PeerJ
·
2021
·
IF 2.984
Yufan Zhu,Zhiqiang Yang,Yuanlong Xie,Min Yang,Yufeng Zhang,Zhouming Deng,Lin Cai
Objective This study aims to explore the effect of daidzein, which is a natural isoflavone compound mainly extracted from soybeans, on osteosarcoma and the potential molecular mechanism. Material and Methods 143B and U2OS osteosarcoma cells were treated with gradient concentrations of daidzein, and MTT assay was used to determine the cell proliferation capacity and IC 50 . Hoechst 33342 staining and Annexin V-FITC/PI detection were used to determine apoptosis. Cell cycle was analyzed by flow cytometry, and migration ability were detected by transwell assays and scratch wound assay. An osteosarcoma xenograft mice model was applied to investigate the effect of daidzein on osteosarcoma in vivo . Systematic pharmacology and molecular modeling analysis were applied to predict the target of daidzein to osteosarcoma, and the target Src was verified by western blotting. We also observed the effect of daidzein on cell proliferation and apoptosis of Src-overexpressing osteosarcoma cells. Results In vitro , daidzein significantly inhibited 143B and U2OS osteosarcoma cell proliferation and migration, and induced cell cycle arrest. In vivo , daidzein exerts antitumor effects in osteosarcoma xenograft mice. After systematic screening and analysis, Src-MAPK signaling pathway was predicted as the highest-ranked pathway. Western blot demonstrated that daidzein inhibited phosphorylation of the Src-ERK pathway in osteosarcoma cells. Also, overexpression of Src could partially reverse the inhibitory effects of daidzein on osteosarcoma cell proliferation. Conclusion Daidzein exerts an antitumor effect on osteosarcoma, and the mechanism may be through the Src-ERK pathway.
研究领域:
肿瘤学
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药理学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2017
·
IF 4.529
Shuya Wang,Ziwei Yang,Na Peng,Jun Zhou,Xiaoyu Yong,Haoran Yuan,Tao Zheng
Ionic liquids-based microwave-assisted hydrolysis was successfully applied for hydrolysis of puerarin and daidzein derivatives from Radix Puerariae Lobatae extract. The ionic liquids with different cations and anions have been investigated, and [Omim]HSO 4 was selected as catalyst. In addition, the hydrolysis parameters, including catalyst quantity, ionic liquids concentration, microwave power and microwave time, were optimized by Response Surface Methodology. The optimal conditions were as follows: 0.82 mol/L [Omim]HSO 4 ; catalyst quantity, 1.42 mL; microwave time, 7 min; microwave power, 400 W. Under those condition the average hydrolysis efficiency of puerarin and daidzein derivatives was 57.93 ± 3.08%. Compared with the traditional acidic catalysts, the [Omim]HSO 4 exhibited higher efficiency, which demonstrates that ionic liquids-based microwave-assisted hydrolysis was a rapid, efficient, and simple hydrolysis technique.
研究领域:
中药化学
·
分析化学
✓ 已验证
DOI
高影响因子
Food & Function
·
2026
·
IF 6.3
Ziyuan Zhou,Xiaxia Pan,Jiali Ni,Zhuoqi Lou,Yangfan Shen,Shiman Jiang,Xiangmin Dong,Fengjiao Wang,Yanfei Chen,Lanjuan Li
Existing evidence indicates that pre-existing metabolic dysfunction-associated steatotic liver disease (MASLD) confers susceptibility to acute liver injury (ALI). Nevertheless, the underlying mechanism prompting ALI risk in such condition is not fully uncovered yet. Gut dysbiosis has been well-documented in MASLD patients which prompts us to hypothesize that gut dysbiosis may serve as a contributing factor and regulating target to ALI susceptibility. A case-control study was performed and verified the increased risk of ALI in MASLD patients. Mice pretreated with MASLD mice/patients-derived fecal microbiota transplantation (FMT), demonstrated worsened ALI after D-GalN/LPS injection. We identified significant gut dysbiosis with decreased microbial α-diversity, beneficial genus lactobacillus, increased potential harmful bacteria Campylobacter and helicobacter. Probiotic Akkermansia muciniphila (A. muciniphila) was reported to improve gut dysbiosis. Thus, we tested the effect of A. muciniphila on ALI mice pretreated with MASLD mice-derived FMT. A. muciniphila significantly ameliorated the worsened ALI and improved gut dysbiosis. Transcriptomic analysis reveled that A. muciniphila restored the impaired liver PPAR-α signaling, alleviating the aggravated NF-κB signaling and downstream inflammation response in liver. Specifically, A. muciniphila restored the decreased abundance of PPAR-α agonist daidzein in colon, thereby restoring PPAR-α activation and inhibiting NF-κB signaling in liver. Daidzein is also capable to ameliorate ALI aggravated by MASLD-associated dysbiosis, accompanied by PPAR-α activation and NF-κB inhibition. Our findings revealed that gut dysbiosis in MASLD increased the risk of ALI. A. muciniphila exhibited preventive potential for MASLD patients to reduce ALI risk. Daidzein/PPAR-α/NF-κB signaling is important to maintain host resistance to hepatoxic challenge.
