ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2026
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IF 11.3
Jia Zhang,Shuang Zhang,Xinxin Xu,Hua Kuang,Chuanlai Xu,Xiaoling Wu
Inflammatory bowel disease (IBD) is a widespread public health problem worldwide, and environmental pollutants are regarded as the cause of IBD. Tween 80 (P80) may trigger IBD; however, its underlying mechanism of action has not been fully clarified, especially with respect to diet-induced susceptibility. This study systematically investigated the effect of P80 on the IBD development in male mice on a normal diet and high-fat diet (HFD) at an acceptable daily intake level. After 6 weeks of oral exposure to P80 (0.1, 1, 6%), the high-dose group of mice exhibited a significant increase in the disease activity index, a significant body weight loss, and a severe inflammatory response, with HFD markedly augmenting these effects. Multiomics results indicated that P80 exposure disrupted intestinal tryptophan metabolism and significantly reduced indole-3-acetic acid (IAA) levels. In vivo intestinal inflammation was transcriptionally regulated by genes related to the AhR/NF-κB/NLRP3 signaling pathway. Supplementing with IAA can improve inflammatory effects. In vitro studies confirmed that P80 exposure inhibited Lactobacillus from producing IAA, leading to an increase in the level of intestinal inflammation. This study revealed the specific toxic mechanism of P80-induced IBD and found that dietary factors have a synergistic toxic effect, providing new insights into the health risk assessment of environmental pollutants.
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PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Shiyu Bao,Likun Zhao,Yuanwen Liu,Lewei Yang,Peng Liu,Chaoqi Chen,Xuhui Mao
Polycyclic aromatic hydrocarbons (PAHs) are a group of contaminants with low bioavailability and biodegradability . As a result, removing PAHs from contaminated soils is often inefficient. In this study, soils spiked with fluoranthene and pyrene were inoculated with Aeromonas sp. BCP-3, a newly isolated strain from coke-plant sludge , and treated in slurry bioreactors . The removal of PAHs was significantly affected by aeration rate, slurry water-soil mass ratio, stirring rate, temperature, and the concentration of Tween-80. Such effects were attributed to changes in BCP-3 growth, PAHs solid-liquid mass transfer, and slurry particle size. Notably, under optimal conditions (i.e., aeration rate of 1.5 L/min, temperature of 30 °C, stirring rate of 200 rpm, water-soil mass ratio of 1.5:1, and Tween-80 concentration of 1.3 %), 96.3 % and 97.2 % of initially spiked fluoranthene and pyrene were removed within 12 days, respectively. A PAH-contaminated soil was collected from a local coking plant and treated under the same conditions, except for the Tween-80 concentration. For the real soil, the Tween-80 concentration was 4 % to ensure a significant proportion of PAHs released into the liquid phase. Twenty-one days after inoculation of BCP-3, the removal efficiencies of low molecular weight PAHs and high molecular weight PAHs were 82.2 % and 51.8 %, respectively. The removal efficiencies of PAHs were significantly higher than the slurry without BCP-3 or HgCl 2 -sterilized slurry. BCP-3 improved the bioavailability and biodegradability of PAHs, resulting in effective PAH removal in slurry bioreactors . Overall, successful bioaugmentation was achieved with the addition of Tween-80 and the optimization of operating conditions.
研究领域:
环境科学
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微生物学
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高影响因子
ENERGY & FUELS
·
2023
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IF 5.3
Zhihui Ling,Shang Wang,Zhongzhan Leng,Chengmei Li,Wenhui Zhang,Xinxin Kang,Xianghui Qi,Cunsheng Zhang
To improve the performance of ethanol production from food waste, a consolidation process involving both the addition of surfactant and cell immobilization was investigated. Results showed that the optimum glucose concentration was 200 g/L for ethanol fermentation using Saccharomyces cerevisiae as inoculum. With the addition of 0.5% Tween 80, the ethanol production was improved from 68.1 to 79.8 g/L, which was attributed to the increased mass transfer of cells provoked by Tween 80. In a cell-immobilized system, wheat straw was used as the matrix to carry the cells. A high ethanol production of 87.8 g/L was obtained with the sequential fermentation mode due to the high cell density on the cell carrier. The results of a mass balance calculation revealed that the ethanol production of food waste achieved 340.4 kg/t (on a dry basis), which provided an insight that food waste was a suitable substrate for ethanol production. The main cost was spent on energy consumption in the processes of food waste sterilization, hydrolysis, fermentation, and ethanol distillation, and measures for reducing the energy were suggested to improve the economic performance of ethanol production.
