FOOD CHEMISTRY
·
2012
·
IF 3.655
Qian Wu,Hengye Chen,Zhejuan Lv,Shuyi Li,Bei Hu,Yafei Guan,Bijun Xie,Zhida Sun
It has been reported that oligomeric procyanidins of lotus seedpod (LSOPC) is effective in the alleviation of Alzheimer’s disease and diabetes through its antioxidant and insulin-potentiating activities. This study investigated the anti-glycative activity of LSOPC in a bovine serum albumin (BSA)-glucose model. The level of glycation and conformational alterations were assessed by specific fluorescence, Congo red binding assay and circular dichroism. The results show that LSOPC has a significant anti-glycative activity in vitro and it can also effectively protect the secondary structure of BSA during glycation. LSOPC or catechin (a major constituent unit of LSOPC), were used to react with methylglyoxal. The structures of their carbonyl adducts were tentatively identified using HPLC-MS 2 . Their capacity to scavenge methylglyoxal suggested carbonyl scavenging as a major mechanism of antiglycation. Therefore, LSOPC could be helpful to prevent AGEs-associated diseases, and with the potential to be used as functional food ingredients.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
Food & Function
·
2022
·Vol. 13
·期 7
·4023-4031
·
IF 6.317
Shanshan Tie,Siyuan Xiang,Yannan Chen,Fengzhi Qiao,Weina Cui,Wentao Su,Mingqian Tan
Nanocarriers provide the possibility to overcome the low solubility, poor stability, and low bioavailability of functional factors. However, most nanocarriers do not directly participate in the corresponding effects of functional factors, such as treating inflammatory bowel disease but lack the means to control their size accurately. Herein, nanocarriers were prepared by a one-pot method, using food-grade antioxidant procyanidins, vanillin, and phycocyanin as raw materials. The strategy involved the Mannich reaction among the phenolic hydroxyl groups of procyanidins, the aldehyde groups of vanillin, and the amino groups of phycocyanin. The obtained nanocarriers displayed controllable sizes ranging from 130 to 750 nm, showing good antioxidant capacity in scavenging free radicals and were biocompatible to Caco-2 cells and RAW 264.7 macrophages. Nanocarriers also exhibited an inhibitory effect on cell damage induced by acrylamide and H2O2. Moreover, the designed nanocarriers could be used for delivering active ingredients such as lutein, which showed a uniform spherical distribution, high encapsulation efficiency, and good biocompatibility. This work provides a facile synthesis method to prepare food-grade nanocarriers with functional properties, which can be potentially used in the delivery of functional factors.
研究领域:
食品科学
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纳米技术
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生物医学工程
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药剂学
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DOI
PubMed
高影响因子
Food Bioscience
·
2022
·
IF 5.318
Gui-Li Huang,Tian-Tian Liu,Jia-Jia Ma,Ling-Xiang Sun,Si-Yao Sui,Xin-Yao Quan,Yu-Ning Wang
The harvested loquat fruits are prone to browning, leading to a decline in fruit quality. Inhibiting activity of polyphenol oxidase (PPO) is an important way to prevent browning. In this study, we evaluated the inhibitory impact of oligomeric procyanidins (OPC) on hydroxylation of monophenol and oxidation of o-diphenol catalyzed by PPO and effect of OPC on loquat fruits storage . The obtained results revealed that OPC could attenuate the activity of monophenolase and diphenolase. For monophenolase activity, the inhibition rate was observed to be dose-dependent with an IC 50 of 11.6 ± 3.4 μg/mL for the steady-state rate. For diphenolase activity, OPC inhibited PPO activity ( IC 50 = 15.75 ± 2.1 μg/mL) with a reversible and competitive mechanism and quenched fluorescence of PPO by forming OPC-PPO complex with one binding site in a static procedure. In addition, the data obtained from storage assays revealed that OPC could enhance the quality of harvested loquat fruits by attenuating the PPO activity, augmenting the content of total phenolics and flavonoids , and simultaneously delaying the browning of loquats. In this research, we identified a potent PPO inhibitor and expanded its potential as a food preservative.
研究领域:
食品科学
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植物生理学
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采后技术
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Shanshan Tie,Wentao Su,Xuedi Zhang,Yannan Chen,Xue Zhao,Mingqian Tan
The purpose of this study was to construct a delivery system using a microfluidic chip to protect procyanidins (PCs) and to achieve their pH-controlled release in simulated gastrointestinal fluid. The microfluidic chip was designed and fabricated to generate water-in-water-in-oil (W/W/O) templates for the preparation of sodium alginate/chitosan microparticles with a uniform size and core–shell structure, using an internal–external gelation method. Compared with free PCs, the stability of PCs embedded in microparticles was improved and a pH stimulus-responsive release of PCs from microparticles was observed under neutral pH conditions. The delivery system of microparticles was nontoxic and showed an inhibitory effect on the decrease of mitochondrial membrane potential in Caco-2 cells caused by H2O2 and acrylamide. This work provided a method for fabricating compact microfluidic chips to prepare a pH stimulus-responsive PCs delivery system with improved stability, which may have potential applications in the delivery of other nutrients.
