FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Shuang Ma,Mengyao Liu,Sanmei Long,Kaiyan Xu,Ke Luo,Ying Xu
Short-chain glucan (SCG) recrystallization has emerged as an effective approach for producing resistant starch microparticle (RSP), which hold promise in both food science and clinical nutrition. This study introduces a novel method utilizing Tween 80 (TW80) to mediate the interfacial recrystallization of SCG into A-type crystalline RSP (ARSP) through acetone precipitation. TW80 plays a crucial role by stabilizing water-in-oil microemulsion droplets within acetone-oil mixtures, facilitating the formation of uniform and spherical ARSP ranging in size from 0.2 μm to 2 μm. The surface characteristics of ARSP, including porosity and smoothness, are notably influenced by SCG concentration. As SCG concentration increases, the melting temperature of ARSP rises from 117 °C to 145 °C, accompanied by a decrease in average surface area from 12.07 to 8.35 m 2 /g, alongside an increase in crystallinity. Moreover, the in vitro digestion test revealed that the digestion rate of ARSP was decreased from 0.73 h −1 to 0.39 h −1 with increasing SCG concentration, while increasing the resistant starch (RS) content in ARSP from 48.6% to 70.5%. This enhancement offers a strategic approach for modulating the digestion rate of ARSP, presenting opportunities for tailored release profiles and improved stability in food science applications. These findings underscore the potential of ARSP as a functional ingredient with significant implications for nutritional and clinical settings.
研究领域:
食品科学
·
临床营养学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Ruijuan Wang,Chunliu Ma,Haitao Yan,Hailiang Zhao,Pu Wang,Shoubing Zhang,Jianwei Ju,Shuyan Yu,Zhigang Yin
Co-encapsulated hydrophilic vitamin C (Vc) and hydrophobic xanthoxylin (GX-50) liposomes (L-Vc-GX-50) were prepared using ethanol injection and ultrasonic methods and characterized by dynamical light scattering, ζ-potential, cryogenic transmission electron microscopy, UV–vis spectroscopy, Fourier transform infrared spectroscopy, antioxidant capacity, tyrosinase activity, and in vitro permeation measurements. L-Vc-GX-50 are formed by the assembly of egg yolk lecithin, cholesterol, and tween 80, with Vc and GX-50 encapsulated in their hydrophilic and hydrophobic cavities, respectively. By considering the hydrodynamic radius (Rh), ζ-potential, and encapsulation efficiency (EE) of L-Vc-GX-50, the optimum formulation was determined to be egg yolk lecithin/cholesterol/tween 80/GX-50 or Vc (15:3:3:1, w/w) with phosphate buffer (pH 6.8, 0.01 M). The as-prepared L-Vc-GX-50 have an average Rh of 114 nm, a negative ζ-potential (−23.63 mV), a high EE of Vc (73.3%) and GX-50 (87.4%), and a unilamellar vesicle structure. Compared co-encapsulated L-Vc-GX-50 with single encapsulated Vc or GX-50 liposomes (L-Vc or L-GX-50), the EE of Vc in L-Vc-GX-50 is obviously higher than that of L-Vc, while the EE of GX-50 in L-Vc-GX-50 is similar to that in L-GX-50; the antioxidant capacity and tyrosinase inhibition activity of L-Vc-GX-50 are higher than those of L-GX-50 or L-Vc. The in vitro permeation data indicate that the final cumulative permeation amount of Vc and GX-50 in L-Vc-GX-50 can reach around 2.1 and 1.3 mg/cm2, respectively. The release kinetics fitting data show that the release kinetics of L-Vc-GX-50 follows the Korsmeyer–Peppas model and that the release of active ingredients is mainly dominated by the synergistic effect of diffusion release and rupture release. Moreover, L-Vc-GX-50 has good low-temperature storage stability, and the storage stability of GX-50 in L-Vc-GX-50 is significantly improved compared to L-GX-50. The main objective of this study is to improve the instability of Vc and the insolubility of GX-50 in cosmetics.
