CHINESE JOURNAL OF POLYMER SCIENCE
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2015
·Vol. 33
·期 4
·587-596
·
IF 1.835
Song Zhi-ming,Shi Bo,Ding Jian-xun,Zhuang Xiu-li,Zhang Xiao-nan,Fu Chang-feng,Chen Xue-si
An electrospun poly(lactide- co -glycolide) (PLGA) membrane was prepared and used to perform the anti-adhesion of Achilles tendon. Throughout the experiments, the membrane showed an appropriate degradation rate, and the pH values of degradation media were maintained at around 7.4. Simultaneously, the excellent biocompatibility of the membrane in vitro and in vivo was confirmed by live/dead and histopathological analyses. Meanwhile, the membrane can reduce tendon adhesion significantly and promote functional recovery effectively. The encouraging results were further demonstrated by hematoxylin and eosin (H&E), and Masson’s trichrome stainings, and type I collagen immunohistochemical analysis. It was concluded that the model treated with the electrospun PLGA membrane was significantly better with respect to the adhesion prevention and tissue repair than that without treatment. Considering the results of degradation and adhesion prevention efficacy, the electrospun PLGA membrane would be a great candidate for the prevention of postoperative tendon adhesion.
研究领域:
生物医学工程
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材料科学
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组织工程
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骨科手术
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产品关联
DOI
高影响因子
BIOMACROMOLECULES
·
2019
·
IF 5.667
Shue Jin,Fuhua Sun,Qin Zou,Jinhui Huang,Yi Zuo,Yubao Li,Suping Wang,Lei Cheng,Yi Man,Fang Yang,Jidong Li
The purpose of this study was to fabricate a low-immunogenicity fish collagen (FC) and bioactive nanohydroxyapatite (n-HA) enhanced poly(lactide-co-glycolide) (PLGA) nanofibrous membrane for guided bone regeneration (GBR) via electrospinning. The physicochemical properties and morphology study revealed that FC and n-HA particles were homogeneously dispersed in the PLGA fibrous matrix. Notably, the formation of enhanced polymeric chain network due to the interaction between FC and PLGA significantly improved the tensile strength of the PLGA membrane. The incorporation of FC altered the degradation behavior of fibers and accelerated the degradation rate of the PLGA-based membranes. Moreover, the membranes exhibited favorable cytocompatibility with bone mesenchymal stem cells (BMSCs) and human gingiva fibroblasts (HGF) cells. More importantly, the optimized membrane satisfied the requirements of the ‘Biological evaluation of medical devices’ during the incipient biosafety evaluation. All the results indicate that this composite fibrous membrane exhibits significant potential for guided bone or tissue regeneration.
研究领域:
生物材料
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组织工程
·
再生医学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Materials
·
2015
·Vol. 8
·期 3
·1009-1026
·
IF 2.651
Juan Wang,Dongsong Li,Tianyi Li,Jianxun Ding,Jianguo Liu,Baosheng Li,Xuesi Chen
Surface coating is the simplest surface modification. However, bioactive molecules can not spread well on the commonly used polylactone-type skeletons; thus, the surface coatings of biomolecules are typically unstable due to the weak interaction between the polymer and the bioactive molecules. In this study, a special type of poly(lactide-co-glycolide) (PLGA)-based scaffold with a loosened skeleton was fabricated by phase separation, which allowed gelatin molecules to more readily diffuse throughout the structure. In this application, gelatin modified both the internal substrate and external surface. After cross-linking with glutaraldehyde, the surface layer gelatin was tightly bound to the diffused gelatin, thereby preventing the surface layer gelatin coating from falling off within 14 days. After gelatin modification, PLGA scaffold demonstrated enhanced hydrophilicity and improved mechanical properties (i.e., increased compression strength and elastic modulus) in dry and wet states. Furthermore, a sustained release profile of recombinant human bone morphogenetic protein-2 (rhBMP-2) was achieved in the coated scaffold. The coated scaffold also supported thein vitroattachment, proliferation, and osteogenesis of rabbit bone mesenchymal stem cells (BMSCs), indicating the bioactivity of rhBMP-2. These results collectively demonstrate that the cross-linked-gelatin-coated porous PLGA scaffold incorporating bioactive molecules is a promising candidate for bone tissue regeneration.Keywords:biomaterials;bone tissue engineering;gelatin;surface coating;recombinant human bone morphogenic protein-2
研究领域:
生物医学工程
