OPTICAL MATERIALS
·
2025
·
IF 4.2
Wei Tan,Fengdi Fang,Xingbao Han,Lan Cheng,Pengli Zuo
As a new kind of fluorescent nanomaterial, carbon dots (CDs) have gained considerable focuses in regulating and improving their intrinsic properties. Some effective methods have been proposed and validated, like surface passivation and heteroatom doping in CDs. Herein, we have facilely fabricated the fluorescent nitrogen, silicon co-doped carbon dots (N,Si-CDs) by a one-pot solvothermal approach. N-[3-(Trimethoxysilyl)propyl]ethylenediamine and citrate were adopted as precursors for nitrogen, silicon, and carbon sources, respectively. Correspondingly, the formation of N,Si-CDs was affirmed via TEM, FT-IR, and XPS techniques. Further analysis of the optical properties was conducted using UV-vis spectrophotometry and fluorescence spectroscopy. Lastly, the prepared N,Si-CDs were utilized to investigate their sensing ability for rifampicin and Cr(Ⅵ) as well as photothermal effect. The detection linear range of this sensing probe for rifampicin was 0–18 μM, achieving the detection limit of 0.02 μM, besides the detection linear range for Cr(Ⅵ) was 0–130 μM, reaching the detection limit of 0.15 μM. Furthermore, the photothermal conversion efficiency (PCE) of N,Si-CDs was explored and calculated to be 43.45% with a favorable photothermal effect. Collectively, this research will provide novel insights for fluorescence sensing strategies and explore new proposals to develop CDs-based photothermal agents (PTAs) for cancer photothermal therapy (PTT).
✓ 已验证
产品关联
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 3.9
Chang Liu,Xiaoyue Zhang,Yu Zhou,Lin Zhu,Chengyu Zhang,Xiaohui Yan,Shengping You,Wei Qi,Rongxin Su
The enzyme leakage in physical adsorption dramatically limits the application of immobilized laccase (Lac) in environmental treatment. Therefore, a leakage-proof Zr-based metal-organic framework (MOF) carrier, UiO-66-NH 2 (30), was constructed for the stable loading of Lac and efficient antibiotic biodegradation, which could fix the Lac inside the carrier rather than only adsorbed on the surface. Based on the aperture modulation strategy, the pore size distribution of MOFs at about 10 nm was significantly increased to match the diameter of Lac (7.6 nm), by which Lac@UiO-66-NH 2 (30) was successfully prepared with a high enzyme loading amount (275.96 mg/g) and high enzyme recovery (618 %). The immobilized Lac@UiO-66-NH 2 (30) could nearly 100 % biodegrade rifampicin and lincomycin in 6 min and 6 h, respectively, and after 8 times reuse, the degradation rates of antibiotics remained at 81.46 % and 76.12 %, which showed excellent reusability and stability. Overall, Lac@UiO-66-NH 2 (30) is expected to be an ideal candidate for pollution control.
