FOOD CONTROL
·
2024
·
IF 5.6
Kuiyu Wang,Tianying Sun,Liangke Gong,Lanxiang Zeng,Jianwei Liang,Yayue Huang,Yuanye Wang,Tao Yang,Hui Zeng
At present, most reported studies on paper-based sensing devices for food-borne pathogens usually rely on a single signal as the basis for judging the results, accompanied with inaccurate detection results. In this study, we used the lanthanide MOFs being covalently modified with aptamers (Apt-LnMOFs) as the sensing and recognition element of Vibrio parahaemolyticus (VP), and the paper-based needle shape device (paper-needle) as the visual signal output carrier. Finally, an accurate detection strategy for VP was successfully constructed. In terms of signal output, the two signal styles of Apt-LnMOFs diffusion distance on the paper-needle and R value (measured by the RGB analysis software of smartphone) are coupled to jointly improve the accuracy of detection results. The method is simple and fast, and the detection accuracy in real samples is 91%. This strategy is very suitable for on-site rapid detection of pathogens, and does not require large professional instruments.
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Frontiers in Microbiology
·
2021
·
IF 5.64
Wang Wenbin,Liu Jianxin,Guo Shanshan,Liu Lei,Yuan Qianyun,Guo Lei,Pan Saikun
The discovery of outer membrane proteins (OMPs) with desirable specificity and surface availability is a fundamental challenge to develop accurate immunodiagnostic assay and multivalent vaccine of pathogenic Vibrio species in food and aquaculture. Herein 101 OMPs were systemically screened from 4,831 non-redundant proteins of Vibrio parahaemolyticus by bioinformatical predication of signaling peptides, transmembrane (TM) α-helix, and subcellular location. The sequence homology analysis with 32 species of Vibrio spp. and all the non-Vibrio strains revealed that 15 OMPs were conserved in at least 23 Vibrio species, including BamA (VP2310), GspD (VP0133), Tolc (VP0425), OmpK (VP2362), OmpW (VPA0096), LptD (VP0339), Pal (VP1061), flagellar L-ring protein (VP0782), flagellar protein MotY (VP2111), hypothetical protein (VP1713), fimbrial assembly protein (VP2746), VacJ lipoprotein (VP2214), agglutination protein (VP1634), and lipoprotein (VP1267), Chitobiase (VP0755); high adhesion probability of flgH, LptD, OmpK, and OmpW indicated they were potential multivalent Vibrio vaccine candidates. V. parahaemolyticus OMPs were found to share high homology with at least one or two Vibrio species, 19 OMPs including OmpA like protein (VPA073), CsuD (VPA1504), and MtrC (VP1220) were found relatively specific to V. parahaemolyticus. The surface proteomic study by enzymatical shaving the cells showed the capsular polysaccharides most likely limited the protease action, while the glycosidases improved the availability of OMPs to trypsin. The OmpA (VPA1186, VPA0248, VP0764), Omp (VPA0166), OmpU (VP2467), BamA (VP2310), TolC (VP0425), GspD (VP0133), OmpK (VP2362), lpp (VPA1469), Pal (VP1061), agglutination protein (VP1634), and putative iron (III) compound receptor (VPA1435) have better availability on the cell surface.
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ANALYTICAL BIOCHEMISTRY
·
2024
·
IF 2.6
Lin Tian,Yang Li,Huiqing Wang,Xinyi Li,Qian Gao,Yaru Liu,Yao Liu,Qing Wang,Cuiping Ma,Chao Shi
Vibrio parahaemolyticus ( V. parahaemolyticus ) is a major foodborne pathogen , which can cause serious foodborne illnesses like diarrhoea. Rapid on-site detection of foodborne pathogens is an ideal way to respond to foodborne illnesses. Herein, we provide an electrochemical sensor for rapid on-site detection. This sensor utilized a pH-sensitive metal-oxide material for the concurrent isothermal amplification and label-free detection of nucleic acids . Based on a pH-sensitive hydrated iridium oxide oxyhydroxide film (HIROF), the electrode transforms the hydrogen ion compound generated during nucleic acid amplification into potential, so as to achieve a real-time detection. The results can be transmitted to a smartphone via Bluetooth. Moreover, HIROF was applied in nucleic acid device detection, with a super-Nernst sensitivity of 77.6 mV/pH in the pH range of 6.0–8.5, and the sensitivity showed the best results so far. Detection of V. parahaemolyticus by this novel method showed a detection limit of 1.0 × 10 3 CFU/mL, while the time consumption was only 30 min, outperforming real-time fluorescence loop-mediated isothermal amplification (LAMP). Therefore, the characteristics of compact, portable, and fast make the sensor more widely used in on-site detection.