研究领域:
生物技术
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环境科学
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发酵工程
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高影响因子
FOOD HYDROCOLLOIDS
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2024
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IF 11
Shuang Ma,Mengyao Liu,Sanmei Long,Kaiyan Xu,Ke Luo,Ying Xu
Short-chain glucan (SCG) recrystallization has emerged as an effective approach for producing resistant starch microparticle (RSP), which hold promise in both food science and clinical nutrition. This study introduces a novel method utilizing Tween 80 (TW80) to mediate the interfacial recrystallization of SCG into A-type crystalline RSP (ARSP) through acetone precipitation. TW80 plays a crucial role by stabilizing water-in-oil microemulsion droplets within acetone-oil mixtures, facilitating the formation of uniform and spherical ARSP ranging in size from 0.2 μm to 2 μm. The surface characteristics of ARSP, including porosity and smoothness, are notably influenced by SCG concentration. As SCG concentration increases, the melting temperature of ARSP rises from 117 °C to 145 °C, accompanied by a decrease in average surface area from 12.07 to 8.35 m 2 /g, alongside an increase in crystallinity. Moreover, the in vitro digestion test revealed that the digestion rate of ARSP was decreased from 0.73 h −1 to 0.39 h −1 with increasing SCG concentration, while increasing the resistant starch (RS) content in ARSP from 48.6% to 70.5%. This enhancement offers a strategic approach for modulating the digestion rate of ARSP, presenting opportunities for tailored release profiles and improved stability in food science applications. These findings underscore the potential of ARSP as a functional ingredient with significant implications for nutritional and clinical settings.
研究领域:
食品科学
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临床营养学
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高影响因子
FOOD HYDROCOLLOIDS
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2021
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IF 9.147
Weiyi Zhang,Junmeng Lu,Xue Zhao,Xinglian Xu
The structures of proteins on the interface of oil-in-water emulsions that influence the interfacial film strength are significant to the emulsions stabilization. It is expected that the interfacial protein could be fully recovered based on the competitive displacement of low-molecular surfactant, when the recovery approach and relative parameters are optimized, so that the structural properties of interfacial proteins can be evaluated to clarify their adsorption behaviour and film forming mechanism. In the present study, an optimized and efficient approach to fully recovering interfacial myofibrillar protein (MP) was established by optimizing protein concentration, Tween-20 concentration and different accelerating treatments (agitation or freeze-thawing), for interface-induced structural changes analysis. It was found that the interfacial MP in 2.5 mg/ml protein-stabilized emulsion could be fully recovered in 96 h with 4% (v/v) Tween-20 under agitation treatment. Freeze-thawing treatment could accelerate the desorption process by which interfacial MP in the above emulsion could be fully recovered in 3 cycles with 2% or 3% (v/v) Tween-20 but might cause damage to the MP structure. Based on the established recovery approach, it was concluded that the tertiary structure of interfacial MP unfolded to expose hydrophobic residue branch for interfacial adsorption and film formation, which further caused aggregation of desorbed MP through hydrophobic interaction. While the α-helix decreased due to interface-induced denaturation and the β-sheet increased to improve protein-protein interaction on the interface. These results provided an optimized strategy to analyze interfacial protein structure.
研究领域:
食品科学
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蛋白质化学
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高影响因子
Scientific Reports
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2016
·Vol. 6
·期 1
·1-8
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IF 5.228
Deng Ling-Li,Taxipalati Maierhaba,Que Fei,Zhang Hui
Attempts were made to solubilize thymol in Tween 80 micelle to study the solubilization mechanism of thymol and the effect of solubilization on its antioxidant activity. The maximum solubilized concentration of thymol in a 2.0% (w/v) Tween 80 micelle solution is 0.2 wt%. There was no significant difference in Z-average diameter between the empty micelles and thymol solubilized micelles. 1 H NMR spectra indicated that 3-H and 4-H on the benzene ring of thymol interacted with the ester group between the hydrophilic head group and the hydrophobic tail group of Tween 80 by Van der Waals’ force. Ferric reducing antioxidant potential (FRAP) and cupric ion reducing antioxidant capacity (CUPRAC) assays showed that the reducing antioxidant activity of free thymol did not change after solubilized in Tween 80 micelles. Compared to free thymol, the solubilized thymol showed higher activities to scavenge DPPH (2,2-diphenyl-1-picrylhydrazyl) and hydroxyl radicals. The present study suggested a possible preparation of thymol-carrying micelles with enhanced antioxidant activities that could be applied in food beverages.
