INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.7
Xiaohui Wang,Lu Wang,Qihao Ma,Shihao Dai,Wenyan Li,Jiaju Li,Guangyu Xu,Bin Liang,Chanchan Sun
Three nonionic surfactants increased the yield of Monascus EPS by 10% to 20%. • The addition of nonionic surfactants enhances the permeability of the cell membrane. • The addition of nonionic surfactants inhibited the biosynthesis of citrinin. • T-EPS exhibits the strongest enzyme inhibitory ability and glucose adsorption capacity. • The high hypoglycemic activity of T-EPS is closely related to its physicochemical structure. Monascus extracellular polysaccharides exhibit diverse bioactivities, including antioxidant, antitumor, and immunomodulatory effects. Nevertheless, their low native yield restricts practical applications. To improve the fermentation yield and functional properties of EPS, Monascus purpureus YY-1 was employed as the experimental strain in this study. We systematically investigated the regulatory effects of three nonionic surfactants (Triton X-100, Brij 35, and Span 60) on EPS production, citrinin accumulation, physicochemical structures of polysaccharides, and their in vitro hypoglycemic activity of the strain. The results demonstrated that nonionic surfactants significantly elevated EPS yields by enhancing fungal cell membrane permeability, achieving an overall increase of 10%–20% relative to the blank control group. Meanwhile, these surfactants effectively inhibited the biosynthesis and accumulation of citrinin during fermentation, improving product safety. Among these, the polysaccharides (T-EPS) obtained with Triton X-100 exhibit higher total sugar content, a more favorable galactose-to-mannose ratio, lower average molecular weight and particle size, and display a typical triple-helix conformation and a loose, porous micro-morphology. These favorable physicochemical structural features confer T-EPS with stronger inhibitory activities against α-amylase and α-glucosidase, as well as superior glucose adsorption capacity. This study confirms that nonionic surfactants (especially Triton X-100 with optimal performance) can synergistically enhance the yield, safety, and hypoglycemic activity of Monascus EPS. It provides important theoretical support for the development and application of functional polysaccharides from Monascus in the field of hypoglycemic functional foods.
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DOI
高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
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2020
·Vol. 129
·期 5
·1410-1418
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IF 3.066
Li Z.,Liu Y.,Li Y.,Lin F.,Wu L.
AimsRed yeast rice (RYR), produced by inoculating Monascus strains to steamed rice, contains many kinds of physiologically bioactive compounds, among which monacolin K can be used as an antihypercholesterolaemic agent. However, RYR can be polluted by the mycotoxin citrinin, which has nephrotoxic and hepatotoxic activities. To avoid the risk of citrinin contamination in Monascus fermented products, it is important to screen for Monascus strains that produce no or low citrinin.Methods and ResultsFive autochthonous Monascus strains with high‐yield monacolin K and undetectable citrinin were obtained using high‐performance liquid chromatography (HPLC). All five strains were identified as Monascus ruber based on Genealogical Concordance Phylogenetic Species Recognition criteria. Polymerase chain reaction revealed that citrinin polyketide synthase (pksCT) gene was found in these strains, but transcriptional regulator (ctnA) was not found.ConclusionsFive strains are potential strains for producing high‐quality RYR. The distribution of the pksCT gene was not restricted to Monascus purpureus and Monascus sanguineus, and M. ruber strains were diverse in pksCT and ctnA genes.Significance and Impact of the StudyThe integration of citrinin HPLC analysis and pksCT and ctnA genes amplification could provide a complementary approach in valuable Monascus strains screening.
研究领域:
微生物学
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食品科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
BIOMEDICAL CHROMATOGRAPHY
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2018
·Vol. 32
·期 8
·e4237
·
IF 1.688
Yanqing Liu,Hongwu Wang,Su Yao,Peijie Zhu
A fast and sensitive method involving ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) was introduced to detect citrinin in dried orange peel. A series of extraction, purification and chromatographic conditions was also systematically examined. With the proposed method, the obtained calibration graph was linear, with an R of 0.9996 within a concentration range of 0.5–10 ng/mL. The estimated limits of detection and quantification were 0.05 and 0.17 ng/mL, respectively. Under the selected conditions, the relative recoveries in different citrus products spiked with 1–10 ng/mL citrinin were 89.4–98.7% with RSDs of <2.5%. Compared with previously reported analytical methods, the newly developed UPLC–MS/MS method showed excellent sensitivity and good precision in detecting citrinin. The results indicated that it is a reliable and effective technique for the detection of trace citrinin in dried orange peel.
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2021
·
IF 7.514
Xiaopeng Hu,Yiwei Liu,Yide Xia,Faqiong Zhao,Baizhao Zeng
Citrinin can cause serious human diseases, thus its detection in foods is necessary. Herein, a molecularly imprinted polymer-based ratiometric electrochemical sensor (MIP-RECS) was presented for citrinin detection. The sensor was fabricated by electropolymerization, using thionine as monomer and citrinin as template. The ionic liquid decorated boron and nitrogen co-doped hierarchical porous carbon (BN-HPC) as supporter, provided large surface for anchoring thionine and citrinin. Poly(thionine) not only acted as MIP, but also acted as reference probe. When [Fe(CN) 6 ] 3-/4- was adopted as indicating probe, the resulting sensor demonstrated a wide linear detection range (i.e. 1 × 10 -3 –10 ng mL −1 ) and a low detection limit (i.e. 1 × 10 -4 ng mL −1 ).The sensor was applied to the detection of spiked citrinin in real samples, and satisfactory recovery (i.e. 97% – 110%) was obtained. Hence, it was promising for citrinin detection.
研究领域:
食品科学
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分析化学
·
毒理学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）