JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2012
·Vol. 12
·期 11
·8266-8275
·
IF 1.563
Wang Yang,Chang Baisong,Yang Wuli
In this work, pH-sensitive nanoparticles based on polyketal copolymer (PK3) were formulated by encapsulating hydrophilic doxorubicin hydrochloride (DOX) and hydrophobic paclitaxel (PTX) into the polymer, respectively. Polyketal PK3 was synthesized by the acetal exchange reaction between
2,2-dimethoxypropane and diols. DOX-loaded nanoparticles were fabricated through a modified double emulsion method and PTX-loaded nanoparticles were obtained by single emulsion method. The results demonstrated that the drug loading content increased with the increase of drug/polymer mass ratio,
and PTX was entrapped into PK3 nanoparticles more effectively due to its hydrophobic character and the single emulsion method. The in vitro release behaviors of the DOX-loaded and PTX-loaded nanoparticles exhibited that both DOX and PTX release were pH-dependent, and the release rate
was much faster in acid environment than that in neutral environment. The cytotoxicity assay of blank nanoparticles indicated that polyketal copolymer (PK3) was biocompatible and suitable as drug vehicle, while PK3 nanoparticle encapsulating DOX or PTX could kill the cancer cells sufficiently
and efficiently. Thus, the pH-sensitive PK3 nanoparticle is a promising carrier for both hydrophilic and hydrophobic anticancer drugs.
研究领域:
药剂学
·
生物医学工程
·
肿瘤学
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DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2014
·Vol. 50
·期 6
·2429-2442
·
IF 2.305
Zhang Han,Xue Yanan,Huang Juan,Xia Xiaoyang,Song Meifang,Wen Kaikai,Zhang Xiaoxiao,Luo Xiaogang,Cai Ning,Long Sihui,Yu Faquan
A charge reversible, magnetic-targeted, and pH-responsive nano therapy system assembled from citraconic-modified dextran (Dex–COOH), glycine-stabilized superparamagnetic iron oxide nanoparticles (SPION–NH 2 ), and doxorubicin (DOX) through electrostatic interaction was designed for delivering therapeutics to tumor cells. Dex–COOH was first synthesized to build the pH-sensitive citraconic amide bond and further render charge reversal upon the cleavage of the linkage. The SPION–NH 2 anchored in the nanocarrier endows the carrier with magnetic migration. The vibrating sample magnetometer analysis exhibits the superparamagnetic behavior, and the magnetic saturation value is estimated to be 39.6 emu (g Fe) −1 . The change of size and the reversal of zeta potential from negative to positive in acidic environment confirmed these expected responsibilities. The DOX-loaded nanocarriers contain DOX up to 13.0 wt%. An in vitro release profile demonstrated an efficient DOX release of 80 % at pH 5.0 while of 27 % at pH 6.8 and 13 % at pH 7.4, suggesting a pH-induced release mechanism. Microscopic images of Prussian blue staining, quantification of cellular iron and protein concentration displayed apparent iron uptake by HeLa cells. Confocal laser scanning microscopy observations revealed that the nanocarriers could efficiently deliver and release DOX into the nuclei of HeLa cells. MTT assays testified that DOX-loaded nanocarrier exhibited high anti-tumor activity with IC 50 of 0.7 μg mL −1 , while plain nanocarriers were practically non-toxic. This drug delivery system has shown the ability to improve the chemotherapeutic efficacy and to reduce the side effects, indicating great potential for delivery of drugs to the targeted sites in patients.
研究领域:
药剂学
·
生物医学工程
·
肿瘤学
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DOI
高影响因子
JOURNAL OF LIGHTWAVE TECHNOLOGY
·
2024
·
IF 4.1
Guosheng Zhang,Zhiyu Wang,Jinhui Shi,Sheng Zhou,Benli Yu
Overcoming the impact of pH on the performance of random lasers (RLs) has emerged as a significant challenge in its application for biomedical therapy. To tackle this issue, this paper proposes a pH-insensitive hydrogel fiber RLs. The RLs consists of Rhodamine B dye-doped p(acrylamide-co-acrylic acid, AM-co-AA) hydrogel as the fiber core and calcium alginate (Ca-alginate) hydrogel as the cladding, termed as p(AM-co-AA)/Ca-alginate hydrogel fiber RLs. The prepared RLs not only operate reliably at different pH levels but also allow for tuning of its emission wavelength by adjusting the temperature, ensuring the reliability and safety of photothermal therapy. Experimental results demonstrate that the threshold of p(AM-co-AA)/Ca-alginate hydrogel fiber RLs is only 0.23 mJ/cm². Moreover, within the range of 20∼80°C, the random laser emission wavelength can be tuned by controlling the temperature, with a maximum tunable range of 5.6 nm and good reversibility. Most importantly, the random laser intensity and wavelength of the p(AM-co-AA)/Ca-alginate hydrogel fiber RLs exhibit excellent stability when the pH value changes within the range of 3.5 to 9.5, indicating its exceptional ability to resist pH interference. We believe that the hydrogel fiber RLs proposed in this paper, leveraging the porous structure of hydrogel to reduce the laser threshold and employing the “core-cladding” structure to enhance stability, holds broad potential applications in the biomedical field.
