DYES AND PIGMENTS
·
2020
·
IF 4.613
He Cheng,Yiwen Zhao,Huimin Xu,Yifan Hu,Li Zhang,Gang Song,Zhiyi Yao
In this report, a water soluble perylene diimide derivative (PDI-Glu) was designed and prepared for rapid and visual detection of protamine. The sensing performance and mechanism of this probe toward protamine was investigated systematically by absorption and emission spectroscopic techniques. The fluorescence quenching caused by the aggregation of PDI-Glu in the presence of protamine could be outputted by spectrometer and observed by naked eyes. This method showed an excellent selectivity towards protamine in comparison of other analytes. It also featured good water solubility, large operable range, fast and visualized detection. The detection limit of protamine was calculated to be 0.09 μg mL −1 . Finally, this probe can be also applied in serum samples and monitoring trypsin activity.
研究领域:
分析化学
·
生物化学
·
材料科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Pirah Ayaz,Xi Liu,Yuyun Yu,Shuangfei Xiang,Shujun Zhao,Feiya Fu,Hongyan Diao,Xiangdong Liu
Covalent immobilization of antibacterial peptides (APs) onto silk protein-based materials has always been a challenge due to the lack of green and efficient macromolecular cross-linkers. Here, we proposed a dialdehyde polysaccharide cross-linker oxidized from pullulan for grafting a natural AP protamine (PM) onto silk fiber surface through a simple cold pad-batch process. The oxidized pullulan (OP) was linked to silk fiber surface through Schiff reaction and used for mediated cross-linking of PM also via Schiff base linkages. This modification introduced abundant PM guanidine groups on the fiber leading to much-desired antibacterial activity, and considerable improvement in the moisture transfer properties and shade depth. FTIR, XPS, SEM studies confirmed the presence of PM and the cross-linking structure between the polysaccharide and peptides on the fiber surfaces. The antibacterial activity imparted by this process was retained even after 20 washing and 50 rubbing cycles proving versatility and durability. Further, the process did not affect other critical silk properties such as appearance, tensile strength, biological safety, etc. Immobilization of PM onto silk fibers through this novel green polysaccharide cross-linker makes silk more appealing and usable and could also enlighten the attempts of cross-linking other protein materials.
研究领域:
材料科学
·
纺织工程
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Fangfang Xue,Fei Qu,Wenli Han,Lian Xia,Jinmao You
Metal nanoclusters protected by glutathione (GSH) have attracted a wide attention due to the unique aggregation-induced emission (AIE) feature. However, the “trigger” effects of ethanol, temperature, pH values, and metal ions may restrict the application of these particles. In this work, the amino modified silicon nanoparticles (SiNPs) and GSH-capped gold nanoclusters (GSH-AuNCs) can self-assemble into well-defined spherical particles due to the electrostatic interaction . As a result, the unique aggregation-induced emission enhancement (AIEE) of GSH-AuNCs arises at 570 nm, and the SiNPs keep their own blue fluorescence at 450 nm, so a novel nanohybrid probe (SiNPs@GSH-AuNCs) with dual-emission property has been constructed. When protamine is added to SiNPs@GSH-AuNCs, the cationic protamine can compete with SiNPs and absorb onto the surface of GSH-AuNCs, which inhibits the self-assembly and leads to the fluorescence quenching of GSH-AuNCs; while trypsin can catalyze the hydrolysis of protamine, the self-assembly starts again, producing the AIEE recovery. In the whole process, the SiNPs act as an internal standard and their emission stays constant. By means of the fluorescence intensity ratios I 570 /I 450 , the linear range of protamine is from 0.15 to 3.00 μg mL −1 with the limit of detection (LOD) of 0.07 μg mL −1 , and trypsin shows a linear response in the range from 10 to 100 ng mL −1 with LOD of 4.50 ng mL −1 . Furthermore, this strategy exhibits good sensitivity and selectivity , and has been further validated by applying it for the determination of protamine and trypsin in serum samples.