研究领域:
食品科学
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化学
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生物化学
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PubMed
高影响因子
Asia-Pacific Journal of Chemical Engineering
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2016
·Vol. 11
·期 5
·673-682
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IF 0.728
Rui Li,Zhaoliang Wu,Yanji Wang,Linlin Ding,Wei Liu
Prevention of irreversible aggregation of whey soy proteins (WSP) in their foam fractionation from soy whey wastewater is important for achieving their large-scale recovery. For effectively preventing the irreversible aggregation of WSP, this work studied its dependencies on the WSP enrichment ratio and the height of the collected foam which freely collapsed. The results show that most insoluble aggregates formed in the desorption of WSP from the gas–liquid interface, and their formation was closely related to their high local enrichment in the foam. Based on the above results, a chemical defoaming agent with 25% Tween 20 was developed to reduce the relative content of insoluble aggregates in the foamate and the decrease reached about 80%, compared to the case without Tween 20. The prevented irreversible aggregation effectively improved food-related properties of the WSP product. Copyright © 2016 Curtin University of Technology and John Wiley & Sons, Ltd.
研究领域:
食品科学
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蛋白质工程
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高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2018
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IF 4.513
Hui-Bo Qin,Yi-Le Du,Yu Zhang,Xiao-Qin Wang,Zhen-Feng Sun,Chang-Yu Sun,Guang-Jin Chen,Lan-Ying Yang,Zhi Li
The toxicity of hydrate inhibitors used in the field has attracted more attention. A natural material, i.e. , whey protein, was assessed to act as kinetic hydrate inhibitors (KHIs) in a sapphire cell and an autoclave equipped with particle video microscope (PVM) and focused beam reflectance measurement (FBRM) probes. The pressure dropping method was utilized to estimate the hydrate induction time, and the results demonstrate that the hydrate inhibition performance of whey protein is superior to that of the commercial KHI, polyvinylpyrrolidone. The induction time for the pure water system was found to increase linearly with the increase in the dosage of whey protein until it reached approximately 2.5 wt%. As it is usually inevasible to apply KHIs under conditions of high water contents , the feasibility of whey protein as a KHI in the (oil + water) system with and without an emulsifier of Tween 20 was evaluated. A small amount of oil was found to have no obvious influence on the induction time for the system with whey protein under the circumstances without the addition of emulsifier. When Tween 20 was added, the induction time obviously increased under the same operating conditions, which could be attributed to the emulsifier–protein interactions in aqueous solutions. The oil drops decrease and are distributed more evenly with the increasing dosage of Tween 20 under the PVM and FBRM probes measurement. Additionally, the particles decrease during the hydrate formation process. This work presents a natural KHI and gives the inhibition discipline in a high-water-content system.
研究领域:
化学工程
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环境科学
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材料科学
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高影响因子
Scientific Reports
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2021
·Vol. 11
·期 1
·1-11
·
IF 4.38
Yang Zuoyi,Mao Xingchen,Cui Jiahao,Wang Yujie,Zhang Yaping
This study examines the specific effect of Tween 80 on the conversion of anthracene (ANT) in laccase medium system regarding surfactant chemical changes and mechanism. The conversion rate and degradation products of ANT were investigated in different concentrations of Tween 80 solution. Between Tween 80 concentration 0–40 critical micelle concentrations (CMC), the kinetic parameter-k (h −1 ) and corresponding half-life T 1/2 decreased with increasing concentration. When Tween 80 was above 20 CMC the laccase-medium system converted > 95% of ANT to anthraquinone within 12 h. During the entire enzymatic reaction, the laccase activity in the system increased with increasing Tween 80 concentration. Combined with GC/MS analysis of the product, it was speculated that hydrogens belonging to the ether-oxygen bond and carbon–carbon double bond α-CH of Tween 80, were removed by the laccase-media system, promoting its degradation. Additionally, enhanced activity caused by oxygen free radicals (ROS) such as RO• and ROO•, continuously oxidized Tween 80, which in turn produced free radicals while converting ANT. This study provides new theoretical support toward the application of surfactants in the elimination of polycyclic aromatic hydrocarbons.
