Frontiers in Bioengineering and Biotechnology
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2025
·
IF 4.8
Qianqian Wang,Fang Ma,Ying Xian,Xingyan Shi,Shenglan Ma,Rui Zhang,Hualin Zhang
Introduction: Wound closure, infection prevention and accelerated healing are important to consider in the development of multifunctional dressings. In this study, we aimed to design a dressing that can simulate the structure of normal skin, prevent bacterial infection, improve hemostasis and angiogenesis, accelerate cell adhesion and proliferation, and continuously promote skin regeneration.Methods: We created a multifunctional bilayer dressing coloaded with nanosilver and basic fibroblast growth factor (bFGF) via emulsion electrospinning and vacuum freeze-drying methods. The upper hydrophobic layer was composed of a poly(lactic-co-glycolic acid)/wool keratin (PLGA/WK) electrospun membrane containing nanosilver and bFGF, and the lower hydrophilic layer was composed of a chitosan sponge containing bFGF.Results and Discussion: The bilayer dressing allowed the sustained release of nanosilver and bFGF for more than 2 weeks, had good water absorption and water retention rates, had an appropriate water vapor transmission rate, absorbed excess exudate, and maintained a moist wound environment. In vitro, the dressing prevented bacterial penetration, accelerated fibroblast migration, and promoted angiogenesis and coagulation. Furthermore, the bilayer dressing facilitated cell migration and proliferation, promoted vessel formation, shortened the healing time and significantly promoted wound healing in vivo. By day 3, the wound healing rate in the bilayer dressing group (35.28% ± 2.06%) was significantly higher than that in the control group (23.99% ± 4.32%), representing an increase of approximately 47%. Our findings suggest that this multifunctional bilayer dressing coloaded with nanosilver and bFGF is a potential candidate for skin repair and regeneration applications.
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DOI
PubMed
高影响因子
BURNS
·
2024
·
IF 3.2
Ning Liu,Baoxia Xue,Wei Cheng,Yong Liu,Mei Niu,Yongzhen Yang,Shiping Yu,Li Zhang
Dressings play a crucial role in the management of burn wound. In this study, the cotton bandage was modified with the Poly (N-isopropylacrylamide) / graphite oxide/nano silver (PNIPAM/GO-Ag) hydrogel to obtain a novel dressing (PNIPAM/GO-Ag/COT). The healing effect of the PNIPAM/GO-Ag/COT dressing on deep second-degree burn wounds in rats and the changes of related inflammatory factors were explored and analyzed systematically. The deep second-degree burn model was established by the steam scald method in Sprague–Dawley (SD) rats. The granulation tissue, collagen deposition, the expressions of tumor necrosis factor-α (TNF-α), and basic fibroblast growth factor (bFGF) of the wound were evaluated by means of the HE staining, Masson staining, ELISA, and immunohistochemistry methods. The results showed that, compared with the blank group (rats without the dressing treatment), the PNIPAM/GO-Ag/COT dressing reduced the expression of TNF-α by approximately 18 % and promoted the bFGF expression in wound tissue. Compared to the control group (rats with the gauze treatment), the wound healing rate in the PNIPAM/GO-Ag/COT dressing group was 58 % on the 14th day, with an increase of 30 %. These results demonstrated that the PNIPAM/GO-Ag/COT dressing primarily promoted burn wound healing by reducing inflammatory reactions, promoting collagen deposition, and enhancing the expression of bFGF.