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FOOD CONTROL
·
2016
·
IF 3.388
Can Xu,Jinglei Li,Liuqing Yang,Fang Shi,Liu Yang,Ming Ye
This study aimed to investigate the antibacterial activity of Lachnum YM30 intracellular melanin (LIM). The dilution method was used to measure the MIC and MBC of Gram-negative bacteria Escherichia coli, Salmonella typhi , Vibrio parahaemolyticus and Gram-positive bacteria Listeria monocytogenes , Bacillus megaterium , Staphylococcus aureus . MIC values for each bacterial strain were 0.4, 2.4, 0.2, 0.6, 0.8, 0.3 mg/mL, and the MBC values were 0.4, 3.2, 0.3, 0.8, 0.8, 0.4 mg/mL, respectively. Bacteriostasis of LIM against S. aureus (G + ) and V. parahaemolyticus (G − ) was further revealed by SEM observation, testing integrity of cell membrane, NPN uptake and membrane potential. MTT experiment was used to estimate the cell cytotoxicity of LIM. The results showed that LIM significantly damaged the integrity of cell membrane, increased the leakage of cell contents, raised the quantity of NPN uptake and reduced the membrane potential. LIM had no obvious cell cytotoxicity at low concentrations. These findings indicated that LIM had antibacterial activity and could be applied as a potential natural bacteriostatic agent in the food or pharmaceutical industry.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Wenhai Wang,Shu Xiao,Mengli Zeng,Hongzhen Xie,Ning Gan
Rapid and accurate detection of Vibrio parahaemolyticus (VP) in a variety of testing environments is critical for preventing the spread of foodborne illnesses. Therefore, a dual-mode colorimetric-electrochemical biosensor was prepared for the rapid and sensitive detection of VP . Phenylboronic acid-decorated CuO 2 nanodot-mediated organic metal-organic framework (CP@MOF) nanozymes were constructed to produce colorimetric and electrochemical signals. Furthermore, an electrode surface was fabricated using tetrahedral DNA nanostructure-functionalized poly(amidoamine) dendrimer-Au nanoparticles to specifically bind VP . Under acidic environments, the CP@MOF nanozymes disintegrated, enabling the simultaneous release of Cu 2+ and H 2 O 2 . This in turn causes a Fenton-type reaction, generating a hydroxyl radical , which then oxidizes 3,3′,5,5′-tetramethylbenzidine, thereby enabling visual detection of VP . Moreover, the produced copper ions can be used for the electrochemical detection of VP . This dual-mode biosensor achieved an accuracy of 88.7 %, which was higher than that of single-mode assays (81.1 %). Hence, this dual-mode assay may facilitate the effective on-site detection of pathogens in shrimp samples.
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Scientific Reports
·
2020
·Vol. 10
·期 1
·1-8
·
IF 3.998
Tan Xiao-Hui,Li Yu-Bin,Liao Yan,Liu Hua-Zhong
In this work, an enzyme-free fluorescence resonance energy transfer (FRET) strategy was established for rapid and specific detection of the DNA sequence from Vibrio parahaemolyticus (VP) using hybridization chain reaction (HCR) amplification and triplex DNA. The triplex forming oligonucleotide (TFO) was labelled with carboxyfluorescein (FAM) as fluorescence donor, and hairpin sequence H1 was labelled by tetramethylrhodamine (TAMRA) as fluorescence receptor. In the present target VP DNA, the hairpin structure of molecular beacon (MB) was opened, the free end was released and hybridized with H1-TAMRA, and the HCR reaction was triggered by the alternate supplementation of H1-TAMRA and H2 to produce the notch double helix analogue. After the addition of TFO-FAM, a triplex structure was formed between HCR products (H1-TAMRA/H2) and TFO-FAM. A close contact between the donor and the receptor resulted in FRET. Under the optimal conditions, the fluorescence quenching value was inversely proportional to the concentration of target VP DNA in the range of 0.1–50 nmol L −1 , and the detection limit was 35 pmol L −1 .
