ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Yingxin Chen,Qianying Sheng,Yayun Hong,Minbo Lan
A hydrophilic nanocomposite was synthesized by an easy route to improve glycopeptides enrichment efficiency. This new composite, prepared with a method based on electrostatic interaction, was demonstrated to be efficient for immobilization of carrageenan on graphene oxide/poly(ethylenimine) support (denoted as GO–PEI–Carr). Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics. The introduction of carrageenan provided the composite not only a perfect surface charge but also a greater ability to enrich glycosylated peptides. Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio. A good selectivity was still kept even when the molar ratio of IgG and bovine serum albumin (BSA) tryptic digest mixtures reached to 1:500. Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples. In addition, GO–PEI–Carr exhibited a unique repeatability and stability even after enrichment of glycopeptides for 20 times. It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.
研究领域:
材料科学
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分析化学
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生物化学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2018
·
IF 5.089
Jingrong Liu,Hualin Wang,Pengfei Wang,Min Guo,Suwei Jiang,Xingjiang Li,Shaotong Jiang
Novel films based on κ-carrageenan (Car) incorporated with curcumin (Cur) were developed for freshness monitoring of foods . Cur and Car were interacted by hydrogen bonds. A low amount of Cur (no higher than 3%) could be well-dispersed in Car matrix. These characteristics significantly improved the film barrier and tensile strength, besides the enhancement of thermal stability. Hence, the Car film with 3% Cur exhibited the best performance. The films showed a good release control of Cur and a predominant Fickian diffusion release mechanism, which was favorable to sustainable pH change sensing. The Car-Cur films demonstrated a strong redness-shift under alkaline condition. This characteristic was successfully used to monitor the spoilage of pork and shrimp during storage. As a pH colorimetric indicator, the Cur incorporated Car films may have a great potential in the freshness monitoring of foods.
研究领域:
食品科学
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材料科学
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生物学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Nan Wang,Jie Tian,Linlin Wang,Shuang Song,Chunqing Ai,Srinivas Janaswamy,Chengrong Wen
Fucoidan (FUC) is a non-gelling polysaccharide but could interact with κ-carrageenan (KC) to form a stable gel blend. However, their interaction mechanism is unclear. Herein, FUC and KC blended gels are prepared by mixing FUC (10 and 20 mg/mL) and KC (6, 7 and 8 mg/mL) solutions, and characterized through LF-NMR, rheology , DSC , Cryo-SEM, and FTIR. The FTIR analysis confirms the formation of hydrogen bonds between FUC and KC chains. The KC addition to FUC significantly improves the water retention and frost resistance. The viscoelastic measurements reveal higher gelling nature of the FUC-KC binary mixtures, and the DSC results confirm the higher thermal stability . The Cryo-SEM images clearly reveal the gel network structure. The outcome of this study deemed to further the FUC use in food and non-food applications.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2020
·Vol. 109
·期 6
·829-839
·
IF 3.525
Yongrui Huang,Lejiao Zhang,Rijian Song,Xuan Mao,Shunqing Tang
A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation. Release behavior demonstrated that the hybrid carrageenan contained sponge was mechanically stable and could release carrageenan constantly. RT-PCR and ELISA experiments showed that the leaching liquor of the sponge could stimulate RAW264.7 from M0 state to a polarized state by secreting more anti-inflammatory factor IL-10 than pro-inflammatory ones, such as, IL-6 and TNF-α. Transwell experiments also indicated that the leaching liquor could promote the proliferation of NIH-3T3 by stimulating RAW264.7 of M0 state after 7 days. Results of particle size and intracellular concentration analyses suggested that the released carrageenan might enter into the cellular interior of RAW264.7 in the form of microgels or protein complexes. The sponge would be a promising candidate for skin wound dressing.