✓ 已验证
DOI
PubMed
高影响因子
POLYMER
·
2025
·
IF 4.5
Pan Tian,Weiyouran Hong,Yisen Huang,Wanjing Liu,Shengdu Yang,Xin Sun,Junhua Zhang
The development of biobased renewable resources is expected to alleviate the threat to the ecological environment and the obstacles to sustainable development caused by relying on fossil fuels. Here, based on biobased daidzein (DD) with rigid benzene rings and two hydroxyl groups was firstly prepared into epoxy compound (DDDE) by reacting with epichlorohydrin. Daidzein is a liquid crystal mesogen, by curing with 1,5-naphthalene diamine (NDA) in the liquid crystal state, the locally ordered structure can be preserved, thereby exhibiting intrinsic thermal conductivity characteristics. The curing system DDDE/NDA 105 (105 means cured at 105 °C) under liquid crystal phase obtained better thermal conductivity (λ = 0.34 W/(m·K)), storage modulus (G’ = 2548 MPa), and glass transition temperature (T g = 230 °C) than E51/NDA. On the other hand, photocurable alkali developer resin (DDAT) was synthesized by DDDE with acrylic acid (AA) and tetrahydro phthalic anhydride (THPA), and subsequently, a series of DDAT/DDDE/filler composite inks were prepared. Finally, the C8:2 ink stands out on PCB with the comprehensive performance of tensile strength (53.67 ± 1.41 MPa), elongation of fracture (4.11 ± 0.23 %), G’ (3761 MPa), T g (227 °C), and dielectric constant (3.54 at 100 Hz). The present work broadens the application of bio-based daidzein in high-performance inherently thermally conductive epoxy resins and alkaline developing photocuring inks.
研究领域:
高分子材料科学
·
生物基材料
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热功能材料
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光响应材料
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介电材料
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绿色化学
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先进涂层技术
✓ 已验证
产品关联
DOI
高影响因子
Polymers
·
2025
·Vol. 17
·期 1
·94
·
IF 4.9
Yufei Jia,Yimiao Zhang,Fuliang Meng,Zeyu Chen,Hongwei Fei,Dapeng Zhou,Maiyong Zhu,Xinhua Yuan
The preparation of biological phenolic resin (PF) with green recyclable biomaterials instead of phenol is a research hotspot for solving current resource and environmental problems. In this study, on the basis of introducing lignin into the phenolic system, daidzein of a renewable resource with a rigid structure was selected to modify lignin-based phenolic resin (LPF), and the improvement of the mechanical and thermal properties of the modified phenolic resin under different substitution ratios was studied. The friction materials were prepared with a daidzein-modified lignin-based phenolic resin (D-LPF) as the matrix binder, and their effects on the mechanics and friction and wear properties of friction materials were investigated. The results show that when the proportion of daidzein replacing phenol is 12%, the highestTpcan reach 152.4 °C, and theTgof the modified D-LPF resins is significantly higher than those of PF and LPF. The highestTsof D-LPF is 203.3 °C, which is also significantly higher than those of PF and LPF (184.7 °C and 174.6 °C, respectively). The maximum carbon residue rate at 800 °C is 64.2% and is greatly improved compared with the 55.1% and 56.7% of PF and LPF. The bending strength and impact strength of D-LPF-matrix friction materials are obviously higher than those of PF- and LPF-matrix friction materials. The specific wear rate of D-LPF-matrix friction materials is 0.70 × 10−4mm3/Nm, which is obviously lower than those of PF- and LPF-matrix friction materials and shows good applicational prospect as a matrix resin in friction materials.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2024
·
IF 4.4
Guangjian Dai,Shengtao Zhang,Wenjie Yang,Chunxiang Wei,SanE Zhu,Zhongxing Geng,Eric W. M. Lee,Hongdian Lu,Bin Yu,Wei Yang
The exploration of biomass-derived thermosetting resins for implementation in flexible electronic packaging has emerged as a central focus. Herein, a daidzein-derived monomer (DDAB) was synthesized, which featured carbon–carbon double bonds. Biobased resins, namely, DDAB/3SH and DDAB/4SH, were meticulously fabricated via photocuring process, entailing the amalgamation of DDAB with trithiol (TPTMP) and tetrathiol (PETMP) monomers, respectively. DDAB/4SH showed impressive mechanical properties with a tensile strength of 28.1 MPa and elongation at break of 21.3%. Notably, owing to the augmented free volume inherent within the system, DDAB/3SH exhibited an exceptional elongation at a break of 111.7%, thereby surpassing the performance metrics of the majority of previously documented thiol-containing compounds. Moreover, both DDAB/3SH and DDAB/4SH demonstrated desirable dielectric properties. Specifically, within the frequency range of 15–16 GHz, DDAB/3SH achieved a minimum dielectric constant of 2.58. The exceptional dielectric performance, coupled with the tunable mechanical properties, proves the substantial application potential of DDAB/SH biomass-derived resins for flexible electronic packaging applications.
