BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Xiaohai Yan,Gengan Du,Hong Chen,Qiannan Zhao,Qi Guo,Jianlong Wang,Zhouli Wang,Wei Song,Qinglin Sheng,Yane Luo,Yahong Yuan,Tianli Yue
Patulin (PAT) is an unsaturated lactone mycotoxin primarily produced by Penicillium expansum and Aspergillus clavatus . Given the potential health risks and economic losses associated with PAT, the rapid detection of PAT using fluorescent aptasensors is of significant importance in evaluating food safety. However, it easily increases the cost and complexity caused by signal labeling. We combined TCPP/BDC-NH 2 mixed ligands functionalized Zr metal–organic frameworks (Zr-MOF mix ) and terminated three-stranded DNA gates (ttsDNA gates) to fabricate a label-free fluorescent aptasensor for PAT detection. The Zr-MOF mix system was synthesized via a one-pot strategy and could be used to address the problem of pore size limitation and increase the loading amounts of dyes. TtsDNA gate was integrated into the Zr-MOF mix system to control the release of dyes, exhibiting a high signal-to-background ratio. The single-stranded aptamer region in ttsDNA gate situated away from the surface of the Zr-MOF mix , resulting in a natural release of dyes in the absence of PAT. While binding to PAT resulted in target-induced conformational changes that helped form the hairpin structure of the aptamer. This structure hindered the release of dyes from the pores of Zr-MOF mix , thus reducing the fluorescence signals intensity. The stimuli-responsive DNA-gated material provides a platform for PAT analysis under conditions of a low limit of detection (0.871 pg/mL). Furthermore, the excellent specificity and anti-interference of the fluorescent aptasensor make the system suitable for the analysis of apple juice samples. This label-free strategy is cheaper and simper compared with labeled detection, especially for the development of multi-target-detection.
研究领域:
食品安全
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生物传感
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分子生物学
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产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2024
·
IF 6.6
Yan Yang,Yiling Wang,Xinran Jiang,Jiahao Mi,Dizhang Ge,Yuna Tong,Yuxuan Zhu
Introduction Alzheimer’s disease (AD), a neurodegenerative condition, stands as the most prevalent form of dementia. Its complex pathological mechanisms and the formidable blood-brain barrier (BBB) pose significant challenges to current treatment approaches. Oxidative stress is recognized as a central factor in AD, underscoring the importance of antioxidative strategies in its treatment. In this study, we developed a novel brain-targeted nanoparticle, Ce/Zr-MOF@Cur-Lf, for AD therapy.Methods Layer-by-layer self-assembly technology was used to prepare Ce/Zr-MOF@Cur-Lf. In addition, the effect on the intracellular reactive oxygen species level, the uptake effect by PC12 and bEnd.3 cells and the in vitro BBB permeation effect were investigated. Finally, the mouse AD model was established by intrahippocampal injection of Aβ1-42, and the in vivo biodistribution, AD therapeutic effect and biosafety of the nanoparticles were researched at the animal level.Results As anticipated, Ce/Zr-MOF@Cur-Lf demonstrated efficient BBB penetration and uptake by PC12 cells, leading to attenuation of H2O2-induced oxidative damage. Moreover, intravenous administration of Ce/Zr-MOF@Cur-Lf resulted in rapid brain access and improvement of various pathological features of AD, including neuronal damage, amyloid-β deposition, dysregulated central cholinergic system, oxidative stress, and neuroinflammation.Conclusion Overall, Ce/Zr-MOF@Cur-Lf represents a promising approach for precise brain targeting and multi-target mechanisms in AD therapy, potentially serving as a viable option for future clinical treatment.
