Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Bingbing Xu,Zishan Hou,Zaixing Li,Wenjing Zhang,Xue Qin,Yi Xing,Chen Hong,Kai Wang,Nannan Wang
Antibiotic residue is a hazardous waste which contains much protein; the extraction of protein from antibiotic residue using neutral protease is an effective utilization path of antibiotic residue. In this work, an immobilized magnetic neutral protease (IMNP) was successfully prepared by immobilizing free neutral protease on the surface of magnetic carrier, and characterized by a series of analysis methods (SEM, BET, FTIR, TGA , XRD, and VSM). IMNP can retain > 90 % enzyme activity of free neutral protease and extract ∼36 % protein from streptomycin residue within 4 h at the optimal extraction condition (water content in antibiotic residue = 97.5 %, IMNP loading = 5 %, pH = 7.0, and extraction temperature = 50 ℃). The loss of neutral protease from the surface of IMNP and the possible poisoning of IMNP caused by the toxic substances in the streptomycin residue significantly affected the relative enzyme activity of IMNP (29.4 % enzyme activity of IMNP lost after 6th use). IMNP showed better storage performance, pH and thermal tolerance performance than those of free neutral protease.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Yuanling Sun,Rui Han,Yuxue Dai,Xiaodong Zhu,Hao Liu,Dandan Gao,Chuannan Luo,Xueying Wang,Qin Wei
In our work, a highly selective and sensitive streptomycin (STR) chemiluminescence (CL) sensor was successfully prepared based on aptamer and G-quadruplex DNAzyme (G-DNAzyme) modified three-dimensional graphene composite. Initially, β-cyclodextrins and ionic liquids functionalized graphene oxide aerogel (β-CD/ [email protected] ) was successfully prepared and characterized, in which graphene oxide aerogel (GOGA) was used as a skeleton material and provided large specific surface area, β-CD rich in hydroxyl groups and IL containing lots of stable ions increased the biocompatibility and stability of the composite, respectively. Then, tetracycline aptamer (S-Apt) and G-DNAzyme was immobilized on the surface of β-CD/ [email protected] , consecutively. Finally, G-DNAzyme/S-Apt/β-CD/ [email protected] was used to construct the CL sensor for STR detection. S-Apt with specific recognition ability to its target and G-DNAzyme as a catalyst of luminol-H 2 O 2 CL system improved the selectivity and sensitivity of the sensor, respectively. When STR existed, G-DNAzyme was released from the surface of S-Apt/β-CD/ [email protected] due to the specific recognition and binding ability between STR and S-Apt, catalyzing the CL reaction. The CL sensor showed the linear range of 1.4 × 10 −12 to 2.8 × 10 −9 mol/L and detection limit of 9.2 × 10 -14 mol/L (3 δ ). It was successfully used for STR detection in cucumber and milk samples.
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Journal of Chemistry
·
2025
·Vol. 2025
·期 1
·7819395
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IF 2.6
Siying Ma,Bingqian Weng,Yingying Huang,Shaoyun Chen
A rapid, simple, and stable detection method for streptomycin has been established based on the coloration reaction of 3,3′,5,5′-tetramethylbenzidine (TMB) catalyzed by peroxidase-mimicking DNAzymes (PMDs). It is discovered that the catalytic activity of PMDs constructed with G-quadruplex and hemin is weakened in the presence of streptomycin. In the presence of streptomycin, the OD 450nm value of the TMB colorimetric sample catalyzed by PMDs is significantly lower than that of the control sample (without streptomycin). Hence, the detection of streptomycin can be accomplished by detecting the change of OD 450nm (△OD 450nm ). Furthermore, when the concentration of the substrate TMB is lower, the catalytic activity of PMDs is inhibited more significantly by streptomycin. Under optimized conditions, this method has good detection specificity, could effectively distinguish streptomycin from other antibiotics (ampicillin, chloramphenicol, geneticin, and kanamycin), has a limit of quantitation (LOQ) of 5 mg/L, excellent linear performance, good repeatability, and a total detection time of less than 2 h, and can be employed for the detection of actual samples (laver and egg drop soup). This method circumvents the complex preparation process of experimental materials, is simple to operate, does not require fluorescent labeling, and is of low cost, providing a novel method for food safety supervision.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Zhaozhou Li,Xuebing Wei,Lijuan Wang,Ran Zhang,Huawei Niu,Jian Tao,Yafang Xie,Lele Li,Yajuan Li,Xiujin Chen,Yao Wang,Hongli Gao,Huichun Yu,Yunxia Yuan,Fang Li,Ningbo Wan,Daomin Li