✓ 已验证
产品关联
DOI
高影响因子
MONATSHEFTE FUR CHEMIE
·
2024
·
IF 1.7
Qiu Shengzhou,Wu Chenxi,Akter Sima,Hong Shaoli,Liu Huihong,Mahmud Sakil
Addressing the challenges of tuberculosis drug detection, this study investigates the application of copper(II) oxide modified screen-printed carbon electrodes (CuO/SPCEs) for the electrochemical detection of rifampicin, a critical drug in the treatment of tuberculosis and mycobacterial infections. The CuO/SPCE was fabricated via a constant potential electrodeposition technique and characterized using voltammetry. The bare SPCE surface exhibited a rough, porous structure, whereas the electrodeposition of CuO resulted in a smoother CuO/SPCE surface. The sensor exhibited high sensitivity with a detection limit of 2.89 μM for rifampicin and demonstrated excellent stability with a relative standard deviation of 3.5% over 10 measurements. The reproducibility was confirmed with an RSD of 4.2% across different electrodes. It also showed a linear response to rifampicin concentrations ranging from 10 to 200 μM. The real sample analysis showed a linear relationship between rifampicin concentration and peak current ( I = 0.739 + 0.008 c ), with a high correlation coefficient ( R 2 = 0.995). This electrochemical sensor’s simplicity, cost-effectiveness, and rapid response make it a promising tool for point-of-care applications in tuberculosis management. Graphical abstract
✓ 已验证
产品关联
DOI
高影响因子
WATER RESEARCH
·
2023
·
IF 12.8
Jiayi Wu,Congcong Liu,Rui Wang,Saitao Yan,Baoliang Chen,Xiaoying Zhu
The microbial biofilm formed on plastics, is ubiquitous in the environment. However, the effects of antibiotic resistance on the development of the biofilm on plastics, especially with regard to initial cell attachment, remain unclear. In this study, we investigated the initial bacterial adhesion and subsequent biofilm growth of a rifampin (Rif) resistant E. coli (RRE) and a normal gram-positive B. subtilis on a typical plastic (polyethylene, PE). The experiments were conducted in different antibiotic solutions, including Rif, sulfamethoxazole (SMX), and kanamycin (KM), with concentrations ranging from 1 to 1000 μg/L to simulate different aquatic environments. The AFM-based single-cell adhesion force determination revealed that Rif resistance strengthened the adhesion force of RRE to PE in the environment rich in Rif rather than SMX and KM. The enhanced adhesion force may be due to the higher secretion of extracellular polymeric substances (EPS), particularly proteins, by RRE in the presence of Rif compared to the other two antibiotics. In addition, the higher ATP level of RRE would facilitate the initial adhesion and subsequent biofilm growth. Transcriptome analysis of RRE separately cultured in Rif and SMX environments demonstrated a clear correlation between the expression of Rif resistance and the augmented bacterial adhesion and cellular activity. Biofilm biomass analysis confirmed the promotion effect of Rif resistance on biofilm growth when compared to non-resistant biofilms, establishing a novel association with the augmentation of microbial adhesion force. Our study highlights concerns related to the dissemination of antibiotic resistance during microbial colonization on plastic that may arise from antibiotic resistance.
✓ 已验证
DOI
PubMed
高影响因子
POLYHEDRON
·
2026
·
IF 2.8
Nan Wang,Lei Chen,Wanhe Du,Keying Zhu,Bingyu Zhang,Shengwen Chen,Zhiguo Sun,Jifen Wang
A novel Z -scheme CuWO 4 /Cu/Cu 2 O ternary heterojunction photocatalyst was successfully synthesized via a solvothermal approach, featuring the low-cost metallic copper (Cu) as an efficient electron mediator. This unique structure was designed to enhance Cu 2 O visible light capture capability and accelerate photogenerated charge separation, thereby efficiently degrading the recalcitrant antibiotic Rifampicin (RFP). Comprehensive characterizations (XRD, XPS, SEM, EDS) confirmed the successful construction of the heterojunction, where CuWO 4 and Cu particles are integrated onto the surface of the Cu 2 O. Compared to individual components and binary composites, the optimized CuWO 4 /Cu/Cu 2 O sample demonstrated significantly superior photocatalytic activity, achieving an impressive 88.0% RFP degradation rate within 80 min under visible light. The enhanced performance is primarily ascribed to the successful formation of a Z -scheme charge transfer pathway, where the localized surface plasmon resonance (LSPR) effect of the metallic Cu mediator promotes carrier migration, thereby improving both carrier separation efficiency and redox capacity. Kinetic analysis confirmed a degradation rate constant ( k ) of 0.02743 min −1 , approximately 2.8 times higher than that of pure Cu 2 O. Furthermore, the composite exhibited excellent stability, maintaining a degradation rate above 80% after five recycling runs and demonstrating robust performance across a broad pH range (3.0–7.0). Based on active species trapping and band structure analysis, a Z -scheme mechanism was proposed, highlighting O 2 − as the dominant active species. This study provides a facile and low-cost strategy for constructing highly efficient LSPR-enhanced Z -scheme photocatalysts for environmental remediation.