研究领域:
药物递送系统
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微流控技术
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食品科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Fan Feng,Jing Gao,Li Chen,Shengwei Xiao,Guobo Huang
The co-delivery of curcumin (CUR) with other polyphenol bioactives often has synergistic benefits. However, conventional co-encapsulation carriers formed by precipitation frequently exhibit limited loading properties, particularly for bioactives with different solubilities. In this study, we designed a highly efficient vehicle for the co-delivery of hydrophobic CUR and hydrophilic procyanidins (PCs), adopting a multiple interactions–based strategy via a two-step flash nanoprecipitation (FNP) process. The optimal nanoparticles (NP), composed of a Fe II crosslinked PCs/CUR nanocore (NC) and a soy protein isolates (SPI) shell, showed high individual encapsulation efficiency (∼95%) and a total loading content of ∼38%. Compared with the conventional production process, FNP endowed our NP with a small size (133 nm) and good uniformity (polydispersity index = 0.267). Ultraviolet–visible and Fourier transform infrared spectra revealed multiple interactions (coordination and hydrogen bonding) among the carrier materials and bioactives, which played a central role in achieving excellent loading properties for both PCs and CUR. The core–shell structure of the NP greatly enhanced the thermal–light stability of the encapsulated PCs and CUR. Synergistic antioxidation effect was found between PCs and CUR, and the nano-formulation further increased the antioxidation efficiency. In vitro release experiments showed the favorable pH responsiveness of our NP in the vast gastrointestinal pH gradient effectively preventing the leakage of wrapped bioactives in the stomach pH environment, which benefits the intestinal delivery of the bioactives. Our nanoparticulate co-delivery system fabricated using the FNP process provides new insights into the formulation strategy of multiple bioactives and shows great potential for application in the fields of functional foods and dietary supplements.
研究领域:
纳米技术
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药学
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食品科学
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材料科学
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产品关联
DOI
高影响因子
BIOLOGICAL TRACE ELEMENT RESEARCH
·
2026
·
IF 3.6
An Hongmei,Zhang Lihong,Miao Jiale,Song Aiman,Zhang Jingzhuo,Maihemuti Wulike Baike,Cao Xianfeng,Wen Yongchang,Yan Ruiying,Ren Caijin,Bao Qianjiang,Wang Jiayu,Lei Yuhang
To investigate the protective effects of three antioxidants—lycopene (LP), proanthocyanidins (PC), and resveratrol (RES)—against Nano-TiO 2 -induced reproductive toxicity in male mice. A total of 110 healthy male ICR mice were randomly assigned to 11 experimental groups: the control group, the Nano-TiO 2 group, and combined treatment groups consisting of Nano-TiO 2 with low, medium, and high doses of LP, PC, and RES, respectively. Following 28 days of continuous intragastric administration, epididymal tissues were collected for assessment of semen quality, while testicular tissues were utilized to evaluate histopathological alterations, testicular functional enzyme activities, and markers of oxidative stress. Compared with the control group, after intragastric administration of Nano-TiO 2 , the testicular and epididymal coefficients of mice decreased, the sperm count, sperm progressive motility, and sperm motility rate significantly decreased ( P < 0.05), the sperm malformation rate significantly increased ( P < 0.05), the number of spermatogenic cells in the testicular spermatogenic tubules decreased, and the Johnsen score significantly decreased ( P < 0.05). The activities of acid phosphatase (ACP), alkaline phosphatase (AKP), lactate dehydrogenase (LDH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione (GSH) decreased significantly ( P < 0.05). The level of malondialdehyde (MDA) was significantly increased ( P < 0.05). After the intervention with the three antioxidants, compared with the Nano-TiO 2 group, the testicular and epididymal coefficients increased. Compared with other doses, medium-dose LP, medium-high-dose PC, and all doses of RES significantly increased the forward motility and sperm motility of mice and significantly reduced the sperm deformity rate( P < 0.05). In addition, various doses of LP, medium - high- doses of PC, and low- doses RES significantly improved pathological damage caused by Nano-TiO 2 , such as testicular tissue structural disorder and decreased levels of spermatogenic cells and sperm. The activities of ACP, LDH, SOD, CAT, GSH, and GPx increased significantly ( P < 0.05), and the level of MDA in the testicular tissue decreased significantly. LP, PC, and RES had antagonistic effects on male reproductive toxicity induced by Nano-TiO 2 , the prominent effects of PC on the testicular organ coefficient and LP, especially 20 mg/kg LP, in improving semen quality and the expression of testicular functional enzymes in male mice need to be given due attention.