An innovative streptomycin-specific electrochemical sensor was successfully established using a dual-recognition model based on the integration of molecular imprinting technology with aptamer affinity recognition. The functional interface was fabricated with a MXene/AuNPs composite covered by the specific aptamer and electropolymerized dopamine-imprinted film. Under the optimized conditions, the electrochemical sensor demonstrated a wide linear range from 1.0 × 10 −12 mol/L to 1.0 × 10 −4 mol/L with a detection limit of 0.0316 pmol/L for streptomycin. With the developed sensor, specific, rapid and accurate monitoring of streptomycin residues in milk samples was successfully achieved. The recoveries varied from 88.0 % to 105.8 % with relative standard deviations ranging from 1.7 % to 2.3 %. Overall, with the remarkable performance, the integration of MIPs with aptamer offers a novel approach to prepare the dual-recognition sensing interface and exhibits extensive adaptability and broad application prospects for the measurement, inspection and control of hazardous chemical substances in the complex matrices.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
XianZhu Meng,HuiWen Gu,HongChao Yi,YongQiang He,Ying Chen,WeiYin Sun
A MOF-based bio-bar code material was synthesized and firstly applied to develop an electrochemical streptomycin (STR) aptasensor . By using MOF-based bio-bar code and enzyme-assisted target recycling for dual-signal amplification, highly sensitive detection of STR was achieved. The sensing surface was simply fabricated by immobilizing a mixed monolayer of thiolated cDNA/aptamer duplexes (dsDNA) and 6-mercapto-1-hexanol (MCH) on the gold nanoparticle modified screen printed carbon electrode (Au/SPCE). The presence of target STR caused highly efficient removal of the aptamers from dsDNA assisted by Exo I enzyme . Then MOF-based bio-bar codes were backfilled to achieve the adsorption of electroactive Ru(NH 3 ) 6 3+ (RuHex) on electrode surface. The electrochemical signal of the surface-confined RuHex was used for quantitation. The analytical performance for STR was satisfactory with a wide linear range of 0.005–150 ng mL −1 , a low detection limit of 2.6 pg mL −1 and a good selectivity towards other three antibiotics. Moreover, the application of this aptasensor for determination of STR in real milk samples was also realized. With these merits, this dual-signal amplification assay might provide one of the effective ways for food safety monitoring.
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RSC Advances
·
2016
·Vol. 6
·期 45
·39401-39408
·
IF 3.289
Yan Yin,Xiaoli Qin,Qianchao Wang,Yin Yin
A facile electrochemical aptasensor based on a novel signal amplification strategy was employed for sensitive and selective detection of streptomycin for the first time. The aptasensor was prepared by sequentially dripping a gold nanoparticle-functionalized magnetic multi-walled carbon nanotube (Au@MWCNTs–Fe3O4) composite and nanoporous PtTi (NP-PtTi) alloy onto a glassy carbon electrode (GCE) surface. The NP-PtTi alloy with a uniform nanoporous structure was successfully fabricated by selectively de-alloying Al from a PtTiAl alloy in HCl solution. The results demonstrated that the Au@MWCNTs–Fe3O4/NP-PtTi composite not only showed a remarkable synergistic effect towards the aptasensor, but also provided a beneficial immobilization platform for the streptomycin aptamer carrier. Under optimal conditions, a wide linear range from 0.05 to 100 ng mL−1 and a detection limit of 7.8 pg mL−1 were obtained. The designed aptasensor showed an excellent analytical performance with good reproducibility, high selectivity and stability. Finally, the proposed aptasensor was successfully used for the detection of streptomycin in a real sample.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Junling Yin,Wenjuan Guo,Xiaoli Qin,Juan Zhao,Meishan Pei,Feng Ding
