JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2021
·
IF 4.464
Shanshan Wei,Haolin Xiao,Miao Gu,Zhencheng Chen,Liangli Cao
In this study, a sensitive and quantitative label-free electrochemical immunosensor based on bimetallic [email protected] nanoparticles , Prussian blue (PB), and reduced graphene oxide-tetraethylene pentamine nanocomposite (rGO-TEPA) was constructed to achieve sensitive detection of HBsAg. A one-pot green method was used to synthesize interlocked rGO-TEPA/PB hybrid composites as high-performance materials with good conductivity . PB was the electron transfer mediator used to provide electrochemical signals. By utilizing rGO-TEPA as supporting material , the stability and intensity of PB were greatly improved. The incorporation of highly conductive [email protected] nanoparticles could further amplify the electrochemical signal, and the high adsorption capacity could provide an efficient substrate for hepatitis B surface antibody (HBsAb) immobilization. Under optimal conditions, the immunoassay had a wide linear range from 0.25 pg mL −1 to 400 ng mL −1 and a low detection limit of 0.08 pg mL −1 (S/N = 3). This method was also applied to the analysis of human serum samples, and the obtained results were on par with those of electrochemiluminescence immunoassay (ECLIA), suggesting significant potential for this new method in clinical research.
研究领域:
生物医学工程
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分析化学
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临床诊断
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2018
·
IF 3.235
Shuang Zhao,Yuchan Zhang,Songtao Ding,Jingchuan Fan,Zhi Luo,Kunguo Liu,Qi Shi,Weichi Liu,Guangchao Zang
A reliable and non-invasive electrochemical immunosensor for nuclear matrix protein 22 (NMP22) was presented based on reduced graphene oxide-tetraethylenepentamine (rGO-TEPA) and gold nanoparticles‑platinum nanoparticles-metal organic frameworks (AuNPs-PtNPs-MOFs) nanomaterial . rGO-TEPA is a promising template to enhance the loading ability of electrode for its large specific surface area and abundance in amino groups. AuNPs-PtNPs-MOFs nanomaterial was synthesized by NaBH 4 reduction method and applied to immobilize biomolecules due to their good biocompatibility, well conductivity , and excellent H 2 O 2 electrocatalytic ability. The electrochemical response signal was primarily originated from the obstruction of immobilized NMP22 antigen towards electrocatalytic reduction of H 2 O 2 and the signal was recorded by differential pulse voltammetry . Under optimum conditions, the immunosensor showed a sensitive response towards NMP22 at a broad concentration range from 0.005 ng·mL −1 to 20 ng·mL −1 . Two strong linear ranges could be observed at 0.005 ng·mL −1 to 0.5 ng·mL −1 (R 2 = 0.9833), and 0.5 ng·mL −1 to 20 ng·mL −1 (R 2 = 0.9909), with a low LOD of 1.7 pg·mL −1 (S/N = 3). Satisfactory reproducibility, stability and selectivity were obtained for NMP22 detection in real urine samples compared with standard ELISA assay, demonstrating the good analytical performance and potential non-invasive clinical application of this label-free electrochemical immunosensor.
研究领域:
分析化学
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生物传感器
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纳米技术
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医学诊断
✓ 已验证
产品关联
DOI
高影响因子
GREEN CHEMISTRY
·
2023
·Vol. 25
·期 15
·5999-6008
·
IF 9.8
Yuanyuan Zhang,Wanxiang Zhang,Yan Wang,Shuhang Ren,Yucui Hou,Weize Wu
To reduce the emission of NO from fossil fuel combustion and the energy consumption associated with NH3 production, the development of simple and efficient methods to transform NO into high-value-added NH3 is essential. Here, we report the efficient catalytic hydrogenation of NO to NH3 under mild reaction conditions by the synergistic catalysis of functionalized ionic liquids (FILs) and Pd catalysts. In this system, an FIL [TEPA][Im] acted as both an absorbent and a catalyst. The presence of [TEPA][Im] enhanced the catalytic performance by changing the coordination and electronic environment of Pd on the Pd/C catalyst. First, NO was absorbed by [TEPA][Im]; then, the hydrogenation of absorbed NO to NH3 was performed at a low temperature via the synergistic catalyzation of [TEPA][Im] and Pd/C. The effects of various factors on NO hydrogenation to NH3 were also investigated. Remarkably, Pd/C exhibited outstanding properties in the formation of NH3 with a high yield of 67% in the FIL [TEPA][Im] under the conditions of 100 °C and an H2 pressure of 0.5 MPa. [TEPA][Im] could be reused multiple times and the product NH3 could be recovered by flashing.
