INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xuan Li,Yixin Yun,Yitong Wang,Wanbin Zhu,Quan Bu,Yaxun Fan,Hongliang Wang
Inexpensive biomass materials hold great potential for the development of green delivery systems aimed at improving the extremely low utilization efficiency of pesticides. However, current systems face challenges in achieving both high encapsulation rates and drug loading capacities. This study introduces a novel method using chitosan (CS) and sodium lignosulfonate (SL) to co-assemble with avermectin (AVM), a widely used hydrophobic pesticide, forming AVM-CS-SL micro-nano capsules. Engineered under optimized conditions of pH 5 and 40 °C, the capsules exhibit an AVM encapsulation efficiency of 84.27 % and a loading capacity of 90 %. The AVM-CS-SL capsules demonstrate multifunctional attributes that enhance pesticide application. The capsules, with an average diameter of 356 nm, facilitate stable embedding in leaf grooves and enable effective adhesion to leaf surfaces, thereby improving their resistance to wash-off by rain compared to conventional formulations. Their core-shell structure protects AVM from photodegradation, ensuring long-term stability and efficacy. The capsules also exhibit enhanced bioactivity, with higher mortality rates in Plutella xylostella larvae and low genotoxicity to Vicia faba plants. These findings highlight the strategy of developing multifunctional delivery systems by the co-assembled carrier materials with active ingredients, offering an effective solution for the sustainable development of society and environment.
研究领域:
农业科学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Xiquan Ding,Qian Du,Fei Gao,Long Chen,Tingyu Wang,Fangyuan Chen,Zhanghua Zeng,Yan Wang,Haixin Cui,Bo Cui
Root-knot nematodes are globally prevalent plant-parasitic pests, distinguished by their widespread distribution, extensive host range, and challenging control measures. Avermectin B2, one of the few effective nematicides available, faces limitations in efficacy and poses environmental concerns due to its eco-unfriendly composition and poor utilization in traditional emulsifiable concentrate (EC) formulations. In this study, we developed core–shell structured Ave B2-BS@Zein@SC nanospheres, incorporating zein as the carrier, sodium caseinate as the stabilizer, and butyl stearate as the temperature “switch”. These nanospheres demonstrated multiresponsive characteristics to temperature, pH, and enzymatic conditions, facilitating rapid pesticide release under elevated temperatures, mildly acidic or alkaline conditions, and in the presence of protease. These controlled-release properties effectively responded to both environmental factors during nematode outbreaks and the intestinal conditions of the nematodes, enhancing pesticide targeting and utilization efficiency. The engineered nanospheres improved the mobility and stability of avermectin B2 in soil, enabling more comprehensive control of nematodes across broader and deeper soil layers. Additionally, the penetration capability of Ave B2-BS@Zein@SC into plant roots effectively eliminated nematodes that had already invaded the root system, thereby strengthening crop protection. Crucially, Ave B2-BS@Zein@SC demonstrated enhanced safety for nontarget organisms. This environmentally conscious, and multiresponsive pesticide nanodelivery system presents a promising strategy for sustainable subterranean pest management.
研究领域:
植物保护
·
农药科学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Shihao Gu,Mei Liu,Renle Xu,Xu Han,Yuhang Lou,Yue Kong,Yanqing Gao,Shibin Shang,Zhanqian Song,Jie Song,Jian Li
The exploration of environmentally friendly, less toxic, sustained-release insecticide is increasing with the growing demand for food to meet the requirements of the expanding population. As a sustained-release carrier, the unique, environmentally friendly intelligent responsive hydrogel system is an important factor in improving the efficiency of insecticide utilization and accurate release. In this study, we developed a facile approach for incorporating the natural compound rosin (dehydroabietic acid, DA) and zinc ions (Zn2+) into a poly(N-isopropylacrylamide) (PNIPAM) hydrogel network to construct a controlled-release hydrogel carrier (DA-PNIPAM-Zn2+). Then, the model insecticide avermectin (AVM) was encapsulated in the carrier at a drug loading rate of 36.32% to form AVM@DA-PNIPAM-Zn2+. Surprisingly, the smart controlled carrier exhibited environmental responsiveness, strongly enhanced mechanical properties, self-healing ability, hydrophobicity, and photostability to ensure a balance between environmental friendliness and the precision of the drug release. The release experiments showed that the carboxyl and amide groups in the polymer chains alter the intermolecular forces within the hydrogel meshes and ingredient diffusion by changing temperatures (25 and 40 °C) and pH values (5.8, 7.4, and 8.5), leading to different release behaviors. The insecticidal activity of the AVM@DA-PNIPAM-Zn2+ against oriental armyworms was good, with an effective minimum toxicity toward aquatic animals. Therefore, AVM@DA-PNIPAM-Zn2+ is an effective drug delivery system against oriental armyworms. We anticipate that this ecofriendly, sustainable, smart-response carrier may broaden the utilization rosin and its possible applications in the agricultural sector.
