Microbial Cell Factories
·
2026
·
IF 5.8
Chen Lei,Zheng Zhijie,Chen Feifei,Wang Weipeng,Huo Siqi,Li Xiangyu,Zhang Alei,Chen Kequan
Background High-quality glycine is widely used in the cosmetics and pharmaceutical industries. Conventional chemical synthesis of glycine is disfavored because it relies on toxic reagents and complex downstream purification. Results Herein, we established an efficient two-step biosynthetic process for producing glycine from glucose. First, an l -threonine−overproducing strain was used to synthesis l -threonine via 50 L fed-batch fermentation, achieving a titer of 119.3 g/L. Then, a highly active l -threonine aldolase ( Pp LTA) was screened to convert l -threonine into glycine. To overcome thermodynamic and acetaldehyde inhibition constraints, an acetaldehyde elimination system was constructed via introducing Sc ADH1 and Sc CR, which increased the molar conversion yield of glycine increased by 3.9-fold. Whole-cell catalysis gave a significantly higher conversion yield than crude enzyme preparations. A whole-cell co-expressing Pp LTA, Sc ADH1, and Sc CR was subsequently constructed. Finally, at 50 L scale, direct conversion of l -threonine fermentation broth afforded a glycine titer of 65.4 g/L within 24 h, with a molar conversion yield of 87.2%, In addition, 99.8% pure glycine was obtained with a yield of 76.6% via separation. Conclusions An efficient two-step process for converting glucose to glycine was developed and successfully validated. Evaluation at the 50 L scale demonstrated record-high glycine production performance. This work provides a promising technological route for the industrial manufacture of high-quality glycine. Graphical
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Yan Gao,Yunhong Zhang
In recent years, “brown carbon” (BrC), as an important contributor to light absorption and climate forcing as aerosols, has been one of the forefronts in the field of atmospheric research. Aqueous BrC aerosols can be formed through aqueous reactions of methylglyoxal (MG) with nitrogen compounds, such as glycine (Gly) and ammonium sulfate (AS). When exposed to nitrogen compounds for several days, aqueous carbonyl compound MG became absorbent and fluorescent in the ultraviolet and near visible regions, according to UV/Vis and fluorescence spectroscopies. Experiment results showed that optical absorption of two aqueous BrC solutions in the spectral range of 250–480 nm significantly increased with increasing reaction time. After the reactions of MG with Gly and AS, the product absorbance followed the order of MG-Gly>MG-AS. For H 2 O 2 oxidation photolysis, the atmospheric aqueous BrC showed the dynamic nature. Reaction kinetic, effective quantum yields and size distribution studies were conducted in the paper. Fluorescence lifetime values of the two BrC solutions were calculated. LC/MS analysis results clearly indicated that complicated organic compounds were formed in the reactions of MG with Gly and AS.
研究领域:
大气科学
·
环境化学
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高影响因子
ORIGINS OF LIFE AND EVOLUTION OF BIOSPHERES
·
2016
·Vol. 47
·期 4
·413-425
·
IF 1.141
Zhang Xianlong,Tian Ge,Gao Jing,Han Mei,Su Rui,Wang Yanxiang,Feng Shouhua
Submarine hydrothermal vents are generally considered as the likely habitats for the origin and evolution of early life on Earth. In recent years, a novel hydrothermal system in Archean subseafloor has been proposed. In this model, highly alkaline and high temperature hydrothermal fluids were generated in basalt-hosted hydrothermal vents, where H 2 and CO 2 could be abundantly provided. These extreme conditions could have played an irreplaceable role in the early evolution of life. Nevertheless, sufficient information has not yet been obtained for the abiotic synthesis of amino acids, which are indispensable components of life, at high temperature and alkaline condition. This study aims to propose a new method for the synthesis of glycine in simulated Archean submarine alkaline vent systems. We investigated the formation of glycine from ethanolamine under conditions of high temperature (80–160 °C) and highly alkaline solutions (pH = 9.70). Experiments were performed in an anaerobic environment under mild pressure (0.1–8.0 MPa) at the same time. The results suggested that the formation of glycine from ethanolamine occurred rapidly and efficiently in the presence of metal powders, and was favored by high temperatures and high pressures. The experiment provides a new pathway for prebiotic glycine formation and points out the phenomenal influence of high-temperature alkaline hydrothermal vents in origin of life in the early ocean.
