Journal of Rubber Research
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2024
·Vol. 27
·期 3
·489-499
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IF 1.2
Liu Yonggang,Hao Wei,Zhang Wentao,Liu Zhaogang,Li Shuai,Hu Yanhong,Wu Jinxiu
A novel accelerator, cerium cysteine (Ce-Cys) was synthesised by double replacement reaction. The complex was characterised by FT-IR analysis, thermal analysis, EDTA titration and elemental analysis, respectively, to determine that the molecular formula of the complex was Ce (Cys) 3 Cl 3 ·3H 2 O. Cure characteristics, crosslink density, mechanical properties and thermo-oxidative ageing of NR compounds were investigated. The research revealed that the vulcanisates containing Ce-Cys manifested high scorch time, outstanding mechanical properties, and better thermo-oxidative ageing properties. Furthermore, based on kinetic calculations, it was concluded that as the amount of Ce-Cys increased, the reactivity of the crosslink precursor increased while the activation energy decreased.
研究领域:
材料科学
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化学工程
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DOI
高影响因子
INTERNATIONAL ENDODONTIC JOURNAL
·
2019
·Vol. 53
·期 1
·72-83
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IF 3.331
T. Ning,J. Shao,X. Zhang,X. Luo,X. Huang,H. Wu,S. Xu,B. Wu,D. Ma
Aim To comparatively evaluate changes in the proliferation and mineralization abilities of dental pulp stem cells (DPSCs) from juvenile and adult rats in a lipopolysaccharide (LPS)-induced inflammatory microenvironment to provide a theoretical basis for the age-related differences observed in DPSCs during repair of inflammatory injuries. Methodology DPSCs were isolated from juvenile (JDPSCs) and adult rats (ADPSCs), and senescence-associated β-galactosidase staining was used to compare senescence between JDPSCs and ADPSCs. Effects of LPS on JDPSCs and ADPSCs proliferation were investigated by cell counting kit-8 assays and flow cytometry. Alizarin red staining, quantitative reverse transcription polymerase chain reaction and Western blot assay were used to examine the effects of LPS on mineralization-related genes and proteins in JDPSCs and ADPSCs. Immunohistochemistry was used to compare interleukin-1β (IL-1β) and osteocalcin (OCN) expression in the pulpitis model. Unpaired Student's t -tests and one-way anova were used for statistical analysis. Results DPSCs were isolated from juvenile and adult rat dental pulp tissues. At low concentrations (0.1–1 μg mL −1 ), LPS significantly promoted the proliferation of JDPSCs ( P < 0.01) and ADPSCs ( P < 0.01 or P < 0.05), with the effect being stronger in JDPSCs than in ADPSCs. In addition, mineralized nodules and the expression of mineralization-related genes ( OCN , DSPP , ALP , BSP ) increased significantly after stimulation with LPS (0.5 μg mL −1 ) in JDPSCs and ADPSCs ( P < 0.01 or P < 0.05), and JDPSCs displayed a more obvious increase than ADPSCs. Western blots revealed OCN and ALP expression levels in JDPSCs treated with LPS were significantly upregulated ( P < 0.05); meanwhile, ALP expression in ADPSCs increased slightly but significantly ( P < 0.05), and OCN expression was not affected. Finally, IL-1β expression was significantly higher ( P < 0.05) and OCN expression was significantly lower ( P < 0.05) in the inflamed dental pulp of adult rats than in juvenile rats. Conclusions A certain degree of inflammatory stimulation promoted the proliferation and mineralization of DPSCs; however, this effect declined with age. The DPSCs of adult donors in an inflammatory microenvironment have a weaker repair ability than that of juvenile donors, who are better candidates for tissues damage repair.