研究领域:
环境科学
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生物化学
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PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
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2022
·Vol. 34
·期 1
·1-15
·
IF 4.727
Wu Chuang,Wang Haixiang,Cao Jin
Electrospinning is a promising technique for preparing bioartificial blood vessels. Nanofibers prepared by electrospinning can simulate the structure of extracellular matrix to promote cell adhesion and proliferation. However, thorn-like protrusions can appear as defects on electrospun scaffolds and coaxial electrospun nanofibers often have no clear core/shell structure, which can seriously affect the quality of bioartificial blood vessels. To address these problems, Tween 80 is added to the electrospinning solution, which results in a stable Taylor cone, eliminates the thorn-like protrusions on electrospun bioartificial blood vessels, and reduces interfacial effects due to different core/shell solutions during coaxial electrospinning. Simulations, biomechanical tests, and in vivo studies were performed. The results demonstrate the excellent mechanical properties and biocompatibility of the bioartificial blood vessel. This research provides a useful reference for optimizing the electrospinning process for fabricating bioartificial blood vessels. Graphical Abstract
研究领域:
组织工程学
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生物材料科学
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生物医学工程
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PubMed
高影响因子
INTERNATIONAL MICROBIOLOGY
·
2024
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IF 2.3
Zhang Mingle,He Zhimin,Xu Xiaoyi,Ji Fan,Wang Bin
This study investigates the advantages of combined microbial degradation of polycyclic aromatic hydrocarbons (PAHs) in reducing the inhibitory effects of high-concentration eluents commonly used in soil washing. A microbial synergistic strategy was proposed using Arthrobacter sp. SZ-3 and Pseudomonas putida B6-2 as the key bacteria in the presence of Tween 80. The results show that in systems with Tween 80, the SZ-3 strain exhibits a strong capacity to degrade three types of PAH compounds, while the B6-2 strain follows multiple degradation pathways. Mixed bacteria achieved degradation rates 60.70% higher than single bacteria at varying concentrations of Tween 80. Additionally, the average growth rates of mixed bacteria increased by 1.17–1.37 times, aligning with the changes in the functional group. Protein activity detection within each degradation system corresponded with growth quantity and the cyclic variation characteristics of ETS enzyme activity. Notably, the ETS activity of mixed bacteria was 150% higher than that of single bacteria. At a Tween 80 concentration of 500 mg/L, the degradation rates of PAHs (Phe, Flu, Pyr) by mixed bacteria were significantly higher than those by single bacteria. The catechol 1,2-dioxygenase activity of mixed bacteria was 2.30 times higher than that of single bacteria. While Tween 80 did not alter the PAH degradation pathways, it significantly influenced the accumulation amount and duration of the characteristic intermediate product. This provides a reference for the remediation of recalcitrant pollutants under conditions involving high-concentration surfactants.
研究领域:
环境微生物学
·
污染修复
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DOI
PubMed
高影响因子
BIOMASS & BIOENERGY
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2020
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IF 3.551
Wei Yang,Huan Fan,Min Zhou,Zheng Zhou,Lishi Yan,Xin Ju,Liangzhi Li
Stable cellulases are critical for in situ hydrolysis of ionic liquid-pretreated lignocellulose , and surfactants were reported to improve the stability of cellulase in practical circumstances. In this study, synergistic effect of Tween-80 and [Emim]OAc was first identified to enhance the hydrolysis of lignocellulose by Paenibacillus sp. LLZ1 cellulase, proving the effectiveness of surfactants in in situ hydrolysis. Protein fluorescence and near-UV circular dichroism spectroscopy analyses illustrated that ionic liquids might move local residues and alter global tertiary structure of cellulase, leading to higher instability. The addition of Tween-80 might turn the microenvironment of cellulase residues more hydrophilic and reduce the contact of protein with deactivation factors. The synergistic system was further applied in the in situ hydrolysis of microcrystalline cellulose and bagasse cellulose by Paenibacillus cellulase. The enzymatic hydrolysis rate of microcrystalline cellulose was enhanced by 2.5-fold and the final conversion was improved by 45%. Scanning electron microscopy demonstrated the substrate solubilizing effect during reaction by monitoring the morphological changes of lignocellulosic substrates . This study highlighted the potential of applying synergistic reaction system in in situ enzymatic hydrolysis of lignocellulose, and revealed the inherent mechanism initially.
研究领域:
生物化学
·
可再生资源与环境科学
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高影响因子
危险品化学品经营许可证（带存储）