研究领域:
生物化学
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地球科学
·
天体生物学
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高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2018
·
IF 2.559
Dan Zhao,Juan Chen,Ya Zhang,Hua-Bao Liao,Zhi-Feng Zhang,Yang Zhuang,Meng-Xian Pan,Jun-Chun Tang,Rui Liu,Yang Lei,Shu Wang,Xing-Ping Qin,Yu-Gong Feng,Yun Chen,Qi Wan
Glycine has been shown to protect against ischemic stroke through various mechanisms. Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) which antagonize Akt-dependent cell survival has been linked to neuronal damage. However, whether glycine has a neuroprotective property in intracerebral hemorrhage (ICH) was unknown. This study aimed to determine the protective effect of glycine in rats ICH. Adult male Sprague-Dawley (SD) rats were subjected to left striatum infusion of autologous blood. ICH animals received glycine (0.2–3 mg/kg, icv) at 1 h after ICH with or without pre-injection of Akt Inhibitor IV (100 μM, 2 μl, icv) 0.5 h prior to glycine treatment. Our results showed that in the perihematomal area PTEN was up-regulated in the early stage after ICH. However, glycine treatment decreased PTEN protein level and increased the phosphorylation level of AKT (p-AKT) in the perihematomal area. With the administration of glycine, neuronal death was significantly reduced and Evans blue leakage was alleviated as well as the brain edema after ICH. Moreover, hematoma volume was decreased and neurobehavioral outcome was improved. Nevertheless, Akt Inhibitor IV abolished the neuroprotective effects of glycine after ICH. Together, our findings demonstrate, for the first time, the protective role of glycine on ICH rats, and suggest that the neuroprotective effect of glycine was mediated through PTEN/Akt signal pathway.
研究领域:
神经科学
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药理学
·
分子生物学
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JOURNAL OF MEMBRANE SCIENCE
·
2020
·
IF 7.183
Zhitao Wu,Le Gao,Jie Wang,Feigang Zhao,Longlong Fan,Dan Hua,Susilo Japip,Jingran Xiao,Xueji Zhang,Shu-Feng Zhou,Guowu Zhan
In this work, we fabricated a series of composite membranes for nanofiltration by filtration-assisted assembly of graphene oxide (GO, with a thickness of ca. 2 nm) and graphitic carbon nitride (g-C 3 N 4 , with a thickness of ca. 5.2 nm) nanosheets with the assistance of glycine as a molecular linker to enhance their interactions. The effects of g-C 3 N 4 and glycine concentrations on the lamellar spacing of GO and membrane performance were subsequently investigated. Interestingly, experimental and characterization results showed that both g-C 3 N 4 and glycine could increase the interlayer spacing of pristine GO membranes, but the former reduced the dimensions of nanochannels while the latter led to enlarged channels. As compared with pristine GO membranes, the composite membranes resulted in faster water transportation without sacrificing solute retention. With further functionalization by introducing hyperbranched polyethyleneimine (HPEI) coating, we demonstrated that an integrated Gly-GO/g-C 3 N 4 membrane ( ca. 116 nm in thickness) exhibited excellent separation performance for various organic dye solutions under different operational conditions (feed concentration, pH, etc.). Long-term stability experiments showed that this membrane yielded 90%–93% dye rejection with only a slight decline in permeance over a 40 h testing period, indicating acceptable stability. The pure water permeance of the Gly-GO/g-C 3 N 4 membrane reached as high as 207 L h −1 m −2 bar −1 . These results indicate that the covalent modification of small molecules can improve the packing of GO nanosheets and lead to outstanding nanofiltration performance which has great potential applications in the field of water purification and separation.
研究领域:
材料科学
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环境工程
·
化学工程
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高影响因子
ACS Nano
·
2022
·
IF 18.027
Yu Liu,Yongkang An,Lu Wu,Jianguo Sun,Fangyu Xiong,Han Tang,Shulin Chen,Yue Guo,Lei Zhang,Qinyou An,Liqiang Mai
Zn metal is thermodynamically unstable in aqueous electrolytes, which induces dendrite growth and ongoing parasitic reactions at the interface during the plating process and even during shelf time, resulting in rapid battery failure and hindering the practical application of aqueous Zn ion batteries. In this work, glycine, a common multifunctional additive, is utilized to modulate the solvation shell structure and enhance the interfacial stability to guard the reversibility and stability of the Zn anode. Apart from partially replacing the original SO42– in the contact ion pair of Zn2+[H2O]5·OSO32– complexes to suppress the formation of Zn4(OH)6SO4·xH2O byproducts at the interface, glycine molecules can also form a water-poor electrical double layer on the zinc metal surface during resting and be further reduced to build in situ a ZnS-rich solid electrolyte interphase (SEI) layer during cycling, which further suppresses side reactions and the random growth of Zn dendrites in the whole process. As expected, the cycle life of the symmetrical cells reaches over 3200 h in glycine-containing electrolytes. In addition, the Zn//NVO full cell shows exceptional cycling stability for 3000 cycles at 5 A g–1. Given the low-cost superiority of glycine, the proposed strategy for interfacial chemistry modulation shows considerable potential in promoting the commercialization progress of aqueous batteries.
