JOURNAL OF FOOD PROCESSING AND PRESERVATION
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2019
·Vol. 43
·期 10
·e14116
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IF 1.288
Xiaojie Zheng,Yue He,Huan Zhou,Chunhua Xiong
This study evaluated the effects of chitosan oligosaccharides (COS)–nisin conjugates (COS-N CON) on the quality changes of Collichthys niveatus during storage at 4°C. COS–N CON were prepared by heating the aqueous solution of COS and nisin together at 80°C for 24 hr. In order to control the degree of conjugation, the total concentration and pH of mixed solution were adjusted to 2% and 2.00, respectively. COS–N CON were identified by Fourier transform infrared (FT–IR) and X–ray diffraction analysis (XRD). During storage, COS–N CON treated samples showed retarded sensory and texture deterioration, with significantly slower increase rate of pH, total viable counts (TVC), and total volatile basic nitrogen (TVB–N). At the day 6, the TVC, TVB–N, and hardness of C. niveatus treated with distilled water reached 6 log 10 (CFU/g), 28 mg/100 g, and 15 g, respectively; while that in COS–N CON treated samples was 5.1 log 10 (CFU/g), 21 mg/100 g, and 18 g, respectively. Based on indicating parameters tested, it was revealed that the COS–N CON treatment could prolong the shelf life of C. niveatus by 2 days during chilled storage, compared to the chitosan oligosaccharides (COS)–nisin mixture (COS–N MIX). Practical applications The novel preservation agent we created in this study could be considered as safe and renewable, which could be a great advantage in practical application. It was formed by conjugating chitosan oligosaccharides (COS) with nisin via Maillard reaction. Both COS and nisin were considered as green and renewable antimicrobial agents. Maillard reaction is one of the main reactions taking place in food that does not need catalyst and organic solvent. COS–nisin conjugates had a better effect on preservation of Collichthys niveatus , compared with COS–nisin mixtures, which indicating Maillard reaction could be the effective way in modifying the preservation properties of natural antimicrobial agents.
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JOURNAL OF FOOD ENGINEERING
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2026
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IF 5.8
Xiangrui Ren,Li Niu,Liping Zhao,Wenqing Zhao,Qian Lin,Shuangfeng Guo,Yayun Hu,Wenhao Li
This study focuses on the rational design of biodegradable active packaging films by combining oxidized cassava starch (OCS), polycaprolactone (PCL), and Nisin via hot-pressing. Systematic characterization demonstrated that OCS incorporation reduced PCL crystallinity through hydrogen bonding, markedly altering film microstructure and functionality, and enhancing biodegradability in synergy with Nisin. Key experimental data revealed that the ternary composite films exhibited superior comprehensive performance: the soil degradation rate reached 15.98% after 72 days, a 316% increase compared to pure PCL. Regarding antimicrobial efficacy, the inhibition rate against Staphylococcus aureus reached 85.79%. Additionally, all composite films maintained low water absorption (<2%), and some formulations exhibited excellent flexibility with a peak elongation at break of 117.98%. Molecular docking further revealed that hydrogen bonds and salt bridges stabilized the structure, with the strongest binding affinity reaching −5.0 kcal/mol, influenced by the oxidation pattern of OCS. This research presents a novel, eco-friendly ternary composite film with tunable degradation and strong antibacterial activity. This work provides a strategic framework for designing next-generation carbohydrate-based active packaging and offers a practical engineering strategy to tailor bio-based films for food preservation applications.
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Food Packaging and Shelf Life
·
2022
·
IF 8.749
Dongxiu Qi,Yewen Xiao,Li Xia,Linlin Li,Suwei Jiang,Shaotong Jiang,Hualin Wang
Colorimetric films are emerging as a facile and effective indicator for freshness monitoring. To meet the versatile demands of consumers, multifunctionality is desirable for the colorimetric films. Here, an antibacterial-antioxidant colorimetric film was developed by incorporating nisin and anthocyanins of pomegranate/ Clitoria ternatea (PGA/CTA) into ethylene vinyl alcohol (EVOH) matrix. The precursor of EVOH/nisin 10 was determined for the incorporation of PGA/CTA of 1:2. The pH-sensing nature and antioxidant activity were originated from the mixture of PGA/CTA, while the antibacterial activity was from nisin. The components of and PGA/CTA in the matrix were well-dispersed and hydrogen-bonded. The color changes of the target film of EVOH/nisin-(PGA/CTA) 3 were distinguishable at pH 2–12 and film was sensitive to the pH-stimuli of volatile ammonia and acetic acid. In addition, the film exhibited good antioxidant activity (DPPH·scavenging activity of ~80 %) and antibacterial activity against S. aureus (approximating bactericidal effectiveness). The film can not only monitor shrimp freshness effectively but extend the shelf-life of the packaged shrimp by 100 % at storage of 4 °C, revealing its potential in active intelligent food packaging. The combination of two different sourced anthocyanins along with nisin provides a strategy for achieving an efficacy of freshness retaining plus monitoring.
