Journal of Applied Spectroscopy
·
2014
·Vol. 81
·期 5
·807-811
·
IF 0.514
Liu Y.,Fan J.,Ma W.,Fu Ch.
An aqueous two-phase system of acetone and (NH 4 ) 2 SO 4 mixture has been developed for the extractive separation of copper (Cu). The complex formed by Cu(II) and Nuclear Fast Red was found to be extracted into the acetone-rich upper phase, and the absorbance of the complex was determined at 465 nm. Beer’s law was obeyed over the concentration range of ~0.6–3.2 mg/l for Cu(II) with a good linearity. The proposed method has been applied to the separation and determination of Cu(II) in seafood samples with a coefficient of variation less than 2.8%. The recovery obtained was ~96.3–101.7%.
研究领域:
分析化学
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环境化学
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食品安全检测
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DOI
高影响因子
PROCESS BIOCHEMISTRY
·
2020
·
IF 2.952
Yan Zhang,Hongzhou Zhang,Dong He,Xuejun Cao,Junfen Wan
Aqueous two-phase systems have been widely studied for the separation and recovery of bioproducts. In this study, the thermosensitive polymer EO 30 PO 70 and pH-responsive polymers (P ADB4.66 and P ADB6.26 ) were used to develop a recyclable aqueous two-phase system suitable for spiramycin partitioning. EO 30 PO 70 represents poly-(ethylene oxide-propylene oxide), where 30 and 70 indicate the proportions of ethylene oxide and propylene oxide, respectively. P ADB4.66 and P ADB6.26 represent poly-(acrylic acid-dimethylamine ethyl methacrylate-butyl methacrylate), where the numbers (4.66 and 6.26) in the subscripts indicate the isoelectric point of the polymers. The phase separation mechanism of ATPS was studied through low-field nuclear magnetic resonance (LF-NMR) and surface tension. Moreover, the parameters affecting spiramycin partitioning, such as system components and composition, pH, temperature, and type and concentration of additive salt, were investigated. The best partitioning conditions were determined using 4.70% P ADB6.26 and 31.67% EO 30 PO 70 in the presence of 0.05 mol/L NaSCN at 35 °C and pH = 9.2. The highest partition coefficient of spiramycin from fermentation broth was 11.92, with an extraction recovery of 97.69%. In addition, phase-forming polymers P ADB6.26 and EO 30 PO 70 could be recovered by regulating the pH or temperature, and their recoveries reached 92.39% and 98.76%, respectively.
研究领域:
生物化学
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分离科学
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制药工程
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DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Helin Xu,Xueqin Li,Yanyan Hao,Xiaobin Zhao,Yun Cheng,Jinli Zhang
To achieve simple and efficient separation of acteoside (ACT), extraction ACT from crude extracts of Cistanche tubulosa via ionic liquid (IL) based aqueous two-phase system (ATPS) is investigated. The IL–ATPS comprising [C 4 mim]BF 4 and (NH 4 ) 2 SO 4 exhibits excellent extraction performance, extracting almost all ACT in the [C 4 mim]BF 4 -rich phase. The high polarity of [C 4 mim]BF 4 having multi-hydrogen bond receptors is conducive for extracting ACT in the IL-rich phase, and (NH 4 ) 2 SO 4 not only provides a weak acidic microenvironment for increasing ACT stability in the IL-rich phase but also reduces the solubility of ACT in the salt-rich phase via the salting-out effect. In addition, molecular simulation results show that ACT is extracted in the [C 4 mim]BF 4 -rich phase via multi-interactions, including hydrogen bonding, van der Waals forces, and π–π stacking. This study is expected to provide a valuable reference for the separation of bioactive constituents from natural products.
