BIOMACROMOLECULES
·
2018
·
IF 5.738
Jie Zhou,Aisha Roshan Mohamed Wali,Shengnan Ma,Yiyan He,Dong Yue,James Zhenggui Tang,Zhongwei Gu
In the progress of designing a gene carrier system, what is urgently needed is a balance of excellent safety and satisfactory efficiency. Herein, a straightforward and versatile synthesis of a cationic guanidine-decorated dendronized pullulan (OGG3P) for efficient genetic photodynamic therapy was proposed. OGG3P was able to block the mobility of DNA from a weight ratio of 2. However, G3P lacking guanidine residues could not block DNA migration until at a weight ratio of 15, revealing guanidination could facilitate DNA condensation via specific guanidinium-phosphate interactions. A zeta potential plateau (∼+23 mV) of OGG3P complexes indicated the nonionic hydrophilic hydroxyl groups in pullulan might neutralize the excessive detrimental cationic charges. There was no obvious cytotoxicity and hemolysis, but also enhancement of transfection efficiency with regard to OGG3P in comparison with that of native G3P in Hela and HEK293T cells. More importantly, we found that the uptake efficiency in Hela cells between OGG3P and G3P complexes was not markedly different. However, guanidination caused changes in uptake pathway and led to macropinocytosis pathway, which may be a crucial reason for improved transfection efficiency. After introducing a therapeutic pKillerRed-mem plasmid, OGG3P complexes achieved significantly enhanced KillerRed protein expression and ROS production under irradiation. ROS-induced cancer cells proliferation suppression was also confirmed. This study highlights the guanidine-decorated dendronized pullulan could emerge as a reliable nonviral gene carrier to specifically deliver therapeutic genes.
研究领域:
药学
·
生物医学工程
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Zeyu Qin,Xiwen Jia,Qian Liu,Baohua Kong,Hao Wang
In this study, electrospun chitosan/pullulan composite nanofiber films were crosslinked by two green methods (heating and cinnamaldehyde). With the increase of chitosan content, the morphology results indicated that the nanostructures of thermal crosslinking chitosan/pullulan (TCP) and cinnamaldehyde crosslinking chitosan/pullulan (CCP) nanofiber films became more stable. The decreases in weight loss (83.65 % to 43.85 % for TCP and 23.42 % to 15.58 % for CCP) and the increases in water contact angle (31.8° to 54.1° for TCP and 83.65 % to 43.85 % for CCP) confirmed the improved water stability. The decreases in water vapor permeability indicated that the crosslinking processes significantly improved the barrier properties of the films. Mechanical and thermal properties of TCP and CCP films were enhanced respectively. Furthermore, the corresponding properties of CCP films were more excellent than TCP films. FTIR results indicated the occurrence of Maillard reaction during the thermal crosslinking process and Schiff base reaction during the cinnamaldehyde crosslinking process.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2019
·
IF 3.621
Shangji Li,Wei Dai,Zheng-Zhi Yin,Jun Gao,Datong Wu,Yong Kong
The past decades have witnessed the development of mesoporous silica as an attractive carrier for controlled drug delivery due to its outstanding mesoporous feature, facile surface functionalization and good biocompatibility. In this work, NH 2 grafted mesoporous silica nanoparticles (NH 2 -MSN) were first synthesized for the loading of 5-fluorouracil (5-FU), and then the drug loaded NH 2 -MSN were coated with oxidized pullulan (oxPL) via Schiff base reaction for the encapsulation of 5-FU. The resultant drug carrier, 5-FU/MSN/oxPL, was fully characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Dynamic light scattering (DLS), 1 H NMR, Fourier transform infrared (FT-IR) spectroscopy and nitrogen adsorption/desorption isotherms, respectively. Finally, controlled delivery of 5-FU from the carrier was investigated. Since the acylhydrazone bond between MSN and oxPL is prone to be hydrolyzed under acidic conditions, the encapsulated 5-FU is easily released from the MSN/oxPL carrier in acidic environment due to the separation of oxPL coating from MSN. Cytotoxicity of the developed carrier was also evaluated using LO2 and HepG2 cell lines by conventional CCK-8 assay.