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组织工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
Biomedical Materials
·
2010
·Vol. 5
·期 6
·065016
·
IF 1.963
Deming Xie,Huamei Huang,Keith Blackwood,Sheila MacNeil
Both chitosan and polylactide/polyglycolide have good biocompatibility and can be used to produce tissue engineering scaffolds for cultured cells. However the synthetic scaffolds lack groups that would facilitate their modification, whereas chitosan has extensive active amide and hydroxyl groups which would allow it to be subsequently modified for the attachment of peptides, proteins and drugs. Also chitosan is very hydrophilic, whereas PLGA is relatively hydrophobic. Accordingly there are many situations where it would be ideal to have a copolymer of both, especially one that could be electrospun to provide a versatile range of scaffolds for tissue engineering. Our aim was to develop a novel route of chitosan-g-PLGA preparation and evaluate the copolymers in terms of their chemical characterization, their performance on electrospinning and their ability to support the culture of fibroblasts as an initial biological evaluation of these scaffolds. Chitosan was first modified with trimethylsilyl chloride, and catalyzed by dimethylamino pyridine. PLGA-grafted chitosan copolymers were prepared by reaction with end-carboxyl PLGA (PLGA–COOH). FT-IR and1H-NMR characterized the copolymer molecular structure as being substantially different to that of the chitosan or PLGA on their own. Elemental analysis showed an average 18 pyranose unit intervals when PLGA–COOH was grafted into the chitosan molecular chain. Differential scanning calorimetry results showed that the copolymers had different thermal properties from PLGA and chitosan respectively. Contact angle measurements demonstrated that copolymers became more hydrophilic than PLGA. The chitosan-g-PLGA copolymers were electrospun to produce either nano- or microfibers as desired. A 3D fibrous scaffold of the copolymers gave good fibroblast adhesion and proliferation which did not differ significantly from the performance of the cells on the chitosan or PLGA electrospun scaffolds. In summary this work presents a methodology for making a hybrid material of natural and synthetic polymers which can be electrospun and reacts well as a substrate for cell culture.
研究领域:
生物材料
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组织工程
·
高分子化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF POLYMER ANALYSIS AND CHARACTERIZATION
·
2019
·
IF 1.426
Jianglong Duan,Zhihua Zhou,Tianlong Huang,Wenjuan Liu,Yanmin Zhao,Wei Wu,Xiaofei Li,Jianjun Fang
Three-dimensional biodegradable porous scaffolds play an important role in tissue engineering. The degradable scaffold material, based on 1,4-butanediamine-modified poly(lactide-co-glycolide) (BMPLGA), nano-bioactive glass (NBAG), and nano-β-tricalciumphosphate (β-TCP), was prepared by a solution-casting/salt-leaching method. The biological properties were studied by using cell cytotoxicity, von Kossa staining, alkaline phosphatase activity, hemolytic test, acute toxicity, and genetic toxicity test. The MTT results indicated that the BMPLGA/NBAG-β-TCP materials did not show any cytotoxicity. The result of von Kossa staining showed that the introduction of the NBAG and β-TCP promoted fibroblastic differentiation and improved the mineral deposition of the BMPLGA matrix. In addition, the presence of NBAG and β-TCP in the composite further enhanced the ALP activity in comparison with the sole BMPLGA material. The hemolytic potential showed that the nanocomposite scaffolds were non-hemolytic. The BMPLGA/NBAG-β-TCP scaffolds showed no acute systemic toxicity or mutagenic action. Therefore, the results indicated the BMPLGA/NBAG-β-TCP nanocomposite scaffold could be considered as a potential bone tissue engineering implant.
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DOI
高影响因子
Journal of Macromolecular Science Part B-Physics
·
2019
·
IF 1.204
Wei Wu,Zhihua Zhou,Wenjuan Liu,Yunhui Zhao,Yanmin Zhao,Tianlong Huang,Xiaofei Li,Jianjun Fang
A random terpolymer of poly(L-lactide-co-glycolide-co-ε-caprolactone) (PLLGC) was synthesized from L-lactide, glycolide and ε-caprolactone. The in-vitro hydrolytic degradation behavior of the PLLGC terpolymer was investigated as a function of the degradation time; the results showed that the degradation rate of PLLGC was lower than that of PLGA due to a reduction in the acidic degradation products. Therefore, we suggest the degradation rate of the PLGA could be controllable by introduction of ε-caprolactone into the PLGA martrix, while the physical properties (mechanical, etc.) were superior to PLGA.