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PubMed
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2020
·Vol. 246
·期 5
·1017-1029
·
IF 2.366
Tian Ruochen,Suo Hao,Zhang Shuting,Sun Baoshan
A series of thiol-nucleophiles, captopril, l -cysteine, and tiopronin, were introduced to the degradation reaction of grape seed extracts and the eight new bioactive flavan-3-ol derivatives were observed. Then, these derivatives were successfully separated and prepared in large quantity by high-speed counter-current chromatography (HSCCC) and semi-preparative HPLC under the optimized separation conditions. The appropriate two solvent systems consisted of n- hexane – ethyl acetate – methanol – water (0.05:1.5:0.5:1.2, v/v/v/v) and n- hexane – ethyl acetate – water (1:10:10, v/v/v) with head – tail mode were used for HSCCC two-step separation of captopril degradation products. N- butanol – ethyl acetate – methanol – water (2:4:0.5:6, v/v/v/v) with a combination of tail – head and head – tail elution modes in HSCCC run of l- cysteine degradation products, and n- hexane – ethyl acetate – methanol – water (0.15:1.6:0.4:1, v/v/v/v) was applied with head – tail and tail – head elution modes in HSCCC separation of tiopronin degradation products. The structures of these derivatives were confirmed by MS and 1 H NMR analysis. Moreover, a systematic comparison of antioxidant activity of all the eleven degradation products was first implemented and found that most of the derivatives had stronger antioxidant activity than free catechins.
研究领域:
植物化学
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分离技术
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药理学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Qing Zhang,Yujin Yang,Yannan Chen,Shuoshuo Ban,Shaobin Gu,Fang Li,Mengmeng Xue,Jianrui Sun,Xin Li,Shanshan Tie
The purpose of this study was to prepare a pH-responsive microgel for co-delivering Weizmannia coagulans 99 (BC99) and procyanidins (PCs) to enhance the survival rate and tolerance of probiotics in complex micro-environment. The effects of different concentrations of PCs on the properties of microgels were optimized, and found that the spherical microgels had higher encapsulation efficiency (90.27 ± 2.51%) and smaller size when the concentration of PCs was 20 μg/mL. The interaction among PCs, pectin and protein could effectively improve the survival rate of BC99 under different pH, bile salt, digestive enzyme and temperature conditions, maintain their stability in acidic gastric fluid, and realize the release of probiotics in neutral intestinal fluid. Moreover, the microgel was able to protect BC99 against H 2 O 2 and antibiotics. This work provides a pH-responsive co-loaded microgels for BC99 and PCs, and has the potential in the loading and delivery of other probiotics and polyphenols.
研究领域:
微生物学
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食品科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Feng Nianjie,Tang Fei,Hu Chuanqin,Cheng Lei,Lv Zhejuan,Shen Yang,Li Wei,Xiao Gengsheng,Dong Hao,Wu Qian
Lactic acid bacteria (LAB) have already been used as fermentation strains to enhance the antioxidant capacity of polyphenols. Antioxidant capacity is one of the most important factors to inhibit advanced glycation end product (AGE) formation and could LAB increase the inhibitory capacity of procyanidins on AGEs formation? It was surprising that opposite results were obtained both in simulated food processing and gastrointestinal digestion systems. After incubation with Lactobacillus plantarum (L. plantarum), litchi pericarp oligomeric procyanidins (LPOPCs) were bioconverted to several phenolic acids, which increased the antioxidant activity as expected. However, antiglycation ability and trapping carbonyl compounds capacity both weakened and it might be the primary reason for decreasing the inhibitory effect on AGE formation. Furthermore, it was found that LPOPCs incubated with L. plantarum inhibited the activity of digestive enzymes and thus decreased the digestibility of glycated protein. Our study systematically proposed for the first time that procyanidins bioconversion is an effective means to improve the antioxidant activity but has no remarkable promoting effect on AGEs inhibition.
