MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 2
·98
·
IF 5.3
Cui Mingyu,Shi Yuqing,Xin Jianwei,Zhang Yuqi,Wang Ji-Jiang
A Cu 2+ -coordination crosslinked two-dimensional photonic crystal hydrogel (PN-2DPCH-Cu 2+ ) was constructed, in which a polystyrene two-dimensional photonic crystal (PS-2DPC) was embedded in a Cu 2+ -crosslinked poly(acrylamide˗ co ˗acrylic acid˗ co ₋N-vinyl imidazole) hydrogel network. The introduction of Cu 2+ ions was mainly based on the coordination reaction of Cu 2+ and N atoms of the imidazole rings on the hydrogel chains. The constructed PN-2DPCH-Cu 2+ smart photonic hydrogel sensor achieved highly sensitive and simple detection of histidine (His) just using a laser pointer and a ruler. The coordination competition of Cu 2+ to His and N atoms of the imidazole rings drove the hydrogel’s response. In the presence of His, the PN-2DPCH-Cu 2+ sensor swells obviously because the stronger coordination between Cu 2+ ions and histidine compared to imidazole rings removed the coordination-crosslinked Cu 2+ from the hydrogel, thus decreasing its crosslinking density. The swollen hydrogel increased the microsphere spacing of the embedded PS-2DPC, which can be acquired by measuring the Debye diffraction ring diameter of PS-2DPC. The microsphere spacing increments were linearly changed when the His concentrations were in the ranges 0.01–0.03 µM, 0.05–1 µM and 10–500 µM, and the limit of detection was 9.6 nM. The sensor showed high selectivity to His, good anti-interference and stability, and the response can be achieved in 20 min. The good recoveries and relative standard deviations from determining His contents in milk and beverage using our constructed PN-2DPCH-Cu 2+ sensor demonstrated its accuracy and reliability. The coordination competition strategy of driving hydrogel to response can extend to develop other analytes sensitive hydrogel sensors by choosing suitable polymerizable monomers and metal ions. Graphical
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高影响因子
NANO
·
2020
·
IF 1.566
Zhifeng Cai,Xiu Yin,Jingling Fang,Jie Zhao,Tianqi Wu,Erxiao Hao,Fang Tian,Caifeng Zhang
In this contribution, a one-pot synthesis method possessing the advantages of simple, green and low-cost had been researched for the preparation of L-histidine-stabilized Cu nanoclusters (Cu NCs). Subsequently, the structure and optical properties of as-prepared Cu NCs were studied by using Fourier transform infrared (FTIR), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), fluorescence spectroscopy and UV-Vis absorption spectroscopy. TEM image of the Cu NCs showed high dispersion with an average diameter of 2.0 nm. Fluorescence spectrum displayed that the Cu NCs emitted green fluorescence (emission wavelength of 492 nm) under excitation wavelength of 393 nm. Moreover, the as-synthesized Cu NCs illustrated excellent performances, such as good water solubility, UV stability and high-salt resistance. Interestingly, the fluorescence intensity of as-prepared Cu NCs was obviously quenched in the presence of fluazinam. Under optimal conditions, the relative fluorescence intensity was linear with the fluazinam concentrations from 1 to 40 μ M, with a detection limit of 0.25 μ M. Eventually, the fluorescence sensor was successfully used to determine fluazinam in real water samples.
研究领域:
纳米材料
·
分析化学
·
环境监测
·
荧光传感
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CHEMISTRY-A EUROPEAN JOURNAL
·
2012
·Vol. 19
·期 2
·501-508
·
IF 5.925
Jianwei Nai,Zhengbo Chen,Haopeng Li,Fangyuan Li,Yang Bai,Lidong Li,Lin Guo
As the properties of nanomaterials are strongly dependent on their size, shape and nanostructures, probing the relations between macro-properties and nanostructures is challenging for nanoscientists. Herein, we deliberately chose three types of Ni(OH) 2 with hexagonal, truncated trigonal, and trigonal hourglass-like nanostructures, respectively, as the electrode modifier to demonstrate the correlation between the nanostructures and their electrocatalytic performance towards L -histidine. It was found that the hexagonal hourglass-like Ni(OH) 2 sample had the best electrocatalytic activity, which can be understood by a cooperative mechanism: on one hand, the hexagonal sample possesses the largest specific surface area and the tidiest nanostructure, resulting in the most orderly packing on the electrode surface; on the other hand, its internal structure with the most stacking faults would generate a lot of unstable protons, leading to an enhanced electronic conductivity. The findings are important because they provide a clue for materials design and engineering to meet a specific requirement for electrocatalysis of L -histidine, possibly even for other biomolecules. In addition, the hexagonal Ni(OH) 2 -based biosensor shows excellent sensitivity and selectivity in the determination of L -histidine and offers a promising feature for the analytical application in real biological samples. Graphical Structure-dependent performance : Three different types of Ni(OH) 2 with hexagonal, truncated trigonal, and trigonal hourglass-like nanostructures, respectively, were deliberately chosen as electrode modifiers to demonstrate the correlation between the structures and their electrocatalytic performance toward L -histidine (see figure). The electrocatalytic activity of these unique nanostructures depends not only their external architecture, but also on their relevant internal degree of crystallinity.
