Nanomaterials
·
2024
·Vol. 14
·期 24
·2022
·
IF 4.4
Qiongmei Mai,Yu Lu,Qianyu Cai,Jianglong Hu,Yunyou Lv,Yonglan Yang,Liqiang Wang,Yuezhao Zhou,Jie Liu
Sclerotinia sclerotiorumis one of the fungi that cause plant diseases. It damages plants by secreting large amounts of oxalic acid and cell wall-degrading enzymes. To meet this challenge, we designed a new pH/enzyme dual-responsive nanopesticide Pro@ZnO@Pectin (PZP). This nanopesticide uses zinc oxide (ZnO) as a carrier of prochloraz (Pro) and is encapsulated with pectin. When encountering oxalic acid released bySclerotinia sclerotiorum, the acidic environment promotes the decomposition of ZnO; at the same time, the pectinase produced bySclerotinia sclerotiorumcan also decompose the outer pectin layer of PZP, thereby promoting the effective release of the active ingredient. Experimental data showed that PZP was able to achieve an efficient release rate of 57.25% and 68.46% when pectinase was added or under acidic conditions, respectively. In addition, in vitro tests showed that the antifungal effect of PZP was comparable to that of the commercial Pro (Pro SC) on the market, and its efficacy was 1.40 times and 1.32 times that of the Pro original drug (Pro TC), respectively. Crucially, the application of PZP significantly alleviated the detrimental impacts of Pro on wheat development. Soil wetting experiments have proved that PZP primarily remained in the soil, thereby decreasing its likelihood of contaminating water sources and reducing potential risks to non-target organisms. Moreover, PZP improved the foliar wettability of Pro, lowering the contact angle to 75.06°. Residue analyses indicated that PZP did not elevate prochloraz residue levels in tomato fruits compared to conventional applications, indicating that the nanopesticide formulation does not lead to excessive pesticide buildup. In summary, the nanopesticide PZP shows great promise for effectively managingSclerotinia sclerotiorumwhile minimizing environmental impact.
研究领域:
植物病理学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2021
·
IF 5.221
Liu Can,Zhang Liming,Tan Li,Liu Yueping,Tian Weiqian,Ma Lanqing
In this study, we immobilized pectinase preparation on porous zeolite ZSM-5 as an enzyme carrier. We realized this immobilized enzyme catalyst, pectinase preparation@ZSM-5, via a simple combined strategy involving the van der Waals adsorption of pectinase preparation followed by crosslinking of the adsorbed pectinase preparation with glutaraldehyde over ZSM-5. Conformal pectinase preparation coverage of various ZSM-5 supports was achieved for the as-prepared pectinase preparation@ZSM-5. The porous pectinase preparation@ZSM-5 catalyst exhibited ultra-efficient biocatalytic activity for hydrolyzing the β-glycosidic bonds in the model substrate 4-nitrophenyl β-D-glucopyranoside, with a broad operating temperature range, high thermal stability, and excellent reusability. The relative activity of pectinase preparation@ZSM-5 at a high temperature (70 °C) was nine times higher than that of free pectinase preparation. Using thermal inactivation kinetic analysis based on the Arrhenius law, pectinase preparation@ZSM-5 showed higher activation energy for denaturation (315 kJ mol−1) and a longer half-life (62 min−1) than free pectinase preparation. Moreover, a Michaelis–Menten enzyme kinetic analysis indicated a higher maximal reaction velocity for pectinase preparation@ZSM-5 (0.22 µmol mg−1 min−1). This enhanced reactivity was attributed to the microstructure of the immobilized pectinase preparation@ZSM-5, which offered a heterogeneous reaction system that decreased the substrate–pectinase preparation binding affinity and modulated the kinetic characteristics of the enzyme. Additionally, pectinase preparation@ZSM-5 showed the best ethanol tolerance among all the reported pectinase preparation-immobilized catalysts, and an activity 247% higher than that of free pectinase preparation at a 10% (v/v) ethanol concentration was measured. Furthermore, pectinase preparation@ZSM-5 exhibited potential for practical engineering applications, promoting the hydrolysis of β-glycosidic bonds in baicalin to convert it into baicalein. This was achieved with a 98% conversion rate, i.e., 320% higher than that of the free enzyme.
