomedicine . However, designing MHAp nanoparticles with optimum dimensions, good stability and biocompatibility for specific biomedical applications is still in its infancy. Herein, a facile approach was employed to prepare ROS-responsive chlorin e6 and silk fibroin-loaded ultrathin magnetic hydroxyapatite nanorods for T 1 -magnetic resonance imaging and photodynamic therapy. Specifically, the Fe 3 O 4 -HAp nanorods were synthesized by the hydrothermal method , and then Pluronic® F-127 was used to improve the aqueous dispersion, biocompatibility, and loading capacity. Moreover, silk fibroin protein was encapsulated by a tri-block copolymer with good loading efficiency. Finally, the theranostic nano-assembly MHNRs-SF-Ce6 was completed by the addition of Ce6. The prepared MHNRs demonstrated excellent biocompatibility, cellular uptake, and ROS generation ability in in vitro after 660 nm laser irradiation, leading to the apoptosis of 4T1 mouse breast cancer cells. Notably, the designed MHNRs exhibited excellent r 1 relaxivity value and T 1 magnetic resonance imaging in vitro due to their minimal width (2 nm) of nanorods. Therefore, the obtained MHNRs-SF-Ce6 self-assembled nanoparticles provide an effective strategy for cancer diagnosis and photodynamic therapy.
研究领域:
纳米医学
·
生物材料
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2018
·
IF 1.712
Xiaoqiang Jia,Yun He,Lei Huang,Dawei Jiang,Wenyu Lu
The high-molecular weight polycyclic aromatic hydrocarbons (PAHs) pyrene and typical long chain alkane n -hexadecane are both difficult to degrade. In this study, n -hexadecane and pyrene degrading strain Rhodococcus sp. T1 was isolated from oil contaminated soil. Strain T1 could remove 90.81% n -hexadecane (2 vol%) and 42.79% pyrene (200 mg·L − 1 ) as a single carbon within 5 days, respectively. Comparatively, the degradation of pyrene increased to 60.63%, but the degradation of n -hexadecane decreased to 87.55% when these compounds were mixed. Additionally, identification and analysis of degradation metabolites of Rhodococcus sp. T1 in the above experiments showed that there were significant changes in alanine, methylamine, citric acid and heptadecanoic acid between sole and dual substrate degradation. The optimal conditions for degradation were then determined based on analysis of the pH, salinity, additional nutrient sources and liquid surface activity. Under the optimal conditions of pH 7.0, 35 °C, 0.5% NaCl, 5 mg·L − 1 of yeast extract and 90 mg·L − 1 of surfactant, the degradation increased in single or dual carbon sources. To our knowledge, this is the first study to discuss metabolite changes in Rhodococcus sp. T1 using sole substrate and dual substrate to enhance the long-chain alkanes and PAHs degradation potential.
研究领域:
环境微生物学
·
生物修复
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）