研究领域:
环境化学
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催化
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可持续能源
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
·
IF 5.4
Weidong Wang,Ying Gao,Meijie Sun,Zhiqiang Xu,Yanan Tu
Coal gasification fine slag (CGFS) is a solid waste produced during the gasification process, and its high carbon content limits its large-scale utilization. However, the preparation of amine modified hierarchical porous carbon adsorbents (HPCs) from CGFS not only reduces the production costs of matrix materials but also achieves high-value utilization. In this study, the high-carbon component of CGFS was utilized as the raw material to successfully synthesize an amine-modified HPCs for coupled CO 2 capture and mineralization via pre-impurity removal, chemical activation, and amine impregnation. Results demonstrated that HPCs exhibited excellent CO₂ adsorption performance. Specifically, the CO₂ adsorption capacities of 20 % PEI@HPC and 10 % TEPA@HPC with optimal amine loading reached 2.11 mmol/g and 1.62 mmol/g at 298 K, respectively. Both demonstrated outstanding CO₂ adsorption-desorption cycling performance and stability after 10 cycles. Furthermore, the amine-modified HPCs effectively enhanced the carbonation efficiency of desilicated slag, with 20 % PEI@HPC and 30 % TEPA@HPC increasing the mineralization efficiency of the desilicated slag from 28.68 % to 75.01 % and 82.69 %, respectively. Finally, this study elucidated the mechanism of coupled CO₂ capture and mineralization with amine-modified HPCs. The adsorption isotherm models revealed that CO₂ adsorption on the amine-modified HPCs was influenced not only by physical adsorption but also through chemical adsorption dominated by amine functional groups. In this study, a cost-effective CGFS was used as the raw material to synthesize amine-modified HPCs with excellent CO 2 adsorption performance. This approach was expected to address the issues of single-source and expensive matrix materials and had some potential for industrial- scale utilization.
研究领域:
环境科学
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材料科学
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碳捕集与封存
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废弃物高值化利用
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吸附技术
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 10
·1-10
·
IF 6.232
Cao Liangli,Xiao Haolin,Fang Cheng,Zhao Feijun,Chen Zhencheng
Using gold and magnetic nanoparticles co-decorated reduced graphene oxide-tetraethylenepentamine (rGO-TEPA/Au-MNPs) as the magnetic platform for capturing the primary antibody (Ab 1 ), separation and preconcentration of immunocomplex, a novel homogeneous electrochemical immunosensor was successfully developed. The newly prepared magnetic rGO-TEPA/Au-MNPs, compared with MNPs, exhibited better stability and enhanced electrical conductivity attributed to rGO-TEPA, and showed higher biorecognition efficiency due to AuNPs. In addition, Au@PtNPs were prepared and modified with secondary antibody (Ab 2 ) as an efficient signal probe for signal readout. Using carcinoembryonic antigen (CEA) as a model analyte, the prepared immunosensor demonstrated satisfactory properties like high stability, good repeatability and selectivity, wide linear range (5.0 pg mL −1 ~200.0 ng mL −1 ) as well as low detection limit (1.42 pg mL −1 ). The homogenous electrochemical immunosensor was applied to the detection of CEA in human serum and was found to exhibit good correlation with the reference method. Thus, the proposed rGO-TEPA/Au-MNPs-based homogenous immunoassay platform might open up a new way for biomarker diagnosis.
Graphical Abstract
研究领域:
分析化学
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生物传感器
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纳米技术
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生物医学工程
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癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
HaiyanYang,Xinzhong Wang,Jun Liu,Wanzhen Liu,Yue Gong,Yankun Sun
Solid amine materials are promising adsorbents for CO 2 capture from biogas , flue gas and other similar gas mixture. Here, tetraethylenepentamine (TEPA)-impregnated porous polymeric resin (ADS-17) with very high CO 2 adsorption capacity was successfully prepared. Its CO 2 separation performance from simulated biogas was investigated by breakthrough experiments. And the influence of parameters such as TEPA loading, adsorption temperature and flow rate on the adsorption behaviours were studied in detail. The optimal TEPA loading was 40 wt% (ADS-17–40%TEPA) and the CO 2 adsorption capacity of this adsorbent reached the optimal value (ca. 264.2 mg▪g −1 ) at the flow rate of 200 mL▪min −1 . The CO 2 adsorption capacity of ADS-17–40%TEPA had no pronounced change from 25 °C to 70 °C, but it decreased obviously when the adsorbent was heated to 90 °C. At 100 °C, the adsorption capacity became negligible. According to detailed characterization, it was inferred that the pores of ADS-17–40%TEPA in the range of 13–115 nm were probably responsible for CO 2 diffusion. In addition, ADS-17–40%TEPA possessed good reversibility and the CO 2 adsorption capacity was nearly stable after two adsorption–desorption cycles. ADS-17–40%TEPA with high and fast CO 2 adsorption exhibits a very promising application in CO 2 separation from biogas.