研究领域:
生物材料
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免疫学
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组织工程
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 3
·1558-1566
·
IF 3.9
Xueqi Gan,Chen Li,Jiyu Sun,Xidan Zhang,Min Zhou,Yi Deng,Anqi Xiao
Hydrogels are crosslinked hydrophilic polymer networks of high-water content. Although they have been widely investigated, preparing hydrogels with excellent mechanical properties and biocompatibility remains a challenge. In the present work, we developed a novel GelMA/κ-carrageenan (GelMA/KC) double network (DN) hydrogel through a dual crosslinking strategy. The three-dimensional (3D) microstructure of KC is the first network, and covalently crosslinked on the κ-carrageenan backbone is the second network. The GelMA/KC hydrogel shows advantages in physical properties, including higher compression strength (10% GelMA/1% KC group, 130 kPa) and Young's modulus (10% GelMA/1% KC group, 300), suggesting its excellent elasticity and compressive capability. When using a higher concentration of GelMA, the hybrid hydrogel has even higher mechanical properties. In addition, the GelMA/KC hydrogel is favorable for cell spreading and proliferation, demonstrating its excellent biocompatibility. This study provides a new possibility for a biodegradable and high-strength hydrogel as a new generation material of orthopedic implants.
研究领域:
材料科学
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生物医学工程
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组织工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMERS AND THE ENVIRONMENT
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2023
·Vol. 31
·期 6
·2653-2667
·
IF 5.3
Naureen Bushra,Ang Bee Chin,Muhamad Farina,Haseeb A. S. M. A.,Basirun W. J.
Polyurethane (PU) are versatile synthetic polymers used for scaffold synthesis. However, their biocompatibility and growth factor (GF) release properties for biomedical and tissue engineering (TE) applications need improvement. We report four kappa-carrageenan (κ-CRG) modified PU (CMPU: PU4C1, PU4C2, PU4C3, and PU4C4) scaffolds from PU4 where κ-CRG % is minimum in PU4C1 and it is maximum in PU4C4. The investigations include fourier transformed infrared (FTIR) spectroscopy, energy-dispersive X-ray (EDX) spectroscopy, in-vitro cell-viability assays using C2C12 myoblasts, and GF such as insulin-like growth factor-1 (IGF-1) release studies using enzyme linked immuno sorbent assay (ELISA) kits. FTIR spectra confirmed the synthesis of CMPU (C = C, COOR, NHCOO, O = S = O, C–O–C, O–SO 3 − ) scaffolds. The EDX spectra presented an increase of sulfur (%) from 0.24% (PU4C1) to 4.0% (PU4C4) with the increase of κ-CRG. The results of biocompatibility assessment day-21 (%) cell viability (PU4 = 67.54 ± 7.68%, PU4C1 = 71.40 ± 5.07%, PU4C2 = 75.79 ± 6.10%, PU4C3 = 82.42 ± 6.82%, PU4C4 = 83.37 ± 7.68%), live/dead images and scanning electron microscope (SEM) images (day 1, 7, and 14) suggest that C2C12 myoblasts adhesion, proliferation, metabolic activity, and viability followed a trend (PU4C4 > PU4C3 > PU4C2 > PU4C1 > PU4). A similar trend was observed in the IGF-1 (ng ml −1 ) release from PU4 and CMPUs (day 1, 3, 7, 10, and 14), suggesting a successful synthesis of CMPUs with enhanced biocompatibility and GF (IGF-1) release, i.e., directly related to the incorporated κ-CRG concentration. Graphical Abstract
研究领域:
生物材料
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组织工程
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生物医学工程
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材料科学
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产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Cailing Tong,Chen Huang,Haoyu Lai,Kailin Li,Xinxin Zeng,Chengrong Wen,Chunhua Wu,Jie Pang
Konjac glucomannan/κ-carrageenan (KGM/KC) composite hydrogels often fail to meet industrial requirements due to their weak mechanical properties, while nanofillers are potential materials to address this challenge by taking advantage of their nano-size and filling effects. Herein, carboxylated cellulose nanocrystals (C–CNCs) were chosen as nanofillers to regulate the properties of KGM/KC composite hydrogels, with focus on the effect of C–CNCs concentration on the morphological and physicochemical properties. Meanwhile, an in-depth exploration of the possible mechanisms was also underlying. C–CNCs were well dispersed within the KGM/KC composite matrix, which positively enhanced the mechanical strength, as well as increased the apparent viscosity and modulus of the composite hydrogel greatly when the C–CNCs concentration was 10 % (w/w). This is mainly ascribed to the enhancement of hydrogen bond interaction among the KGM, KC, and C–CNCs, which were verified by Fourier transform infrared spectroscopy. Additionally, the results of low-field nuclear magnetic resonance confirmed the presence of C–CNCs in the hydrogel matrix reduced the water mobility of the hydrogel, which improved the freeze-thaw stability of the composite hydrogel. The fitting of the Power law model (R 2 > 0.98) showed that the swelling mechanism of KGM/KC composite hydrogels belonged to Fickian diffusion, and the swelling behavior could be regulated by adjusting the C–CNCs concentration. However, the excessive C–CNCs might weakened the interaction of composite hydrogels, which partially explains the reversal of the reinforcement effects seen at the lower additions of C–CNCs inclusion. These results provide a basis for the development and design of KGM/KC-based products with desirable attributes.