研究领域:
高分子科学
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生物材料
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生物医学工程
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药物递送系统
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DOI
高影响因子
POLYMER BULLETIN
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2015
·Vol. 72
·期 6
·1531-1543
·
IF 1.438
Zhou Zhihua,Huang Huihua,Huang Tianlong,Peng Cheng,Zhou Hu,Liu Qingquan,Zeng Wennan,Liu Lihua,Cao Dafu,He Siliang,Xiang Liujiao,Yan Hua
Novel maleated poly( d , l -lactide- co -glycolide) (MPLGA) was melt copolymerized directly from maleic anhydride, d,l -lactide and glycolide monomers with stannous octoate as catalyst and benzoyl peroxide as initiator. FTIR, 1 H NMR, GPC and maleation content were employed to qualitatively characterize the synthesized MPLGA. The effects of the catalyst dosage, reaction time and reaction temperature on weight average molecular mass, polydispersity index and maleation content of MPLGA were also investigated. The results indicated that the novel MPLGA was successfully obtained using melting-copolymerization method. MPLGA, with a weight average molecular mass of about 5.39 × 10 4 Da, a polydispersity index of 1.51 and a maleation content of 7.1 wt%, was obtained when polymerization was conducted with catalyst dosage of 0.03 wt% for 36 h at 140 °C. The results indicated that the introduction of maleic anhydride into PLGA may offer new and important options for biomaterial applications.
研究领域:
材料科学
·
高分子化学
·
生物材料
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产品关联
DOI
高影响因子
Nanomedicine
·
2018
·
IF 5.005
Shuang Xie,Yan C Gong,Xiang Y Xiong,Zi L Li,Yue Y Luo,Yu P Li
Aim: To explore the better efficacy of targeted folic acid (FA)-Pluronic 85-poly(lactide-co-glycolide) (FA–P85–PLGA) polymersome in oral insulin delivery. Materials & methods: The cytotoxicity of the polymers, in vitro qualitative and quantitative cellular uptake and the internalization mechanism of insulin-loaded FA–P85–PLGA and PLGA–P85–PLGA polymersomes were studied with the human colon adenocarcinoma cells (Caco-2 cells). Their pharmacodynamics and pharmacokinetics properties were also studied with diabetic rats. Results & conclusion: Polymersomes have shown good biocompatibility. Polymersomes are mainly localized within the cytoplasm of Caco-2 cells from fluorescence microscopy images. FA–P85–PLGA exhibited higher cellular uptake than PLGA–P85–PLGA polymersomes and free fluorescein isothiocyanate-labeled insulin (FITC–insulin) did. The uptake process of targeted polymersomes included clathrin- and caveolae-mediated endocytosis, macropinocytosis and the folate receptor-mediated endocytosis. Insulin-loaded FA–P85–PLGA showed better hypoglycemic effects than insulin-loaded PLGA–P85–PLGA.
研究领域:
药学
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药物递送系统
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生物医学工程
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS APPLICATIONS
·
2023
·
IF 2.9
Tian Yi Wu,Wen Jie Cao,Zi Ling Li,Yan Chun Gong,Xiang Yuan Xiong
Drugs with different solubility can be selectively embedded into polymersomes with the hydrophilic core and hydrophobic bilayer. Novel folate-targeted Pluronic/poly (D,L-lactide-b-glycolide) polymersomes were constructed and used for the co-delivery of paclitaxel (PTX) and doxorubicin (DOX) to improve their inhibitory effect over cancer cells. The particle size of blank polymersomes was mainly distributed below 125 nm. The release of PTX and DOX from polymersomes showed an initial burst release followed by a sustained and slow release. The in vitro cytotoxicity data showed that the targeted co-loaded polymersomes (PTX&DOX FA-Ps) exhibited better inhibitory effect than single-loaded polymersomes and free drugs did. Furthermore, PTX&DOX FA-Ps showed the synergistic therapeutic effect over OVCAR-3 cancer cells. The cellular uptake results also showed that folate modified polymersomes had excellent targeting performance. Therefore, the folate-targeted Pluronic/poly (D,L-lactide-b-glycolide) polymersomes have potential application value as novel drug carriers to co-deliver PTX and DOX.
研究领域:
药学
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纳米技术
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癌症研究
·
药物递送系统
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DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL SCIENCES
·
2026
·Vol. 115
·期 9
·104414
·
IF 3.9
Shulin Chen,Jiahui Ma,Yongchao Ma,Jiale Hu,Jianxi Liu,Lulu Zhu,Yunqing Xia,Hao Jiang,Li Shen,Ying Xue,Wanhui Liu,Lixiao Xu
Poly(lactide-co-glycolide) polymers (PLGAs) are indispensable biodegradable matrices for long-acting parenteral (injectable) microsphere formulations. To modulate drug release kinetics and mitigate burst release in these complex injectable systems, PLGAs with varying lactide:glycolide (L:G) ratios are often employed in combination. While essential for ensuring batch-to-batch consistency and developing generic long-acting injectables, characterizing the individual properties of mixed PLGAs remains technically challenging. This study establishes a simple and accurate method to separate and characterize PLGA 7525 5A and PLGA 5050 2.5A within risperidone microspheres based on their solubility differences (semi-solvent principle). Trichloroethylene was identified as the optimal separation solvent, with n-propyl acetate serving as the washing solvent. The method was optimized by investigating parameters such as solvent volume, temperature, and mechanical assistance, with the addition of glass beads proving critical for effective separation. Molecular weight (Mw) analysis was performed using Gel Permeation Chromatography (GPC) on Agilent PLgel columns (connected in series) with tetrahydrofuran (THF) as the mobile phase. The method demonstrated excellent linearity ( R 2 = 0.9998) within the calibration range of 1220–554,000 g ·mol⁻¹ (Polystyrene equivalent). Precision was high, with RSD values of 0.33% (n = 6) for the supernatant Mw and 2.1% (n = 6) for the sediment Mw. The method's discriminatory ability was validated using simulated single-polymer degradation and mixed degraded microspheres. In conclusion, this method enables independent quality control of individual PLGA components within complex injectable matrices, providing a vital analytical tool for the key characteristic evaluation and bioequivalence assessment of complex generic parenteral formulations.