研究领域:
植物化学
·
天然产物分离
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DOI
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Yun-Kun Zhu,Mei-Hui Li,Min Yang,Jiang-Xia Xu,Meng-Yao Ren,Zhong-kai Zhao,Liang Wang,Jie Yang,Jun Liu,Min-Wei Zhang
Walnut protein (WP) exhibits high nutritional value and excellent techno-functional properties, making it a promising candidate for broad applications in the food industry. It is commonly extracted from the meal after oil processing of walnut through alkali extraction with isoelectric precipitation (AE-IP). In this study, an aqueous two-phase system (DES-ATPS) consisting of betaine-urea/K 2 HPO 4 was used for the extraction of WP from walnut kernels and cold-pressed walnut meal. Comparison with AE-IP, WP extracted through DES-ATPS exhibited enhanced color characteristics and there was no significant difference in protein subunit composition. The WP extracted by DES-ATPS displayed lower Surface hydrophobicity (H 0 ) and intrinsic fluorescence intensities. Conversely, the exposure of internal groups in WP extracted by AE-IP resulted in a higher Zeta potential and a decrease in protein particle size. The comparative analysis revealed fundamentally distinct structural impacts between the two extraction methodologies. Notably, DES-ATPS-isolated WP demonstrated superior solubility under acidic conditions (pH 2).
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2021
·Vol. 193
·期 11
·3753-3764
·
IF 2.926
Chen Xiaochun,Huang Xiaoquan,Tang Yiwen,Zhang Lei
Nuclease P1 (NP1) can hydrolyze nucleic acids into four 5′-mononucleotides, which are widely used in the pharmaceutical and food industries. In this paper, an aqueous two-phase system (ATPS) was developed to purify NP1 from Penicillium citrinum . Polyethylene glycol (PEG) and nucleotides salts were studied to form ATPSs, among which PEG3000/disodium guanosine monophosphate (GMPNa 2 ) was researched, including the phase composition and pH. Using 14% (w/w) PEG3000 and 20% (w/w) GMPNa 2 ATPS at pH 5.0, the best recovery and purification factor, 82.4% and 3.59, were obtained. The recovery of NP1 was 98.3% by the separation of ultrafiltration from the PEG-rich phase. The recycling use of GMPNa 2 was also studied, and 95.1% of GMPNa 2 in the salt-rich phase was obtained with the addition of ethanol as the solvent. These results showed that the ATPS was effective for purification of NP1.
研究领域:
生物化学
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工业微生物学
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产品关联
DOI
PubMed
高影响因子
HYDROMETALLURGY
·
2019
·
IF 3.465
Tingting Sun,Yajing Pan,Xiao Sun,Yongqiang Zhang
An environmentally friendly approach was reported for recovering vanadium (V) from aqueous media with aqueous two-phase system (ATPS) formed by poly (ethylene glycol) 2000 (PEG2000) and sodium sulfate with octadecyl amine ethoxylate ethers as extractants. The effect of aqueous pH, the type of extractants and phase-forming salts, vanadium concentration and extracting temperature on the extraction performance of vanadium was investigated. The researched results indicated that the extraction rate of vanadium has remarkable relationship with vanadium species in aqueous phase, and the selected extractants can extract efficiently vanadium from salt-rich to PEG200-rich phase. The initial and equilibrium pH in aqueous phase has no relationship with vanadium concentration, and there is no new covalent bond generation between polyvanadate anion and extractant in PEG 2000-rich phase by FTIR, which can be deduced that polyvanadate anion is extracted into PEG 2000-rich phase due to the electrostatic attraction interaction between protonated extractant and polyvanadate anion. Adding chloride and nitrate in aqueous two-phase system could remarkably decrease the extraction rate of vanadium, and then sulfate is in favor of extracting vanadium. The extraction rate of vanadium decreases with increasing temperature from 40.0 °C to 60.0 °C, which showed the phase transfer of vanadium from salt-rich phase to PEG2000-rich phase is exothermal, resulting from the difference of interaction energy among all compositions in aqueous two-phase system. The vanadium can be easily separated out from the solution containing Fe(III), Co(II), Ni(II), Cu(II), Zn (II), Al(III), Cr(III) and Mn(II) under the suitable conditions. The stripping rate and precipitation rate of vanadium can be easily achieved to 90% and 80%, respectively, in single stage stripping with ammonium carbonate solution as stripping agent and phase-forming salt.