研究领域:
生物医学工程
·
材料科学
·
光学
✓ 已验证
产品关联
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2020
·
IF 5.88
Shengmei Song,Junhui Hu,Minglu Li,Xiaojuan Gong,Chuan Dong,Shaomin Shuang
The fluorescent boron, nitrogen and sulfur co-doped carbon dots (BNSCDs) were prepared by simple hydrothermal reaction of 4-carboxyphenylboronic acid and 2,5-diaminobenzenesulfonic acid at 200 °C for 8 h. The fluorescence of the BNSCDs could be quenched by Fe 3+ based on the electron transfer between Fe 3+ and BNSCDs, so a label-free, good selectivity and high sensitivity method for Fe 3+ determination was established with linear range and LOD of 1.5–692 μmol/L and 87 nmol/L, respectively. And then the fluorescent probe was employed for detection of Fe 3+ in tap water, coal gangue, fly ash and food samples successfully. Moreover, the as-prepared BNSCDs could serve as a novel pH fluorescent probe in the range of pH 1.60–7.00, which could be attributed to the proton transfer of carboxyl groups on the surface of BNSCDs. More importantly, the pH fluorescent probe possesses fast, real-time and low toxicity, applying for intracellular pH fluorescence imaging in HIC, HIEC, LO2 and SMMC7721 cells. In view of its simplicity, timely response and outstanding compatibility, the as-fabricated BNSCDs show the potential applications in water quality and solid waste monitoring, food detection, real-time measuring of intracellular pH change in vitro.
研究领域:
分析化学
·
生物医学工程
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
LANGMUIR
·
2013
·
IF 4.187
Fei Qu,Nian Bing Li,Hong Qun Luo
Silver nanoclusters capped by hyperbranched polyethylenimine (PEI) have been developed as a highly sensitive fluorescent and colorimetric pH sensor. The probe responds rapidly to pH fluctuations and has such absorption characteristics that the color changes from the colorless or a nearly colorless state to a colored state with increasing acidity, so PEI-capped Ag nanoclusters could be used as a color indicator for colorimetric pH detection. Quantitatively, the fluorescence intensity of PEI-capped Ag nanoclusters exhibits a linear fashion over the pH range of 5.02–7.96 and increases by around 10-fold approximately with greater fluorescence at higher pH values. The repulsion development and conformational change of PEI with decreasing pH induce the aggregation of Ag nanoclusters, leading to an obvious color change and fluorescence quenching of Ag nanoclusters at low pH values. As expected, the pH probe is also sensitive to the different buffer solutions, except for those containing some anions that could react with Ag nanoclusters. Besides, the ionic strength of the buffers has a little influence on the pH-responsive behavior. Our pH sensor with nanoscaled physical dimensions would be a promising candidate in the applications in biological, medical, and pharmaceutical fields.
研究领域:
纳米技术
·
分析化学
·
生物医学工程
·
材料科学
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2015
·Vol. 39
·期 6
·4568-4574
·
IF 3.086
Shuchao Hu,Ling Sun,Mingxing Liu,Hongda Zhu,Huiling Guo,Hongmei Sun,Honghao Sun
For accurate determination of local intracellular pH which could further improve our understanding of cellular processes and knowledge of advanced drug delivery system architecture, a fluorescent-based ratiometric nanosensor has been designed over the past decade. As a promising matrix for nanosensors and nanomedicines, silica nanoparticles (SNPs) have been widely used due to their desirable properties, which include a high loading level of guest molecules, low toxicity and easy functionalization. Amination of SNPs was essential for binding functional groups, such as fluorescent molecules and targeting groups, whereas positively charged amine will cause the aggregation of SNPs which limits the further application of SNPs. To circumvent this problem, a simple and effective strategy has been presented in this paper. Firstly, an acid was used as a catalyst instead of a base to inhibit SNP aggregation in the amination process. Subsequently, polymers (poly(ethylene glycol) and hyaluronic acid) were coated on the surface of monodispersed aminated SNPs to obtain excellent colloidal stability. The well dispersed core–shell SNPs were further functionalized with two pH sensitive fluorophores (fluorescein isothiocyanate and Oregon Green isothiocyanate) and one reference fluorophore (rhodamine B isothiocyanate), which resulted in ratiometric pH nanosensors with a hydrodynamic diameter of 76–100 nm. The sensors exhibited a broad pH measurement range from 3.8 to 7.4, which covers almost all intracellular pH values, and remarkable colloidal stability in buffer solution.