研究领域:
食品科学
·
材料科学
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DOI
高影响因子
Biofabrication
·
2024
·
IF 8.2
Hua Zhang,Yang Luo,Zeming Hu,Mengxi Chen,Shang Chen,Yudong Yao,Jie Yao,Xiaoqi Shao,Kerong Wu,Yabin Zhu,Jun Fu
Three-dimensional (3D) bioprinting embedded within a microgel bath has emerged as a promising strategy for creating intricate biomimetic scaffolds. However, it remains a great challenge to construct tissue-scale structures with high resolution by using embedded 3D bioprinting due to the large particle size and polydispersity of the microgel medium, as well as its limited cytocompatibility. To address these issues, novel uniform sub-microgels of cell-friendly cationic-crosslinked kappa-carrageenan (κ-Car) are developed through an easy-to-operate mechanical grinding strategy. These κ-Car sub-microgels maintain a uniform submicron size of around 642 nm and display a rapid jamming-unjamming transition within 5s, along with excellent shear-thinning and self-healing properties, which are critical for the high resolution and fidelity in the construction of tissue architecture via embedded 3D bioprinting. Utilizing this new sub-microgel medium, various intricate 3D tissue and organ structures, including the heart, lungs, trachea, branched vasculature, kidney, auricle, nose, and liver, are successfully fabricated with delicate fine structures and high shape fidelity. Moreover, the bone marrow mesenchymal stem cells encapsulated within the printed constructs exhibit remarkable viability exceeding 92.1% and robust growth. This κ-Car sub-microgel medium offers an innovative avenue for achieving high-quality embedded bioprinting, facilitating the fabrication of functional biological constructs with biomimetic structural organizations.
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yalei Liu,Yuying Zhao,Sui Wang,Jie Mao
The development of green packaging is of great significance in response to the increasing demand for environmental sustainability. In this study, a composite packaging film was prepared using carrageenan and polyvinyl alcohol as the base materials, with cinnamaldehyde incorporated as a natural active ingredient. The cinnamaldehyde was successfully integrated into the carrageenan/polyvinyl alcohol composite network, endowing the film with antibacterial properties. Results indicate that the composite film possesses satisfactory mechanical properties, excellent UV-blocking performance (UV transmittance ≤37.6 %), high transparency (>80 %), and outstanding oxygen barrier properties (exhibiting an oxygen transmission rate as low as 1.696 cm 3 /m 2 ·24h·0.1 MPa), meeting the requirements for fundamental application scenarios. Furthermore, the water solubility of the film allows it to be decomposed and disposed of via simple water treatment after use. This characteristic offers a promising strategy for developing green alternatives to traditional petroleum-based packaging materials, highlighting its potential for reducing white pollution and promoting the sustainable development of the packaging industry.