研究领域:
生物化学
·
酶学
·
催化
·
材料科学
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生物技术
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DOI
PubMed
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Melani Purnika Mudugamuwa Arachchige,Taihua Mu,Mengmei Ma
Novel pectin derived from sweet potato residue was modified by high hydrostatic pressure (HHP)-assisted pectinase and then used for Pb 2+ removal from aqueous solutions. The removal characteristics and mechanisms were also investigated. Results showed that modified sweet potato pectin exhibited greater adsorption performances for Pb 2+ than that of natural ones, and showed excellent eco-friendly properties and good potential for adsorption of some other heavy metals (such as Cu 2+ ). The adsorption curves were much more conformed to Langmuir model, and the highest capacity for Pb 2+ adsorption was 263.15 mg/g with 1.00% pectin at pH 7. Chemical adsorption process of pectin for Pb 2+ absorption involved O-containing functional groups (O–H, COO-), cation exchange, and along with electrostatic interactions. Overall, the results in this study indicated that sweet potato pectin modified with HHP-assisted pectinase had the potential to become an environmentally friendly coagulant-flocculant agent for the heavy metal adsorption, especially for Pb 2+ .
研究领域:
食品科学
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环境科学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Talat M. Abdelrahman,Xueying Qin,Donglin Li,Ibrahim A. Senosy,Mohamed Mmby,Hu Wan,Jianhong Li,Shun He
Stimuli-responsive carriers as delivery systems of agrochemicals into plants can increase the utilization efficiency of pesticides against target pests and reduce hazards on nontarget living organisms and the environment. In the present work, prochloraz was loaded into mesoporous silica nanoparticles (MSNs) and coated by pectin on the particle surface as a gatekeeper to study the translocation and distribution of prochloraz@MSN-pectin (Pro@MSN-Pec) in rice plants. The results showed that Pro@MSN-Pec was successfully fabricated and had an average size of 70.89 nm and a good loading efficiency (30% w/w). The MSNs were labeled using fluorescein isothiocyanate (FITC) to track the distribution of the carriers in the rice plants. Confocal scanning analysis showed that MSN-FITC could be transported to all rice parts from the treated leaves or roots. The release of prochloraz (Pro) from Pro@MSN-Pec was pectinase-dependent. Compared with the commercial emulsifiable concentrate (EC formulation) of Pro, the Pro@MSN-Pec formula had a better uptake and translocation in rice plants as well as a longer duration and better antifungal activity against rice blast disease ( Magnaporthe oryzae ). The final residue level of Pro in the rice plants was lower than the maximum residue limits. These results suggest that the use of stimuli-responsive carriers as pesticide-delivery systems in plants can be potentially implemented in applied agriculture.
研究领域:
植物病理学
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农业科学
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纳米技术
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jinlong Li,Zihuan Zhao,Qihang Wei,Hongman Hou,Gongliang Zhang,Jingran Bi
This study integrates UiO-66-COOH metal–organic frameworks (MOFs) with pectin (Pec) to precisely release potassium cinnamate (PC) to develop a pectinase-responsive active fruit-preservation system (UiO-66-COOH@PC/Pec). Carboxylated UiO-66 with nanoporosity was fabricated to achieve a high loading amount of PC (235 mg·g −1 ) via the synergistic effects of hydrogen bonding, as shown by the loading kinetics and reduced density gradient analyses. A gatekeeper was established by grafting Pec onto the surface of the MOF (UiO-66-COOH@PC/Pec), thereby “locking” PC to prevent leakage. Moreover, an enzyme-responsive controlled-release behavior was developed under pectinase stimulation, achieving up to 95 % PC release within 9 h through Fickian diffusion and only 25.3 % in enzyme-free conditions by non-Fickian diffusion and matrix erosion. With significant long-term inhibiting activity against both bacteria and fungi, UiO-66-COOH@PC/Pec successfully extended the shelf life of strawberries from 2 to 5 days at 25 °C. Moreover, UiO-66-COOH@PC/Pec exhibited good water solubility and negligible toxicity, indicating that it can be easily removed from fruit surfaces and offers high consumer acceptance. These results provide a novel MOF-based controlled-release strategy for smart food-packaging applications.