研究领域:
生物化学
·
纳米医学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Zhirun Ji,Zhaoyang Shang,Muhammad Sohail,Pei Wang,Bingzhi Li,Xing Zhang,Guoxiang Chen
Protamine, an arginine-rich polycationic peptide, is a natural food preservative and the only approved antidote to heparin overdose. The detection of protamine is meaningful; however, the detection of protamine is difficult due to the lack of aromatic amino acids in its composition . Herein, we proposed a fluorometric biosensor for the sensitive detection of protamine based on the protamine-mediated regulation of CRISPR/Cas12a collateral cleavage. In the absence of protamine, the DNA probe specifically hybridizes with the Cas12a-crRNA complex, causing the activation of Cas12a. In the presence of protamine, positively charged protamine tightly binds to negatively charged DNA probes through electrostatic interaction , so that the DNA probe cannot hybridize with the Cas12a-crRNA complex. Using a short ssDNA modified with fluorophore and quencher as a reporter, the information of protamine concentration is transduced into DNA information and sensed in a signal turn-off manner. The linear range of this method in protamine detection is 0.04–3.2 μg/mL, yielding a detection limit of 0.03 μg/mL, and the entire sampling-to-response time is about 1 h. This biosensor provides a rationale for using the programmable nuclease Cas12a to detect protamine, and further advances the application of CRISPR technology in bioanalyses.
研究领域:
生物化学
·
分子生物学
·
医学检测
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产品关联
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2012
·
IF 2.367
Jing-Yun Wang,Cui-Li Ma,Yong-Ming Bao,Pin-San Xu
Lipase from Burkholderia cepacia was encapsulated inside zirconia particles by biomimetic mineralization of K 2 ZrF 6 induced with protamine, a natural cationic protein. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FT-IR) were employed for the characterization of the novel immobilized lipase. SEM and TEM images showed that both the zirconia particles with and without lipase have good spherical structures with average particle sizes of 150 nm. Fluorescence microscopy demonstrated that the lipase was indeed encapsulated inside the zirconia particles. The maximum immobilization capacity of the zirconia particles was 0.15 units/mg under optimum immobilization conditions. Biochemical characterization showed that the encapsulated lipase could retain most of its initial activity. Compared with free lipase, the encapsulated lipase exhibited improved thermal, pH, and recycling stabilities. After 8 weeks of storage, no substantial loss in catalytic activity was observed for the encapsulated lipase. The conversion of the kinetic resolution of ( R,S )-1-phenylethanol with vinyl acetate as acetyl donor catalyzed by zirconia-immobilized lipase reached 49.9% with higher ee s of 99.9% under the following optimal conditions: octane as solvent, 0.1 M ( R,S )-1-phenylethanol, 70 mg immobilized lipase, 180 rpm, 50 °C for 48 h. After 6 cycles (288 h), the conversion and ee s were still 43% and 85%, respectively.
研究领域:
生物化学
·
材料科学
·
酶工程
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2020
·
IF 4.389
Jin-Hua Xue,Ke-Ping Xiao,Yong-Sheng Wang,Ling Liu,Jia-Qian Li,Mei Li,Yue-Ning Qu,Xi-Lin Xiao
In this study, we confirmed that protamine-templated gold nanoclusters (PRT-AuNCs) exhibit aggregation-induced emission properties (AIE-PRT-AuNCs). 1-Hydroxypyrene (1−OHPy) further induced the aggregation of AIE-PRT-AuNCs via hydrogen bonding and electrostatic and hydrophobic interactions, resulting in the aggregation-induced photoluminescence enhancement of AIE-PRT-AuNCs. 9-Hydroxyphenanthrene was able to decrease the background signal, thus increasing the sensitivity of the method. Based on these findings, a cost-effective, highly sensitive and selective strategy was proposed for the quantitative detection of 1−OHPy. This method displayed a wide linear range of 0.924 – 74.1 nmol/L with a low detection limit of 0.277 nmol/L, showing great potential for the monitoring of 1−OHPy in human urine. This strategy may provide a theoretical basis for future studies of the AIE properties of metal nanoclusters and their applications in the field of chemical and biological sensing.