研究领域:
药剂学
·
纳米技术
·
化妆品
·
药物递送系统
·
分析化学
·
生物物理学
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Tao Yang,Chang Liu,Yan Zheng,Tristan C. Liu,Kexin Li,Jianguo Liu,Yaowei Liu,Peng Zhou
Conversion of major ginsenosides into minor ginsenosides improves its pharmacological effects. This study managed to convert major ginsenosides into minor ginsenoside Rg3 and compound K (CK) by optimizing acidolysis conditions (pH, incubation temperatures and time) and evaluated the effects of the whey protein isolate (WPI)/Tween 80 ratio on the physicochemical properties of ginsenoside nanoemulsions . The optimal conditions for acidolysis are incubation at 110 °C for 3 h at a pH of 2.0, whereafter the yield of ginsenoside Rg3 and CK was 2.16 mg/g and 2.80 mg/g, respectively. For the ginsenoside nanoemulsions with 0.5% WPI as emulsifier, as Tween 80 increased from 0.1% to 0.5%, the mean droplet diameter gradually decreased from ∼740 nm to ∼310 nm. Accordingly, the encapsulation efficiency of Rg3 and CK increased by 8.36% and 9.03%, respectively, and the centrifugal stability constant increased by 49.82%. These results showed that nanoemulsion prepared with 0.5% WPI and 0.5% Tween 80 exhibited higher encapsulation efficiency and physicochemical stability. In addition, ginsenoside nanoemulsion prepared with 0.5% WPI and 0.5% Tween 80 showed great industrial applicability during freeze-thaw cycles, heat treatments and storage. Generally, the incorporation of Tween 80 as a co-emulsifier improved the encapsulation efficiency and physicochemical stability of WPI-stabilized ginsenoside nanoemulsions, which may improve its bioaccessibility, by stabilizing ginsenoside emulsions during further processing, such as during thermal pasteurization and microencapsulation .
研究领域:
食品科学
·
药学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Xiaoxiao Sun,Chuqiao Pan,Zhuoyang Ying,Dongyan Yu,Xitai Duan,Fangfang Huang,Junhong Ling,Xiao-kun Ouyang
We aimed to develop a stable nanosystem with k -carrageenan (Kc), an anionic polysaccharide , combined with tween 80 to stabilize zein nanoparticles. The experimental results showed that the particle size, zeta-potential, and stability of the system depend on the Kc amount when the zein amount was unchanged. We found that the nanosystem with 5.0 and 10.0 mg Kc had a smaller particle size (250.1 ± 0.9 and 287.6 ± 1.4 nm, respectively), low polydispersity index (PDI, ≤ 0.20), and high stability when the external conditions changed. No obvious flocculation or deposition was observed at pH of 3–9, salt ion concentrations of 0–500 mmol/L, temperature of 30 °C and 50 °C–90 °C, and storage at 4 °C for 30 days. When the Kc-ZNP system was loaded with curcumin (Cur), there was a good encapsulation effect (5.0 mg Kc with 1.00 mg Cur (EE (%) = 34.69% ± 2.02%, size = 380.23 ± 5.80 nm, PDI = 0.252 ± 0.018) and 10.0 mg Kc with 2.00 mg Cur (EE (%) = 20.13% ± 1.05%, size = 431.27 ± 0.78 nm, PDI = 0.269 ± 0.020), ensuring that the particle size was not too large. Therefore, Kc–ZNPs could be a potential delivery system for fat-soluble drugs.
研究领域:
食品科学
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药物递送系统
·
生物材料
✓ 已验证
DOI
高影响因子
INTERNATIONAL MICROBIOLOGY
·
2024
·
IF 2.3
Zhang Mingle,He Zhimin,Xu Xiaoyi,Ji Fan,Wang Bin
This study investigates the advantages of combined microbial degradation of polycyclic aromatic hydrocarbons (PAHs) in reducing the inhibitory effects of high-concentration eluents commonly used in soil washing. A microbial synergistic strategy was proposed using Arthrobacter sp. SZ-3 and Pseudomonas putida B6-2 as the key bacteria in the presence of Tween 80. The results show that in systems with Tween 80, the SZ-3 strain exhibits a strong capacity to degrade three types of PAH compounds, while the B6-2 strain follows multiple degradation pathways. Mixed bacteria achieved degradation rates 60.70% higher than single bacteria at varying concentrations of Tween 80. Additionally, the average growth rates of mixed bacteria increased by 1.17–1.37 times, aligning with the changes in the functional group. Protein activity detection within each degradation system corresponded with growth quantity and the cyclic variation characteristics of ETS enzyme activity. Notably, the ETS activity of mixed bacteria was 150% higher than that of single bacteria. At a Tween 80 concentration of 500 mg/L, the degradation rates of PAHs (Phe, Flu, Pyr) by mixed bacteria were significantly higher than those by single bacteria. The catechol 1,2-dioxygenase activity of mixed bacteria was 2.30 times higher than that of single bacteria. While Tween 80 did not alter the PAH degradation pathways, it significantly influenced the accumulation amount and duration of the characteristic intermediate product. This provides a reference for the remediation of recalcitrant pollutants under conditions involving high-concentration surfactants.