研究领域:
环境科学
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化学工程
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产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2021
·
IF 6.165
Xiaoxing Lu,Zhizhuo Lu,Rong Zhang,Lijiang Zhao,Hujun Xie
In this work, phase behaviors and pigment extraction performance of the ionic liquid-based aqueous two-phase system (IL-ATPS) formed by 1-ethylpiperazinium tertrafluoroborate ([C 2 pi][BF 4 ]) + sodium dodecyl sulfonate (SDS) and 1-phenylpiperazinium tertrafluoroborate ([Phpi][BF 4 ]) + sodium dodecyl benzene sulfonate (SDBS) are investigated. Phase composition of ATPS is determined, revealing ion exchange between conjugated phases. Mixing the piperazinium-based IL with the anionic surfactant produces a weakly acidic environment of ATPS which is suitable for the separation of curcumin . The influences of surfactant/IL mass ratio, pigment concentration and temperature on extraction efficiency and distribution coefficient are evaluated. The separation mechanism of ATPS is further explored by DLS, TEM and FTIR. The packing effect induced by the re-assembly of micelles formed mainly by IL cations and surfactant anions are the driving force for the preferable separation of curcumin, while carmine distributes in both phases with similar concentrations due to its high water solubility.
研究领域:
化学
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分离科学
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材料科学
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DOI
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2016
·Vol. 32
·期 4
·630-633
·
IF 1.086
Zhu Jian,Jin Zhimin
After being hydrolyzed into corresponding hydrosoluble carboxylate by 1,1,3,3,-tetramethylguanidine (TMG), camptothecin was extracted via the aqueous two-phase systems composed of ionic liquid 1,1,3,3-tetramethylguanidinium(TMGM) carboxylate and K 2 CO 3 , and was regenerated at the final by dilute aqueous hydrochloric acid. Among the ionic liquids, TMGM benzoate showed the best effect, TMGM formate the worst, this results were consistent with the liposolubilities of different carboxylic acid components in the ionic liquids. Through two-step extraction of TMGM benzoate ionic liquid/K 2 CO 3 aqueous two-phase at a favorable condition, camptothecin was obtained in a purity of 98.3% and a total yield of 85.1%, from the crude camptothecin extract with a purity of 14.2%.
研究领域:
药学
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分离科学
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产品关联
DOI
高影响因子
PROCESS BIOCHEMISTRY
·
2017
·
IF 2.497
Hongxia Pan,Ting Yang,Jialing Chen,Xuejun Cao
Cefprozil, a semi-synthetic antibiotic, has been used in pediatric therapy. The purpose of this study was to improve the yield of cefprozil and obtain purer product in an aqueous two-phase system composed of P ADB and P MDB . P ADB and P MDB were synthesized using acrylic acid, methacrylic acid, 2-dimethylaminoethyl methacrylate, and butyl methacrylate. Cefprozil was synthesized with 7-amino-3-(1-propenyl)-4-cephalosporanic acid (7-APRA) as the main ring, ( R )-amino-(4-hydroxyphenyl) acetic acid methyl ester hydrochloride (D-HPGME-HCl) as the acyl donor and the immobilized penicillin acylase as the catalyst. 7-APRA and D-HPGME-HCl were concentrated in the P MDB -rich phase, while cefprozil was in the P ADB -rich phase. The optimal partitiona coefficient of cefprozil was 1.84 with 40 mM KSCN, while the enzyme reaction yield of cefprozil reached 99.39% compared with 77.61% in the aqueous-phase system. The recovery of the two copolymers was up to 96.01% at pH 3.54. After crystallization, the purity of the product reached 88.02%, and no copolymer remained in the product. The combination of cefprozil∙DMF was detected by gas chromatography-mass spectrometry, FT-IR spectroscopy, thermogravimetric analysis, and electrospray ionization mass spectrometry. The results showed that no chemical bond existed between cefprozil and DMF.