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FOOD CONTROL
·
2016
·
IF 3.388
Zunyang Song,Feng Li,Hui Guan,Yunfeng Xu,Quanjuan Fu,Dapeng Li
Effects of the combination of nisin and ε-polylysine with chitosan coating on quality maintenance and white blush inhibition were investigated in fresh-cut carrots. Fresh-cut carrots were treated with 1% lactic acid solution (v/v), 1% chitosan solution (w/v), or 1% chitosan solution containing 64 μ g/mL nisin and 250 μ g/mL ε-polylysine (LA + CH + Nisin + ε-PL). The samples were packed in polyethylene plastic bags and stored at 4 °C for 9 days. Changes in sensory attributes, physicochemical indices, respiration rate, microbiological counts and white blush were measured. Results showed that LA + CH + Nisin + ε-PL significantly ( P < 0.05) inhibited respiration rate, decline of ascorbic acid and growth of microorganism (yeast and mold, total viable counts, total coliforms counts, Staphylococcus aureus and Pseudomonas spp.), and increased total phenol content and phenylalanine ammonialyse (PAL) activity compared with the control after 9-day storage. It was also strongly effective in inhibiting the white blush of fresh-cut carrots. Furthermore, LA + CH + Nisin + ε-PL significantly ( P < 0.05) reduced the lignin synthesis in fresh-cut carrots by inhibiting the cinnamate-4-hydroxylase (C4H) and 4-coumarate-CoA ligase (4CL) activity, as well as Dc4CL and DcC4H gene expression. Our results may provide some basis for the use of the combination of nisin and ε-polylysine with chitosan coating as an alternative preservation method for fresh-cut carrots.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Tao Gou,Wenxiu Li,Shenglin Chen,Chao Yi,Yu Guo,Zheng Cao,Lei Zhou,KangJu Lee,Mingrui Chen,Yaowen Liu
In this study, a hydrophobic and antibacterial pad was prepared to preserve Channel Catfish ( Ictalurus punctatus ). The pad composite the microfibrillated cellulose and β-cyclodextrin/nisin microcapsules. The hydrophobic pad ensures a dry surface in contact with the fish, reducing microbial contamination. The pad has a low density and high porosity, making it lightweight and suitable for packaging applications, while also providing a large surface area for antibacterial activity. Results demonstrated that this antibacterial pad exhibits an ultralow density of 9.0 mg/cm 3 and an ultrahigh porosity of 99.10%. It can extend the shelf life of Channel Catfish fillets to 9 days at 4 °C, with a total volatile base nitrogen below 20 mg/100 g. The study proposes a novel solution for preserving aquatic products by combining antibacterial substances with the natural base material aerogel. This approach also extends the utilization of aerogel and nisin in food packaging.
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PubMed
高影响因子
Microbiology Spectrum
·
2022
·
IF 9.043
Xinxin Pang,Yansha Wu,Xiayu Liu,Yajing Wu,Qin Shu,Jianrui Niu,Qihe Chen,Xinglin Zhang
Listeria monocytogenes is a major pathogen contributing to foodborne outbreaks with high mortality. Nisin, a natural antimicrobial, has been widely used as a food preservative. However, the mechanisms of L. monocytogenes involved in nisin resistance have not yet to be fully defined. A mariner transposon library was constructed in L. monocytogenes, leading to the identification of 99 genes associated with the innate resistance to nisin via Transposon sequencing (Tn-seq) analysis. To validate the accuracy of the Tn-seq results, we constructed five mutants (ΔyqgS, ΔlafA, ΔvirR, ΔgtcA, and Δlmo1464) in L. monocytogenes. The results revealed that yqgS and lafA, the lipoteichoic acid-related genes, were essential for resistance to nisin, while the gtcA and lmo1464 mutants showed substantially enhanced nisin resistance. Densely wrinkled, collapsed surface and membrane breakdown were shown on ΔyqgS and ΔlafA mutants under nisin treatment. Deletion of yqgS and lafA altered the surface charge, and decreased the resistance to general stress conditions and cell envelope-acting antimicrobials. Furthermore, YqgS and LafA are required for biofilm formation and cell invasion of L. monocytogenes. Collectively, these results reveal novel mechanisms of nisin resistance in L. monocytogenes and may provide unique targets for the development of food-grade inhibitors for nisin-resistant foodborne pathogens.IMPORTANCE Listeria monocytogenes is an opportunistic Gram-positive pathogen responsible for listeriosis, and is widely present in a variety of foods including ready-to-eat foods, meat, and dairy products. Nisin is the only licensed lantibiotic by the FDA for use as a food-grade inhibitor in over 50 countries. A prior study suggests that L. monocytogenes are more resistant than other Gram-positive pathogens in nisin-mediated bactericidal effects. However, the mechanisms of L. monocytogenes involved in nisin resistance have not yet to be fully defined. Here, we used a mariner transposon library to identify nisin-resistance-related genes on a genome-wide scale via transposon sequencing. We found, for the first time, that YqgS and LafA (Lipoteichoic acid-related proteins) are required for resistance to nisin. Subsequently, we investigated the roles of YqgS and LafA in L. monocytogenes stress resistance, antimicrobial resistance, biofilm formation, and virulence in mammalian cells.
