TALANTA
·
2024
·
IF 5.6
Sheng Gong,Lingxuan Ma,Fan Nie,Mai Luo,Sijia Wu,Ting Wang,Yu Yang,Di Chen,Jinchao Wei,Peng Li
Unreasonable or illegal utilization of pesticides may lead to pollution of agricultural products, especially with some persistent but effective pesticides. Hence, there is an urgent need to develop sensitive and rapid methods for pesticide detection to ensure the safety of agricultural products. Herein, a dual-mode ratiometric sensing system utilizing two gold nanoclusters (G/R–AuNCs) was designed and constructed for paraquat (PQ) detection, a typical, highly toxic, widely used pesticide. Initially, a ratiometric fluorescent method was developed based on green-emitting (Em∼530 nm) and red-emitting (Em∼655 nm) gold nanoclusters. Relying on the electrostatic interaction between G-AuNCs and PQ, G-AuNCs were quenched as a sensing unit. R–AuNCs with chemical inertness and excellent stability to PQ served as correction signals. The selective and sensitive detection of PQ was successfully achieved with a satisfactory linear relationship within the range of 5–500 μg/L, and the limit of detection (LOD) was as low as 1.68 μg/L (6.5 nM). Furthermore, PQ can be detected through visual analysis based on smart mobile devices. As the concentration increased, a more noticeable color change occurred, producing a significant shift from yellow to red under 365 nm UV light, resulting in a striking visual effect. This research not only showcases the practical benefits of the dual-mode analytical method but also drives forward the application of ratiometric probes.
研究领域:
分析化学
·
环境科学
·
纳米生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Yina Yang,Wenxuan Zhu,Huijun Liu,Jiaying Wang,Xin Jia,Yuchun Tao,Jing Meng,Qingyu Meng,Jing Dai,Lijia Yu,Chunguang Ding
Paraquat (PQ), a highly toxic and persistent herbicide, poses severe risks to environmental and public health. Herein, we developed a supramolecular indicator displacement assay (IDA) for PQ detection using a squaraine dye (J3-Ad) and cucurbit[7]uril (CB7). PQ displaces J3-Ad monomers from the J3-Ad&CB7 (J3&CB) complex, inducing J3-Ad self-assembly into H-aggregates. This process was characterized by Ultraviolet–visible (UV–vis) absorption and fluorescence spectroscopy, proton nuclear magnetic resonance ( 1 H NMR) spectroscopy, and density functional theory (DFT) calculations. The assay achieved a detection limit of 1.86 μM with a linear range of 0–30 μM, and exhibited excellent anti-interference ability. It was successfully applied to visually detect PQ in soil and fruit samples, with recovery rates of 96.21–104.66%. This method provides a simple, rapid, and reliable approach for monitoring PQ residues in environmental and food safety applications.
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2020
·
IF 3.721
Yujie Zhang,Yunlong Huang,Ling Fu,Jiaoyan Qiu,Zhuqing Wang,Aiguo Wu
Paraquat (PQ) is a kind of herbicide, which is highly toxic to human body. In this work, we proposed a fast and simple detection method for PQ in aqueous samples based on electrostatic interactions between modified gold nanoparticles (Au NPs) and PQ. Au NPs were first modified with sodium 3-mercapto-1-propanesulfonate (Au NPs-3MPS). 3MPS with negative charge can interact with nitrogen atoms of PQ with positive charge via electrostatic force. As a result, Au NPs-3MPS aggregated together. Accordingly, the color of Au NPs-3MPS colloid changed from red to blue-gray, and the UV–vis absorption spectrum changed accordingly. The detection limit of PQ is 0.1 mg/L by naked eyes, and 0.001 mg/L by UV–vis spectroscopy. Moreover, this detection method has good specific selectivity and anti-interference performance, and it has been successfully used for detecting PQ in real environmental samples and fruit juice samples.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
高影响因子
Oxidative Medicine and Cellular Longevity
·
2021
·
IF 6.543
Zhen-Dong Zhang,Ya-Jun Yang,Zhe Qin,Xi-Wang Liu,Shi-Hong Li,Li-Xia Bai,Jian-Yong Li
Aspirin eugenol ester (AEE) is a new pharmaceutical compound esterified by aspirin and eugenol, which has anti-inflammatory, antioxidant, and other pharmacological activities. The aim of this study was to investigate the protective effect of AEE on paraquat- (PQ-) induced cell damage of SH-SY5Y human neuroblastoma cells and its potential molecular mechanism. There was no significant change in cell viability when AEE was used alone. PQ treatment reduced cell viability in a concentration-dependent manner. However, AEE reduced the PQ-induced loss of cell viability. Flow cytometry, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), and 46-diamidino-2-phenylindole (DAPI) staining were used to evaluate cell apoptosis. Compared with the PQ group, AEE pretreatment could significantly inhibit PQ-induced cell damage. AEE pretreatment could reduce the cell damage of SH-SY5Y cells induced by PQ via reducing superoxide anion, intracellular reactive oxygen species (ROS), and mitochondrial ROS (mtROS) and increasing the levels of mitochondrial membrane potential (). At the same time, AEE could increase the activity of glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) and decrease the activity of malondialdehyde (MDA). The results showed that compared with the control group, the expression of p-PI3K, p-Akt, and Bcl-2 was significantly decreased, while the expression of caspase-3 and Bax was significantly increased in the PQ group. In the AEE group, AEE pretreatment could upregulate the expression of p-PI3K, p-Akt, and Bcl-2 and downregulate the expression of caspase-3 and Bax in SH-SY5Y cells. PI3K inhibitor LY294002 and the silencing of PI3K by shRNA could weaken the protective effect of AEE on PQ-induced SH-SY5Y cells. Therefore, AEE has a protective effect on PQ-induced SH-SY5Y cells by regulating the PI3K/Akt signal pathway to inhibit oxidative stress.