研究领域:
生物医学工程
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伤口愈合
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材料科学
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药剂学
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DOI
PubMed
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2022
·
IF 3.322
Hao Li,Xue Gan,Lei Pan,Yuhua Zhang,Xintian Hu,Zhengbo Wang
Stem cell replacement therapy is considered a promising treatment for diseases of the central nervous system. Improving the ratio of surviving transplanted cells and the efficiency of differentiation into functional neuronal cells are the most important issues related to research on neuroregenerative medicine. Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) have been reported to promote the proliferation and differentiation of neural stem cells (NSCs) in vitro , but whether they have the same effect in vivo is unclear. In this study, NSCs derived from rhesus monkey embryonic stem cells (ESCs) were resuspended in medium with or without EGF/bFGF and xenotransplanted into the rat striatum. No behavioral abnormalities or teratoma formation were observed in the recipient engrafted rats. GFP-labeled cells exhibited a higher survival rate and longer migration in the EGF/bFGF group than control group at 2 months after transplantation. Moreover, the percentages of Tuj1 + neurons and Map2 + neurons in the EGF/bFGF group were significantly higher than those in the control group, while the percentages of astrocytes and oligodendrocytes were significantly lower in the EGF/bGFG group than control group. These findings indicated that EGF/bFGF can promote protrusion of nerve fibers and the survival and neuronal differentiation of transplanted NSCs in the recipient brain, suggesting that EGF/bFGF has a potential application for stem cell therapy .
研究领域:
神经科学
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再生医学
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细胞生物学
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DOI
PubMed
高影响因子
Materials Today Bio
·
2025
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IF 10.2
Meilin Yi,Wenzhang Jin,Haobing Li,Xiaoying Niu,Junru Wang,Shunfu Wang,Wa Zhang,Mengxuan Zhou,Zhe Wang,Yutong Zhou,Xuchen Deng,Jingyong Huang,Xiang Su
When the skin suffers from burns or frostbite, its normal structure and function are severely damaged, leading to problems such as inflammation, oxidative stress and difficulties in vascularization, which result in difficult wound healing. Currently, the existing treatment options have limitations in efficacy. The pH value of the burns and frostbite (B/F) wound microenvironment shows a dynamic change: it is acidic in the initial stage, gradually alkalizes as the repair process progresses, and then returns to a slightly acidic state in the later stage. Based on this, we designed a pH-responsive bilayer hydrogel with sequential release of morin-based nanoparticles and basic fibroblast growth factor (bFGF) for the treatment of B/F. 4-hydroxyphenylboronic acid pinacol ester (PAPE) modified fucoidan (Fu), chitosan (Cs) and morin self-assembled to form nanoparticles (CFMNPs), which exhibited excellent pH sensitivity. Then, using methacrylated hyaluronic acid (MeHA) as the main component, taking advantage of the concentration difference and the property that MeHA is easily degraded in an acidic environment, a 0.8% MeHA lower layer hydrogel loaded with CFMNPs and a 2% MeHA upper layer hydrogel loaded with bFGF were prepared. In vitro experiments confirmed the pH responsiveness and cytocompatibility of the bilayer hydrogel. In vivo experiments on mouse B/F wounds verified that the bilayer hydrogel can achieve sequential release of active ingredients according to the pH changes of the B/F wound, effectively inhibit inflammation, resist oxidative stress, promote new blood vessel formation, and ultimately accelerate wound healing. This provides a highly potential and efficient new strategy for the treatment of B/F.
研究领域:
生物医学工程
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伤口愈合
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药物递送系统
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生物材料
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组织工程
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纳米医学
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DOI
PubMed
高影响因子
Biomaterials Science
·
2022
·Vol. 10
·期 9
·2409-2416
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IF 7.59
Yonghang Chen,Weijiang Yu,Xuedan Qian,Xinfang Li,Youxiang Wang,Jian Ji
Infected wound healing is a complex and dynamic process affecting millions of people. Since wound healing contains multiple stages, it requires staged management to realize the early inhibition of infection and the subsequent promotion of wound healing. A key point is to design a biphasic release system with antibacterial agents and growth factors to promote wound regeneration. As a safe, efficient and painless transdermal drug delivery method, microneedles (MNs) have attracted widespread attention. Herein, we present dissolving MNs with the biphasic release of an antibacterial agent and a growth factor to promote wound healing. bFGF was first encapsulated in PLGA microspheres (bFGF@PLGA) and then co-loaded with free ofloxacin onto polyvinylpyrrolidone MNs. Owing to the fast dissolution of the substrate, ofloxacin was quickly released to rapidly inhibit infection, while the PLGA microspheres were left in the wound. Due to the slow degradation of PLGA, bFGF encapsulated in the PLGA microspheres was slowly released to further promote wound healing. In vivo studies demonstrated that the MNs with the biphasic release of antibacterial agent and growth factor exhibited a superior capability to promote wound healing. This biphasic release system combined with microneedles has a bright future in wound healing.