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PubMed
高影响因子
FOOD CONTROL
·
2021
·
IF 5.548
Fangting Bai,Du Guo,Yueyi Wang,Shanbo Zhang,Jiahui Li,Kangkang Zhi,Chao Shi,Xiaodong Xia
In this study, the combined bactericidal effect of nisin and thymoquinone (TQ) on Listeria monocytogenes was investigated in Tryptone Soy Broth (TSB) and sterilized milk. The combined treatment resulted in a greater reduction in bacterial number than either nisin or TQ treatment alone. Nisin and TQ respectively caused depolarization and hyperpolarization of the cell membrane of L. monocytogenes , which indicated early injury to the bacteria. Confocal laser scanning microscopy and N -phenyl-l-naphthylamine uptake assay showed disruption of the cell membrane on treatment. Field emission scanning electron microscopy revealed depression and collapse of the cell surface following treatment with nisin and TQ. Growth curves showed that nisin and TQ acted on the bacteria in different growth phases. Fourier-transform infrared spectroscopy demonstrated a reversible hydrogen-bonding interaction between nisin and TQ. Our results suggest that nisin combined with TQ is a more effective antibacterial effect against L. monocytogenes in both TSB and in sterilized milk than either agent alone. Our study sheds light on the inhibitory mechanisms of nisin, TQ, and their combination, and provides a theoretical basis for the synergistic antibacterial application of nisin and TQ in the food industry.
研究领域:
食品微生物学
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抗菌剂
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食品安全
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应用微生物学
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高影响因子
Journal of Future Foods
·
2026
·
IF 7.2
Bin Ma,Xianmin Qin,Huiying He,Xinyao Cheng,Keshan Wang,Yongguo Jin,Yuanyuan Liu
To enhance the application effectiveness of edible polymeric materials in practical coating-based preservation. Here, an egg white/chitosan-based biocomposite coatings modified by the addition of tannic acid and nisin was prepared based on previous research, and the effects of two additives on the drying rate and adhesion of film-forming solution during the actual coating process were investigated. The prepared egg white-chitosan-tannic acid-nisin composite coating (EW40/TNCS60) exhibited a 18.2% increase in moisture diffusivity compared to the egg white-chitosan coating (EW40/CS60), accelerating the curing process. The EW40/TNCS60 coating also displayed more favorable apparent viscosity, which would provide superior adhesion for meat storage. Furthermore, the water contact angles on pig skin, fatty meat, and lean meat were all less than 90°, confirming excellent wettability. Surface tension and flowability analysis further revealed that the EW40/TNCS60 coating demonstrated desirable the interfacial adsorption behavior on pork surfaces. Thus, this work offers a feasible strategy for developing coatings with fast drying rate and high adhesion to achieve meat preservation.