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FOOD CHEMISTRY
·
2019
·
IF 5.399
Xuetong Li,Yuan Su,Huashuo Chu,Shuxia Lyu,Jingjing Tian,Wentao Xu
Vibrio parahaemolyticus is a major hidden danger of food safety. To develop a rapid, sensitive and on-site detecting method of Vibrio parahaemolyticus ( V. parahaemolyticus ), a strand replacement primer thermostat phosphate (SRPP) visual sensor was proposed, based on Bst DNA polymerase and pyrophosphatase. The novel strand replacement primer (SRP) facilitates chain substitution and to open a self-folding hairpin by adding region at its 3′ end. Under the action of the SRP, a pair of external primers and two inner primers, target DNA is specifically amplified at 63 °C relies mainly on the hairpin. Many pyrophosphates (PPi) are simultaneously generated as by-products, which can be converted into phosphates (Pi) by pyrophosphatase for phosphomolybdate blue visual detection within 5 min. The proposed biosensor can detect 1.29 × 10 3 copies of V. parahaemolyticus within 35 min.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Qingqing Zhang,Tingting Zhai,Yuxin Guo,Yifan Weng,Nan Zhou,Han Lin,Hao Tan,Kangning Lu,Yiyi Zhou
Highly selective and sensitive detection of foodborne pathogens is crucial for ensuring food safety. In this work, a photoelectrochemical (PEC) aptasensing platform was developed for the selective and sensitive detection of Vibrio parahaemolyticus (VP), adopting Faraday cage-type construction mode. When VP was present in the sample, the aptamer for VP (Apt) assembled on the Apt/Bi 2 S 3 /ITO electrode captured VP, which bound GO@Cu 2 O-Apt to the electrode surface. The graphene oxide (GO) in GO@Cu 2 O-Apt has a large surface area and good conductivity, on the surface of which a large amount of Cu 2 O that can switch the polarity of the Bi 2 S 3 photocurrent was loaded, resulting in a highly selective and sensitive detection of VP with a detection limit of 1.0 CFU/mL. By adjusting the Apt, the platform can be used for the detection of other foodborne pathogens, offering broad application potential in foodborne pathogen detection.
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Foods
·
2025
·Vol. 14
·期 14
·2481
·
IF 5.1
Wei Yan,Liping Chen,Lei Ji,Rui Yuan,Fenfen Dong,Peng Zhang
Vibrio parahaemolyticusis the leading bacterial cause of gastroenteritis associated with aquatic food consumption globally. This study aimed to determine the prevalence ofV. parahaemolyticusin aquatic foods from Huzhou and to identify the serotypes, antimicrobial resistance, virulence factors, and genetic relatedness of the strains. A total of 306 isolates were detected from 1314 aquatic food samples from 2022 to 2024. The results indicated that the most prevalent serotypes were O1:KUT (17.0%), O2:K28 (13.7%), and O2:KUT (13.1%). Multilocus sequence typing analysis divided the 306 isolates into 175 sequence types (STs), and the predominant sequence type was ST864 (3.3%). Antimicrobial susceptibility tests showed that 2.6% of isolates were multidrug resistant. High resistance was observed to ampicillin (64.7%) and streptomycin (44.4%). A total of seven antimicrobial categories of resistance genes were identified, and the resistance geneblaCARBwas detected in all isolates. The virulence genestdhandtrhwere found in 16 (5.2%) and 12 (3.9%) isolates, respectively. In addition, we observed that all the 306V. parahaemolyticusisolates encode type III secretion systems 1. The phylogenomic analysis based on the whole-genome sequence revealed that the 306 isolates were divided into four clusters. Our findings broaden perspectives onV. parahaemolyticusgenetic diversity and enhance our ability to assess the potential risks of its spread.