研究领域:
神经科学
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药学
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纳米技术
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痴呆症研究
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Zarfashan Shahid,Bairong Fu,Saima Rehman,Zia Ahmad,Jiping Chen,Xianbo Lu
Bisphenol compounds, including BPA and BPS, are significant pollutants found in the environment, food, and daily necessities. They pose various adverse effects, including estrogenic, immunotoxic, metabolic, and reproductive toxicity, which severely threaten human health and ecological safety. Achieving simultaneous rapid detection of BPA and BPS is still very challenging due to their similar structures and co-existence in actual samples at ultratrace level. This study addresses the problem of developing an effective method for the rapid detection of BPA and BPS simultaneously. A new electrochemical nanosensor based on ionic liquid (IL) coupled Zr-MOF@GO was developed for ultrasensitive real-time detection of BPA and BPS. The Zr-MOF@GO/IL nanocomposite features a custom-built structure with an ordered framework, abundant pores, and highly coupled interfaces. The experimental findings demonstrate that the introduction of IL enhances the conductivity and dispersibility of the nanocomposite, improves the absorption capability for target bisphenols, and significantly improves the detection limit and linear range of the Zr-MOF@GO based electrochemical sensor. The sensor demonstrated wide linear ranges with detection limits of 5.5 nmol/L for BPA and 7.1 nmol/L for BPS (S/N = 3), respectively. Comparative studies revealed that the detection limits of the Zr-MOF@GO/IL-based sensor improved by an order of magnitude compared to the Zr-MOF@GO-based sensor. Additionally, the sensor exhibited superior analytical performance regarding reproducibility, stability, and selectivity. This study investigated the capability and synergy of IL coupled high-valent stable transition metal Zr IV -based MOFs in electrochemical sensing for the first time. This development sets a new benchmark in non-enzymatic electrochemical sensing, establishing the Zr-MOF@GO/IL-based sensor as a promising platform for the individual or simultaneous rapid detection of bisphenols in actual samples.
研究领域:
环境科学
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分析化学
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材料科学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2020
·
IF 3.573
Mingbo Shi,Daiwu Lin,Renliang Huang,Wei Qi,Rongxin Su,Zhimin He
The treatment of multicontaminant wastewater has attracted extensive attention in recent years. Herein, we synthesized a porous and hydrophobic composite (Zr-MOF-SH/MF) via the in situ growth of mercapto-functionalized Zr-metal–organic frameworks (MOFs) (Zr-MOF-SH) on the surface of a melamine formaldehyde (MF) sponge. The successful growth of hydrophobic Zr-MOF-SH, as confirmed by SEM, XRD, and XPS analysis, made the MF sponge become hydrophobic with a water contact angle of 125°. The adsorption capacity of the Zr-MOF-SH/MF composite for dichloromethane can reach up to 113 g/g. Moreover, the composite can efficiently and selectively remove Hg2+ ions from water. Especially, PCN-224-MAA/MF had an adsorption capacity of 412.5 mg/g for Hg2+ ions. The removal efficiency of Hg2+ ions increased as the oil content in the oil–water mixture increased. In addition, PCN-224-MAA/MF can be recovered and reused five times with only a slight decrease in adsorption capacity. This study provides a promising strategy for the synthesis of a dual-functional composite that exhibits excellent performance in removing oil and heavy metal ions simultaneously from water.
研究领域:
环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Wenrong Cai,Yanjing Shi,Datong Wu,Junyao Li,Laidi Xu,Yong Kong
This work proposes a novel dual-signal electrochemiluminescence ( ECL) immunosensor based on energy resonance transfer (RET) for the detection of β2-Microglobulin (β2-MG). Introducing carbon vacancy into g-C 3 N 4 (V C -g-C 3 N 4 ) can lead to the narrowing of the band gap and the reduction of LUMO, thereby significantly enhancing the ECL efficiency of the material. Meanwhile, Zr-MOF can effectively load Ru(bpy) 3 2+ (Ru-Zr-MOF), enabling its stable existence on the electrode. The ECL emission spectrum of V C -g-C 3 N 4 overlaps well with the fluorescence excitation spectrum of Ru-Zr-MOF, indicating an effective ECL-RET effect between them. As the concentration of β2-MG increases, the intensity of anodic ECL signal gradually increases, while the intensity of cathodic ECL signal gradually decreases. The difference between the cathodic and anodic ECL signals has a good linear relationship within the concentration range from 0.2 pg/mL to 200 ng/mL, with a detection limit of 27.73 fg/mL. The constructed immunosensor can be used for practical analysis in human serum. The proposed sensor has great potential in clinical detection of β2-MG, providing new insights for biomolecular detection and disease diagnosis.