研究领域:
电化学
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材料科学
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高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Lu Wang,Lan Zhang,Baoxian Ye
Herein, an efficient method for in-situ assembly of silver (Ag) nanoparticles on glycine functionalized graphene (Gly-Gr) has been proposed, which only requires a simple one-step hydrothermal reaction. The microstructure of the material (Ag-Gly-Gr) was characterized by a series of methods, such as FT-IR, XRD, and TEM. For comparison, glycine functionalized graphene (Gly-Gr) was prepared by the same way without adding silver nitrate. The electrochemical sensor (Ag-Gly-Gr/GCE or Gly-Gr/GCE) was prepared by coating the glassy carbon electrode (GCE) surface with dispersive droplets of the related material. The outcomes of the electrochemical impedance test showed that the charge transfer resistance of Ag-Gly-Gr/GCE was less than that of Gly-Gr/GCE and GCE. Compared with the other two sensors, Ag-Gly-Gr/GCE showed a more sensitive response for bergenin. By studying the influence of the pH and sweep rate on the peak potential, the dynamics parameters were discussed to understand the electrochemical reaction mechanism of bergenin. The strategy for quantitative analysis of bergenin was further established at the working potential of 0.8 V (vs SCE). The peak current of bergenin displayed a linear dependence for concentration in 0.01–1 μM with a detection limit of 3.0 nM. Additionally, the analytical availability of Ag-Gly-Gr/GCE was demonstrated by determining bergenin in real-life samples.
研究领域:
材料科学
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分析化学
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纳米技术
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电化学
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高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2022
·Vol. 111
·期 3
·404-414
·
IF 4.854
Xiaoxue Wang,Jingliang Wu,Min Wang,Chunbo Lu,Wenfang Li,Qiao Lu,Yanying Li,Bo Lian,Bo Zhang
Recent efforts have focused on preparing drug-loaded hydrogel for wound healing. In order to obtain an ideal hydrogel dressing for skin wound repair, a carboxymethyl chitosan-gelatin hydrogel was prepared for co-delivery of SP (substance P) and DMOG (dimethyloxallyl glycine) by a chemical cross-linking method using genipin as the cross-linking agent. The synthesized hydrogels have good biocompatibility and physicochemical properties due to the low toxicity of the hydrogel material. The three-dimensional network structure of the hydrogels supports cell migration and proliferation, and the combination of SP and DMOG drugs exhibited strong effects on cell proliferation. Moreover, the co-loaded drug hydrogels could significantly promote wound healing in vivo, and provide a potential hydrogel for wound healing.
研究领域:
药学
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材料科学
·
生物医学工程
·
组织工程
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高影响因子
AICHE JOURNAL
·
2020
·Vol. 66
·期 11
·e17023
·
IF 3.519
Yaoming Wang,Xiaoli Wang,Haiyang Yan,Chenxiao Jiang,Liang Ge,Tongwen Xu
In this study, cleaner production of N -methylglycine (NMG), N , N -dimethylglycine (DMG), and N , N , N -trimethylglycine (TMG) with similar structures but different methylate groups was performed using bipolar membrane electrodialysis (BMED). The effects of the feed mass concentration and current density on the separation performance were intensively analysed in terms of the molecular size, molecular structure, ion concentration, and interaction between amino acids and membranes. The results indicated that the optimal recovery performance was achieved at a current density of 200 A/m 2 and feed mass concentration of 6%. Under the optimal conditions, the energy consumption and current efficiencies were 2.3 kWh/kg and 78% for NMG, 2.49 kWh/kg and 69.5% for DMG, and 3.52 kWh/kg and 39.6% for TMG, respectively. It was speculated a competition for water splitting occurs between the bipolar membranes and anion exchange membranes when BMED is used for the separation and purification of large-sized bioproducts.