研究领域:
农业科学
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环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2021
·Vol. 77
·期 7
·3300-3312
·
IF 4.845
Long Chen,Yuanxiong Lin,Hongjun Zhou,Li Hao,Huayao Chen,Xinhua Zhou
BACKGROUND A large amount of pesticides that are not deposited on desired locations due to drift and rolling, endangering the ecological environment and human health. Therefore, it is urgent to develop environmentally friendly and foliar affinity formulations. The design and construction of pesticide nano-delivery system is considered to be an effective way to solve this problem. RESULTS In this research, polyamine-modified zein (AM-zein) was synthesized by incorporating ethylenediamine-terminated polyethyleneimine into zein to improve its stability as a nanocarrier, enhance electrostatic force between the carrier and pesticides and plant foliage. Avermectin (AVM)-loaded nanoparticles, containing a high positive charge, were prepared by the anti-solvent method using AM-zein as carrier. The nanoparticles can be stored for 30 days without any significant change in the particle size and stably dispersed at pH 5–9. Compared to the commercial emulsifiable concentrate (EC), nanoparticles dispersions exhibited better leaf affinity, and the retention of dispersion increased from 7.82 to 13.86 mg/cm 2 . Interestingly, we have discovered for the first time that the ultraviolet (UV) barrier effect of zein increases while prolonging the UV exposure time; 30.47% of the encapsulated AVM remained intact after exposure to UV for 60 min, compared to only 17.13% for the EC. Insecticidal activity of AVM nanoparticles did not improve compared to EC, but they demonstrated significantly lower toxicity against zebrafish. CONCLUSION This research opens up a new idea for improving the stability of zein nanoparticles, providing a novel path to deliver pesticides efficiently and eco-friendly. © 2021 Society of Chemical Industry.
研究领域:
农业科学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2021
·
IF 3.882
Kai Chen,Shengrong Yuan,Dan Wang,Yinli Liu,Fengfeng Chen,Dongming Qi
Avermectin (AVM) is a highly effective and safe biopesticide but is very sensitive to ultraviolet (UV) light and exhibits poor water solubility. Developing green and multifunctional adjuvants is important for the protection and controlled release of AVM. In this work, a number of water-soluble enzymatic hydrolysis lignins (W-EHLs) were prepared via grafting basic amino acids and used as emulsifiers with co-surfactants to prepare high-internal phase emulsions (HIPEs). The results showed that W-EHLs with co-surfactants could be prepared with HIPEs that contained 90 vol % green oil phases such as turpentine, and the stability of the HIPEs first increased and then decreased when the rate of grafting of basic amino acids on lignin increased from 0.26 to 1.46 mmol/g. The more polar oil droplets were less deformable due to their higher viscosity, thereby affording a stability advantage to HIPEs. Subsequently, the relations between the stability and interfacial viscoelasticity of the emulsion were effectively correlated by interfacial rheology, droplet size, and physical stability tests. The results showed that HIPEs with smaller droplets had poor fluidity and strong interfacial viscoelasticity due to their higher droplet packing density, which resulted in good macroscopic stability. Like the AVM carrier, the retention rate of AVM in HIPEs was 80.1% after UV radiation for 72 h, which represented the highest UV protection efficiency in AVM delivery systems. The release curves showed that the rate of release of AVM from HIPEs was adjusted by controlling the pH value of the medium. In addition, the release of HIPEs is completely in accord with both diffusion and the matrix erosion mechanism. The strategy could be extended to other sensitive pesticides and used to promote the development of sustainable agriculture.