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 31
·期 35
·2103442
·
IF 18.808
Shuang Wu,Yi Huang,Jiachang Yan,Yuzhen Li,Jinfeng Wang,Yi Yan Yang,Peiyan Yuan,Xin Ding
The efficacy of conventional antibiotics therapeutics has declined rapidly due to the emerged antibiotic resistance. There is an urgent need to develop novel approaches to address the problem of antibiotic shortage, particularly for Gram-negative bacteria. Herein, a biomimetic nanodelivery system is proposed to enhance the bacterial targeting and uptake of rifampicin (Rif), a traditional antibiotic but not effective against Gram-negative bacteria. The biomimetic nanodelivery system (Rif@MSN@OMV) is composed of outer membrane vesicles (OMVs) isolated from E. coli as shell and rifampicin-loaded mesoporous silica nanoparticles (MSNs) as core. The OMVs greatly improve the uptake of MSNs in E. coli , but not in Gram-positive bacteria S. aureus , owing to the homotypic targeting function of the OMVs. The Rif@MSN@OMV exhibits enhanced antimicrobial activity against E. coli and completely eradicates bacteria at an equivalent rifampicin concentration (4 µg mL −1 ) while free rifampicin shows weak bactericidal activity. Meanwhile, the Rif@MSN@OMV maintains good biocompatibility both in vitro and in vivo. More importantly, the Rif@MSN@OMV elevates survival rate of infected mice and reduces bacterial load in intraperitoneal fluid and organs. Overall, the OMVs-coated nanodelivery system provides a novel strategy to improve the antimicrobial efficacy of conventional antibiotic or repurpose drugs for treatment of Gram-negative bacterial infections.
✓ 已验证
产品关联
DOI
高影响因子
MICROBIAL PATHOGENESIS
·
2026
·
IF 3.9
Guangyuan Jia,Qian Dong,Zhe Song,Baoqing Zhang
In the current scenario, Klebsiella pneumoniae -induced pediatric lung inflammation is a major health issue, made worse by multidrug-resistant infection. Mainly, Carbapenem-resistant K. pneumoniae (CR- K.pneu ) infections are rising due to antimicrobial resistance, highlighting the urgent need for effective treatments. Additionally, lung inflammation can complicate the treatment of lung cancer, as it may hinder therapeutic responses. Herein, the study aimed to explore the adipose mesenchymal stem cells derived exosomal vesicles (ADMSCs-EVs) loaded with rifampcin (Rf) against CR- K. pneu infection in mice model. ADMSCs-EVs were extracted by the ultracentrifugation method and characterized via UV-visible spectra, FTIR, TEM, DLS and Zeta potential. It exhibited strong antioxidant potential confirmed via DPPH assays. The antibacterial assay was significantly inhibited at 100 μg/ml of ADMSCs-EVs-Rf against CR- K.pneu . Light microscopy displays biofilm eradication at the doses of 75 and 100 μg/ml. In vitro biocompatibility of ADMSCs-EVs-Rf enhances proliferation, suppresses oxidative stress, accelerates wound healing, and significantly attenuates inflammatory cytokine production in LPS-induced pediatric NHBE cells. Further, the in vivo antibacterial activity of ADMSCs-EVs-Rf was examined in a CR- K.pneu infection mice model. The treatment significantly decreased MDA levels while increasing the antioxidant markers GSH, SOD, and CAT (p < 0.05), indicating attenuation of oxidative stress. The biocompatibility of ADMSCs-EVs-Rf showed low toxicity at 5 mg/ml. ADMSCs-EVs-Rf may be useful for treating inflammation, fixing damaged cells, clearing out alveolar fluid, and getting rid of microbes. Hence, ADMSCs-EVs-Rf might be a promising option for treating CR- K. pneu infections, particularly those affecting children's respiratory systems and lung cancer.