研究领域:
材料科学
·
药学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Xiaoliang Qi,Qiankun Zeng,Xianqin Tong,Ting Su,Lei Xie,Kai Yuan,Jianxiong Xu,Jianliang Shen
Both secondary pollution and the low mechanical strength of adsorbents have severely impeded the practical application of adsorption methods in the dye wastewater treatment. In this work, we innovatively synthesized a composite hydrogel adsorbent by incorporating polydopamine (PDA) and montmorillonite (MMT) into the pullulan hydrogel matrix for dye adsorption. First, the successful formation of the resultant adsorbents was verified by Fourier-transform infrared spectroscopy and scanning electron microscope elemental mapping analysis. Then, several physicochemical properties (such as thermal and swelling properties, microarchitecture, and mechanical strength) of the five prepared adsorbents (PM1–PM5) were investigated. These results demonstrated the adsorbents had tunable properties, which can be achieved by adjusting the PDA/MMT mass ratio. Next, the dye adsorption performance was systematically explored. The resultant adsorbent (PM3) had a maximum adsorption capacity of 112.45 mg/g and its adsorption data was best described by a Langmuir isotherm and pseudo-second-order kinetic. Finally, the adsorption mechanisms and potential commercial practicability of the adsorbent were studied. Altogether, the designed adsorbent could effectively avoid generating the secondary pollution typical of adsorbents and it displayed excellent mechanical strength, thus opening up a new horizon in mitigating environmental pollution from textile industries.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2011
·
IF 3.044
Huanan Li,Jing Yang,Xiaona Hu,Jie Liang,Yujiang Fan,Xingdong Zhang
To accomplish ideal wound dressing, hydrogels based on a natural polysaccharide, pullulan were synthesized by chemical cross-linking. The tensile strengths of the hydrogel films (1 mm thick) were determined to range from 0.663 to 1.097 MPa in proportion to cross-linking degrees and water contents. The swelling study of the hydrogels in water showed remarkable water absorption property with swelling ratio up to 4000%, which provided the hydrogel with quick hemostatic ability and prevent the wound bed from accumulation of exudates. The water vapor transmission rate and water retention of the hydrogels were found to be in the range of 2213–3498g/m 2 /day and 34.74–45.81% (after 6 days), indicating that the hydrogel can maintain a moist environment over wound bed, which could prevent the dehydration of the wound bed and prevent the scab formation. Biocompatibility test revealed that the hydrogels were not cytotoxic. The hydrogel could load antimicrobial agents and effectively suppress bacterial proliferation to protect the wound from bacterial invasion. These results suggest that the pullulan hydrogels prepared in this study may have high potential as new ideal wound-dressing materials. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
研究领域:
生物材料学
·
医学工程
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Applied Materials Today
·
2017
·
IF 0
Tao Li,Xiongbo Song,Changmei Weng,Xin Wang,Li Sun,Xiaoyuan Gong,Liu Yang,Cheng Chen
Injectable hydrogels derived from natural polysaccharides have great application prospects in cartilage tissue engineering (CTE). In this study, chondroitin sulfate was functionalized with adipic dihydrazide (CS-ADH), and pullulan was oxidized (oxPL). We fabricated a self-crosslink, biodegradable, in situ forming, injectable hydrogel by covalent hydrazone crosslinking of polysaccharides, without using extra cross-linking agents and releasing harmful byproducts under physiological conditions. The gelation time, equilibrium swelling, degradation behavior, network morphology and mechanical properties of CS-ADH/oxPL hydrogels were well manipulated by varying the weight ratio and concentration of CS-ADH and oxPL. In addition, encapsulation of rabbit articular chondrocytes in CS-ADH/oxPL hydrogels was observed after 14 days culture. Live/Dead staining assay demonstrated that the hydrogel system possessed good cytocompatibility. Sulfated glycosaminoglycan (sGAG) contents indicated that the hydrogel system favored cartilaginous extracellular matrix (ECM) deposition. Furthermore, the hydrogel system could maintain the chondrocyte phenotype and enhance chondrogenesis, which was evaluated in terms of their protein and gene expression. These features support that CS-ADH/oxPL hydrogels have a potential as an injectable cell delivery carrier scaffold in CTE.