研究领域:
食品科学与技术
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材料科学
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智能包装
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可控释放系统
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采后生物学与技术
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Ziying Guo,Yuting Zhou,Ping Wang,Qiang Wang,Bo Xu,Man Zhou,Li Cui,Ying Zhang,Yuanyuan Yu
The high energy consumption and pollution associated with traditional cotton fabric treatment have become increasingly serious concerns; thus, in this study, a synergistic cotton fabric treatment based on Novozymes pectinate lyase (NPL) scouring/ultraviolet light-mediated catalytic (UV/H 2 O 2 ) bleaching was innovatively proposed. This study investigated the degradation mechanisms of impurities in cotton fabrics, concluding that the oxidative bleaching reaction of morin follows first-order kinetics; in the UV/H 2 O 2 /NPL system, pectin degradation occurs through the β-elimination of NPL and oxidation by free radicals. Under optimized conditions—254 nm UV irradiation, 8 g⋅L −1 NPL, 30 g⋅L −1 H 2 O 2 , pH 9, and one-side irradiation for 45 min. The fabric whiteness could reach 79.04 %，but the fiber damage was severe, manifested by a 40 % decrease in polymerization and strength. After adjusting the process parameters, adjust the UV wavelength to 302 nm and add 6 g⋅L −1 of the activator (NOBS), whiteness of the fabric was about 76.7 % and strength retention rate was 96.38 %. This method overcomes the shortcomings of traditional pretreatment processes for cotton fabrics, under low temperature and alkaline conditions, low-strength damaged fabrics can be obtained, providing a new strategy for developing environmentally friendly pre-treatment processes for cotton fabrics.
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2025
·
IF 3.8
Yibing Yan,Songye Li,Shanshan Wang,Yifan Peng,Kaikun Pi,Bin Sun,Liang Cheng,Bing Liu,Fangxiang Song
BACKGROUND Rice sheath blight, caused by Rhizoctonia solani ( R. solani) poses a significant threat to global rice production. Although prochloraz (PRO) is an effective fungicide, its poor photostability and low water solubility severely limit its field performance. We developed a sustainable, stimuli-responsive delivery system to enhance PRO efficacy and minimize environmental risks. RESULTS Multilayered mesoporous nanoparticles (MSN@PMO) were synthesized via a hard-template route and converted into hollow periodic mesoporous organosilicas (H-PMO) by sodium carbonate etching. PRO was encapsulated in H-PMO and sealed with pectin, yielding a dual pH/pectinase responsive formulation (Pec@PRO-H-PMO, PPHP). PPHP exhibited sustained release for 144 h under acidic and enzymatic conditions, and showed markedly improved photostability, foliar adhesion and rainfastness compared with PRO technical concentrate (PRO TC) and PRO water emulsion (PRO EW). Bioassays demonstrated that PPHP exhibited 1.8 times greater antifungal activity against R. solani compared to PRO TC. Ecotoxicological analysis revealed 11.9 times higher zebrafish median lethal concentration (LC₅₀) values than PRO TC over 80% viability of human bronchial epithelial (BEAS-2B) cells at 18.4 mg/L, suggesting improved biosafety. CONCLUSIONS The dual pH/pectinase triggered release of PPHP enhanced pesticide utilization and improved safety. This strategy presents a novel approach for developing stimuli-responsive pesticide delivery systems, aiming to strengthen crop disease control efficiency and mitigate environmental risks. © 2025 Society of Chemical Industry.