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Junyuan Shi,Jun Wu,Yunkai Zhang,Jingqi Wang,Siting Peng,Wendi Xiao,Yafei Hou,Da Chen
Dual-excitation N, Cl-CDs are synthesized for ratiometric RIF sensing. • Ratiometric detection of RIF is achieved with a detection limit of 58 nM. • The sensing mechanism involves synergistic IFE and FRET. • A smartphone integrated finger-actuated microfluidic device enables on-site visual RIF detection. • The platform exhibits satisfactory recovery in aquatic product samples. The increasing environmental prevalence of the antibiotic rifampicin (RIF) poses potential hazards to public health and ecological stability. Herein, a novel ratiometric assay is established by utilizing the dual-excitation nitrogen and chlorine co-doped carbon dots (N, Cl-CDs) for the quantification of trace amounts of RIF. Benefiting from the synergistic effects of the inner filter effect and Förster resonance energy transfer, the N, Cl-CDs act as a sensitive nanoprobe for RIF detection over a concentration range of 0.058–35 μM, with a detection limit of 58 nM. Furthermore, a novel finger-actuated microfluidic sensing platform has been developed through the integration of N, Cl-CDs. By combining the dual-excitation property of the probe with a smartphone-based RGB readout, the platform achieves on-site and visual quantification of RIF in real samples, as validated by high recovery rates ranging from 98.8% to 101.0%. Therefore, this work offers an innovative strategy for RIF sensing and establishes a versatile platform boasting prominent prospects for on-site food safety monitoring. Download: Download high-res image (150KB) Download: Download full-size image
✓ 已验证
产品关联
DOI
高影响因子
NEUROTOXICOLOGY
·
2022
·
IF 4.398
Xiaoyu Qu,Jie Ma,Huan Gao,Yueming Zhang,Jinghui Zhai,Jiawei Gong,Yanqing Song,Tingting Hu
Isoniazid (INH) and rifampicin (RIF) are co-administered in tuberculosis treatment but can cause neurotoxicity , and the mechanism is not known. To explore this mechanism, we employed an integrated approach using metabolomics analysis (MA) and proteomics analysis (PA). Male mice were divided into three groups and administered vehicle (control group), or co-administered INH (120 mg/kg) and RIF (240 mg/kg), for 7 or 14 days. Mice brains were collected for mass spectrometry-based PA and MA plus lipidomics analysis. Measurement of brain levels of malondialdehyde and superoxide dismutase revealed time-dependent brain injury after exposure to INH+RIF for 7 and 14 days. Also, 422 proteins, 35 metabolites, and 21 lipids were dysregulated and identified. MA demonstrated “purine metabolism,” “phenylalanine, tyrosine and tryptophan biosynthesis,” “biosynthesis of unsaturated fatty acids ,” “phenylalanine metabolism,” and “arginine biosynthesis” to be disturbed significantly. PA demonstrated pathways such as “lipids,” “amino acids,” and “energy metabolism” to be disrupted. Peroxisome proliferator-activated receptor (PPAR) pathways were changed in energy metabolism, which led to the neurotoxicity induced by INH+RIF. Immunohistochemical analyses of PPARs in mice brains verified that PPAR-α and -γ expression was downregulated. PPAR-α and -γ activation might be a key target for alleviating INH+RIF-induced neurotoxicity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Zheng-Li Hu,Jiu-Jiu Di,Hao-Yu Lian,Xianliang Yi,Hao Zhou,Yang Liu
Pathway design for reactive oxygen species (ROS) production has raised great interest for advanced oxidation in water treatment. Herein, the surface oxygen vacancies (OVs) on ramsdellite-type MnO 2 were modified via atmospheric-pressure DBD plasma. By simply submitting the MnO 2 powder to N 2 discharge zone, the abundance and reactivity of OVs were improved at a low processing energy (input power 14 W) and after moderate processing time of 20 min (N14-20). The structures were characterized with XRD, FTIR, SEM, (HR)TEM, XPS and CO-TPR. The activities for rifampicin oxidation were evaluated without light illumination, thermal activation or any additional oxidant. The phase composition and surface functional groups were not changed after DBD-N 2 -plasma treatment, but the original big nanorods were collapsed into small flakes. Compared with •OH and 1 O 2 , O 2 •- served as primary ROS for rifampicin degradation. In this process, the dissolved oxygen (DO) in water was the precursor of ROS, and the larger amount of surface OVs on N14-20 remarkably facilitated DO activation into O 2 •- , perfectly eliminating rifampicin ( e.g. , removal for 60 mg/L rifampicin by 0.003 g catalyst was ∼ 70 % for N14-20 and ∼ 60 % for virgin MnO 2 ; removal for 100 mg/L rifampicin by 0.01 g catalyst was 68 % and 53 %, respectively).