研究领域:
生物化学
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分析化学
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生物医学工程
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临床诊断
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
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2020
·Vol. 25
·期 20
·4673
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IF 3.267
Zhu Ding,Xiao Min Zhang,Xue Chang,Shuo Wang,Dan-Hong Wang,Ming Hui Zhang,Tian Hao Zhang
Metal-Organic Framework (MOF) materials are often modified or functionalized, and then the crystal size and morphology of MOF materials are changed. In the process of preparing UiO-66 confined phosphomolybdic acid (PMA) composites (PU), the TiF4-modified PU (PMA + UiO-66) composite catalyst (TiF4-PU) was successfully synthesized by adding titanium tetrafluoride, and the catalytic desulfurization activity was excellent. Similarly, the reaction mechanism was investigated by means of infrared spectroscopy, Raman spectroscopy, XPS, and UV/Vis spectroscopy. The results show that the addition of TiF4not only changes the appearance and color of the catalyst, but also changes the valence distribution of the elements in the catalyst. The number of oxygen vacancies in the MOF increases due to the addition of TiF4, and more electrons are transferred from the Zr-MOF to PMA to form more Mo5+, which improved the performance of oxidative desulfurization in comparison. Thus, a stronger strong metal-support interaction (SMSI) effect is observed for TiF4-modified PU catalysts. In addition, the quenching experiment of free radicals shows that ·OH radical is the main active substance in the oxidative desulfurization reaction over TiF4-PU catalyst.Keywords:MOF materials;Raman spectroscopy;Zr-MOF;SMSI;TiF4-PU;oxidative desulfurization
研究领域:
材料科学
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催化
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化学工程
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光谱学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2022
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IF 10.383
Liangqiong Ren,Feng Hong,Lingwen Zeng,Yiping Chen
To address the urgent demand for sensitive and stable detection applications, significant efforts have been made in the development of dual-signal readout assays for precise target detection and timely health risk control. Here, a new nanomaterial, Pt@PCN-224-HRP-initiator DNA (PP-HRP-iDNA), was exploited to construct a dual-signal readout biosensing platform. Zr-MOF (PCN-224) was loaded with as many Pt nanoparticles (NPs) and as much horseradish peroxidase (HRP) as possible to enhance the brightness of the colorimetric signal recognizable to the naked eye while also acting as a gatekeeper to protect the enzyme activity and ensuring the stability of the assay process. Moreover, the Pt NPs and HRP displayed a synergistic catalytic effect, which promoted the sensitivity of detection. Further, the formation of the Zr–O–P bond eliminated the instability of the interactions between PCN-224 and iDNA in a controllable manner. After the immunoreaction, iDNA stimulated a hybridization chain reaction, resulting in a significant reduction of the fluorescent DNA in the supernatant and a fluorescent signal change. Subsequently, the PP-HRP-iDNA probe implemented UV-light response (450 nm) where 3,3′,5,5′-tetramethylbenzidine was used as a substrate for the colorimetric signal readout. By virtue of the nanomaterial-modulated transduction mechanism and the antigen–antibody interactions, this dual-signal biosensor displays high sensitivity, with a limit of detection of 0.65 pg/mL for aflatoxin B1 and 4 CFU/mL for Salmonella enteritidis, suggesting the detection potential of the biosensing platform for analyzing various targets.