研究领域:
纳米材料
·
电化学
·
催化
·
生物传感器
·
材料科学
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WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2024
·Vol. 40
·期 8
·1-11
·
IF 4
Qiao Cailin,Yang Suping,Ma Yi,Wen Li,Chu Chengxiang,Luo Huibo,Luo Xiaogang,Hou Changjun,Huo Danqun
Saccharomyces cerevisiae , the primary microorganism involved in ethanol production, is hindered by the accumulation of ethanol, leading to reduced ethanol production. In this study, we employed histidine-modified Fe 3 O 4 nanoparticles (His-Fe 3 O 4 ) for the first time, to the best of our knowledge, as a method to enhance ethanol yield during the S. cerevisiae fermentation process. The results demonstrated that exposing S. cerevisiae cells to Fe 3 O 4 nanoparticles (Fe 3 O 4 NPs) led to increased cell proliferation and glucose consumption. Moreover, the introduction of His-Fe 3 O 4 significantly boosted ethanol content by 17.3% ( p < 0.05) during fermentation. Subsequent findings indicated that the increase in ethanol content was associated with enhanced ethanol tolerance and improved electron transport efficiency. This study provided evidence for the positive effects of His-Fe 3 O 4 on S. cerevisiae cells and proposed a straightforward approach to enhance ethanol production in S. cerevisiae fermentation. The mediation of improved ethanol tolerance offers significant potential in the fermentation and bioenergy sectors.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-19
·
IF 14.7
Lv Keyu,Chen Shuai,Xu Xulin,Chiu Joyce,Wang Haoqing J.,Han Yunyun,Yang Xiaodan,Bowley Sheryl R.,Wang Hao,Tang Zhaoming,Tang Ning,Yang Aizhen,Yang Shuofei,Wang Jinyu,Jin Si,Wu Yi,Schmaier Alvin H.,Ju Lining A.,Hogg Philip J.,Fang Chao
The essence of difference between hemostasis and thrombosis is that the clotting reaction is a highly fine-tuned process. Vascular protein disulfide isomerase (PDI) represents a critical mechanism regulating the functions of hemostatic proteins. Herein we show that histidine-rich glycoprotein (HRG) is a substrate of PDI. Reduction of HRG by PDI enhances the procoagulant and anticoagulant activities of HRG by neutralization of endothelial heparan sulfate (HS) and inhibition of factor XII (FXIIa) activity, respectively. Murine HRG deficiency ( Hrg −/− ) leads to delayed onset but enhanced formation of thrombus compared to WT . However, in the combined FXII deficiency ( F12 −/− ) and HRG deficiency (by siRNA or Hrg −/− ), there is further thrombosis reduction compared to F12 −/− alone, confirming HRG’s procoagulant activity independent of FXIIa. Mutation of target disulfides of PDI leads to a gain-of-function mutant of HRG that promotes its activities during coagulation. Thus, PDI-HRG pathway fine-tunes thrombosis by promoting its rapid initiation via neutralization of HS and preventing excessive propagation via inhibition of FXIIa.