研究领域:
生物工程
·
化学工程
·
环境科学
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高影响因子
EUROPEAN POLYMER JOURNAL
·
2025
·
IF 6.3
Qing Gao,Hailin Fu,Hui Liu,Pengfei Li,Jin Shi,Yao Lin,Ziyuan Song
Poly(sarcosine) (PSar)-based diblock copolypeptoid nanoparticles are promising alternatives to PEGylated polymeric nanocarriers. However, their preparation was limited by the slow polymerization kinetics and low assembly concentrations. Herein, we report the facile preparation of copolypeptoid nanoparticles through polymerization-induced self-assembly of N -substituted glycine N -carboxyanhydrides (NNCAs) in solvents with low polarity and weak hydrogen-bonding ability like dichloromethane (DCM). In contrast to the conventional polymerization in N , N -dimethylformamide (DMF), NNCAs were polymerized at an accelerated rate in DCM that minimized side reactions from water and other impurities. With isopentylamine-initiated PSar as the first stabilizing block, the polymerization of N -benzyl glycine N -carboxyanhydrides resulted in the formation of spherical or rod-like nano-assemblies at high solid contents (up to 15%) within several hours. We believe this work not only improves our basic understanding on NNCA polymerization behaviours, but also offers an efficient strategy to prepare drug nanocarriers with PSar corona, facilitating the downstream application studies.
研究领域:
材料科学
·
生物医学工程
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高影响因子
Biomass Conversion and Biorefinery
·
2021
·Vol. 13
·期 2
·583-592
·
IF 4.987
Chen Guozheng,Dong Junjing,Wan Jinquan,Ma Yongwen,Wang Yan
In response to deal with the increasingly serious environmental problems and shortage of fiber raw materials, biological enzyme pretreatment is an effective way to replace a large number of chemical additives to improve the properties of waste paper fibers. Fiber characterization of old corrugated container bleached pulp with laccase and glycine (Lac/Gly) pretreatment was investigated by means of Fourier transform infrared spectroscopy (FTIR), headspace gas chromatography (HSGC), fiber quality measurements (FQA), X-ray diffraction method (XRD), and atomic force microscope (AFM). Results showed that, compared with the control pulp, the whiteness and brightness of the Lac/Gly-treated pulp increased by 16.17% and 7.41%, respectively. And the FTIR showed that Lac/Gly pretreatment promotes the effective removal of lignin by hydrogen peroxide (H 2 O 2 ) bleaching. The content of carboxyl groups in pulp increased remarkably by 21.92%. The paper physical analysis showed that the paper strength properties have improved remarkably. The fiber quality analyses indicated that the fiber length, coarseness, and curl index changed a little. The XRD analysis showed that the crystallinity decreased by 5.83% due to Lac/Gly treatment. The AFM analysis showed that through Lac/Gly treatment, the lignin and extracts over the fiber surface are decreased significantly.
研究领域:
制浆造纸工程
·
环境科学
·
生物技术
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高影响因子
JOURNAL OF CLUSTER SCIENCE
·
2020
·Vol. 32
·期 6
·1553-1561
·
IF 1.731
Jia Jinliang,Liu Qingle,Jin Xiaoyong,Xu Hanhong
Fluorescent gold nanoclusters (Au NCs) have attracted considerable interest in biological application. Here, we reported a novel Au NCs with blue fluorescence, conjugated with glycine (Gly) and rotenone (R). The structure and properties of Au–BSA–Gly–R conjugates were characterized by transmission electron microscopy (TEM), UV–visible, fluorescence spectroscopy, Fourier transform infrared spectroscopy (FTIR) and Zeta potential measurement. The results of fluorescent microscopic imaging revealed that the Au NCs with different ligands were capable of being selectively uptaken by Spodoptera frugiperda (Sf9) cells. Furthermore, the gold content of Au–BSA–Gly–R in Sf9 cells was 2.2 and 2.6 times higher than that of Au–BSA and Au–BSA–R, respectively. It illustrated that nanoparticles after conjugated with Gly could gain specificity to intracellular transportation. This study indicated that the Au NCs after mediated by Gly not only had great potential for applications in insect cell imaging, but also could play an important role of amino acid for agrochemicals delivery in agricultural fields.
研究领域:
纳米技术
·
生物科学
·
农业化学
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高影响因子
危险品化学品经营许可证（带存储）