研究领域:
牙科学
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干细胞生物学
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炎症
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细胞老化
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DOI
PubMed
高影响因子
BIOGERONTOLOGY
·
2025
·Vol. 26
·期 5
·1-24
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IF 4.1
Chen Wendi,Liu Shuang,Xu Guoqiang,Liu Xin,Shi Yuxuan,Wang Guolong,Ning Yunna,Lu Zhiming,Cao Yongzhi,Zhao Yueran
Zuogui pill (ZGP) and Yougui pill (YGP) are classical kidney-tonifying formulas in Traditional Chinese Medicine, widely used clinically but with their potential to delay ageing and improve ageing biomarkers remaining unclear. This study combined network pharmacology and Caenorhabditis elegans models to investigate the anti-ageing effects and mechanisms of ZGP and YGP. Both formulas significantly extended lifespan (ZGP dose-dependently at 5–20 mg/mL; YGP at 20 mg/mL) and improved ageing biomarkers, as evidenced by enhanced motility, reduced lipofuscin accumulation and endogenous ROS levels, and increased resistance to heat and oxidative stress. Network analysis identified quercetin and kaempferol as the top-ranked shared active components. Subsequent experimental validation demonstrated that kaempferol (0.05–0.2 mM) replicated these pro-longevity effects and was shown to act by inducing mitophagy: it triggered an initial decrease followed by a long-term increase in mitochondrial content, concomitant with upregulated expression of mitophagy genes. Crucially, the lifespan-extending effects of kaempferol, ZGP, and YGP were completely abolished in bec-1 and pink-1 null mutants. This study establishes that ZGP and YGP delay ageing and improve ageing biomarkers in C. elegans by activating BEC-1/PINK-1-dependent mitophagy. Kaempferol was identified as a major active component mediating this effect, highlighting a key mechanism for the pro-longevity properties of these traditional formulas.
研究领域:
中医
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药理学
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衰老研究
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分子生物学
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线粒体生物学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2019
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IF 5.399
Lin Zhu,Xinlei Wang,Xuebo Song,Fuping Zheng,Hehe Li,Feng Chen,Yuhang Zhang,Fuyan Zhang
Changes of key odorants and aroma profiles of Chinese Laowuzeng baijiu during its one-year ageing were determined by HS-SPME–AEDA and direct injection–AEDA (DI–AEDA). Ethyl hexanoate, ( E,E )-2,4-decadienal, and 2-phenylethyl acetate showed the highest FD value (486) in all ageing stages. With regards to aroma profiles, fruity, floral, acidic, sweet/honey and cheesy aromas were enhanced during storage, while pickled vegetable, grain and alcoholic notes weakened during the ageing. Quantitation and OAVs showed that most of the aroma compounds (OAVs > 1), including ethyl esters, aldehydes, and acids, increased their contents within the same period, whereas nonanal, 2-phenylethyl acetate, ethyl benzoate, 4-ethylguaiacol, propanol and 3-methyl-1-butanol decreased in content after the storage of 365 days. Simulated aged samples, in which fresh samples were spiked with 18 compounds, were examined by triangle tests, which indicated that the “fruity” compounds were crucial for maintaining the special aroma profile of an aged sample.
研究领域:
食品科学
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化学
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DOI
PubMed
高影响因子
POLYMER DEGRADATION AND STABILITY
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2018
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IF 3.193
Hao Yang,Hui Zhang,Henglun Cheng,Baoqing Shentu
Nano-TiO 2 immobilized antioxidant (TiO 2 -KH550-AG) was prepared to improve the ageing resistance of thermoplastic vulcanizate (TPV) exposed to UV/O 3 . TiO 2 nanoparticles were firstly modified with 3-aminopropyltriethoxysilane (KH550) and then reacted with the antioxidant , 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionic acid (AG), through the acyl chloride reaction followed by the amidation. FT-IR, XPS and TGA measurements confirmed that the antioxidant (AG) was chemically immobilized onto the nano-TiO 2 surfaces. TPV/TiO 2 -KH550-AG nanocomposites were prepared by melt mixing. SEM observation showed that TiO 2 -KH550-AG was homogeneously dispersed into the matrix of TPV. The UV/O 3 ageing test of the samples was carried out in the environment with constant UV intensity of 1.40 mW/cm 2 and ozone concentration of 100 ± 4 mg/m 3 . The ageing resistance behavior of the TPV/TiO 2 -KH550-AG nanocomposites was evaluated by the changes of surface morphology , mechanical properties and carbonyl index during the UV/O 3 ageing test. It was observed that the TPV/TiO 2 -KH550-AG exhibited high stability during the process of UV/O 3 ageing. The results revealed that the anti-ageing efficiency of TiO 2 -KH550-AG was superior to the mixture of the corresponding AG and modified TiO 2 , owing to the better dispersion and migration resistance of TiO 2 -KH550-AG.