研究领域:
纳米技术
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分析化学
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生物传感器
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材料科学
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食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2022
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IF 16.744
Feng Hong,Liangqiong Ren,Yiping Chen
In this work, we report a fluorescent and colorimetric dual readout bioassay for deoxynivalenol detection to ensure public food safety. A multifunctional fluorescent and colorimetric signal probe was constructed to develop the bioassay for deoxynivalenol detection based on the characteristics of Zr-MOF (PCN-224; i.e., high porosity, a large surface area, and excellent fluorescence quenching performance). Platinum nanoparticles (Pt NPs) were grown in situ outside, while glucose oxidase was stored inside (PPG). Glucose oxidase and Pt NPs are both enriched by Zr-MOF, achieving the first signal amplification, and cascade catalysis by the glucose-TMB system for colorimetric signal readout leads to the second signal amplification. PPG was quenched through the spontaneous formation of Zr O P bonds with fluorescent DNA to achieve a highly sensitive fluorescent readout. The self-supplied H 2 O 2 in the colorimetric detection further improved the self-accuracy of the dual-mode analysis strategy (the accuracy of independent signal readout modes from two different signal transduction mechanisms). This multifunctional PPG probe avoids the time-consuming assembly of different probes and reduces error accumulation from uncontrollable factors in complex experimental procedures. It allows for the sensitive and accurate detection of deoxynivalenol with excellent limits of detection (5.3 pg/mL for the fluorescent assay and 34 pg/mL for the colorimetric method). Overall, this work demonstrates a viable approach for the construction of a dual-signal biosensing platform based on multifunctional MOF probes for biological analysis.
研究领域:
分析化学
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生物化学
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食品安全
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE
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2024
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IF 3.2
Xi Wu,Chenggang Geng,Weiwei Cheng,Zhenjiong Wang,Yan Zhang,Di Wu,Xiaozhi Tang
In this work, an ultrasensitive electrochemical sensor based on Zr-MOF-SH/rGA/NPG was developed for the first time for the rapid determination of mercury ions. First, nanoporous gold (NPG) film was covered on the glassy carbon electrode (GCE) to offer a desirable substrate. Then, Zr-MOF-SH/rGA composites were dropped on the NPG film to form a modified electrode. Mercapto functionalized MOFs (Zr-MOF-SH) showed strong adsorption capability toward mercury ions, and the unique structure of reduced graphene oxide aerogel (rGA) provided various sites for coupling with Zr-MOF-SH as well as improved the electrochemical activity. As a consequence of the synergistic effect of Zr-MOF-SH, rGA, and NPG, the optimized Zr-MOF-SH/rGA/NPG/GCE sensor showed excellent detection performance toward mercury ions with a linear range from 0 to 200 nM and a low limit of detection of 1.4 nM. Meanwhile, the fabricated electrochemical sensor exhibited outstanding stability, reproducibility, and anti-interference ability. To verify the practical applicability, the Zr-MOF-SH/rGA/NPG/GCE was applied for the determination of mercury ions in real rice samples with desirable recovery rates ranging from 98.8% to 108.3%.