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2026
·
IF 7.1
Junjie Wu,Zongyang Li,Yu Zhang,Jiahang Wei,Yougui Zhang,Wenlong Yu,Yaxin Sang,Xianghong Wang
A novel HACC/lignin composite film incorporating nisin was successfully fabricated. • Lignin incorporation endows the film with superior ultraviolet-blocking capability. • The addition of nisin significantly improves the mechanical and barrier properties. • Nisin confers potent and broad-spectrum antibacterial activity to the composite film. • The film effectively extends grape shelf life by antibacterial and anti-UV functions. A multifunctional composite packaging film integrating chitosan quaternary ammonium salt (HACC), lignin, and nisin was developed to achieve synergistic UV-blocking and antibacterial performance. Nisin, uniformly dispersed throughout the HACC/lignin matrix via hydrogen bonding, not only imparted potent antibacterial activity but also reinforced mechanical strength, thermal stability, and barrier performance. Of all formulations evaluated, HLN-0.4 delivered the most favorable combination of properties: a tensile strength of 5.99 MPa, an elongation at break of 141.21%, and effective antibacterial activity against Staphylococcus aureus , Bacillus subtilis , and Listeria monocytogenes . In grape preservation trials, HLN-0.4 extended postharvest shelf life to 15 days, curtailed weight loss from 17.9% to 9.0%, and sustained fruit firmness at 29.74 N, approximately 63% above the 18.28 N measured in unpackaged controls. Taken together, these findings position HLN-0.4 as an effective, sustainable, and scalable packaging solution with strong potential for extending postharvest shelf life and maintaining the quality of fresh produce. Download: Download high-res image (306KB) Download: Download full-size image
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Polymers
·
2022
·Vol. 14
·期 23
·5159
·
IF 4.967
Yiqi Chen,Yujie Duan,Han Zhao,Kelan Liu,Yiqing Liu,Min Wu,Peng Lu
Bio-based porous materials can reduce energy consumption and environmental impact, and they have a possible application as packaging materials. In this study, a bio-based porous foam was prepared by using a Pickering emulsion as a template. Nisin and waterborne polyurethane (WPU) were used for physical modification of 2,2,6,6-tetramethyl piperidine-1-oxyl-oxidized cellulose nanocrystals (TOCNC). The obtained composite particles were applied as stabilizers for acrylated epoxidized soybean oil (AESO) Pickering emulsion. The stability of the emulsion was characterized by determination of the rheological properties and microscopic morphology of the emulsion. The emulsion stabilized by composite particles showed better stability compared to case when TOCNC were used. The porous foam was obtained by heating a composite-particles-stabilized Pickering emulsion at 90 °C for 2 h. SEM (scanning electron microscopy) images showed that the prepared foam had uniformly distributed pores. In addition, the thermal conductivity of the foam was 0.33 W/m·k, which was a significant decrease compared to the 3.92 W/m·k of the TOCNC foam. The introduction of nisin and WPU can reduce the thermal conductivity of the foam, and the physically modified, TOCNC-stabilized Pickering emulsion provides an effective means to preparing bio-based porous materials.Keywords:TOCNC/Nisin/WPU;Pickering emulsion;biomaterials;porous foam;thermal insulation
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PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Lixin Kang,Qiufang Liang,Huanxin Chen,Qiusuo Zhou,Zhuzhong Chi,Arif Rashid,Haile Ma,Xiaofeng Ren
This study developed two novel food packaging films, oat protein/pullulan (Op/Pul) and Nisin-loaded oat protein/pullulan (Nis@Op/Pul) films. Ultrasound was introduced to improve its mechanical, structural and physicochemical properties. The Op/Pul film has lower light transmittance, water vapour and oxygen permeability (OP) and improved film uniformity than pure oat protein and pullulan film. The addition of Nisin led to a significant decrease in the composite films' transparency, moisture content, and total soluble matter (TSM). The ultrasound treatment significantly increased the elongation at break and transparency of Nis@Op/Pul film by 18.37% and 8.03% and decreased its TSM and OP by 8.33% and 2.78%, respectively, compared to the conventional method. The structure analysis shows ultrasound enhances intermolecular hydrogen bonding, reduces the crystallinity and formed a more regular, uniform surface. Moreover, the Nis@Op/Pul film prepared by ultrasound treatment could effectively delay the decay and deterioration of fresh strawberries and prolong their shelf life.
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高影响因子
Frontiers in Microbiology
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2016
·
IF 4.165
Rao Lei,Wang Yongtao,Chen Fang,Liao Xiaojun
The inactivation effects of high pressure CO2 + nisin (simultaneous treatment of HPCD and nisin, HPCD + nisin), HPCD→nisin (HPCD was followed by nisin), and nisin→HPCD (nisin was followed by HPCD) treatments on Bacillus subtilis spores in aqueous solutions were compared. The spores were treated by HPCD at 6.5 or 20 MPa, 84–86°C and 0–30 min, and the concentration of nisin was 0.02%. Treated spores were examined for the viability, the permeability of inner membrane (IM) using flow cytometry method and pyridine-2, 6-dicarboxylic acid (DPA) release, and structural damage by transmission electron microscopy. A synergistic effect of HPCD + nisin treatment on inactivation of the spores was found, and the inactivation efficiency of the spores was HPCD + nisin > HPCD→nisin or nisin→HPCD. Moreover, HPCD + nisin caused higher IM permeability and DPA release of the spores than HPCD. A possible action mode of nisin-enhanced inactivation of the spores was suggested as that HPCD firstly damaged the coat and cortex of spores, and nisin penetrated into and acted on the IM of spores, which increased the damage to the IM of spores, and resulted in higher inactivation of the spores.
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