研究领域:
材料科学
·
食品包装
·
高分子复合材料
·
可持续材料
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Jianfu Qiao,Biyang Ling,Xiangyi Han,Hong Gu,Qi Wang
Full-thickness skin defects present substantial clinical challenges owing to the complete disruption of normal tissue architecture, elevated susceptibility to microbial infection, and the inherently limited regenerative capacity of damaged skin. Accordingly, the development of injectable biomaterials that integrate antibacterial activity with regenerative functionality is essential for facilitating efficient skin repair. This study presents an environmentally friendly strategy for the construction of a silver nanoparticle-incorporated carrageenan–hyaluronic acid composite antibacterial hydrogel intended for full-thickness skin wound repair. Under visible light, silver ions simultaneously participate in the cross-linking reaction of carrageenan and are reduced to uniformly dispersed silver nanoparticles, thereby endowing the hydrogel with a stable structure and antibacterial activity without the need for exogenous cross-linking agents and chemical reducing agents. The resulting hydrogel exhibited good injectability, high water content, biocompatibility, and biodegradability, and had a broad-spectrum antibacterial effect against both Gram-positive and Gram-negative bacteria. In vitro experiments demonstrated that the hydrogel exhibited excellent cytocompatibility and significantly enhanced cellular activity. Moreover, evaluation in animal models of full-thickness skin defects confirmed that the injectable carrageenan–hyaluronic acid–silver nanohydrogel effectively accelerated wound closure within 14 days. This environmentally friendly, antibacterial polysaccharide-based hydrogel offers a novel strategy for the design of injectable wound dressings and shows promising application prospects in skin wound repair and regenerative medicine.
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2026
·
IF 3.9
Jiang Li,Zhiyan Wang,Xiaoqian Gu,JiaoJiao Tan,Luying Zhao,Long Chen,Xiangyu Cong,Xinning Pan
The biological activities of marine algal oligosaccharides (MAOs) have garnered widespread attention today. Compared with κ-carrageenase and its enzymatic hydrolysates, the research on ι-carrageenase and its enzymatic hydrolysates is still in its infancy. In this study, the ι-carrageenan oligosaccharide, ι-COs-L (DP2, DP4) and ι-COs-H (DP8) prepared by ι-carrageenase Car1293, dose-dependently suppressed the overexpression of TNF-α, IL-6, IL-1β, and NO while improve the expression of TGF-β1 and IL-10 in lipopolysaccharide-induced RAW264.7 inflammation model and exerted excellent anti-inflammatory effect via NF-κB/IκBα signaling pathway Moreover, different concentrations of ι-COs-H effectively alleviated DSS-induced colitis symptoms in mice by modulating gut microbiota composition, promoting short-chain fatty acid (SCFA) production, and enhancing intestinal barrier function. Transcriptomic and Western blot analyses indicated that the gut microbiota-SCFA-PPARγ axis plays an important role in the maintenance of intestinal homeostasis, which may represent a potential approach for colitis prevention by ι-carrageenan oligosaccharide.
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DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2017
·
IF 5.951
Ning Ma,Xuehua Liu,Ziqi Yang,Guojun Tai,Yanru Yin,Shuibo Liu,Hongliang Li,Peizhi Guo,X. S. Zhao
Palladium (Pd) nanoflowers with tunable thorns were prepared by a facile and rapid route with the assistance of carrageenan. The superior nature of well-dispersed Pd nanoflowers has been disclosed by X-ray diffraction, scanning electron microscopy, and transmission electron microscopy measurements. The growth of thorns of Pd nanostructures was first stimulated with the continuous introduction of l-ascorbic acid in the synthesis system and then reached a maximum length of about 132 nm, followed by a slight decrease in concentration. Electrochemical data showed that Pd nanoflowers with the longest thorns exhibited the highest catalytic current density of 1160 mA/mg, optimized tolerance to the poisoning, and the lowest onset potential while others showed a better cycle stability with a catalytic activity maintenance of above 96% after 300 cycles. It is suggested that the structure of thin thorns of Pd nanoflowers should be easier to damage than thick thorns during the electrocatalysis of ethanol. The relationship between Pd nanoflowers and properties as well as the key factors to form the nanoflower structure were discussed based on the experimental data.
研究领域:
材料科学
·
电化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）