研究领域:
材料科学
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高分子化学
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DOI
高影响因子
Journal of Crohns & Colitis
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2023
·Vol. 17
·期 8
·1278-1290
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IF 8
Hu Xiaoming,Jiao Fuxin,Deng Jiali,Zhou Ziheng,Chen Shanghai,Liu Changqin,Liu Zhanju,Guo Feifan
Background and AimsThe incidence of inflammatory bowel disease [IBD] in the elderly has increased in recent years. However, the mechanisms underlying the ageing-related IBD susceptibility remain elusive. Cytokine-inducible SH2-containing protein [CISH] is involved in regulating metabolism, the expansion of intestinal tuft cells and type-2 innate lymphoid cells, and ageing-related airway inflammation. Here, we investigated the role of CISH in ageing-related colitis susceptibility.MethodsCISH and phosphorylated signal transducer and activator of transcription-3 [p-STAT3] levels were evaluated in the colons of ageing mice and older ulcerative colitis [UC] patients. Mice with intestinal epithelial cell-specific knockout of Cish [CishΔIEC] and Cish-floxed mice were administered dextran sodium sulphate [DSS] or trinitrobenzene sulphonic acid [TNBS] to induce colitis. Colonic tissues were analysed in quantitative real-time polymerase chain reaction, immunoblotting, immunohistochemical, and histological staining experiments. Differentially expressed genes from colonic epithelia were analysed by RNA sequencing.ResultsAgeing increased the severity of DSS-induced colitis and the expression of colonic epithelial CISH in mice. CishΔIEC prevented DSS- or TNBS-induced colitis in middle-aged mice but not in young mice. RNA-sequencing analysis revealed that CishΔIEC significantly suppressed DSS-induced oxidative stress and proinflammatory responses. During ageing in the CCD841 cell model, knockdown of CISH decreased ageing-induced oxidative stress and proinflammatory responses, whereas these effects were compromised by knocking down or inhibiting STAT3. The increase in CISH expression was higher in the colonic mucosa of older patients with UC than in that of healthy controls.ConclusionsCISH might be a proinflammatory regulator in ageing; therefore, targeted therapy against CISH may provide a novel strategy for treating ageing-related IBD.
研究领域:
免疫学
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胃肠病学
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分子生物学
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衰老研究
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DOI
PubMed
高影响因子
Signal Transduction and Targeted Therapy
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2023
·Vol. 8
·期 1
·1-17
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IF 39.3
Liu Yi,Ji Yanhong,Jiang Ruiyi,Fang Chao,Shi Gang,Cheng Lin,Zuo Yinan,Ye Yixin,Su Xiaolan,Li Junshu,Wang Huiling,Wang Yuan,Lin Yi,Dai Lei,Zhang Shuang,Deng Hongxin
Cancer and impaired tissue wound healing with ageing are closely related to the quality of life of the elderly population. Given the increased incidence of cancer and the population ageing trend globally, it is very important to explore how ageing impairs tissue wound healing and spontaneous cancer. In a murine model of DSS-induced acute colitis and AOM/DSS-induced colitis-associated cancer (CAC), we found ageing significantly decreases intestinal wound healing and simultaneous CAC initiation, although ageing does not affect the incidence of AOM-induced, sporadic non-inflammatory CRC. Mechanistically, reduced fibroblasts were observed in the colitis microenvironment of ageing mice. Through conditional lineage tracing, an important source of fibroblasts potentially derived from intestinal smooth muscle cells (ISMCs) was identified orchestrating intestinal wound healing and CAC initiation in young mice. However, the number of transformed fibroblasts from ISMCs significantly decreased in ageing mice, accompanied by decreased intestinal wound healing and decreased CAC initiation. ISMCs-fibroblasts transformation in young mice and reduction of this transformation in ageing mice were also confirmed by ex-vivo intestinal muscular layer culture experiments. We further found that activation of YAP/TAZ in ISMCs is required for the transformation of ISMCs into fibroblasts. Meanwhile, the reduction of YAP/TAZ activation in ISMCs during intestinal wound healing was observed in ageing mice. Conditional knockdown of YAP/TAZ in ISMCs of young mice results in reduced fibroblasts in the colitis microenvironment, decreased intestinal wound healing and decreased CAC initiation, similar to the phenotype of ageing mice. In addition, the data from intestine samples derived from inflammatory bowel disease (IBD) patients show that activation of YAP/TAZ also occurs in ISMCs from these patients. Collectively, our work reveals an important role of the ageing stromal microenvironment in intestinal wound healing and CAC initiation. Furthermore, our work also identified a potential source of fibroblasts involved in colitis and CAC.