研究领域:
材料科学
·
电子工程
·
生物质化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Yingchao Li,Xiaoyu Liu,Farong Lu,Huichao Li,Jiaming Zhang,Yawei Zhang,Wenchao Li,Weiping Wang,Miaomiao Yang,Zhining Ma,Hui Zhang,Xiaomian Zhou,Youjun Xu,Zhonggui He,Jin Sun,Tianhong Zhang,Qikun Jiang
Daidzein (DAN) is an isoflavone, and it is often found in its natural form in soybean and food supplements. DAN has poor bioavailability owing to its extremely low water solubility and first-pass metabolism. Herein, we hypothesized that a bioactivatable natural amino acid-bearing carbamate prodrug strategy could increase the water solubility and metabolic stability of DAN. To test our hypothesis, nine amino acid prodrugs of DAN were designed and synthesized. Compared with DAN, the optimal prodrug (daidzein-4′-O-CO-N-isoleucine, D-4′-I) demonstrated enhanced water solubility and improved phase II metabolic stability and activation to DAN in plasma. In addition, unlike the passive transport of DAN, D-4′-I maintained high permeability via organic anion-transporting polypeptide 2B1 (OATP2B1)-mediated transport. Importantly, D-4′-I increased the oral bioavailability by 15.5-fold, reduced the gender difference, and extended the linear absorption capacity in the pharmacokinetics of DAN in rats. Furthermore, D-4′-I exhibited dose-dependent protection against liver injury. Thus, the natural amino acid-bearing carbamate prodrug strategy shows potential in increasing water solubility and improving phase II metabolic stability to enhance the oral bioavailability of DAN.
研究领域:
药理学
·
药物化学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POULTRY SCIENCE
·
2025
·
IF 4.2
Yanling Sun,Tiantian Wang,Yu Zhang,Ning Ma,Xinghua Zhao,Xin He
Fatty liver hemorrhagic syndrome (FLHS) severely endangers the health and productivity of laying hens, marked by hepatic steatosis and impaired lipid metabolism. Daidzein-betaine cocrystal improves lipid metabolism in the diet-induced obesity mice. We investigated the effects of daidzein-betaine cocrystal diet on production performance, egg quality, intestinal injury, cecal microbiota, the metabolites of intestinal microbiota and hepatic lipid metabolism in FLHS laying hens. A total of 108 twenty-eight-week-old laying hens were allocated to 6 treatments: the control group (normal diet), model group (high-energy and low-protein (HELP) diet), daidzein group (200 mg/kg daidzein + HELP diet), betaine group (184.62 mg/kg betaine + HELP diet), physical mixture group (384.62 mg/kg physical mixture + HELP diet) and daidzein-betaine cocrystal group (384.62 mg/kg daidzein-betaine cocrystal + HELP diet). The findings showed that intake of daidzein, betaine, physical mixture and daidzein-betaine cocrystal effectively alleviated the disordered lipid metabolism in laying hens fed a HELP diet. Moreover, daidzein-betaine cocrystal was best supplement in the improvement of the lipid metabolism. The daidzein-betaine cocrystal modulated the dysbiosis induced by HELP-diet through reducing the abundance of Desulfovibrio and increasing the abundance of Rikenellaceae_RC9_gut_group and Unclassified_f_Lachnospiraceae , whereas enhancing β-diversity of the gut microbiome, to restore equilibrium. Fecal metabolomics analysis showed that the indicators related to the 6 differentially abundant metabolites (DAMs) of lipid metabolism were improved. Transcriptome analysis identified 12 differentially expressed genes (DEGs) related to lipid metabolism. DAMs and DEGs were mainly co-enriched in peroxisome proliferators-activated receptor (PPAR) signaling pathways. To sum up, our results showed that dietary daidzein-betaine cocrystal could alleviate hepatic metabolic disorders associated with FLHS in laying hens by regulating the "gut-liver axis". This highlights the significant potential of the daidzein-betaine cocrystal as a therapeutic agent for counteracting the impacts of a HELP diet.
研究领域:
动物科学
·
家禽营养
·
肠道微生物研究
·
代谢紊乱
·
植物药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2016
·
IF 1.78
Yinghui Deng,Yanhua Pang,Yafei Guo,Yufeng Ren,Fen Wang,Xiali Liao,Bo Yang
Daidzein is an isoflavone of naturally abundance existing in plants and foods which has attracted much attention for its significant benefits on human health. However, its application was severely limited by its poor solubilities, instability and low bioavailability. To overcome these drawbacks, inclusion complexes of daidzein with two cyclodextrin (CD) derivatives, i.e., 2-hydropropyl-β-cyclodextrin (HP-β-CD) and sulfobutyl ether-β-cyclodextrin (SBE-β-CD) were prepared and characterized both in solution and solid state by 1D and 2D NMR , XRD, SEM and elemental analyses. Fluorescence spectroscopy and the Job plot were used to demonstrate a mainly 1:1 inclusion mode between daidzein and CDs. Their thermal stabilities were evaluated with TG and DSC experiments. Moreover, water solubility of daidzein was significantly improved by inclusion complexation with CDs. These results might suggest valuable approaches to developments of new pharmaceutical formulations of daidzein.
研究领域:
药学
·
化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）