研究领域:
纳米技术
·
生物传感器
·
材料科学
·
药物传递系统
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Yaosheng Liu,Yongxing Diao,Guangxing Hu,Yuyan Zhao,Yan Shi,Hongda Wang,Zhuang Li
Metal-based pH sensor has wide applications in industrial monitoring, soil environment, marine safety, and other fields. In this study, we proposed to renew the antimony-based sensor by physical polishing and electrochemical cathode reduction. Compared with before polishing, the pH response sensitivity after polishing increased from 0.034 V/pH to 0.042 V/pH, electrochemical cathode reduction renewed the pH response sensitivity improved from 0.018 V/pH to 0.032 V/pH (10 min) and 0.043 V/pH (30 min). The reasons for the deterioration of the pH response performance of the antimony-based sensor were analyzed by SEM, EDS, and XRD, and the renewable mechanisms of the sensor were proposed. The renewable effect of electrochemical cathode reduction on the degradation of sensor accuracy was evaluated for long-term monitoring. It is expected to realize remote online renewal of sensors under on-site unattended conditions, to reduce operation and maintenance costs of the sensor. These results provide solutions for the repeated regeneration of metal-based pH sensors which were degraded in performance and have significance for promoting the development and wide applications of renewable pH sensors.
研究领域:
材料科学
·
环境监测
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2017
·Vol. 24
·期 1
·212-221
·
IF 5.317
Jun Song,Yanlong Wei,Jiwen Hu,Guojun Liu,Zhengzhu Huang,Shudong Lin,Feng Liu,Yangmiao Mo,Yuanyuan Tu,Ming Ou
Graphical Gated delivery : pH-Responsive porous nanocapsules have been prepared from ternary graft copolymers and used as potential vehicles for controlled release of drugs, food, and bioactive compounds (see figure). In this work pH-responsive porous nanocapsules have been successfully prepared from a ternary graft copolymer, poly(glycidyl methacrylate)- g -[poly(2-cinnamoyloxyethyl methacrylate)- r -poly(ethylene glycol) methyl ether- r -poly(2-diethylaminoethyl methacrylate)] or PGMA- g -(PCEMA- r -MPEG- r -PDEAEMA). The graft copolymers were fabricated by grafting three types of polymer chains onto the backbone polymer by using click chemistry. These ternary copolymers underwent self-assembly to form vesicles in a DMF/water solvent mixture. While the MPEG chains served as the corona and stabilized the vesicles, the vesicle wall was composed of a dominant PCEMA continuous phase that was interspersed by PDEAEMA domains. After photo-cross-linking, the PDEAEMA domains were embedded in the structurally locked PCEMA wall. By decreasing the pH of the external solution, we were able to trigger the release of encapsulated pyrene due to the capsule wall becoming porous as a result of the PDEAEMA chains bearing positively charged amine groups stretching into the water. While these pH-responsive porous nanocapsules exhibited potential applications in drug delivery, detection and catalysis, the strategy reported in this contribution also represented a new paradigm for the design and preparation of other novel stimuli-responsive porous nanocapsules.
研究领域:
材料科学
·
药学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2025
·Vol. 116
·期 1
·10
·
IF 2.2
Huang Junyun,Liu Boying,Yuan Sijia,Zhou Haiyun,Bao Jian,Tu Yong,Wang Zunyao,Wang Weixia
The UV filter benzophenone-3 (BP-3) is a prevalent environmental pollutant due to its extensive use in personal care products, raising concerns about its ecological risks. This study investigated the pH-dependent oxidative stress effects of benzophenone-3 (BP-3) on Carassius auratus by measuring four key biomarkers—superoxide dismutase, catalase, glutathione, and malonaldehyde in liver tissue. The results demonstrated that BP-3 exposure led to significant oxidative stress, as evidenced by altered antioxidant levels and increased lipid peroxidation. The integrated biomarker response indicated that stress was more pronounced at higher BP-3 concentrations and varied with pH, being greatest under acidic conditions. These findings demonstrate that the toxicity of BP-3 is strongly influenced by environmental pH, highlighting the necessity of incorporating pH variations into ecological risk assessments of ionizable organic pollutants.