研究领域:
分析化学
·
生物医学工程
·
环境科学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Lihua Liu,Jianan Dai,Yuan Ji,Baoxing Shen,Xing Zhang,Robert J. Linhardt
Herein, we designed a BODIPY-based probe (BZA-BOD@ZIF-90) with good stability through the encapsulation of metal-organic frameworks (MOFs). BZA-BOD@ZIF-90 can selectively detect protamine based on an aggregation-induced emission (AIE) effect. In the present strategy, the designed BZA-BOD@ZIF-90 showed excellent fluorescence response to protamine with a low detection limit of 0.07 μg/mL and the detection was not disturbed by other possible competing substances. Compared with previous methods, BZA-BOD designed by this method is simpler to obtain, and also can achieve sensitive and accurate determination of protamine. Subsequently, the nanocomposite BZA-BOD@ZIF-90 was successfully applied to detect protamine spiked in human serum. In addition, due to the strong binding effect of heparin on protamine, when heparin was added to the complex, the fluorescence intensity of the BZA-BOD@ZIF-90 weakened, thus, it can also be utilized for heparin detection. The medical application of protamine and heparin suggests that this fluorescent molecular device has prospects for certain clinical applications.
研究领域:
分析化学
·
材料科学
·
生物医学工程
·
荧光探针
·
药物检测
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Xiaoyun Lin,Zhenmao Zhu,Chengfei Zhao,Shaoguang Li,Qicai Liu,Ailin Liu,Liqing Lin,Xinhua Lin
In this study, we developed a simple, sensitive and label-free colorimetric sensor for trypsin and its inhibitor using oxidase mimicking activity of protamine-stabilized platinum nanoparticles units (Pro-PtNPs units). Protamine is designed to stabilize PtNPs and served as the substrate of trypsin hydrolysis. The results show that protamine combines several PtNPs together to form active units, which have stronger oxidase mimicking activity than other PtNPs. In addition, the Michaelis constant of the Pro-PtNPs units is smaller than negatively charged citrate capped PtNPs, suggesting that the positively charged Pro-PtNPs units have better affinity with 3,3′,5,5′-tetramethylbenzidine (TMB), which is inconsistent with previous reports. The protamine stabilizer could be hydrolyzed in the presence of trypsin, and the Pro-PtNPs units would be decomposed and aggregated, resulting in a decrease of catalytic activity of Pro-PtNPs units. The color of the solution would be reduced according to the decrease of catalytic activity, so the catalytic activity is linearly related to UV absorbance. The linear range of trypsin detected by this new method is 0.06 μg/mL˜0.6 μg/mL, and the detection limit down to 0.03 μg/mL. Pro-PtNPs units have been successfully applied to the determination of serum trypsin and screening of inhibitors.
研究领域:
生物化学
·
纳米医学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 11
·1-9
·
IF 5.479
Xue Jin-Hua,Liu Ling,Wang Yong-Sheng,Li Jia-Qian,Li Mei,Qu Yue-Ning,Li Le
This work describes a method for the determination of 1-hydroxypyrene (OH-Py) via aggregation-induced quenching of the emission of protamine-coated gold nanoclusters using 9-hydroxyphenanthrene (OH-Phe) as a sensitizer to boost the emission efficiency of nanoprobe. Under optimum conditions, the drop in fluorescence intensity at excitation/emission wavelengths of 300/596 nm is proportional to the concentrations of OH-Py in the range from 1.0 to 65 nM. The relative standard deviations are 4.2, 2.4 and 1.9% (for n = 11) at concentration levels of 8.0, 32 and 48 nM of OH-Py, respectively. The detection limit is 0.3 nM which is much lower than that of some previously reported methods. The recoveries from urine samples spiked with OH-Py ranged between 94.4 and 98.8%.
1-Hydroxypyrene (OH-Py) can trigger the aggregation of protamine-gold nanoclusters (PRT-AuNCs), resulting in the emission quenching of PRT-AuNCs. 9-Hydroxyphenanthrene (OH-Phe) can boost the emission efficiency of nanoprobe. Thereby, a highly sensitive assay of OH-Py was established.