研究领域:
药学
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生物医学工程
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伤口愈合
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DOI
PubMed
高影响因子
Small
·
2024
·
IF 13
Sipin Zhu,Qiuji Wu,Yibo Ying,Yuqin Mao,Wenjie Lu,Jie Xu,Xiong Cai,Huacheng He,Jiang Wu
Spinal cord injury (SCI) is a severe clinical disease usually accompanied by activated glial scar, neuronal axon rupture, and disabled motor function. To mimic the microenvironment of the SCI injury site, a hydrogel system with a comparable mechanical property to the spinal cord is desirable. Therefore, a novel elastic bovine serum albumin (BSA) hydrogel is fabricated with excellent adhesive, injectable, and biocompatible properties. The hydrogel is used to deliver paclitaxel (PTX) together with basic fibroblast growth factor (bFGF) to inhibit glial scar formation as well as promote axon regeneration and motor function for SCI repair. Due to the specific interaction of BSA with both drugs, bFGF, and PTX can be controllably released from the hydrogel system to achieve an effective concentration at the wound site during the SCI regeneration process. Moreover, benefiting from the combination of PTX and bFGF, this bFGF/PTX@BSA system significantly aided axon repair by promoting the elongation of axons across the glial scar with reduced reactive astrocyte secretion. In addition, remarkable anti-apoptosis of nerve cells is evident with the bFGF/PTX@BSA system. Subsequently, this multi-functionalized drug system significantly improved the motor function of the rats after SCI. These results reveal that bFGF/PTX@BSA is an ideal functionalized material for nerve repair in SCI.
研究领域:
神经科学
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生物医学工程
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药学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2024
·
IF 5.4
Yang Xiang,Yuanhui Gao,Qiuhua Cheng,Zhongwen Lei,Xiaoyu Zhang,Yijun Yang,Jianquan Zhang
Bile duct injury presents a significant clinical challenge following hepatobiliary surgery, necessitating advancements in the repair of damaged bile ducts is a persistent issue in biliary surgery. 3D printed tubular scaffolds have emerged as a promising approach for the repair of ductal tissues, yet the development of scaffolds that balance exceptional mechanical properties with biocompatibility remains an ongoing challenge. This study introduces a novel, bio-fabricated bilayer bile duct scaffold using a 3D printing technique. The scaffold comprises an inner layer of polyethylene glycol diacrylate (PEGDA) to provide high mechanical strength, and an outer layer of biocompatible, methacryloylated recombinant collagen type III (rColMA) loaded with basic fibroblast growth factor (bFGF)-encapsulated liposomes (bFGF@Lip). This design enables the controlled release of bFGF, creating an optimal environment for the growth and differentiation of bone marrow mesenchymal stem cells (BMSCs) into cholangiocyte-like cells. These cells are instrumental in the regeneration of bile duct tissues, evidenced by the pronounced expression of cholangiocyte differentiation markers CK19 and CFTR. The PEGDA//rColMA/bFGF@Lip bilayer bile duct scaffold can well simulate the bile duct structure, and the outer rColMA/bFGF@Lip hydrogel can well promote the growth and differentiation of BMSCs into bile duct epithelial cells. In vivo experiments showed that the scaffold did not cause cholestasis in the body. This new in vitro pre-differentiated active 3D printed scaffold provides new ideas for the study of bile duct tissue replacement.