研究领域:
生物材料
·
组织工程
·
生物医学工程
✓ 已验证
产品关联
DOI
International Journal of Bioprinting
·
2022
·
IF 7.422
Feng Zhaoxuan,Li Jinsong,Zhou Dasen,Song Hui,Lv Jiaqi,Bai Wenqin
Digital light processing has significant advantages, such as high repeatability, low failure rate, and no extrusion shear force. To make it possible to print complex structures with high resolution, the consecutive development of photocurable ink materials is indispensable. In this work, photo-functionalized pullulan (Pul-NB) was prepared by introducing norbornene groups into pullulan chains, and an ink material suitable for photocurable printing was prepared by thiol-ene click reaction. The rheology, water absorption, and mechanical properties of the Pul-NB precursor solution and photocurable hydrogel were investigated. The optimal composition of Pul-NB ink for three-dimensional (3D) printing was obtained by adjusting the degree of substitution, Pul-NB concentration, and thiol crosslinking agent. This novel bioink for digital light processing 3D printing showed good printability and high shape fidelity. This ink material provides an excellent alternative for printing biomimetic soft tissue organs, high-throughput tissue models, soft robots, etc.
研究领域:
生物打印
·
材料科学
·
组织工程
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2023
·
IF 5.8
Hao-Ying Li,En-Yu Xu
Two main challenges are associated with current spray-dried microparticles for inhalation, including the enhancement of aerosolization performance of microparticles and the creation of sustained drug release for continuous treatment on-site. For achieving these purposes, pullulan was explored as a novel excipient to prepare spray-dried inhalable microparticles (with salbutamol sulphate, SS, as a model drug), which were further modified by additives of leucine (Leu), ammonium bicarbonate (AB), ethanol and acetone. It was demonstrated that all pullulan-based spray-dried microparticles had improved flowability and enhanced aerosolization behavior, with the fine particle (<4.46µm) fraction of 42.0-68.7% w/w, much higher than 11.4% w/w of lactose-SS. Moreover, all modified microparticles showed augmented emitted fractions of 88.0-96.9% w/w, over 86.5% w/w of pullulan-SS. The pullulan-Leu-SS and pullulan-(AB)-SS microparticles demonstrated further increased fine particle (<1.66µm) doses of 54.7µg and 53.3µg respectively, surpassing that (49.6µg) of pullulan-SS, suggesting an additionally increased drug deposition in the deep lungs. Furthermore, pullulan-based microparticles revealed sustained drug release profiles with elongated time (60mins) over the control (2mins). Clearly, pullulan has a great potential to construct dual functional microparticles for inhalation with improved pulmonary delivery efficiency and sustained drug release on-site.
研究领域:
药学
·
药物递送系统
·
肺部药理学
·
制药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Zhaoxuan Feng,Shuangjia Cheng,Shuyu Chen,Jie Zhen,Wenqin Bai
3D-printable hydrogels confront two core bottlenecks: the trade-off among high printing resolution, fabrication efficiency, and structural fidelity, as well as the inherently poor mechanical performance of conventional hydrogel materials. Herein, we fabricated a dual-network photo-crosslinkable pullulan-based hydrogel system, in which the mass ratio of Pul-MA to uHMW Pul-NB and solid content jointly govern the printability and mechanical behaviors of hydrogel inks. A simple ethanol post-treatment induces relatively homogeneous volumetric shrinkage within printed architectures. Specifically, treatment with 75% ( v /v) ethanol shrinks the diameter of hydrogel micro-filaments down to 51.84 ± 0.4% of the original dimension, which greatly improves the feature resolution of printed structures. The ethanol-triggered shrinkage exhibits favourable partial reversibility, which holds promise for programmable dimensional regulation through further optimization of post-treatment cycles, accompanied by simultaneous mechanical reinforcement of hydrogel scaffolds. Complex architectures including bionic auricular models and co-axially printed tubular scaffolds were successfully fabricated to verify the practicability of this strategy. This solvent-induced shrinkage-assisted resolution-enhancing method serves as a hardware-free post-processing strategy for high-precision additive manufacturing of biomaterials, and holds practical value for research fields including tissue engineering, soft robotics and microfluidic devices.