研究领域:
药理学
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毒理学
·
细胞生物学
·
神经科学
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产品关联
DOI
PubMed
高影响因子
BIOMEDICAL CHROMATOGRAPHY
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2015
·Vol. 30
·期 1
·75-80
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IF 1.723
Congcong Wen,Zhiyi Wang,Meiling Zhang,Shuanghu Wang,Peiwu Geng,Fa Sun,Mengchun Chen,Guanyang Lin,Lufeng Hu,Jianshe Ma,Xianqin Wang
Paraquat is quick-acting and non-selective, killing green plant tissue on contact; it is also toxic to human beings and animals. In this study, we developed a urine metabonomic method by gas chromatography–mass spectrometry to evaluate the effect of acute paraquat poisoning on rats. Pattern recognition analysis, including both partial least squares discriminate analysis and principal component analysis revealed that acute paraquat poisoning induced metabolic perturbations. Compared with the control group, the levels of benzeneacetic acid and hexadecanoic acid of the acute paraquat poisoning group (intragastric administration 36 mg/kg) increased, while the levels of butanedioic acid, pentanedioic acid, altronic acid decreased. Based on these urinary metabolomics data, support vector machine was applied to discriminate the metabolomic change of paraquat groups from the control group, which achieved 100% classification accuracy. In conclusion, metabonomic method combined with support vector machine can be used as a useful diagnostic tool in paraquat-poisoned rats. Copyright © 2015 John Wiley & Sons, Ltd.
研究领域:
毒理学
·
分析化学
·
生物信息学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Mengqing Cheng,Jing Zhang,Ting Huang,Longshua Qin,Huanhuan Dong,Fusheng Liao,Hao Fan
This study presents functionalized metal–organic frameworks nanosheets (RuMOFNSs) with strong electrochemiluminescence (ECL) and fluorescence (FL) properties and a novel signal marker-tetraferrocene. Based on the efficient quenching effect of the tetraferrocene on RuMOFNSs, a “signal switch” ECL-FL dual-mode sensor is constructed for sensitive detection of paraquat (PQ). ECL and FL signals are annihilated after adding paraquat-aptamer DNA (PQ-Apt DNA) labeled with tetraferrocene since it is close to RuMOFNSs. PQ is added, and the strong binding and intermolecular interaction between PQ-Apt DNA and PQ induces spatial separation, with tetraferrocene groups far away from RuMOFNSs. At this point, ECL and FL signals are restored. The change in ECL and FL signals realized the quantitative determination of the PQ solution. In addition, the dual-mode sensor exhibits high sensitivity and specificity with detection limits as low as 0.008 ng/mL and 0.059 ng/mL. The proposed sensor is successfully applied to determine PQ, indicating its great application potential in the food industry.