Quantitative detection of antibiotic residues in animal food stuffs is of great significance. In this work, a highly sensitive electrochemical aptasensor for the sensitive detection of streptomycin antibiotic was fabricated based on a novel signal amplification strategy. Specifically, this aptasensor was constructed utilizing porous carbon nanorods (PCNR) formed by porous carbon nanosphere and multifunctional graphene composite (GR–Fe 3 O 4 –AuNPs) as biosensing substrate. PCNR samples with large specific pore volume and high specific surface area were successfully prepared by hydrothermal and chemical activation treatment for the first time. GR–Fe 3 O 4 –AuNPs was served as labels to achieve a high sensitivity and low limit of detection (LOD). Under the optimized conditions, the proposed aptasensor exhibited a high sensitivity and a wider linearity to streptomycin in the range 0.05–200 ng/mL with a low detection limit of 0.028 ng/mL. The proposed aptasensor displayed an excellent analytical performance with great reproducibility, high selectivity and stability. In addition, the as–prepared aptasensor was successfully utilized for the determination of streptomycin in real samples.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Yuanyuan Hui,Ding Yang,Weizhe Wang,Yingying Liu,Chao He,Bini Wang
In this work, a novel electrochemical aptasensor was designed for the sensitive and specific detection of STR in milk samples. First, a gold [email protected] (diallyldimethylammonium chloride) nitrogen-doped carbon nanotube/polyethyleneimine-functionalized metal–organic framework (MOF) ( [email protected] /PEI–MIL-101(Cr)) composite was synthesized and characterized by various technique. The [email protected] /PEI–MIL-101(Cr) composite was then modified on a bare glassy carbon electrode (GCE) surface, providing a favorable platform ( [email protected] /PEI–MIL-101(Cr)/GCE) for aptamer immobilization and current signal amplification. The STR aptamer was grafted to the [email protected] /PEI–MIL-101(Cr)/GCE through the formation of Au–S bonds and π–π stacking interactions. The immobilized STR aptamer binds specifically STR, resulting in an obvious decrease in the current signal. Under the optimal experimental conditions, the linear range of the electrochemical aptasensor for STR detection was 0.01–250 nM which the detection limit (LOD) was calculate as 2.31 nM. This strategy is expected to be a novel platform for the rapid and sensitive detection of STR.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Yuanyuan Hui,Ding Yang,Lusha Wei,Meixue Pu,Yazhou Mao,Xiaoxia Chen,Bini Wang
A novel electrochemical aptasensor for the determination of streptomycin (STR) was constructed in this work. We prepared a palladium nanoparticles/titanium carbide/polyethyleneimine–functionalized nitrogen-doped carbon nanotube (Pd@Ti 3 C 2 -PEI-NCNTs) composite via hydrothermal-assisted formic acid reduction and employed it as a substrate material for anchoring a NH 2 -functionalized aptamer (Apt) to the surface of an electrode through the Pd-N bond. The Pd@Ti 3 C 2 -PEI-NCNTs composite increased the electrochemically active surface area and conductivity of the electrode, achieving substantial signal amplification (1.87 times). The constructed aptasensor demonstrated accurate STR detection via specific recognition between the Apt and STR, with a detection range and limit of 0.01–700 and 0.003 nmol/L, respectively. The aptasensor also exhibited excellent selectivity, stability, reproducibility, practicability and shorter assay times (60 min). Furthermore, molecular docking and molecular dynamic also elucidated the binding mechanism between the aptamer and STR molecules. Overall, this method is expected to be a helpful tool for detecting STR in food.
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Food Analytical Methods
·
2023
·Vol. 16
·期 4
·677-688
·
IF 2.9
Hui Yuanyuan,Peng Haishuai,Li Linqiang,Zhao Aiqing,Yang Ding,Wang Weizhe,Wang Bini
Rapid, accurate, sensitive, and real-time detection of streptomycin (STR) residues in dairy products is very important. In this work, for the first time, the gold nanoflower/polyethyleneimine-functionalized metal organic framework (AuNF-PEI-MIL-101(Cr)) nanocomposites were synthesized via hydrothermal method and characterized using various techniques (SEM, TEM, XRD, FT-IR, and XPS). Then, a novel electrochemical aptasensor was constructed for the detection of STR based on AuNF-PEI-MIL-101(Cr) nanocomposites. The gold electrode (GE) modified with AuNF-PEI-MIL-101(Cr) can immobilize a large number of thiolated STR aptamer via the Au–S bond. When the target STR was in the presence, the current signal was reduced due to the specific binding of STR towards the aptamer. The results indicated that the aptasensor had a wide linear range towards STR (0.01–400 nM) with a low detection limit of 0.003 nM under the optimal conditions. Moreover, the aptasensor exhibited good selectivity, reproducibility, and stability. In addition, the STR in the cow, sheep, and goat milk samples was detected by the spiked recovery method, and satisfactory results were obtained. The method provides an efficient pathway for the sensitive detection of STR.
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