CHINESE JOURNAL OF CHEMICAL PHYSICS
·
2017
·Vol. 30
·期 5
·506-512
·
IF 0.498
Jiang Li-lin,Liu Wei-long,Yang Yan-qiang
The Raman and infrared spectra of all- trans -astaxanthin (AXT) in dimethyl sulfoxide (DMSO) solvent were investigated experimentally and theoretically. Density functional calculations of the Raman spectra predict the splitting of the ν 1 band into ν 1-1 and ν 1-2 components. The absence of splitting in Raman experimental spectra is ascribed to the competition between the two symmetric C=C stretching vibrations of the backbone chain. The ν 1 band is very sensitive to the excitation wavelength: resonance excitation stimulates the higher-frequency ν 1-2 mode, and off-resonance excitation corresponds to the lower-frequency ν 1-1 mode. Analyses of the intramolecular hydrogen bonding between C=O and O−H in the AXT/DMSO system reveal that the C4=O1⋯ H1−O3 and C4′=O2⋯ H2−O4 bonds are strengthened and weakened, respectively, in the electronically excited state compared with those in the ground state. This result reveals significant variations of the AXT molecular structure in different electronic states.
研究领域:
光谱学
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理论化学
·
生物化学
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iScience
·
2021
·
IF 5.458
Zhikai Ye,Wenbo Li,Zhenlong Jiang,Erkang Wang,Jin Wang
Summary Although TGF-β signaling can effectively activate fibroblasts to transform to myofibroblasts, the underlying mechanisms involved in the cell fate switching for trans-differentiation have not been fully elucidated. In this study, we found the evidence of an intermediate state in the process of trans-differentiation. In the early stage of trans-differentiation, cells enter the intermediate state first with multiple characteristics such as accelerating cell cycle, metabolic switching, enhanced anti-apoptotic ability, and pluripotency, which is very similar to the early stage of reprogramming. As the trans-differentiation continues, these characteristics get switched. Therefore, trans-differentiation appears to require the switching of cell proliferation ability, metabolic pathway, and “stemness” to complete the process. In this study, we can conclude that an intermediate state may be necessary with high pluripotency in trans-differentiation from fibroblasts to myofibroblasts. Only after passing the intermediate state, the trans-differentiation is finally completed and will not easily return to the original state.
研究领域:
细胞生物学
·
分子生物学
·
发育生物学
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ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Qingcui Wang,Biru Yang,Yaqi Zhu,Jiameng Pei,Lina Tang,Xiaohua Chen,Guo-Jun Zhang,Yu-Tao Li
Development of sensitive and cost-effective strategies for detecting influenza viruses is crucial to combat the spread of infectious diseases. In this study, a novel trans -dimensional nanocoral gold foam (NCGF) was fabricated on screen-printed carbon electrodes using hydrogen template electrodeposition method. This unique structure, with interconnected large and small pores, significantly increased the specific surface area and stability of the sensor. Based on this nanostructure, the antibodies were further modified and used for influenza A viruses (IAVs) detection by enzyme-linked immunoelectrochemical method. The combination of the large specific surface area of NCGF and the catalytic reaction of alkaline phosphatase led to a dual amplification of electrochemical signals, enabling ultra-sensitive detection of IAVs. The NCGF-based biosensor demonstrated exceptional stability, sensitivity, and achieved a reliable limit of detection as low as 13.14 fg/mL, which is several orders of magnitude improvement in sensitivity over conventional electrochemical immunosensor. Furthermore, 72 clinical samples were analyzed using a portable detection device that could directly read signals from a mobile phone, and the results correlated well with gold standard PCR method. Overall, this rapid, sensitive, and portable assay provides a valuable option for early viral infection diagnosis.
研究领域:
生物传感器
·
病毒学
·
电化学
·
医学诊断
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JOURNAL OF CONTROLLED RELEASE
·
2026
·
IF 12.4
Hengqing Cui,Haijun Hu,Xidong He,Haiyan Cui,Qianqian Ni
Skin regeneration is fundamentally governed by the fibroblast-extracellular matrix (ECM) axis, the disruption of which often drives collagen loss and structural deterioration in skin disorders. However, current interventions typically stimulate fibroblasts indirectly and often yields modest or short-lived outcomes. mRNA therapeutics hold the potential to replenish structural proteins but are limited by poor dermal delivery and transient expression. Here we report the development of dermal fibroblast targeted lipid nanoparticle (LNP) for delivery of trans-amplifying RNA (taRNA) encoding collagen to promote ECM regeneration. This strategy markedly enhanced fibroblast-selective RNA delivery and produced sufficient and durable collagen for up to 7 days following a single intradermal dose. In UVB-induced photoaging models, this collagen taRNA delivered by fibroblast targeted LNPs restored type I collagen deposition, normalized collagen I/III ratio, improved ECM organization, and reduced wrinkle formation. In skin wound models, taRNA nanotherapeutics accelerated wound closure, promoted fibroblast migration, and increased de novo collagen deposition. Histological and biochemical analyses confirmed robust tissue remodeling with minimal local or systemic toxicity. This approach holds promise in aesthetic dermatology and skin disorders.