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DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Jiaqing He,Qiang Zhou,Zhiqing Ge,Shunfeng Jiang,Jiahe Li,Wei Feng,Haiyang Yang
Temperature-responsive hydrogels including lower critical solution temperature (LCST)- and upper critical solution temperature (UCST)-type hydrogels are attractive in various fields. However, the swift switch between LCST and UCST stimuli-responsive behaviors remains intriguing and challenging. Here is reported a kind of hydrogel with pH-gated LCST and UCST response behaviors. This is achieved using the hydrogen bonding between carboxylic acid groups of poly(acrylic acid- co -acrylamide) and hydroxyl groups of hydroxypropyl cellulose (HPC). The poly(acrylic acid- co -acrylamide)-HPC (PACA-HPC) hydrogels exhibit pH-gated reversible LCST-UCST phase transition behavior. When the transparent PACA-HPC hydrogel is placed in an acid bath, the COO − groups get protonated, rapidly forming hydrogen bonds with HPC to render a light-scattering state making the hydrogel opaque. Furthermore, the opaque hydrogel exhibits UCST phase transition behavior at 20–45 °C. When the opaque PCAC-HPC hydrogel is placed in an alkaline environment, hydrogen-bonded complexes gradually dissociate as the COOH groups are deprotonated to form a homogeneous transparent state. The transparent hydrogel exhibits LCST phase transition behavior at 20–45° C. Therefore, is shown the hydrogen bonding strategy to fabricate hydrogels with tunable LCST and UCST responses. With this pH-gated hydrogel with switchable LCST/UCST responsive behaviors, are demonstrated its applications in smart windows and information encryption.
研究领域:
材料科学
·
化学工程
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Haihua Jiang,Junhui Pan,Xing Hu,Miao Zhu,Deming Gong,Guowen Zhang
Glutenin has limited applicability in food industry due to poor water solubility and emulsifying properties. In this study, the physicochemical properties of glutenin were improved by combined treatments of alkaline pH-shifting or acidic pH with heating. The surface morphology, structure and physicochemical properties were measured during the modification process of glutenin. Results showed that the smaller square clusters and regular tubular fibrils were observed in modified glutenin and the α-helix proportion of the treated glutenin was finally increased to 59.90 ± 0.01%. Compared with untreated glutenin, the combined treatments of pH-shifting with heating as well as fibrillation process increased the solubility of glutenin by 21.3 and 3.5 times, respectively. Moreover, the treated glutenin showed excellent emulsifying stability (EAI: 50.84 ± 0.51 m 2 g −1 ) and thermal stability (peak temperature increased from 109.58 to 149.05 °C). This study provides an informative basis for improving the physicochemical and functional properties of glutenin.
研究领域:
食品科学
·
蛋白质化学
✓ 已验证
DOI
PubMed
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2025
·Vol. 116
·期 1
·9
·
IF 2.2
Huang Junyun,Liu Boying,Yuan Sijia,Zhou Haiyun,Bao Jian,Tu Yong,Wang Zunyao,Wang Weixia
Benzophenone-3 (BP-3), a widely used ultraviolet filter in personal care products, has been extensively detected in aquatic environments and poses potential ecological risks. This study innovatively investigated the pH-dependent acute toxicity of BP-3 to three model aquatic organisms— Daphnia magna , Photobacterium phosphoreum , and Danio rerio —by determining the EC 50 and LC 50 values across a pH range of 5.0 to 9.0. Our results revealed that BP-3 exerted greater acute toxicity on all three tested aquatic organisms under acidic conditions (pH 5.0) than under alkaline conditions (pH 9.0). The increased toxicity with lower pH was attributed to the predominance of the more lipophilic molecular form of BP-3, emphasizing the significant influence of environmental pH on BP-3 toxicity. These findings provide essential toxicological data for assessing the ecological risks of BP-3 in diverse aquatic environments, highlighting the necessity of considering BP-3 concentration, environmental pH and exposed species in ecological risk assessments.
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PubMed
高影响因子
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