研究领域:
植物学
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农业科学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Enzhuang Pan,Huizhen Chen,Xinyu Wu,Nana He,Jiajie Gan,Huimiao Feng,Yong Sun,Jingquan Dong
Avermectin pollution is an important problem that cannot be ignored in aquatic system in recent years. It has brought great trouble to freshwater aquaculture, especially fishery aquaculture. Plant-derived quercetin has anti-inflammatory and antioxidant properties and is widely used as a dietary additive, but its protective effect on immune damage induced by avermectin in freshwater carp remains unclear. This study evaluated the role of dietary additive quercetin supplementation in chronic avermectin exposure of carp spleen. Sixty carp were divided into 4 groups ( n = 15/ group), including control group, avermectin treatment group, quercetin treatment group, quercetin and avermectin co-treatment group. Carp were exposed to a 1/10 96 h LC 50 dose of avermectin for 30 d and fed a carp diet containing 400 mg/kg quercetin twice a day (3% body weigh/ carp). The results showed that chronic avermectin exposure caused the loose parenchymal structure of carp spleen tissue and the increase of inflammatory cells, accompanied by increased transcription levels of pro-inflammatory il-1β , il-6 , tnf-α and decreased levels of anti-inflammatory factors il-10 and tgf-β1 , ROS accumulation in spleen tissue. MDA content increased and T-AOC, CAT and GSH levels decreased. Quercetin down-regulates the NF-κB pathway by inhibiting the expression of iNOS and activating p38 MAPK, blocking the transcription of inflammatory factors, and alleviating the inflammation of carp spleen caused by chronic avermectin exposure. In addition, quercetin inhibits the over-activation of Nrf2/Keap-1 signaling axis, blocks ROS accumulation, and restores the spleen REDOX homeostasis. In conclusion, quercetin, as a dietary additive for carp feed, can effectively improve the immune damage caused by avermectin pollution in aquatic environment, resist spleen inflammation and oxidative stress, and provide a theoretical basis for clinical development of freshwater carp feed.
研究领域:
水产养殖
·
免疫学
·
毒理学
·
药理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yan-Jun Dong,Sai-Nan Guo,Jun Lu,Dan Wang,Hua Zou,Yuan Le,Jie-Xin Wang
Sustainable crop protection requires nano-pesticides that combine multi-stimuli-responsive release with excellent droplet deposition and spread. However, simultaneous realization of these two functions is hindered by expensive pesticide-modified composite materials and complex preparation technologies. Herein, by introducing inexpensive carboxymethylcellulose (CMC) with varied characteristics, avermectin nanopesticides (Av@CMC) with an average particle size of 158 nm were instantaneously and continuously synthesized via high-gravity anti-solvent precipitation method in a rotating packed bed (RPB). Compared with the conventional stirred tank, RPB sample has more regular morphology, smaller particle size, and higher drug loading capacity. More importantly, this method is easy to scale up, which may facilitate the large-scale synthesis of nanopesticides. Remarkably, the unique internal composition in CMC interacts with the micro/nanostructures of hydrophobic foliage surface, ensuring the complete deposition and spread of pesticide drops after high-position dripping. The reduced contact angle of Av@CMC (70°) compared with water (108°) confirms that CMC incorporation facilitates uniform surface wetting. Relative to technical avermectin, Av@CMC exhibits superior insecticidal efficacy against Plutella xylostella while maintaining no significant effect on non-target organism. This research presents a promising pesticide application strategy that improves agricultural productivity and supports sustainable agriculture.