研究领域:
分析化学
·
环境医学
·
纳米生物学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
May S. Freag,Wedad M. Saleh,Ossama Y. Abdallah
In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY). Tripterine (TRI) is a herbal drug widely investigated as a potential anticancer candidate against various types of cancers. However, clinical use of TRI is handicapped by its low oral bioavailability. To surmount TRI pharmaceutical obstacles, TRI phytosomes (TRI-PHY) were prepared using solvent evaporation technique then coated with a protamine layer via electrostatic assembly process. The developed PRT-TRI-PHY showed a nano-metric size of 250 nm and positive zeta potential (+21.6 mV). Sponges loaded with PRT-TRI-PHY demonstrated a sustained release profile with superior mucoadhesion characteristics compared with the counterparts loaded with uncoated TRI-PHY. The ex-vivo permeation study via chicken pouch mucosa revealed that sponges loaded with PRT-TRI-PHY demonstrated 2.3-folds higher flux value compared with sponges loaded with uncoated TRI-PHY. Additionally, in-vivo pharmacokinetic study in healthy rabbits revealed the significantly higher bioavailability of PRT-TRI-PHY compared with TRI-PHY with relative bioavailability of 244%. Conclusively, mucoadhesive CS-HPMC sponges loaded with a novel mucopenetrating nanocarrier, PRT-TRI-PHY, could significantly improve the absorption of tripterine via buccal mucosa which would be of prime importance for its clinical utility.
研究领域:
药剂学
·
生物医学工程
·
药物传递系统
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2024
·
IF 5.3
Huijing Cong,Jing Hu,Jing Wang,Baiyu Chang,Rongtao Li,Xinran Cui,Chenghao Zhang,Hongyu Ji,Congcong Lin,Jingling Tang,Jiaxin Liu
Parkinson’s disease confronts challenges in drug delivery due to the blood-brain barrier. Intranasal delivery bypasses the blood-brain barrier for improved drug bioavailability, yet narrow nasal space and brief retention time hinder clinical applicability. We conducted a Bromocriptine Mesylate-loaded PLGA nanoparticles co-modified with low molecular weight protamine (LMWP) and lactoferrin (Lf) (LMWP/Lf-BCM-NPs) for nose-to-brain delivery. The resulting LMWP/Lf-BCM-NPs were uniform spheres with an average size of 248.53 ± 16.25 nm and zeta potential of −2.63 ± 0.74 mV. Fourier transform infrared spectroscopy confirmed LMWP and Lf attachment. The co-modified nanoparticles showed improving cellular transport and good viability. The LMWP/Lf-BCM-NPs showed increased brain targeting efficiency in mice. In haloperidol-induced Parkinson mouse models, the LMWP/Lf-BCM-NPs showed increased brain targeting efficiency, enhanced behavioral regulatory effects, enhanced antioxidant effects and neuroprotection effects. This study paves the way for a novel, non-invasive brain-targeted therapy, offering a promising avenue for Parkinson’s disease clinical treatment.
研究领域:
药剂学
·
神经科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Qiaozhen Bao,Dan Lin,Yaoran Gao,Lina Wu,Jinhua Fu,Khuslen Galaa,Xinhua Lin,Liqing Lin
Herein we develop a novel “off–on-off” fluorescent nanosensor for the ultra-sensitive detection of protamine and trypsin based on the as-prepared nitrogen and sulphur doped carbon dots (N,S-CDs) and gold nanoparticles (AuNPs). N,S-CDs were treated as fluorophore, while AuNPs were treated as fluorescence absorber. By experimental studies, the fluorescence quenching mechanism of N,S-CDs by AuNPs was inner-filter effect (IFE). Upon addition of protamine to the mixed aqueous of N,S-CDs/AuNPs, the electrostatic interaction and Au-N bond between protamine and AuNPs resulted in AuNPs aggregation, and the quenched fluorescence of N,S-CDs could be recovered. Protamine is rich in arginine and can be specifically hydrolyzed by trypsin, resulting in the deaggregation of AuNPs and further quenching the fluorescence of N,S-CDs. Thus, we can determine the concentration of protamine and trypsin by measuring fluorescence intensity of N,S-CDs. Under the optimized conditions, the fluorescence intensity showed a good linear relationship with protamine concentration of 20–260 ng/mL with the detection limit of 4.7 ng/mL. Meanwhile, quantitative determination of trypsin in a range from 10 to 140 ng/mL with the detection limit of 4.3 ng/mL can be achieved in this fluorescent nanosensor. This fluorescent nanosensor also showed excellent selectivity, and we successfully applied it for the detection of protamine and trypsin in human urine samples. Based on the above findings, we conclude that this novel “off–on-off” fluorescent nanosensor has great potential in clinical diagnosis.
研究领域:
分析化学
·
纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）