研究领域:
药物化学
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生物工程
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制药工程
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产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Zhenyang Jiang,Shaojie Zhao,Minke Yang,Mingyue Song,Juan Li,Jinkai Zheng
Microcapsule colloidal systems have been widely used as delivery vehicles. Natural biopolymers are highly biocompatible shell materials for the fabrication of microcapsules, but no single substance can fulfill the requirements of aqueous two-phase interfacial assembly. In this study, we fabricated pectin-chitosan-collagen composite microcapsules (PCCMs) in an aqueous two-phase system to improve the robustness and sustained-release capacity of resultant microcapsules. Fourier transform infrared spectra and isothermal titration calorimetry thermodynamics analysis indicated that the PCCM interface can be stabilized by pectin, chitosan, and collagen via intermolecular electrostatic interactions and hydrogen bonds. The swelling experiment and atomic force microscopy nanoindentation measurements demonstrated that the PCCMs had excellent anti-swelling performance and enhanced mechanical strength of the composite interfacial membrane. Contraction-recovery measurements and scanning electron microscopy indicated that the PCCMs exhibited prominent anti-shrinkage capacity and restorable surface smoothness. The release kinetics of encapsulated substances under conditions of different osmotic pressure and salt concentrations revealed that the PCCMs had superior sustained-release capacity. This study yielded natural, biocompatible, and robust aqueous two-phase microcapsules for encapsulation and delivery of active biomacromolecules.
研究领域:
材料科学
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生物技术
·
药学
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产品关联
DOI
高影响因子
LANGMUIR
·
2019
·
IF 3.683
Jie Li,Feng Zhang,Leilei Jiang,Liang Yu,Lixiong Zhang
In the present work, a novel microchannel device was developed and used for the preparation of core–shell microspheres combining with a dextran/poly(ethylene glycol) diacrylate (DEX/PEGDA) aqueous two-phase system. Silica@silica core–shell microspheres were prepared as a model material. Silica@silica core–shell microspheres with different sizes of cores and thicknesses of shells were prepared by using different flowrate ratios of DEX/silica and PEGDA/silica aqueous solutions. The content of colloidal silica and the calcination temperature have a significant effect on the texture properties of the prepared core–shell microspheres. The surface area decreased from 199 to 177 m2/g with an increase in the colloidal silica content from 30 to 60 wt %. For a specific colloidal silica content (50 wt %), with the increase in calcination temperature from room temperature to 650 °C, the total pore volume went through a maximum of 0.7 cm3 g–1 with a surface area of 178 m2 g–1 and pore size of 7.32 nm at 450 °C. Due to the accumulation of metal nanoparticles in DEX, different metal nanoparticles (Ni and Pd) were successfully introduced into the core of the core–shell microspheres for the preparation of silica/metal nanoparticles@silica core–shell microsphere catalysts. The catalysts showed similar catalytic performance as the metal nanoparticles for hydrogenation of 4-nitrophenol with a conversion higher than 95%. However, the core–shell microsphere catalyst is much easier to recover. The reuse experiments indicated that the core–shell catalyst has high stability.
研究领域:
材料科学
·
化学工程
·
催化化学
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2022
·
IF 4.132
Haiming Huang,Xiaojing Mu,Jing Deng,Shangyou Xiao,Zhiwei Luo,Gang Chen
Aqueous two-phase system(s) (ATPS) has/have been widely employed in the extraction and separation of bioactive molecules from herbs due to its various advantages such as high efficiency and good selectivity. For selecting the type and amount of organics and salts in ATPS, onerous experimental trials are required to ensure the reliability. We intended to develop a theoretical method to select ATPS in the case of paeonol extraction from cortex moutan. The solvation free energies (Esolv) of paeonol in the top phase of 54 ATPS (ATPS-acetone, ATPS-acetone-EA, ATPS-THF, ATPS-THF-EA, ATPS-EtOH, and ATPS-EtOH-EA) were calculated with Gaussian 09, and the extraction yields with 54 ATPS were determined. By comparison of Esolv and yield, the Esolv rank was effective to select the optimal organic type and organic solvent fraction and aqueous salt concentration. With each series of 18 ATPS (ATPS-acetone plus ATPS-acetone-EA; ATPS-THF plus ATPS-THF-EA; or ATPS-EtOH plus ATPS-EtOH-EA), the paeonol yield was correlated with Esolv, suggesting that the optimal organic type and fraction and the aqueous NaH2PO4 concentration could be selected by using theoretical Esolv, or at least, the theoretical Esolv rank could offer effective guidance for experimental design, and thus, tedious and onerous experimental work for optimization in ATPS extraction can be significantly reduced.
研究领域:
生物化学
·
药学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）