研究领域:
胃肠病学
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肿瘤学
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老年医学
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细胞生物学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2023
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IF 4.3
Qu Li,Haiyue Xu,Yougui Yu,Qing Zheng
The effect of acid–ester equilibrium on the quality of aged distilled liquor (e.g., Baijiu) and techniques for the rational design of flavor are yet to be fully understood. In terms of thermodynamics, the long-term ageing is a spontaneous process in which the thermodynamically unstable state changes to the stable state. This study used gas chromatography , sensory evaluation, and thermodynamic strategies to explore the acid–ester equilibrium during the ageing process of Baijiu. The content of most esters in new Baijiu is significantly higher than that of acids and a consistent rule in the ester balance was detected, with the reaction quotients of esterification reactions gradually approaching the equilibrium constants . The thermodynamic data confirm that the contents of esters and acids become stable after ageing. And the stability of ester and acid content is the premise of the soft and harmonious flavor of Baijiu: after ageing, the Baijiu will keep its harmonious flavor which derives from the blending process. On the contrary, the blended but unaged Baijiu, will lose the harmonious flavor due to the change of the content of esters and acids.
研究领域:
食品科学
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化学
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DOI
高影响因子
CONSTRUCTION AND BUILDING MATERIALS
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2025
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IF 8
Shun Chen,Yiming Cao,Xingyang He,Ying Su,Yingjie Wang,Wentian Wang,Chao Yang,Zhihao Jin,Meng Fan,Zhihao Liu,Haoyu Zhang
Although the effectiveness of different types and morphologies of polymers in improving asphalt performance varies significantly, it offers a pathway to achieve high-performance and durable modified asphalt. In current asphalt modification research, recycled polymers are one of the leading candidates. It helps alleviate environmental risks associated with polymer waste and enables the transformation of waste materials into valuable resources for producing high-performance modified asphalt. However, the inherent surface inertness of recycled polymers poses a significant challenge, negatively impacting the durability of modified asphalt in applications. Therefore, further research is needed to explore how surface modification and structural design of recycled polypropylene fibers can enhance the performance of modified asphalt. This study adopts a Schiff base reaction combined with an in-situ design approach to preparing heterostructured copper oxalate nanoparticles/recycled polypropylene fibers (RP@PDA-PEI@CO) and high-performance composite SBS-modified asphalt. Experimental results demonstrated that recycled polypropylene fibers modified via the Schiff base reaction (RP@PDA-PEI) significantly improved the rheological properties of the asphalt. Specifically, the storage modulus (G'), loss modulus (G′'), and rutting resistance parameter (G*/sinδ) at 55°C increased by 77.93 %, 72.42 %, and 74.46 %, respectively, compared to unmodified recycled polypropylene fibers (RP). The combination of Schiff base reaction and in-situ design (RP@PDA-PEI@CO) significantly enhanced the ageing resistance of the modified asphalt. Compared to RP, the carbonyl content was reduced by approximately 50.43 % after short-term ageing and 36.8 % after long-term ageing. The Schiff base reaction and in-situ design strategy activated the inert interface of recycled materials and improved the overall performance of modified asphalt. These findings provide an innovative design concept for preparing and applying advanced modified asphalt, offering a sustainable and high-performance solution for the construction industry.