研究领域:
分析化学
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环境科学
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材料科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Shengchen Lv,Donghui Liu,Xueke Liu,Zhiqiang Zhou,Peng Wang
A novel fluorescent sensor utilizing customized carbon dots (CDs) has been developed for the rapid and efficient detection of the herbicide paraquat. The 4,4′-diamino-2,2′-stilbenedisulfonic acid (DSD acid) functionalized CDs (DSD-CDs) were synthesized by a hydrothermal method using citric acid and L-cysteine as precursors, followed by amidation with DSD acid. The resultant DSD-CDs exhibited bright blue fluorescence upon UV excitation. The fluorescence of the functionalized carbon dots was effectively quenched by paraquat, following a static quenching mechanism as confirmed by fluorescence lifetime measurements. Interestingly, the mechanism study revealed that the excited-state of the DSD-CDs could interact effectively with paraquat, resulting in stronger fluorescence quenching compared to the ground-state. The sensor showed high sensitivity, selectivity, and stability, with a wide linear range of 0.01 ∼ 2 mg/L (R 2 = 0.997) and a low detection limit of 0.003 mg/L. The developed fluorescent sensor demonstrated exceptional potential for paraquat detection in actual samples, encompassing blood, urine, soybean, and river water, with recovery rates exceeding 83 % and relative standard deviations (RSD) below 10 %. This underscores its practical utility in environmental protection and clinical testing applications.
研究领域:
分析化学
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环境科学
·
生物医学
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DOI
高影响因子
TOXICOLOGY
·
2021
·
IF 4.221
Zhen-Dong Zhang,Ya-Jun Yang,Xi-Wang Liu,Zhe Qin,Shi-Hong Li,Jian-Yong Li
Paraquat (PQ) is an effective and commercially important herbicide that is widely used worldwide. However, PQ is highly toxic and can cause various complications and acute organ damage. Aspirin eugenol ester (AEE) is a potential new compound with anti-inflammatory and antioxidant stress pharmacological activity. The present study was to reveal the therapeutic effects and the protective effect of AEE against PQ-induced acute lung injury (ALI) with the help of PQ-induced oxidative damage in A549 cells and PQ-induced lung injury in rats. AEE might have no significant therapeutic effect on PQ-induced lung injury in rats. However, AEE had a significant protective effect on PQ-induced lung injury in rats. AEE pretreatment significantly reduced the stimulatory effect of PQ on malondialdehyde (MDA), the inhibitory effect of PQ on catalase (CAT) activity, superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity, the ratio of GSH/GSSH, the activity of caspase-3 and the overexpression of p38 mitogen-activated protein kinase (MAPK) phosphorylation in vivo . In vitro , A549 cells were treated with 250 μM PQ for 24 h. Incubation of A549 cells with PQ led to apoptosis, and increased the level of superoxide anions, reactive oxygen species (ROS), malondialdehyde and the activity of caspase-3 and up-regulation of phosphorylated p38-MAPK, reduced mitochondrial membrane potential (ΔΨm) and the activity of SOD. However, after 24 h on AEE pretreatment of A549 cells, the above-mentioned adverse reactions caused by PQ were significantly alleviated. In addition, AEE pretreatment reduced p38-MAPK phosphorylation in PQ-treated A549 cells. SB203580, the specific p38-MAPK inhibitor, and p38-MAPK shRNA attenuated the activation of the p38-MAPK signaling pathway. N-acetylcysteine (NAC) reduced the level of phosphorylated p38-MAPK and the production of intracellular ROS and inhibited apoptosis. The results showed that AEE may inhibit PQ-induced cell damage through ROS/p38-MAPK-mediated mitochondrial apoptosis pathway.
研究领域:
药理学
·
毒理学
·
细胞生物学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Mingchun Lv,Nisar Hussain,Da-Wen Sun,Hongbin Pu
An optimum SERS performance is mainly dependent on the development of innovative substrates that show high spectral reproducibility and stability. The current study involves the synthesis of core–shell nanorods (Au@AgNRs) functionalized with mercaptoacetic acid (MAA) to increase the stability and sensitivity of the substrate for cost-effective and sensitive assessment of paraquat (PQ) residues in fruit samples. The morphological analysis of the substrate using TEM and STEM-EDS images indicated the uniformity in size and structure of the prepared nanoparticles. Further the modified substrate, MAA/Au@AgNRs showed excellent stability and sensitivity in comparison with its unmodified counterpart. For the evaluation of PQ residues in pear and apple fruit samples the modified substrate exhibited R 2 values of 0.9954 and 0.9914 with a low limit of detections of 0.012 and 0.024 ppm, and good percent recoveries of 80–110 % and 81–104 %, respectively. Thus, the study proved the effectiveness of the proposed MAA/Au@AgNRs substrate for assessing residues of herbicide in complex foods.