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 18
·13812
·
IF 5.6
Miran Tang,Deyi Zhao,Sichen Liu,Xiaotuan Zhang,Zhuocheng Yao,Hule Chen,Cui Zhou,Tieli Zhou,Chunquan Xu
Combining pentamidine with Gram-positive-targeting antibiotics has been proven to be a promising strategy for treating infections from Gram-negative bacteria (GNB). However, which antibiotics pentamidine can and cannot synergize with and the reasons for the differences are unclear. This study aimed to identify the possible mechanisms for the differences in the synergy of pentamidine with rifampicin, linezolid, tetracycline, erythromycin, and vancomycin against GNB. Checkerboard assays were used to detect the synergy of pentamidine and the different antibiotics. To determine the mechanism of pentamidine, fluorescent labeling assays were used to measure membrane permeability, membrane potential, efflux pump activity, and reactive oxygen species (ROS); the LPS neutralization assay was used to evaluate the target site; and quantitative PCR was used to measure changes in efflux pump gene expression. Our results revealed that pentamidine strongly synergized with rifampicin, linezolid, and tetracycline and moderately synergized with erythromycin, but did not synergize with vancomycin againstE. coli,K. pneumoniae,E. cloacae, andA. baumannii. Pentamidine increased the outer membrane permeability but did not demolish the outer and inner membranes, which exclusively permits the passage of hydrophobic, small-molecule antibiotics while hindering the entry of hydrophilic, large-molecule vancomycin. It dissipated the membrane proton motive force and inactivated the efflux pump, allowing the intracellular accumulation of antimicrobials that function as substrates of the efflux pump, such as linezolid. These processes resulted in metabolic perturbation and ROS production which ultimately was able to destroy the bacteria. These mechanisms of action of pentamidine on GNB indicate that it is prone to potentiating hydrophobic, small-molecule antibiotics, such as rifampicin, linezolid, and tetracycline, but not hydrophilic, large-molecule antibiotics like vancomycin against GNB. Collectively, our results highlight the importance of the physicochemical properties of antibiotics and the specific mechanisms of action of pentamidine for the synergy of pentamidine–antibiotic combinations. Pentamidine engages in various pathways in its interactions with GNB, but these mechanisms determine its specific synergistic effects with certain antibiotics against GNB. Pentamidine is a promising adjuvant, and we can optimize drug compatibility by considering its functional mechanisms.Keywords:gram-negative bacteria;pentamidine;linezolid;synergy;adjuvant;efflux pump;outer membrane;vancomycin
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 29
·3594-3601
·
IF 2.073
Qing Huang,Xiaokun Li,Suxiang Feng,Wenfeng Zhuge,Fengping Liu,Jinyun Peng,Shancai Mo
The composite of molybdenum carbides and multiwalled carbon nanotube (MWCNTs–Mo2C) modified glassy carbon electrode (GCE) was developed to prepare an electrochemical sensor for the ultrasensitive detection of rifampicin. Solvothermal and annealing methods were used to synthesize the MWCNTs–Mo2C composite. The morphology and size of the synthesized materials were characterized using a scanning electron microscope and atomic force microscope. The MWCNTs–Mo2C composite shows a large effective surface area and reveals high electron transfer rate, indicating its excellent electrocatalytic activity. Under optimal conditions, the modified electrode used to analyze rifampicin shows an excellent linear relationship between the peak current and the rifampicin concentration in the range from 0.5 to 74 μM. The limit of detection was 0.045 μM (S/N = 3). The sensor was successfully applied to actual drugs and human serum testing with satisfactory results.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）