研究领域:
化学工程
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环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2025
·
IF 4.3
Wenhao Liu,Qingcai Chen,Tengfei Xu,Sitong Liu,Yifan Sun,Huadong Huang
Low-cost support materials and simple synthesis methods can significantly reduce the cost of solid amine technology, which is crucial for its commercialization. This research work utilized blast furnace slag as raw material and adopted a green, simple treatment method to prepare the support materials (BFSC), which exhibited high specific surface area (238.83 m 2 /g) and pore volume (1.09 cm 3 /g). The main component of the support material is magnesium aluminum metasilicate. The support material was modified with different contents of polyethyleneimine (PEI) and tetraethylenepentamine (TEPA), the results showed that BFSC-50 %TEPA exhibited the best CO 2 adsorption performance (3.27 mmol/g). Subsequently, the effects of adsorption conditions on BFSC-50 %TEPA were investigated, including temperature, humidity, and different gas compositions. In atmosphere condition of 15 % CO 2 and 85 % air (with 60 % humidity), high temperatures promote the formation of a urea derivative, which significantly impairs the material’s CO 2 adsorption capacity. Finally, the cyclic stability of BFSC-50 %TEPA was tested.
研究领域:
环境科学
·
化学工程
·
材料科学
·
碳捕集与封存
✓ 已验证
产品关联
DOI
高影响因子
Electrocatalysis
·
2020
·Vol. 11
·期 5
·513-521
·
IF 2.587
Xiao Haolin,Wei Shanshan,Chen Zhencheng,Cao Liangli
Herein we report a label-free amperometric immunosensor based on reduced graphene oxide-tetraethylene pentamine (rGO-TEPA) hybrid material co-modified by gold and iron-oxide magnetic nanoparticles (Au/MNPs-rGO-TEPA) for detection of carcinoembryonic antigen (CEA). The Au/MNPs-rGO-TEPA nanomaterials were prepared and coated on the working electrode as a sensing platform for antibody (anti-CEA) immobilization and signal amplification. This nanomaterial exhibited some excellent functionalities, such as magnetic and enhanced electrical properties due to its large specific surface area and favorable conductivity. Thionine (Thi) molecule was attached onto rGO-TEPA via π-π stacking and was used as an electrochemically active substance. The immunosensor was developed based on the increase in quantity of CEA captured, proportional to the decrease in peak currents of Thi. Under optimal conditions, the Au/MNPs-rGO-TEPA/Thi-based immunosensor showed impressive linear range from 0.1 pg/mL to 150 ng/mL. In addition, the proposed method had excellent linearity, high sensitivity, and relatively good stability, thereby offering a good platform for early clinical analysis.
A novel label-free binary nanoparticle-decorated reduced graphene oxide-tetraethylene pentamine-based electrochemical immunosensor was developed. Au/MNPs-rGO-TEPA/Thi was used as electrochemical sensitive platform for quantitative detection of CEA.
研究领域:
生物传感器
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纳米技术
·
分析化学
·
癌症诊断
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Haolin Xiao,Shanshan Wei,Miao Gu,Zhencheng Chen,Liangli Cao
Alpha-fetoprotein (AFP) plays an important role as a marker in the clinical diagnosis of cancer. However, the sensitive detection of AFP in clinical samples is still challenging. Therefore, we developed a highly sensitive electrochemical immunosensor for the effective detection of AFP. In this study, thionine (Thi) and gold nanoparticles (AuNPs) co-modified reduced graphene oxide-tetraethylene pentamine (rGO-TEPA-Thi-Au), which was used as the immunosensor platform to fix the primary antibody (Ab 1 ), leading to a large improvement in the sensitivity of the immunosensor. Furthermore, ordered mesoporous carbon (CMK-3) modified with AuPt binary nanoparticles ( [email protected] ) was used as the signal probe for further signal amplification because CMK-3 can provide a large specific surface area for in-situ reduction of AuPtNPs with good conductivity. Therefore, a dual signal amplification was observed in the amperometric i-t curve, and the detection limit of the proposed immunosensor was effectively enhanced. The constructed immunosensor showed a wide linear range of 0.005 to 100 ng mL −1 and a low detection limit of 0.0022 ng mL −1 at S/N = 3. Furthermore, the proposed immunosensor exhibited excellent stability and recovery, demonstrating its potential for clinical applications.