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yuanyuan Bao,Dan Yang,Hui Liu,Shujing Li,Hong Meng
Preservative-free dry masks made from natural active ingredients have attracted much attention in the spirit of safety and environmental protection, which should be in line with the future trend of skin care products. Pterostilbene (PTS) is a natural antioxidant ingredient derived from plants that has received great interest in the field of biomedicine and cosmetics. However, the low water solubility and stability of PTS limiting its wide application. In this study, we first self-assembled Pterostilbene (PTS) and hydroxypropyl-β-cyclodextrin (HP-β-CD) into water-soluble inclusion complexes (IC) to solve the solubility problem of Pterostilbene. The dry mask (PUL-PTS-HP-β-CD-NF) was then successfully produced using electrostatic spinning technology via combining IC with pullulan polysaccharide as co-spinning agents. The PUL-PTS-HP-β-CD-NF masks were detailedly analyzed using SEM, FTIR, and XRD, and the antioxidant capacity, water retention capacity, in vitro release, and safety were comprehensively assessed at the same time. The results showed that the nanofiber mask (PUL-PTS-HP-β-CD-NF) was dense, uniform, and well-distributed, which exhibited a higher level of antioxidant activity compared to isolated PTS counterparts. As the IC increases, the water retention capacity of the PUL-PTS-HP-β-CD-NF mask becomes more evident, and the in vitro dissolving studies further demonstrated that the release of PTS from PUL-PTS-HP-β-CD-NF was concentration-dependent, which exhibited a direct relationship with time and followed a kinetic model of first-order reaction. In addition, the biosafety of the nanofiber mask was confirmed using red blood cell hemolysis. This work not only provide a very novel approach to prepare dry mask but also offer a sustainable way to create environmentally-friendly chemicals.
研究领域:
化妆品科学
·
材料科学
·
生物医药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2024
·Vol. 89
·期 11
·7803-7818
·
IF 3.2
Jianfeng Zhou,Weiqiang Wang,Xingjian Yang,Deng-Guang Yu,Ping Liu
Bio-based active food packaging materials have a high market demand. We use coaxial electrospinning technology to prepare core–shell structured nanofibers with sustained antibacterial and antioxidant properties. The fiber core layer was composed of gelatin and tea polyphenols, whereas tea polyphenols provide antibacterial and antioxidant properties; the fiber sheath was composed of pullulan polysaccharides with antioxidant properties. By using a scanning electron microscope, it can be seen that the diameter distribution of the prepared nanofibers was uniform and the surface is smooth; using a transmission electron microscope, it can be clearly seen that the nanofibers have a core–shell structure; Fourier Transform Infrared and X-ray diffraction analysis indicate that the nanofibers have an amorphous structure; the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging shows that nanofibers have higher antioxidant properties with the addition of tea polyphenols; antibacterial test showed that nanofibers had obvious inhibitory effect on the growth of Staphylococcus aureus and Escherichia coli ; and the nanofiber film dissolution test shows that nanofibers can be used as fast soluble active packaging. Finally, core–sheath-structured nanofibers can serve as active packaging for instant food, possessing both rapid water solubility and excellent antibacterial and antioxidant activity, making water-soluble nanofibers interesting applications in the field of food packaging.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Baoshan Tang,Xi Wu,Lanxiang Liu,Juan Xu,Jinju Ma,Hong Zhang
In this study, multifunctional green carbon dots (CDs) have been synthesized using Galla chinensis waste (GCW) via hydrothermal method for the first time. An active packaging film has been developed in this work by combining CDs and pullulan (PL), using the solution-casting method. The microscopic morphology revealed that the CDs that were prepared using GCW exhibited good compatibility with PL. In addition, it also led to improvement in the toughness of the PL film (14.01 % to 20.26 %), along with its water vapor permeability value [1.31 to 0.53 (g·mm)/(kPa·h·m 2 )]. The composite films consisting of CDs exhibited good UV blocking rates for the UVA (90.41 %–7.87 %), UVB (87.76 %–0.08 %), and UVC (83.39 %–0 %) spectral ranges. The composite films exhibited strong antioxidant activity , and the clearance of ABTS and DPPH were obtained to be 93.61 % and 86.30 %, respectively. In addition, the composite films showed good antibacterial activity for E. coli and S. aureus , with a high antibacterial rate of up to 99.99 %. Finally, the non-contact preservation of strawberries over a duration of 10 d at room temperature confirmed that the prepared composite film can help preserve the quality of strawberries, as well as extended their shelf-life.
研究领域:
食品科学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）