研究领域:
食品科学
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生物化学
·
医学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Shasha Huang,Kuiyou Wang,Yi Ma,Zuzhe Wang,Mingqian Tan
Sea cucumber peptide (SCP) reacted with procyanidins and vanillin to form a food-grade nanoparticles (SPNs) via the Mannich reaction. After encapsulation astaxanthin (AXT), the SPNs was attached to a SCP-modified hyaluronic acid (HA) hydrogel to construct AXT@SPNs/HS. The AXT@SPNs/HS exhibited an average size of approximately 1281 nm, while the AXT@SPNs had an average size of 510 nm. The loading capacity of AXT in AXT@SPNs and AXT@SPNs/HS were 6.81 ± 0.10 and 6.56 ± 0.19 μg/mg, respectively. Sustained release of AXT was found during AXT@SPNs/HS digestion and HA-SCP hydrogel provided a significant advantage in protecting AXT in acidic conditions. AXT@SPNs/HS could effectively target RAW264.7 macrophages and mitigated oxidative stress inflammatory response caused by alcohol. AXT@SPNs/HS alleviated acute alcoholic liver damage in mice model. The results illustrated AXT@SPNs/HS might become a candidate with intestinal sustained release to enhance its bioavailability.
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PubMed
高影响因子
Journal of Cleaner Production
·
2019
·
IF 6.395
Kexin Hou,Meili Bao,Lu Wang,Hua Zhang,Lei Yang,Haitian Zhao,Zhenyu Wang
In this study, in order to simultaneously extract procyanidins and essential oil from pinecones of Pinus koraiensis , aqueous enzymatic pretreatment ionic liquid–lithium salt based microwave–assisted extraction (AEILLME) with a modified hydrodistillation concatenated Soxhlet extraction apparatus was proposed. 1-Butyl-3-methylimidazolium bromide solution was selected as extraction solvent , while LiCl and mixture of cellulase and pectinase (1:1, w/w) as additives by single factor experiment. Other parameters that affect the yield of target components were investigated and optimized by Plackett–Burman design and Box–Behnken design. The obtained optimal conditions were: microwave irradiation power 581.49 W, microwave irradiation time 15.95 min and liquid–solid ratio 11.91 mL/g. Under the optimized conditions, the predicted yields of oligomeric procyanidins, polymeric procyanidins and essential oil were 13.02, 32.19 and 7.76 mg/g, respectively. Analysis of chemical constituents in the essential oil was performed on gas chromatography–mass spectrometry and there was no significant difference on the components of essential oil obtained by proposed method and traditional methods. Ionic liquids were successfully recycled and reused, which could lower the process cost and being beneficial to industrial production. This study provides an approach with good prospects for the application in extracting active components from natural plant material.
研究领域:
化学工程
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分析化学
·
植物化学
·
天然产物
·
提取技术
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产品关联
DOI
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2022
·
IF 7.419
Yao Liu,Ruifang Sun,XiaoPing Lin,Lanlan Wu,Hengying Chen,Siwen Shen,Yan Li,Yuanhuan Wei,Guifang Deng
Gestational Diabetes Mellitus (GDM) has an effect on the health of pregnant women and fetuses. Procyanidins (PA) is a flavonoid with anti-diabetic activity, but its effects and mechanisms on GDM have not been defined. Herein, we studied further the functions and mechanisms of PA on insulin resistance (IR) in GDM mice, as well as on postpartum and offspring mice. GDM mice model was built by feeding a high-fat-high-sucrose diet, and PA intervention (27.8 mg/kg/d) was performed from 4 weeks before pregnancy to delivery. Intestinal flora deficient (IFD) mice model was established by broad spectrum antibiotics. PA decreased the gestational weight gain, and the levels of fasting blood glucose, insulin, homeostasis model of assessment for IR index, yet increased the levels of HOMA for insulin sensitivity index. Interestingly, in IFD mice the effect of PA on improving IR was significantly weakened. PA inhibited inflammation by decreasing the levels of IL-6, TNF-α, IL-17 and CRP, which also been blocked in the IFD mice. Moreover, PA improved glycometabolism and reduced the secretion of inflammatory factors and hepatic inflammation infiltration of mice at 4 weeks postpartum, but had no significant effect on offspring mice. Mechanistically, PA treatment suppressed the nuclear factor‐κB (NF‐κB) p65 nuclear translocation and nucleotide‐binding domain like receptor protein 3 (NLRP3) inflammasome activation. In vitro studies, 4-hydroxyphenylacetic acid and 3-(4-hydroxyphenyl) propionic acid, main intestinal flora metabolites of PA restrained NF‐κB/NLRP3 activation. In conclusions, PA improved IR via NF-κB/NLRP3 pathway in GDM and postpartum mice, which partly through its metabolites by gut microbiome.
研究领域:
药理学
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内分泌学
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免疫学
·
微生物学
·
代谢组学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）