研究领域:
血液学
·
分子生物学
·
血栓与止血
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Tong Li,Hui Li,Jia Chen,Yongliang Yu,Shuai Chen,Jianhua Wang,Hongdeng Qiu
Chiral enantiomers, especially the enantiomers of chiral drugs often exhibit different pharmacological activity, metabolism and toxicity, thus it is of great research significance to scientifically and reasonably develop single chiral drugs with low toxicity and high efficiency. Among them, high performance liquid chromatographic techniques based on chiral stationary phases (CSPs) has become one of the most attractive methods used to evaluate the enantiomeric purity of single-enantiomers compound of pharmacological relevance. In this work, pillar[5]arene functionalized with L- and D-histidine, respectively, were modified on the surface of mesoporous silica as novel chiral stationary phases called L / D His-BP5-Sil. Notably, L / D -histidine had the characteristics of low steric hindrance and easy derivatization. Although the π-π interaction of imidazole group was weaker than that of benzene ring, the benzene ring bonding imidazole-conjugated ring in the structure produced better enantioseparation effect. The results showed that L / D His-BP5-Sil can separate a variety of complex structural enantiomers with excellent reproducibility, thermal stability and separation performance. Hence, the unique advantage of the highly selective separation of L / D His-BP5-Sil provides new insights into the enantioseparation field.
研究领域:
药物化学
·
分析化学
·
材料科学
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高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Xiaoting Zhao,Jiayi Lu,Wenzhong Niu,Qidong Wang,Yanxia Qi,Lu Shi,Yuchun Qiao,Jizhen Shang,Limin Lu,Shuai Li,Hua Wang
Flower-like copper nanocomposites (Cu NFs) were synthesized through the coordination-driven assembly of Cu 2+ with protoporphyrin IX (Pp IX) for electrochemically sensing histidine (His) in blood. More interestingly, the Cu NFs-modified electrode could exhibit characteristic electrochemical peaks of CuCl at a lower and more favorable potential, which is practically advantageous. Especially, the CuCl signals would undergo a selective enhancement in the presence of His because of the specific coordination between copper ions and the imidazole moiety in His. This mechanism forms the basis of a proposed electrochemical strategy for highly selective His detection, exhibiting a linear range of 0.05 μM–100 μM. The limit of detection (LOD) for this method was calculated to be about 0.014 μM. Furthermore, the newly developed electrochemical strategy was applied to detect His in blood samples, showing analytical performances comparatively superior to those of the documented methods. This low-potential electrochemical detection strategy with enhanced signal output shows significant potential for use in the disease diagnostics and food safety analysis fields.
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ACS Applied Nano Materials
·
2018
·
IF 0
Chengbao Liu,Peng Du,Haichao Zhao,Liping Wang
Graphene-based carbonaceous materials have aroused great attention among the material protection fields due to their excellent impermeability. However, graphene nanosheets tend to aggregate in a polymer matrix and trigger microgalvanic corrosion, which potentially aggravates metal deterioration. Herein, we present a facile strategy to improve the protective property of graphene by tailoring graphene oxide with l-histidine molecules. The resultant functionalized graphene nanomaterials, without direct connections, can be well-dispersed in a polymer matrix. Electrochemical impedance measurements demonstrated that embedding a small percentage of well-dispersed functionalized graphene in epoxy coatings significantly enhanced the impermeable properties of the as-prepared composite coatings. The probability of galvanic corrosion of graphene with metal was largely inhibited and verified through the scanning vibrating electrode technique. The protection mechanism of composite coatings is interpreted as the barrier property of graphene nanosheets by suppressing the diffusion of corrosive species and the isolate function of l-histidine at the edge of graphene lamellae via increasing the electrical resistance.
研究领域:
材料科学
·
电化学
·
表面工程
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Xian-song Wang,Shen Zhang
In this study, histidine-protected copper nanoclusters (Cu NCs@His) were established by using a one-pot method, which histidine and ascorbic acid were applied as the template and reducing agent, respectively. The as-developed Cu NCs@His endued green emission wavelength at 494 nm with the excitation of 378 nm. The Cu NCs@His exhibited green fluorescence under UV light (365 nm). Using Cu NCs@His as a pattern nanosensor, the fluorescent “turn off” mechanism was fabricated for the determination of chlortetracycline in the light of the linear decrease of fluorescence intensities around 494 nm. The chlortetracycline conducted as a quencher, leading to reveal an excellent linear relationship between ln(F 0 /F) of Cu NCs@His and chlortetracycline concentrations with the range of 0.5–200 μM, and the detection limit was 0.876 μM. The fluorescence quenching of Cu NCs@His revealed excellent selectivity for chlortetracycline over other potential interfering substances in the human body. This strategy was exhibited to be a convenient sensing platform for the detection of chlortetracycline in real medical samples, which could unfold a brand new and direct system for the sensing of chlortetracycline in real samples.