研究领域:
农业化学
·
纳米技术
·
高分子科学
·
害虫管理
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Tong-fang Jing,Da-xia Zhang,Yan Jin,Guo-dong Si,Bei-xing Li,Wei Mu,Feng Liu
Sorption is the key process that regulates the dispersion of pesticides in the environment once they are introduced into the soil. However, the relationship between the molecular structures of these compounds and the sorption mechanism needs to be further clarified. Our objectives were to uncover sorption drivers in distinct soil samples for the parent avermectin B1a (–OH:3) and pharmacophore-altered derivatives [derivative-1 (–OH:2) and derivative-2 (–OH:1) series]. Avermectin B1a sorption to soil was extensive and essentially irreversible, with avermectin B1a exhibiting significantly greater sorption than the derivatives. The sorption difference between avermectin B1a and derivatives in soil suggested that –OH pharmacophore was the main sorption site for avermectin B1a in soil. Further sorption tests on avermectin B1a and derivatives were carried out on humic acid fractions (HA1 and HA2) that we extracted from the soil. Avermectin B1a sorbed most extensively to humic acid fractions, followed by derivative-1 and derivative-2, thereby indicating the –OH pharmacophore remains the main site for the sorption of avermectin B1a and derivatives by the humic acid fractions. The HA1 fraction showed significantly greater sorption of the compound than the HA2 fraction. Elemental analysis and 13 C NMR analysis demonstrated that the HA1 fraction contains more polar functional groups than the HA2 fraction. This suggests that the sorption of avermectin B1a and derivatives by the soil is due to the interaction between the –OH pharmacophore and the polar functional groups in the humic acid, implying that hydrogen bonding may be the main sorption mechanism. This study highlighted the potential sorption mechanisms of pharmacophores in pesticide compounds with humic acid fractions.
研究领域:
环境科学
·
土壤学
·
农药化学
✓ 已验证
产品关联
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Hairui Han,Nana He,Enzhuang Pan,Xuelian Tan,Zuwang Yang,Xueqing Li,Dahua Shi,Jingquan Dong
Avermectin (AVM) is a widely used insecticide. Due to its sensitive toxicity to aquatic organisms , the toxicology of AVM on fish intestines remains unclear. Here, we established a 96 h AVM acute toxicity model to explore the effects of AVM on the intestinal tract of carp . The 96 h LC 50 of carps exposed to AVM was 24.04 μg/L, 12.02 μg/L was selected as the high-dose group and 3.005 μg/L was selected as the low-dose group. After 96 h of exposure, intestinal tissues were collected and subsequently analyzed for histopathology, the activities of antioxidant oxidases (CAT, SOD , GSH-Px), and the expression of mRNA associated with oxidative stress , inflammation, and apoptosis. Our study showed that AVM exposure caused intestinal damage in carp, decreased the expression of the tight junction protein gene, activated oxidative stress, induced apoptosis, and induced intestinal inflammation in carp. Therefore, we demonstrated that AVM exposure compromised the integrity of the intestinal barrier in carp, activated oxidative stress, induced endogenous apoptosis, and induced intestinal inflammatory responses. These results indicate that AVM, as a drug-sensitive to aquatic organisms, has a much more complex toxic effect on the fish intestinal tract, which provides a new perspective for studying the toxicology of AVM on the fish intestinal tract.
研究领域:
水生毒理学
·
鱼类生理学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2023
·
IF 3.9
Mengyuan Zhou,Jingchao Qiang,Jiajie Gan,Xuhui Xu,Xing Li,Shuai Zhang,Baoshi Xu,Zibo Dong
One of the most often used insecticides is Avermectin (AVM), which is toxic to aquatic organisms. Quercetin (Que) is a fruit and vegetable rich flavonoid with antioxidant activity. Fish gills are the most important respiratory organ. Carp is a biological model for studying environmental toxicology. To better understand the mechanism by which Que reduces AVM-induced gill damage in carp, we created a carp model with AVM and Que supplementation. In this study, four groups were created: the Control group, the Que group (400 mg/kg), the AVM group (2.404 μg/L), and the Que plus AVM group. The analytical methods used in this study were pathological structure examination, QPCR, Reactive Oxygen Species (ROS) and Western blot. The results showed that Que alleviated AVM-induced oxidative stress, inflammatory damage and apoptosis in the gill tissues of carp by activating the Nrf2 pathway. The mechanism was that Que alleviated the accumulation of ROS, reduced the balance between oxidation and antioxidant disrupted by AVM exposure, lowered the content of lipid peroxidation product malondialdehyde (MDA), and increased the content of antioxidant enzymes glutathione (GSH) and catalase (CAT). The Nrf2 pathway was activated. Meanwhile, Que inhibited apoptosis in the gills of carp by decreasing the levels of Bax, Cytochrome C(Cytoc), Caspase9, Cleaved-Caspase3, and elevating Bax. This is significant for future studies on Que and AVM. New recommendations are provided to reduce the threat of pollution in the water environment.