研究领域:
分析化学
·
环境科学
·
纳米技术
·
食品安全
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产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Siqi Yu,Fudong Wang,Yuan Zhang,Xiaofeng Wang,Hongyu Zhao,Qiang Zheng
Autophagy inhibition induced by paraquat (PQ) poisoning is an important factor causing multi-organ damage, including renal tissue. Dihydrocapsaicin (DHC), the main active ingredient in chili peppers, was reported to induce autophagy, but its effect on PQ-induced AKI remains unclear. Rats were intraperitoneally injected with 25 mg/kg PQ to establish AKI rat model followed immediately by DHC administration (2.5, 5, or 10 mg/kg, i.p.). Rat renal tubular epithelial cells were exposed to PQ (300 μM) in combination with DHC (25, 50, or 100 μM) for 24 h. The results showed that DHC treatment attenuated PQ-induced renal injury and dysfunction while suppressing apoptosis and oxidative stress in vivo and in vitro . Furthermore, DHC administration induced autophagy—a protective mechanism against oxidative stress and apoptosis—as demonstrated by: 1) increased autophagosome/autophagolysosome formation, 2) upregulated LC3-II and autophagy-related gene 7 expressions and reduced p62 protein level. Blocking of autophagy by 3-methyladenine (3-MA) reversed the effects of DHC. Interestingly, transcription factor sex determining region Y-box 9 (Sox9) and autophagy inducer Sestrin2 RNA levels were increased by DHC treatment. Sox9 activated Sestrin2 transcription through direct binding to its promoter region. Sox9 or Sestrin2 silencing reversed the effects of DHC. Collectively, our findings demonstrate that DHC attenuates PQ-induced AKI via Sox9/Sestrin2 axis-mediated autophagy, indicating therapeutic utility of DHC against AKI.
研究领域:
药理学
·
毒理学
·
分子生物学
·
肾脏病学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Zejun Cao,Ruixi Xiao,Yuxia Chen,Jiangtao Dong,Xuezhong Du
The widespread global use of paraquat (PQ) is attributed to its high herbicidal efficacy and low cost, however, the unresolved issue of its fatal toxicity to humans after ingestion remains a major challenge. The nanocarriers MIL-101(Cr)@MoS 2 @Fe 3 O 4 (MMF) are synthesized to load PQ through the strong π–p electron coupling between the delocalized π electrons of PQ and the localized p electrons of S atoms of MoS 2 at the interface, and then encapsulated with chitosan to construct magnetically recyclable PQ nanoherbicides with excellent stability and biosafety. The release of PQ from the nanoherbicides in simulated human gastrointestinal environments is inhibited (“PASS0” logic gate) from the source, thereby enhancing user safety. Under natural sunlight conditions, the near-infrared photothermal effect of MMF weakens the strong interfacial π–p electron coupling between PQ and MMF, resulting in the release of PQ (“YES” logic gate). The PQ nanoherbicides maintain the herbicidal efficacy of bare PQ in practical applications. The residual nanoherbicides can be easily recovered using magnets, with the collection ratios of 97.8 % and 91.1 % in water and soil, respectively, at a magnetic field strength of 0.5 T. Seed dressing, cytotoxicity, and mouse models demonstrate that the nanoherbicides have excellent biosafety for both plant growth and PQ-ingesting users. The creative PQ nanoherbicides simultaneously address the challenging issues of user safety, herbicidal efficacy, and environmental friendliness from the source.
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Hui Shu,Tingrun Lai,Shanli Wang,Mengyao Li,Haoyu Li,Ting Chen,Xuechun Xiao,Yude Wang
Paraquat is a highly toxic herbicide, and its residue in food poses a huge threat to human health. High-sensitivity detection of trace paraquat is crucial but challenging. Herein, we theoretically predicted that FeCo-MOF materials with Fe and Co dual active sites have potential interactions with paraquat. Moreover, a porous FeCo-MOF materials with Fe and Co dual active sites were in-suit constructed on the surface of foam nickel by self-assembly strategy, and used for the electrochemical detection of paraquat. The electrochemical detection results indicate that the prepared FeCo-MOF foam nickel electrode exhibited ultra-high detection sensitivity (113.85 μA nM −1 cm −2 ) and trace detection limit (0.16 nM) in a wide range of paraquat concentration (1.00 nM–5.00 mM). In addition, we successfully used this FeCo-MOF foam nickel electrode for the detection of paraquat in apples, human serum, and fresh tea leaves (detection recovery is 97.00 %–99.99 %). Furthermore, the density functional theory (DFT) and X-ray photoelectron spectroscopy (XPS) confirm that Fe and Co are active sites for detecting paraquat, and the interaction between Fe and Co promotes highly sensitive detection of trace paraquat. Therefore, this work provides strategies and comprehension for designing and modulating active sites at the microscopic level to improve sensitivity for detecting toxic pesticides.
研究领域:
环境科学
·
分析化学
·
毒理学
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DOI
高影响因子
危险品化学品经营许可证（带存储）