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产品关联
DOI
PubMed
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2018
·Vol. 29
·期 6
·1684-1696
·
IF 2.137
Xiaoyu Chen,Xiaomeng Wu,Zhongyong Fan,Qinghua Zhao,Qing Liu
The macroinitiator of poly(1,3-trimethylene carbonate) (PTMC) with number-average molecular weight ( ) of 9.6 × 10 3 g mol −1 was synthesized by ring-opening polymerization at 120°C. Then, the novel terpolymer P(TMC- b -( L LA- ran -GA)) consisting of PTMC homopolymer segment attached with various monomer molar ratios of L -lactide ( L LA) and glycolide (GA) random copolymerization block was prepared with about 5.0 × 10 4 g mol −1 by ring-opening polymerization in bulk at 140°C. The tailored molecular structures of P(TMC- b -( L LA- ran -GA)) were characterized by 1 H nuclear magnetic resonance, 13 C NMR, FTIR, and gel permeation chromatography, and chain microstructure analysis was performed in detail with 13 C NMR spectroscopy. The effect of GA units on the thermal and crystallization behaviors, mechanical properties, as well as biodegradability of terpolymers was investigated by differential scanning calorimetry, wide-angle X-ray diffraction, stress-strain measurements, and in vitro tests in comparison with corresponding poly(trimethylene carbonate- block - L -lactide) copolymer P(TMC- b - L LA). The results show that amorphous PTMC segments have a significant effect on condensed state behavior of the terpolymers, and the incorporation of GA units strongly decreases the crystallinity and crystallization ability of L LA segment within terpolymers because of more random L LA-GA sequence and shorter average L LA block length. Meanwhile, the toughness of materials is greatly improved, and in vitro degradation is also accelerated. Peripheral vascular stents were 3D printed for the first time and met the requirements for application. The results show totally biodegradable terpolymers with unique molecular structure, and modifiable properties are promising new biomaterials with advanced performance for biomedical application.
研究领域:
生物材料学
·
高分子化学
·
医学工程
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DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2016
·
IF 3.782
Hui Zhang,Jiangkang Xu,Liang Xing,Jianbo Ji,Aihua Yu,Guangxi Zhai
Doxorubicin (DOX) is one of the most common chemotherapeutic agents for the treatment of various cancers, but its clinical usage is limited by dose-dependent cardiotoxicity. We have recently synthesized a series of Chondroitin sulfate/poly ( d , l -lactideco-glycolide) block copolymers (Chs- b -PLGA) with different length of hydrophobic block by an end-to-end coupling strategy. The structure of the amphiphilic block copolymers with low critical micelle concentration (∼28 mg/L) were confirmed by 1 H NMR. The copolymers could self-assemble into stable micelles in aqueous environment with homogeneous size distribution and negative zeta potential . The hemolytic study indicated their excellent blood compatibility and potential application for intravenous administration. DOX can be efficiently encapsulated by the Chs- b -PLGA micelles. The DOX-loaded micelles showed a sustained and pH dependent drug release behavior. The cytotoxicity assay showed no associated toxicity with blank micelles while concentration-related cell inhibition with DOX-loaded micelles. Moreover, fast cellular uptake of DOX-loaded micelles was observed by fluorescent microscope. In vivo pharmacokinetics study showed that the Chs- b -PLGA micelles could significantly prolong the blood circulation time of DOX. The maximum tolerated dose (MTD) of DOX-loaded micelles in Kunming mice was about 2-fold higher of the MTD of free DOX, indicating a high tolerance of the DOX-loaded micelles. In conclusion, the Chs- b -PLGA micelles would be a potentially useful drug delivery system for cancer therapy.
研究领域:
药剂学
·
药物递送
·
纳米医学
·
高分子科学
·
癌症治疗
·
生物医学工程
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）