研究领域:
组织工程
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生物医学工程
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再生医学
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外科研究
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PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Aili Suo,Weijun Xu,Yaping Wang,Tiantian Sun,Lijie Ji,Junmin Qian
In tumor biology, it is widely recognized that 3D rather than 2D cell culture can recapitulate key features of solid tumors, including cell-extracellular matrix (ECM) interactions. In this study, to mimick the ECM of breast cancer, hyaluronic acid (HA) hydrogels were synthesized from two polyvalent HA derivatives through a hydrazone and photo dual crosslinking process. HA hydrogels could be formed within 120 s. The hydrogels had similar topography and mechanical properties to breast tumor and displayed glutathione and hyaluronidase dual-responsive degradation behavior. Biological studies demonstrated that HA hydrogel could support the proliferation and clustering of breast cancer MCF-7 cells. The expression levels of VEGF, IL-8 and bFGF in hydrogel-cultured cells were significantly greater than those in 2D culture. Moreover, cells from hydrogel culture exhibited greater migration/invasion abilities and tumorigenicity than 2D-cultured cells. Therefore, the HA hydrogels are a promising ECM-mimicking matrix for in vitro construction of breast cancer.
研究领域:
生物材料
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癌症生物学
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组织工程
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细胞生物学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2023
·
IF 7
Jiahui Zhou,Kaishun Xia,Yuting Li,Shihua Mao,Yucong Gu,Mengjie Si,Shuaibing Wang,Guangyan Du,Yisheng Xu,Dong Zhang,Si Yu Zheng,Jintao Yang
Efficient wound healing has attracted great interest due to the prevalence of skin damage. It is still highly desired yet challenging to construct a multi-drug loaded wound dressing that can release different drugs at different times to meet specific requirements towards different healing stages. Herein, a wound dressing was developed based on thermoresponsive zwitterionic nanocapsules (ZNs) that were sandwiched between two double-layered fabrics to regulate the multiple drug release pathway. The salt-response of the obtained ZNs was greatly suppressed while its transition temperature was regulated to be ∼37 °C to fit the needs of the physiological environment. Two bioactive substances, human basic fibroblast growth factor (bFGF) for tissue regeneration and norfloxacin for anti-inflammation, were loaded in the ZNs and on the surface of fabrics, respectively, to achieve separative gradient release. The in vitro drug release tests revealed that norfloxacin could be released relatively fast (∼24 h) while the release rate of bFGF was much slower (∼168 h), matching the specific time requirements of inflammation and proliferation stages very well. The in vivo wound healing experiment also confirmed the high wound healing efficiency of the wound dressing developed here, compared to the wound dressings without gradient release characteristics. We believe the strategy illustrated here will provide new insights into the design and biomedical applications of zwitterionic nanocapsules.
研究领域:
材料科学
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生物医学工程
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药学
·
组织工程
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DOI
PubMed
高影响因子
ACS Applied Bio Materials
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2020
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IF 0
Jinyu Hu,Yi Song,Cuiyun Zhang,Wen Huang,Anqi Chen,Huacheng He,Susu Zhang,Yanxin Chen,Chaodong Tu,Jianhui Liu,Xuan Xuan,Yung Chang,Jie Zheng,Jiang Wu
Development of biocompatible and bioactive drug-loaded sutures is considered as an effective but challenging strategy for the wound healing process by delivering biological drugs (e.g., antibiotics) or growth factors (e.g., bFGF) at the surgical wound sites. Conventional offline suture strategies often lead to fast and uncontrollable release of drugs at wound sites, rendering wound healing to become a longer and painful process for patients. Herein, we propose an online suture strategy to fabricate electrospun polycaprolactone (PCL) fibrous yarns, incorporated with both collagen (COL) and bFGF, to produce bFGF-COL@PCL sutures. Upon demonstrating the well-oriented and aligned fibrous microstructure, high mechanical properties, and controlled release of bFGF from bFGF-COL@PCL sutures in vitro, we then applied bFGF-COL@PCL sutures to an incision wound healing mouse model in vivo. Further in vivo study showed that as compared to the commercialized Vicryl suture, bFGF-COL@PCL sutures significantly promoted the wound healing at different stages by accelerating granulation tissue formation, collagen deposition, and re-epithelialization. The enhanced wound healing efficiency of bFGF-COL@PCL sutures is likely attributed to two synergistic factors: (i) the well-oriented nanofibrous structure reduces tissue drag to minimize their trauma and (ii) the presence of both collagen and bFGF enhances the basement membrane (BM) reconstruction, cell proliferation, and angiogenesis. This work demonstrates an effective suture strategy and system for surgical suture applications.