研究领域:
电化学
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环境科学
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材料科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2022
·
IF 9.136
Mao Fu,Xuepeng Deng,Shi-Qiang Wang,Fenglin Yang,Li-Chiang Lin,Michael J. Zaworotko,Yingchao Dong
To find sustainable water solutions, the development of high capacity, scalable robust adsorbents and mechanistic insight about their performance offers the potential to effectively address the global challenges of water scarcity and water contamination. We herein rationally design Zr-cluster defective MOF-808 (MOF-808 def ) with exposed carboxyl groups, a robust zirconium metal–organic framework (Zr-MOF), exhibiting high adsorption capacity (q max ∼ 296 mg·g −1 ) coupled with high selectivitity for tetracycline (TC) antibiotics, outperforming other water-stable MOFs, commercial and inorganic nano-adsorbents. MOF-808 def functions well across a wide range of contaminant concentrations (from trace to high-concentration) and even in harsh conditions ( e.g. , high acidity and salinity). Both experimental and simulation results indicate that the mechanism of adsorption involves both physisorption and chemisorption via hydrogen bonding, electrostatic interactions (EIs) and C-O-C covalent bonding via esterification. Computational studies confirm that hydrogen bonding plays a key role in strong guest–host interactions between TCs and MOF-808 def . Further, defects resulting from missing-Zr-clusters in MOF-808 def are confirmed to enhance adsorption performance. Specifically, the defect sites present exposed carboxyl groups from MOF-808 def linker ligands that selectively react with –OH groups (phenol and tertiary alcohol moieties) in TC via esterification. These defects drive highly selective adsorption even at low concentrations of TCs ( e.g. , 500 ppb). Aiming for more than enhanced performance, economic estimation and scalable engineered reactor tests revealed that MOF-808 def and its nano-composites are free of environmental risks and offer promise for sustainable water treatment at pilot scale. The use of defect-engineering rationales is a molecule-level design concept that could be generally useful for the development of the next generation of MOF-based nano-adsorbents for sustainable water treatment applications.
研究领域:
材料科学
·
环境科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yaqi Yu,Dilong Hong,Zeyu Zhu,Chuanfeng Zhao,Yuliang Jiang
Tryptophan(Trp), an essential amino acid for the body, plays a crucial role in many physiological processes. Abnormal levels of tryptophan can be harmful to living organisms. Accurate real-time monitoring of its concentration is vital for maintaining health. Since the organism can only obtain tryptophan through external intake, the development of fluorescent probes for detecting tryptophan in various environments holds significant value. In this study, a Zr-MOF was synthesized using Zr 4+ as the metal node, 1,2,4,5-benzenetetracarboxylic acid (H₄BTEC) and 4-carboxyphenylboronic acid (CPB) as organic ligands via a simple one-pot synthesis method, named UiO-66-COOH-B(OH)₂ (UBB). Subsequently, by virtue of the abundant carboxyl groups, UBB was post-synthetically modified with Eu 3+ to form UiO-66-COOH-B(OH)₂ @Eu 3+ (UBBE). UBB and UBBE were investigated as fluorescent probes for the detection of the Trp. UBBE probe demonstrated a significantly lower detection limit than UBB probe as 0.026 μM and 0.79 μM, respectively. Additionally, the UBBE probe exhibits good selectivity, stability, and other advantages, allowing successful detection in fetal bovine serum and various food samples.
研究领域:
生物化学
·
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2020
·
IF 5.339
Chao Xia,Yan Xu,Meng-Meng Cao,Yun-Peng Liu,Jin-Feng Xia,Dan-Yu Jiang,Guo-Hong Zhou,Rong-Jun Xie,Da-Feng Zhang,Hui-Li Li
In the present study, a kind of Eu(III) post-functionalized Zr(IV)-based metal-organic framework (UiO-66(COOH) 2 , Zr-MOF: Eu 3+ ) was synthesized and utilized as an independently luminescent probe for sensing bilirubin (BR) in human serum, a biomarker of jaundice hepatitis. It can be served as a turn-off fluorescent switch for BR because its red emission from Eu 3+ can be easily quenched by BR through a fluorescent resonant energy transfer (FRET) process between BR and its ligands, and as a result, BR is recognized successfully. Particularly, Zr-MOF: Eu 3+ has shown many appealing properties, such as high sensitivity, quick response (less than 1 min), broad response window (0–15 μM), and excellent selectivity. Most importantly, a kind of portable test paper based on Zr-MOF: Eu 3+ probe has been developed for directly assessing the level of BR in real human serum and further diagnosing bilirubin-related diseases via visually observing the luminescent color variation.
研究领域:
生物化学
·
医学诊断
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）