研究领域:
环境微生物学
·
污染修复
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMASS & BIOENERGY
·
2020
·
IF 3.551
Wei Yang,Huan Fan,Min Zhou,Zheng Zhou,Lishi Yan,Xin Ju,Liangzhi Li
Stable cellulases are critical for in situ hydrolysis of ionic liquid-pretreated lignocellulose , and surfactants were reported to improve the stability of cellulase in practical circumstances. In this study, synergistic effect of Tween-80 and [Emim]OAc was first identified to enhance the hydrolysis of lignocellulose by Paenibacillus sp. LLZ1 cellulase, proving the effectiveness of surfactants in in situ hydrolysis. Protein fluorescence and near-UV circular dichroism spectroscopy analyses illustrated that ionic liquids might move local residues and alter global tertiary structure of cellulase, leading to higher instability. The addition of Tween-80 might turn the microenvironment of cellulase residues more hydrophilic and reduce the contact of protein with deactivation factors. The synergistic system was further applied in the in situ hydrolysis of microcrystalline cellulose and bagasse cellulose by Paenibacillus cellulase. The enzymatic hydrolysis rate of microcrystalline cellulose was enhanced by 2.5-fold and the final conversion was improved by 45%. Scanning electron microscopy demonstrated the substrate solubilizing effect during reaction by monitoring the morphological changes of lignocellulosic substrates . This study highlighted the potential of applying synergistic reaction system in in situ enzymatic hydrolysis of lignocellulose, and revealed the inherent mechanism initially.
研究领域:
生物化学
·
可再生资源与环境科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Pharmaceutical Innovation
·
2019
·Vol. 15
·期 1
·155-162
·
IF 1.452
Huang Zhengwei,Wu Mingjun,Ma Cheng,Bai Xuequn,Zhang Xuejuan,Zhao Ziyu,Huang Ying,Pan Xin,Wu Chuanbin
Objective Solid lipid nanoparticles (SLN) mainly consist of lipids and surfactants. However, the toxicity of surfactant cannot be ignored, as the surfactant may cause unexpected side effect. This study established model SLN with different surfactants, Tween 80 (T80) and poloxamer 188 (P188). It was aimed to establish ultraviolet-visible (UV-Vis) spectroscopy methods for quantifying the contents of T80 and P188 in the model SLN. Methods The model SLN were produced by emulsification-precipitation method, and their particle size distribution was analyzed. The calibration curves for the contents of T80 and P188 were established. Surfactants in the model SLN were extracted by ultrasonication, and UV-Vis spectroscopy methods were utilized for quantification of surfactants in SLN. Results The deviation of precision and accuracy in P188 and T80 assay were acceptable, which indicated the method could be employed in sample quantification. Ten minutes was considered as the optimal ultrasonication time. And the contents of P188 and T80 were successfully measured by the established UV-Vis methods. Conclusion The UV-Vis spectroscopy methods were developed for the quantification of T80 and P188 in the model SLN. It is expected that the methods could be extended to more SLN systems and applied for the quantification of other surfactants.
研究领域:
药剂学
·
分析化学
✓ 已验证
DOI
高影响因子
BIOTECHNOLOGY AND BIOENGINEERING
·
2016
·Vol. 114
·期 3
·543-551
·
IF 4.243
Renliang Huang,Hong Guo,Rongxin Su,Wei Qi,Zhimin He
Recycling cellulases by substrate adsorption is a promising strategy for reducing the enzyme cost of cellulosic ethanol production. However, β-glucosidase has no carbohydrate-binding module (CBM). Thus, additional enzymes are required in each cycle to achieve a high ethanol yield. In this study, we report a new method of recycling cellulases without β-glucosidase supplementation using lignocellulosic substrate, an engineered strain expressing β-glucosidase and Tween 80. The cellulases and Tween 80 were added to an aqueous suspension of diluted sulfuric acid/ammonia-treated corncobs in a simultaneous saccharification and fermentation (SSF) process for ethanol production. Subsequently, the addition of fresh pretreated corncobs to the fermentation liquor and remaining solid residue provided substrates with absorbed cellulases for the next SSF cycle. This method provided excellent ethanol production in three successive SSF cycles without requiring the addition of new cellulases. For a 10% (w/v) solid loading, a cellulase dosage of 30 filter paper units (FPU)/g cellulose, 0.5% Tween 80, and 2 g/L of the engineered strain, approximately 90% of the initial ethanol concentration from the first SSF process was obtained in the next two SSF processes, with a total ethanol production of 306.27 g/kg corncobs and an enzyme productivity of 0.044 g/FPU. Tween 80 played an important role in enhancing cellulase recovery. This new enzyme recycling method is more efficient and practical than other reported methods. Biotechnol. Bioeng. 2017;114: 543–551. © 2016 Wiley Periodicals, Inc.