研究领域:
生物医学工程
·
组织工程
·
药剂学
·
材料科学
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产品关联
DOI
PubMed
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2024
·
IF 3.8
Yining Chen,Qinxin Jing,Ying Zhang,Xinghan Wu,Zhongxiang Bai,Nianhua Dan,Zhengjun Li,Yuwen Li,Yunbing Wang
Diabetic wound usually accompanied by adverse factors such as hyperglycemia, oxidative stress damage, and inflammatory response is a typical refractory chronic wound. An insufficient regulation of the wound microenvironment is the limiting factor of diabetic wound healing. A drug-loaded micelle is a potential material for the treatment of diabetic wounds. Herein, an injectable, self-healable, and multiresponsive hydrogel (hydrogel@MIC&bFGF) was developed for the integrated diabetic wound therapy by a facile double-injection strategy. Meanwhile, the anti-inflammatory acetyl-11-keto-β-boswellic acid (AKBA) from a medicinal plant loaded in the hyaluronic acid-based micelles (MIC@AKBA) was encapsulated in the hydrogel along with basic fibroblast growth factor (bFGF). Hydrogel@MIC&bFGF had integrated rheology properties, antioxidant properties, and multiresponse abilities that realized the sustained release of AKBA and bFGF to treat inflammation and promote wound healing in response to glucose, reactive oxygen species, and an acidic environment. In vitro and in vivo experiments substantiated that hydrogel@MIC&bFGF showed the desired therapeutic effects for the treatment of full-thickness skin defects in diabetic rats, which promoted wound healing by reducing inflammation and oxidative stress while accelerating angiogenesis. This study provides a potential strategy for drug-loaded hydrogels for diabetic wound treatment.
研究领域:
药剂学
·
材料科学
·
生物医学工程
·
伤口愈合
✓ 已验证
DOI
高影响因子
Scientific Reports
·
2015
·
IF 5.578
Shixuan Chen,Junbin Shi,Min Zhang,Yinghua Chen,Xueer Wang,Lei Zhang,Zhihui Tian,Yuan Yan,Qinglin Li,Wen Zhong,Malcolm Xing,Lu Zhang,Lin Zhang
The purpose of this study was to permit bone marrow mesenchymal stem cells (BMSCs) to reach their full potential in the treatment of chronic wounds. A biocompatible multifunctional crosslinker based temperature sensitive hydrogel was developed to deliver BMSCs, which improve the chronic inflammation microenvironments of wounds. A detailed in vitro investigation found that the hydrogel is suitable for BMSC encapsulation and can promote BMSC secretion of TGF-β1 and bFGF. In vivo, full-thickness skin defects were made on the backs of db/db mice to mimic diabetic ulcers. It was revealed that the hydrogel can inhibit pro-inflammatory M1 macrophage expression. After hydrogel association with BMSCs treated the wound, significantly greater wound contraction was observed in the hydrogel + BMSCs group. Histology and immunohistochemistry results confirmed that this treatment contributed to the rapid healing of diabetic skin wounds by promoting granulation tissue formation, angiogenesis, extracellular matrix secretion, wound contraction, and re-epithelialization. These results show that a hydrogel laden with BMSCs may be a promising therapeutic strategy for the management of diabetic ulcers.
研究领域:
干细胞生物学
·
伤口愈合
·
糖尿病
·
药剂学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）