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ELECTROCHIMICA ACTA
·
2024
·
IF 5.5
Yuhao Zhang,Luting Yan,Lei Tian,Qi Guo,Maosheng Sang,Shouyi Dou,Falan Li,Qingqing Yang,Xia Sun,Yemin Guo,Yanyan Zhang
Vibrio parahaemolyticus ( VP ) is one of the most common pathogens in aquatic products, and its study using electrochemiluminescence is still relatively rare. In this study, an electrochemiluminescent (ECL) biosensor was developed to detect VP in aquatic products using AuNPs modified PEI/MWCNTs (AuNPs@PEI/MWCNTs) as a substrate material and ordered mesoporous carbon (OMC) adsorbed Ru(bpy) 3 2+ (OMC@Ru(bpy) 3 2+ ) as a luminescent material. To increase the conductivity of the biosensor and offer a favorable loading environment for the immobilization of the luminescent probe, AuNPs@PEI/MWCNTs are employed as the substrate. Meanwhile, the OMC@Ru(bpy) 3 2+ functionalized DNA probe acts as a luminescent and capture probe to generate ECL signals and specifically recognize different concentrations of VP . The biosensor has good selectivity and stability and has been successfully applied to the detection of VP in scallops. This method not only expands the potential uses of bioprobe-based ECL biosensors, but also offers a new and effective approach used to detect food-borne pathogens in aquatic products.
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BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Hui Wu,Yanju Chen,Qunqing Yang,Cheng Peng,Xiaofu Wang,Mengyao Zhang,Siwenjie Qian,Junfeng Xu,Jian Wu
Vibrio parahaemolyticus ( V. parahaemolyticus ) is regarded as a major cause of seafood-associated illnesses, which has aroused widespread public concern. Here, a rapid and convenient detection method for V. parahaemolyticus detection was established by a reversible valve-assisted chip coupling with CRISPR/Cas12a. With optimized lysis buffer, loop mediated isothermal amplification (LAMP) reagents and CRISPR reagents, the whole detection process from sampling to results could be finished within 50 min. The structure of chip was simple and the cost was low. By relying on three reversible rotary valves and the rotation direction-dependent Coriolis pseudo force, the flow order of liquid and the direction of liquid flow could be precisely controlled. The LAMP amplicons were specifically and sensitively identified by CRISPR/Cas12a. Positive amplification would produce green fluorescent signal while negative amplification generated no fluorescent signal, which could be clearly distinguished by the naked eye. With 600 μL of samples processed, the limit of detection (LOD) for both pure cultured V. parahaemolyticus or spiked shrimp samples could achieve 30 copies/reaction. These illustrated the established method displayed great feasibility for real samples detection. In the future, the chip could also combine with other amplification reactions, like PCR or recombinase polymerase amplification reaction (RPA), to conduct detection by changing the corresponding lyophilized amplification reagents. Overall, the proposed detection platform displays great potential for food safety analysis and clinical diagnostics, especially in resource-limited areas.
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CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Fengyan Liang,Chengbo Sun,Sidong Li,Tingting Hou,Chengpeng Li
To explore the disease resistance mechanism of chitosan conjugates , chitosan-gentamicin conjugate (CS-GT) was synthesized and systematically characterized, the immune mechanism of CS-GT on Litopenaeus vannamei infected with Vibrio parahaemolyticus was further explored. The results showed that imine groups in CS-GT were effectively reduced. Dietary supplementation of CS-GT can significantly increase the survival rate, total hemocyte counts, the antioxidant and immune related enzyme activity levels of shrimps ( P < 0.05), which are all dose-dependent under the experimental conditions. In addition, CS-GT can protect the hepatopancreas from invading bacteria and alleviate inflammation. Particularly, CS-GT promotes the expressions of legumain (LGMN), lysosomal acid lipase (LIPA) and Niemann-Pick type C2 (NPC2) up-regulated. It is speculated that CS-GT may stimulate the lysosome to phagocytose pathogens more effectively. In conclusions, shrimps fed with CS-GT can produce immune response via lysosome and greatly improve the disease resistance to Vibrio parahaemolyticus .
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