研究领域:
农业化学
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农业纳米技术
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植物病理学
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农药制剂
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控释系统
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产品关联
DOI
PubMed
高影响因子
BMC BIOTECHNOLOGY
·
2019
·Vol. 19
·期 1
·1-11
·
IF 2.303
Zhang Ge,Li Shugui,Xu Yingbo,Wang Juan,Wang Fan,Xin Yuhua,Shen Zhong,Zhang Haibo,Ma Ming,Liu Haobao
Background Tobacco stalk (TS), a major agricultural waste abundant in pectin, has resulted in concerns about the need for its reuse. The nicotine in TS is considered a chemical that is to\xic and hazardous to the environment. Results In this study, Bacillus tequilensis CAS-MEI-2-33 was isolated from cigar wrappers to produce alkaline pectinase using TS. Subsequently, the medium and fermentation conditions for the production of pectinase by B . tequilensis CAS-MEI-2-33 were optimized. The optimal fermentation period, pH of the initial fermentation medium, concentration of TS, and inoculum amount for B . tequilensis CAS-MEI-2-33 were 40 h, 40 g/L, 7.0, and 3%, respectively. Under optimal conditions, the pectinase activity was 1370 U/mL. Then, the enzymatic properties, such as the optimum pH, reaction temperature, temperature stability, and effects of metal ions, were studied. The optimal pH was determined to be 10.0, indicating that the enzyme was an alkaline pectinase. The optimal temperature was 40 °C, and pectinase activity was stable at 40 °C. The Ag + metal ions were shown to remarkably promote enzyme activity. The pectinase was partly purified by ammonium sulfate precipitation, ion exchange chromatography, and Sephacryl S-100 chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and LC-MS/MS analyses were utilized to analyze the pectinase. Conclusions This study provided a new alkaline pectinase candidate and a new strategy for the use of TS.
研究领域:
生物技术
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酶学
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环境科学
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DOI
PubMed
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 8
·5149-5157
·
IF 3.9
Xiaoxiao Wu,Xushuo Yuan,Jiaxin Zhao,Decai Ji,Haiyang Guo,Wentao Yao,Xiaoping Li,Lianpeng Zhang
With the development of science and technology, efficient, fast and green methods are increasingly being pursued. The production of nanocellulose by green methods, such as bio-enzymes-assisted ultrasound treatment, has been the focus of many studies. However, the yield of cellulose nanocrystals prepared by this method is very low. In this paper, by pretreatment of microcrystalline cellulose (MCC), nanocellulose was prepared by heating and stirring + pectinase/cellulase + ultrasonic treatment (HSt – P/C – Ultr). The effects of the ratios of pectinase and cellulase and the hydrolysis time on the yield of nanocellulose were studied. FTIR, XRD, SEM, TEM and TG were used to determine the structure, crystallinity, morphology and thermal stability of nanocellulose. The results showed that optimal hydrolysis conditions were determined as a pectinase : cellulase ratio of 1 : 1, 90 min and 50 °C. The yield of nanocellulose was about 32.0%. The yield of pectinase cellulase = 1 : 1 was higher than that of microcrystalline cellulose (MCC) treated by a single bio-enzyme. This indicated that the synergistic effects of pectinase and cellulase have a certain effect on the formation of nanocellulose. During the preparation, the crystalline form of cellulose did not change. It was still cellulose I with a crystallinity of 73.5%, which is 9.50% higher than that of microcrystalline cellulose (MCC), a width of 20–50 nm, a high aspect ratio and a winding network structure. Therefore, nanocellulose prepared by this method is an ideal toughening material for manufacturing composite materials.
研究领域:
材料科学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）