研究领域:
分析化学
·
生物医学
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Ruihong Yao,Zhijian Li,Panpan Huo,Congcong Gong,Gang Liu,Chunhong Zheng,Shouzhi Pu
In this study, we constructed zinc based metal–organic framework functionalized by L-histidine (His@ZiF-8). The His@ZiF-8 had high porosity, large surface area, abundant carboxyl and amino group, which has been found to greatly enhance the aggregation-induced emission (AIE) of tetracycline (TC). After the His@ZiF-8 enrichment with TC, the TC exhibited strong fluorescence emission peak at 565 nm based on AIE. Under the optimal conditions, the fluorescence intensity of TC exhibited a good linear relationship with TC concentration within 0.1–80 μM with a low limit of detection of 28.6 nM. Antibiotic analogs such as neomycin, chloramphenico, ampicillin, kanamycin, and erythromycin have no obvious interference. In addition, the developed method was successfully used to the detection of TC in milk, river water, and honey.
研究领域:
材料科学
·
分析化学
·
环境科学
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高影响因子
RSC Advances
·
2015
·Vol. 5
·期 67
·54182-54187
·
IF 3.84
Zhiqiang Cheng,Fanli Zhang,Wei Liu,Liying Cui,Lijuan Kang
In this paper, we have prepared an antibacterial material of PVA/L-histidine (L-H)/Ag fibers by electrospinning in a double nozzle for the first time. The composite fibers were crosslinked by the mixture of glutaraldehyde and hydrogen chloride gas. After that, the fibers were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDS) and FT-IR spectra. The results indicated the size of silver particles distributed on the fiber was in the nanoscale below 10 nm, and OH groups have significantly decreased but the CO groups increased after crosslinking treatment by the gas phase. Simultaneously, the mechanical property for the membrane also greatly improved and the maximum strain and toughness reached 225.01% and 11.31 J g−1 respectively. Lastly, in order to investigate the antibacterial property of the membrane, we chose E. coli and Staphylococcus as inhibition targets, which showed the excellent antibacterial property for blocking the growth and destroying the structure of the bacteria.
研究领域:
材料科学
·
生物学
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PHYTOPATHOLOGY
·
2019
·Vol. 109
·期 3
·
IF 3.264
Jinli Li,Fuxing Zhu,Jianhong Li
Histidine kinases (HK) are implicated in virulence, vegetative mycelial growth, and osmotic and oxidative responses in pathogenic fungi. Our previous work showed that transcriptional levels of the group III HK gene Sshk are higher in field dimethachlone-resistant isolates of Sclerotinia sclerotiorum compared with sensitive isolates. However, it is not clear whether the overexpression of Sshk is the major mechanism for resistance to dimethachlone. In this study, we constructed Sshk silencing and overexpression vectors and assessed dimethachlone resistance levels, virulence, mycelial growth, and sensitivity to osmotic stress for the Sshk -silenced and -overexpression transformants. Overexpression of Sshk resulted in resistance to dimethachlone and increased sensitivity to various stresses and to the cell-wall-perturbing agents sodium dodecyl sulfate (SDS) and Congo red (CR). Compared with the parent isolate, Sshk -silenced transformants had reduced resistance to dimethachlone, significantly higher ( P < 0.05) mycelial growth and virulence, and lower sclerotium production, and were less sensitive to various exogenous stresses such as sodium chloride. Compared with the parent sensitive isolate HLJMG1, dimethachlone resistance ratios of the three overexpression transformants ∆C101, ∆C21, and ∆C10 increased 168.1-, 189.5-, and 221.2-fold, respectively. The three overexpression transformants were more sensitive to CR and SDS than their parent isolate. These findings suggest that overexpression of Sshk is a major mechanism for dimethachlone resistance in some isolates of S. sclerotiorum , and that Sshk plays an important role in maintaining the integrity of the cell wall. Our findings reveal a novel molecular mechanism for dimethachlone resistance in plant-pathogenic fungi.
研究领域:
植物病理学
·
分子生物学
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