研究领域:
生物传感器
·
癌症诊断
·
纳米技术
·
电化学
·
分析化学
·
材料科学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Shengzhong Rong,Lina Zou,Ye Zhu,Ze Zhang,Hongfeng Liu,Yingcong Zhang,Hui Zhang,Hongmin Gao,Hongjun Guan,Jing Dong,Yupeng Guo,Fenghai Liu,Xiaoxia Li,Hongzhi Pan,Dong Chang
Nuclear matrix protein 22 (NMP22) is a crucial bladder cancer marker, the accurate detection of which is conductive to improving the effectiveness of tumor prevention and treatment. However, due to low sensitivity, it is impossible for conventional immunoassay to detect the trace of NMP22 at the early stages of tumor progression. It is crucial to develop a simple, sensitive and accurate detection method of NMP22. As a variety of two-dimensional (2D) material, reduced graphene oxide-tetraethylene pentamine (rGO-TEPA) demonstrates excellent conductivity and contains abundant amino groups. As a sort of 3D material, silica-modified Cu-based metalorganic frameworks deposited silver nanoparticles ( [email protected] 2 @AgNPs) composites are characterized by large surface area, excellent biocompatibility and water stability, which allows the immobilization of anti-NMP22 in large amounts via Ag-N bonds. Through a combination of the advantages exhibited by 2D material, 3D material, AgNPs and disposable screen-printed electrode, a disposable and sensitive label-free electrochemical immunosensor has been successfully assembled for NMP22 detection. In addition to exhibiting a wide linear range (0.1 pg∙mL −1 -1000 ng∙mL −1 ) and a low detection limit (33.33 fg∙mL −1 , S/N = 3), the proposed immunosensor also demonstrated satisfactory selectivity, strong reproducibility and high stability. Moreover, the excellent performance in detecting human urine samples makes it applicable in practice.
研究领域:
生物医学工程
·
分析化学
·
癌症研究
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF POROUS MATERIALS
·
2021
·Vol. 29
·期 1
·19-31
·
IF 2.496
Ge Lin,Wei Jianwen,Geng Linlin,Chen Siqi,Liao Lei
An amine-modified ZSM-5/SBA-16 (ZS) micro-/mesoporous composite was synthesized for CO 2 capture using nonionic tri-block copolymer pluronic (F127) as a template, tetraethyl orthosilicate (TEOS) as a silicon source and ZSM-5 zeolite as part of silicon aluminum source by embedding method. The amine-bifunctionalized samples were prepared by first grafting with aminepropyltriethoxysilane (APTES) and then impregnating with different concentrations of tetraethylenepentamine (TEPA). The amine efficiency, adsorption kinetics, thermal stability, regeneration performance and the effects of impregnated amine loadings (30–60%) and temperatures (30–90 °C) on the CO 2 adsorption performance were investigated using a thermal gravimetric analyzer (TGA) in the mixed gases (15 vol% CO 2 and the balance of N 2 ). The best adsorbent after modification of ZSM-5/SBA-16 composite material was screened to study CO 2 adsorption mechanism and adsorption kinetics. With the increase of the TEPA amount, the adsorption capacity of amine-bifunctionalized material first increased and then decreased. At 90 °C, the bifunctionalized ZSM-5/SBA-16 displayed the highest CO 2 adsorption performance of 1.630 mmol/g at the TEPA loading of 55%. The CO 2 adsorption mechanism of the bifunctionalized material was the synergistic effect of physical adsorption and chemical adsorption. After five adsorption/desorption cycle regenerations, the saturated adsorption capacity of the composite material was 1.496 mmol/g, which was only 8.2% lower than the original adsorption capacity. The composites exhibited good CO 2 adsorption performance, indicating the promising industrial applications for CO 2 capture form actual flue gas after desulfurization.
研究领域:
材料科学
·
环境工程
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Water Process Engineering
·
2022
·
IF 7.34
Zongying Zhang,Qinze Liu,Tingting Gao,Congde Qiao,Jinshui Yao,Changbin Zhang
Alkaline lignin, a by-product of the paper-pulping process, can undergo phenolation by pyrogallol in the presence of acetone and sulfuric acid to prepare phenolated-alkaline-lignin (PAL). PAL can be further reacted with tetraethylenepentamine (TEPA) to prepare lignin-based catecholamine (PAL-TEPA), which exhibits excellent ability to remove hazardous Cr (VI) from simulated wastewater. In this study, PAL and PAL-TEPA were systematically investigated by SEM, FT-IR, 1 H NMR, TGA, and XPS. The conditions for the preparation of PAL and the various variables affecting the adsorption behavior of PAL-TEPA were optimized sequentially. The adsorption process followed the pseudo-second-order model and fitted to the Langmuir isotherm equation. The maximum adsorption amount reached 600 mg g −1 ( R = 75 %) under optimized conditions (pH = 2.0, C 0 = 200 mg L −1 , V = 100 mL, T = 30 °C, dos. = 25 mg, t = 24 h). Toxic Cr (VI) ions were successfully converted to harmless Cr (III) ions and removed by PAL-TEPA adsorbent. This approach opens new avenues for the utilization of lignin and new kinds of adsorbent for Cr (VI) removal.
研究领域:
环境科学
·
化学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）