研究领域:
生物技术
·
酶工程
·
发酵工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2010
·
IF 1.988
Guolin Song,Sude Ma,Guoyi Tang,Xiaowei Wang
In this paper, with the aid of ultrasonic irradiation, hydrophobic magnesium hydroxide (MH) nanoparticles were synthesized via one-step precipitation reaction, in which the reactant solutions were simultaneously added into an organic phase instead of an aqueous phase. Tween 80 was added as a stabilizer , dispersant and surface modifier. During this synthesis process, ultrasonic irradiation could restrict the crystal growth of MH on each lattice plane and improve hydrophobicity of MH nanoparticles. Addition of Tween 80 was favourable to obtain smaller sized MH nanoparticles with even crystal growth and better dispersibility, and to further enhance the hydrophobic property of MH nanoparticles based on its bridging function between surface of MH nanoparticles and aliphatic compounds in organic phase. In addition, the morphology and the properties of obtained MH nanoparticles were characterized by XRD , FESEM , FT-IR, TGA , respectively. The improved hydrophobicity and dispersibility of surface modified MH nanoparticles were also verified by the sedimentation tests.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
·
2026
·
IF 3.9
Liu Yating,Zhong Jie,Gan Na,Chen Lijuan,Liu Peiran,Xu Junjie,Geng Fang,Li Tingting,Wu Di
To tackle the high volatility, hydrophobicity and instability that limited cinnamaldehyde (CMA) in practical applications, CMA nanoemulsion (CNE) synergistically stabilized by cinnamic acid (CA) and Tween-80 were successfully prepared and characterized by ultrasonic emulsification treatment. The results showed that the CNE particle size was uniform (105.54 ± 2.14 nm). The CMA in CNE showed better slow-release properties under sealed conditions (22.68 ± 2.81% on day 10). The heat stability, freeze–thaw stability and storage stability of CNE showed good stability during 10 d storage period. Sensory and physicochemical property analyses indicated that the CNE was released headspace under non-contact conditions, reducing the impact on the flavor of chilled beef. It also effectively mitigated the increase in pH, juice loss and the increase in total volatile base nitrogen (TVB-N), thiobarbituric acid-reactive substance (TBARS), and total number of microbial colonies (TNC) of chilled beef during refrigeration. Graphical abstract
✓ 已验证
产品关联
DOI
高影响因子
Journal of Oleo Science
·
2024
·
IF 1.6
Aicha Mekkaoui,You Li,Zhaoming Ran,Ying Cai,Li Zhao,Baocai Xu,Ce Wang
In recent years, there has been a growing interest in regulating lipid digestion through the construction of various interfacial structures. In the present work, a series of complex interfacial structures were designed by combining Tween 80 in the aqueous phase and lecithin in the oil phase at different concentration ratios. The emulsification properties, the roles in regulating lipid digestion, and the interfacial dilatational rheological properties of the composite emulsifying systems were characterized. The results showed that the combination of Tween 80 and lecithin at different ratios could effectively modulate the rate of lipid digestion. The polyoxyethylene chains of Tween 80 formed a network, that provided a spatial obstacle for the adsorption of bile salts and lipases. Thus, Tween 80 significantly delayed the lipid digestion. The introduction of lecithin gradually replaced Tween 80 molecules at the interface, thus providing space for the adsorption of bile salts and lipases. In addition, as the ratio of lecithin concentration to Tween 80 increased, lecithin gradually became the dominant factor in the interfacial properties. As a result, the rate of lipid digestion was accelerated. Therefore, by compounding different ratios of lecithin and Tween 80, a series of emulsions with different lipid digestion rates were obtained. This research provides a basis for rationally designing food emulsions according to specific needs.
研究领域:
食品科学
·
化学工程
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
Food and Bioprocess Technology
·
2014
·Vol. 7
·期 11
·3121-3135
·
IF 3.126
Tian Ye,Fu Nan,Wu Winston Duo,Zhu Danhua,Huang Jinjian,Yun Shuo,Chen Xiao Dong
Dairy industries often spray lecithin, a food-grade surfactant, to spray-dried whole milk powders while fluidisation to produce instant powders. Though adding surfactant to milk feed was often reported to improve the wettability of dried powders, this approach was not favourably used. The present study investigated the effects of surfactant addition into high solids milk feed before drying on the wettability of whole milk particles. Adding either 0.1 wt.% Tween 80 or 0.1 wt.% lecithin to un-concentrated whole milk led to a significant wettability improvement of spray-dried powders. At higher feed solids levels of 23 and 33 wt.%, the wetting process of pure milk powders was comparatively rapid, but the surfactant-added powders showed similar wettability to the pure milk powders. The development of powder wettability as drying progressed was investigated using single droplet-drying technique for 32 and 43 wt.% whole milk in the presence and absence with surfactants. The technique captured an advanced shell formation during drying of higher solids milk. The wettability of surface shells formed by surfactant-added milk was similar to those formed by pure milk throughout drying, from initial shell formation to final drying stage. By contrast, coating surfactants on the outer layer of particles being dried could substantially improve wettability. The rapid shell formation and the slow material diffusion owing to the high medium viscosity were considered the main factors limiting the migration of surfactant molecules towards droplet surface during drying.
研究领域:
食品科学
·
乳品加工
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）