研究领域:
土木工程
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材料科学
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DOI
高影响因子
Journal of Environmental Sciences
·
2020
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IF 4.302
Guanghuan Cheng,Han Liu,Tailu Dong,Qiuyu Li,Mingyang Sun,Liping Lou
Black carbon (BC) is a promising sediment amendment, as proven by its considerable adsorption capacity for hydrophobic organic pollutants and accessibility, but its reliability when used for the removal of pollutants in natural sediments still needs to be evaluated. For example, the ageing process, resulting in changing of surface physicochemical properties of BC, will decrease the adsorption capacity and performance of BC when applied to sediment pollution control. In this study, how the ageing process and BC proportion affect the adsorption capacity of BC-sediment systems was modelled and quantitatively investigated to predict their adsorption capacity under different ageing times and BC additions. The results showed that the ageing process decreased the adsorption capacity of both BC-sediment systems, due to the blockage of the non-linear adsorption sites of BC. The adsorption capacity of rice straw black carbon (RC)-sediment systems was higher than that of fly ash black carbon (FC)-sediment systems, indicating that RC is more efficient than FC for nonylphenol (NP) pollution control in sediment. The newly established model for the prediction of adsorption capacity fits the experimental data appropriately and yields acceptable predictions, especially when based on parameters from the Freundlich model. However, to fully reflect the influence of the ageing process on BC-sediment systems and make more precise predictions, it is recommended that future work considering more factors and conditions, such as modelling of the correlation between the adsorption capacity and the pore volume or specific surface area of BC, be applied to build an accurate and sound model.
研究领域:
环境科学
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污染控制
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DOI
PubMed
高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2018
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IF 4.841
Jia Nan Zhang,Bo Zhi Chen,Mohammad Ashfaq,Xiao Peng Zhang,Xin Dong Guo
Hyaluronic acid (HA) has many applications in human medicine and cosmetic industry. This study describes the fabrication and evaluation of cross-linked HA (cHA) based microneedles (HA-cHA-MNs) for anti-ageing treatment. The results suggested that the increasing content of cHA gel particles decrease the mechanical properties of MNs. The in-vitro degradation profile and in-vivo epidermal expansion in mice were also carried out. The data suggested that the proportion of cHA particles decreased the rate of MNs degradation. The prepared HA-cHA-MNs have prolonged effectiveness with high swelling retention time or epidermal expansion time in mice up to 6 days or more.
研究领域:
生物医学工程
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药剂学
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皮肤科学
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DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2019
·Vol. 30
·期 21
·19598-19608
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IF 2.195
Li Pengfei,Zhang Hong,Gao Caiyun,Jiang Guoxiang,Li Zhicheng
N egative temperature coefficient (NTC) ceramics based on simple ZnO were prepared and investigated in present work, where slight amount of Al- and La- ions were added as semiconductor dopants, and Cu-ions were selected as the sintering agent and property modificator. All the prepared ZnO-based ceramics have a wurtzite structure and show typical NTC characteristic. For various contents of Al-, La- and Cu- ions in ZnO-based ceramics, ρ 25 can be adjusted from 0.65 kΩ cm to 3.28 MΩ cm, and B values were obtained in range of 2500 K to 5850 K. The ageing treatment at 125 °C for 600 h in air revealed that the ZnO-based ceramics have high thermal stability with the resistance shift < 2%. The complex impedance spectra were applied to analyze the electrical performance.
研究领域:
材料科学
·
电子工程
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DOI
高影响因子
危险品化学品经营许可证（带存储）