Journal of Materials Chemistry B
·
2015
·Vol. 3
·期 13
·2685-2697
·
IF 4.726
Xinyu Hu,Wei Wei,Xiaoliang Qi,Hao Yu,Liandong Feng,Junjian Li,Shiming Wang,Jianfa Zhang,Wei Dong
Salecan is a novel water-soluble extracellular β-glucan produced by a salt-tolerant strain Agrobacterium sp. ZX09. Salecan is suitable for the fabrication of hydrogels for biomedical applications due to its excellent physicochemical and biological properties. In this paper, a series of pH-sensitive hydrogels were prepared in aqueous solution by the graft copolymerization of Salecan and acrylic acid (AA) using N,N′-methylene diacrylamide as a crosslinker for controlled drug delivery. The structure and thermal stability of the resulting hydrogels were characterized by FT-IR, XRD and TGA. By SEM analysis, freeze-dried hydrogels displayed an interconnected porous structure with tunable pore size in the range of 23.2–90.3 μm. The swelling behavior of the hydrogels was shown to be highly dependent on the environmental pH, salt type and concentration, as well as the contents of Salecan and BAAm. They are almost unswellable at pH 1.2 and swollen extensively at pH 6.86. Meanwhile, the increase in the content of hydrophilic Salecan could enhance the swelling ratio, whereas the presence of more BAAm reduced the swelling capacity but promoted the water retention to some extent. Rheological tests revealed that storage modulus G′ was strongly influenced by the crosslink density of the obtained hydrogel network. Especially, doxorubicin (DOX) as a model anti-cancer drug was very efficiently loaded into the negatively charged hydrogels (up to 69.4 wt%) through electrostatic interactions. More importantly, the release of DOX from this intelligent system exhibited pH-responsive behavior and a sustained release pattern. For SPA2, the cumulative release profile showed a low level of drug release (about 12.3 wt% in 24 h) at pH 7.4, and was significantly accelerated at pH 4.0 (over 40 wt% in 6 h). Cytotoxicity experiments confirmed that all blank hydrogels were non-toxic to A549 cells, while DOX released from the drug-loaded hydrogels remained biologically active and had the capability to kill cancer cells. The preliminary results clearly suggested that the Salecan-g-PAA hydrogels may be promising carriers for controlled drug delivery.
研究领域:
生物医学工程
·
药学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2017
·
IF 3.671
Xinyu Hu,Yongmei Wang,Liangliang Zhang,Man Xu,Jianfa Zhang,Wei Dong
Salecan, a novel water-soluble extracellular β-glucan, is very suitable for the hydrogel preparation. Here, pH-sensitive magnetic composite hydrogel was fabricated by the graft copolymerization of crotonic acid (CA) and N-(hydroxymethyl) acrylamide (HMAA) onto salecan in the presence of Fe 3 O 4 @SiO 2 nanoparticles for doxorubicin (DOX) delivery. The embedment of Fe 3 O 4 @SiO 2 nanoparticles into salecan-g-poly(CA-co-HMAA) hydrogel network endowed the material with magnetic property. In addition, DOX not only achieved effectively bound to the composite hydrogel, but also released in a controlled and pH-dependent manner. The application of an external magnetic field could significantly enhance the drug release rate. More importantly, the released DOX preserved its bioavailability. Taken together, these hydrogel drug carriers provide a promising platform for magnetically targeted drug delivery.
研究领域:
材料科学
·
药学
·
纳米技术
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2014
·
IF 3.916
Xinyu Hu,Liandong Feng,Wei Wei,Aming Xie,Shiming Wang,Jianfa Zhang,Wei Dong
Salecan is a novel water-soluble, high molecular mass extracellular β-glucan produced by Agrobacterium sp. ZX09. Salecan has excellent physicochemical and biological properties, making it very suitable for hydrogel preparation. In this study, a series of novel semi-interpenetrating polymer network (semi-IPN) hydrogels containing Salecan and poly(N,N-dimethylacrylamide-co-2-hydroxyethylmethacrylate) (poly(DMAA-co-HEMA)) were synthesized by radical polymerization and semi-IPN technology. Structure and morphology of the hydrogels were characterized by FTIR, XRD, TGA and SEM. The semi-IPNs had a well-interconnected porous structure with tunable pore size ranging from 6 to 41 μm. Swelling capability of the hydrogels was improved by introducing the hydrophilic Salecan. Rheological results indicated that the incorporation of poly(DMAA-co-HEMA) into hydrogels enhanced the storage modulus. Compression tests revealed that these semi-IPNs were robust materials with compressive modulus between 13.3 and 90.5 kPa, the addition of Salecan increased the fracture strain from 71.1% to 88.8%. Degradation and cytotoxicity tests demonstrated that semi-IPNs were degradable and non-toxic.
研究领域:
材料科学
·
高分子化学
·
生物医学工程
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2017
·
IF 3.671
Xinyu Hu,Yongmei Wang,Liangliang Zhang,Man Xu,Jianfa Zhang,Wei Dong
Salecan is a novel water-soluble extracellular β-glucan and suitable for the hydrogel preparation due to its excellent physicochemical and biological properties. The present article describes the fabrication and characterization of a pH/magnetic field-driven hydrogel based on salecan-g-poly(vinylacetic acid-co-2-hydroxyethyl acrylate) [poly(VA-co-HEA)] copolymer and Fe 3 O 4 @Agarose nanoparticles for drug release testing. Vibrating sample magnetometer characterization verified that integration of Fe 3 O 4 @Agarose nanoparticles in the copolymer provided the sensitivity to magnetic fields. The doxorubicin hydrochloride (DOX) release test showed a pH/magnetic field-triggered and sustained release property, and the release could be accelerated under mildly acidic conditions or the presence of an external magnetic field. Meanwhile, the increase in salecan content could also enhance the release rate. Cytotoxicity assays revealed that the released DOX maintained relatively high killing efficacy of A549 cells. In sum, these salecan-g-poly(VA-co-HEA)/Fe 3 O 4 @Agarose hydrogels were well-suited for magnetically targeted drug delivery systems.
研究领域:
药学
·
材料科学
·
纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Xinyu Hu,Yongmei Wang,Man Xu
Salecan is a linear microbial polysaccharide obtained from the fermentation of Agrobacterium sp. ZX09. Salecan is suitable for the preparation of hydrogels for biomedical applications, given its prominent physicochemical and biological properties. Herein, we described a novel microwave-assisted polymerized composite hydrogel that was comprised of salecan/poly(diacetone acrylamide) (PDAAm) network and tantalum carbide (TaC) nanoparticles. The incorporation of TaC nanoparticles into the composite hydrogels was confirmed by XRD and EDX analysis, and can be engineered as means for structural reinforcement, thus leading to a remarkable improvement in their thermal stability and mechanical properties. Compared to bare salecan/PDAAm hydrogel, >9.3-fold increase in compressive modulus and 7-fold increase in storage modulus were achieved with the addition of 3 mL of TaC nanoparticles dispersion (5%, w/v). More importantly, cytotoxicity results revealed that the composite hydrogels were non-toxic to L929 and 3T3-L1 cells. Cell proliferation and Live/Dead staining demonstrated that salecan/PDAAm/TaC composite hydrogels provided a desired environment for cell adhesion, and allowed proliferation at the normal rate. All the results highlight the value of this system for potential application as cell adhesion material.
研究领域:
材料科学
·
生物医学工程
·
纳米技术
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）