研究领域:
环境毒理学
·
水生生物学
·
药理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2016
·Vol. 40
·期 2
·424-430
·
IF 2.741
Xiaoxiao You,Lei Gao,Dongli Qin,Ligang Chen
A novel and highly efficient approach to obtain magnetic molecularly imprinted polymers is described to detect avermectin in fish samples. The magnetic molecularly imprinted polymers were synthesized by surface imprinting polymerization using magnetic multiwalled carbon nanotubes as the support materials, atom transfer radical polymerization as the polymerization method, avermectin as template, acrylamide as functional monomer, and ethylene glycol dimethacrylate as crosslinker. The characteristics of the magnetic molecularly imprinted polymers were assessed by using transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, vibrating sample magnetometry, X-ray diffraction, and thermogravimetric analysis. The binding characteristics of magnetic molecularly imprinted polymers were researched through isothermal adsorption experiment, kinetics adsorption experiment, and the selectivity experiment. Coupled with ultra high performance liquid chromatography and tandem mass spectrometry, the extraction conditions of the magnetic molecularly imprinted polymers as adsorbents for avermectin were investigated in detail. The recovery of avermectin was 84.2–97.0%, and the limit of detection was 0.075 μg/kg. Relative standard deviations of intra- and inter-day precisions were in the range of 1.7–2.9% and 3.4–5.6%, respectively. The results demonstrated that the extraction method not only has high selectivity and accuracy, but also is convenient for the determination of avermectin in fish samples.
研究领域:
分析化学
·
环境与食品安全分析
·
高分子科学
·
分子印迹技术
✓ 已验证
DOI
高影响因子
AQUATIC TOXICOLOGY
·
2024
·
IF 4.1
Yue Xin,Xueqing Li,Kaixin Ping,Yannan Xiang,Mengxin Li,Xing Li,Haitao Yang,Jingquan Dong
Flavonoid quercetin (QUE) has biological activities of anti-oxidation, anti-inflammation and anti-apoptosis, however, its protective effects against avermectin (AVM) induced liver toxicity in carp remains unclear. The objective of this research is to explore the biologically potent effects of QUE in AVM-induced hepatotoxicity in carp and its underlying mechanism. Therefore, we established a liver injury model in carp induced by AVM to evaluate QUE against AVM induced liver toxicity in carp. In this investigation, AVM dosage was determined as 2.404 μg/L for both groups, and an experimentation of 30 days duration was carried out. Various methods including hematoxylin and eosin (H&E) staining, biochemical kits, real-time quantitative PCR (qRT-PCR), western blotting, TUNEL, reactive oxygen species (ROS) staining, immunofluorescence (Hoseinifar, et al.,), and oil red O staining were used in this study. Results showed that the growth inhibition of carp was relieved in the QUE treatment group comparing to the AVM group. In the QUE treatment group, there was a significant decrease in the levels of ALT and AST in carp liver tissue. Additionally, the histopathological damage and lipid accumulation were alleviated compared to the AVM group. Moreover, QUE prevented AVM induced decrease in the activities of antioxidant enzymes of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), glutathione (GSH), catalase (CAT) and the accumulation of reactive oxygen species (ROS), but reduced accumulation of malondialdehyde (MDA). In addition, the mRNA levels of liver pro-inflammatory factors of tumor necrosis factor-α (TNF-α), interleukin-1β (iL-1β), interleukin-6 (iL-6), interleukin-10 (iL-10) and the protein levels of NOD-like receptor protein 3 (NLRP3) inflammasome were significantly down-regulated in the QUE treatment group in comparison to the AVM group. We also found that QUE could affect the expression of Bcl2-associated x (Bax), B-cell lymphoma-2 (Bcl-2), cleaved-cysteinyl aspartate specific proteinase (C Caspase3) key apoptotic proteins and TUNEL-labeled apoptotic hepatocytes by regulating SIRT1/FOXO3a signal pathway. In summary, QUE alleviated the growth inhibition, liver oxidative damage, lipid accumulation, inflammatory response, and apoptosis of carp induced by AVM. QUE is a potential protective agent against liver injury induced by AVM in carp.
研究领域:
水产养殖
·
毒理学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）