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ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
·
2023
·Vol. 67
·期 3
·
IF 4.9
Oluwatayo Israel Olasunkanmi,Yanru Fei,Juval Avala Ntsigouaye,Ming Yi,Yao Wang,Jinchang Liu,Weixu Cheng,James Megeto,Tahira Bashir,Yang Chen,Weizhen Xu,Lexun Lin,Wenran Zhao,Yan Wang,Zhaohua Zhong
Enterovirus infections are life-threatening viral infections which occur mainly among children and are possible causes of viral outbreak. Until now, treatment and management of infections caused by members of the genus Enterovirus largely depended on supportive care, and no antiviral medications are currently approved for the treatment of most of these infections. The urgency of discovering new therapeutic options for the treatment of enterovirus infection is increasing. In the present study, we identified that trans-2-hexenoic acid (THA), a natural product from a dietary source, possesses antiviral activity against coxsackievirus B (CVB) and enterovirus A71 (EV-A71) in a dose-dependent manner. We found that THA possesses antiviral activity at 50% effective concentrations (EC50) of 2.9 μM and 3.21 μM against CVB3 and EV-A71 infections, respectively. The time of addition assay revealed that THA inhibits both CVB3 and EV-A71 replication at the entry stage of infection. Additional results from this study further suggest that THA inhibits viral replication by blocking viral entry. Given that THA has received approval as a food additive, treatment of enterovirus infections with THA might be a safe therapeutic option or could pave the way for semisynthetic manufacturing of more antiviral drugs in the future.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2022
·
IF 6.751
Yonghua Zhang,Qiuli OuYang,Bin Duan,Okwong Oketch Reymick,Yue Chen,Yuanzhen Tan,Xiangrong Zhu,Donglin Su,Gaoyang Li,Nengguo Tao
Green mold, caused by Penicillium digitatum , is the primary postharvest disease in citrus fruit. Trans -2-hexenal was found to exert a considerable inhibitory effect on the mycelial growth of P. digitatum with a minimum fungicidal concentration (MFC) of 0.106 g L −1 . To overcome its hydrophobic restriction, a trans -2-hexenal- β -cyclodextrin inclusion complex ( β -CDIC) was prepared by the saturated aqueous solution method. In vitro assays revealed that the MFC of β -CDIC for P. digitatum mycelial growth was 1.00 g L −1 , and 10.0 g L −1 β -CDIC apparently reduced the incidence of green mold in citrus fruit inoculated with P. digitatum while maintaining fruit quality. The structure of β -CDIC was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), fourier transform-infrared spectroscopy (FT-IR), thermogravimetric (TG), and nuclear magnetic resonance (NMR) analyzes. The results showed that the H-1 proton of trans -2-hexenal correlated with the H-5′ proton of β -cyclodextrin ( β -CD), which was further confirmed by molecular docking analysis. The present study suggests that β -CDIC is a promising candidate for controlling postharvest diseases in citrus fruit.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2023
·
IF 4.2
Di Zhang,Shibin Wang,Jiexun Chen,Wanjin Yu,Jianjun Zhang,Wucan Liu,Jianguo Wang
The effective trans-1,3,3,3-tetrafluoropropene (HFO–1234ze(E)) isomerization to cis-1,3,3,3-tetrafluoropropene (HFO–1234ze(Z)) is of great significance due to the wide applications of the product, for example, as heat exchanger, solvent, propellant etc. However, the reaction is limited by the low target product selectivity. In this work, group II metal fluorides (MF2, M = Mg, Ca, Sr, Ba) were prepared as promising catalysts for HFO–1234ze(E) isomerization. Considerable reactivity and selectivity for HFO–1234ze(E) conversion to HFO–1234ze(Z) was obtained, with the conversion rate and selectivity measured to be 16.9–18.7 and 88.7–97.9%, respectively. Analysis of byproduct components further revealed that CF3C≡CH was the dominant main ingredient. Linear relationships between the exposed Brönsted basic site density (F–) and CF3C≡CH byproduct selectivity were obtained, indicating that the increase of Brönsted basic sites could effectively enhance the dehydrofluorination of HFO–1234ze(E), thus promoting the yield of CF3C≡CH. DFT calculations in combination with experimental characterizations were used to systematically investigate the reaction mechanisms for HFO–1234ze(E) isomerization. Multiple reaction pathways were proposed for the HFO–1234ze(Z) target product and CF3C≡CH byproduct formation, with the one-step isomerization and 1C–F defluorination mechanism involved in the reaction pathways. The stronger CF3CH = CH* intermediate chemisorption on the BaF2 surface prohibited the reaction pathway for HFO–1234ze(Z) formation and desorption, in which the CF3CH = CH* intermediate encountered a subsequent dehydrogenation process and resulted in the formation of the CF3C≡CH byproduct. This work provided promising catalysts for HFO–1234ze(E) isomerization with considerable reactivity, selectivity, and stability. More importantly, new insights of Lewis/Brönsted acid-base pair-related catalytic mechanisms for HFO–1234ze(E) isomerization were proposed.
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Catalysis Science & Technology
·
2019
·Vol. 9
·期 13
·3380-3387
·
IF 5.726
Xiaochao Zhang,Jiabing Wei,Guangmin Ren,Changming Zhang,Zhanfeng Zheng,Caimei Fan
This work reported for the first time a systematic investigation on a BiOBr photocatalyzed oleic acid isomerization process in different (air, N2 and O2) atmospheres and proposed the cis–trans isomerization mechanism to effectively restrain the formation of trans fatty acids with deleterious effects on human health. The phase structures, optical properties, and morphologies of as-prepared BiOBr were characterized by XRD, UV-vis DRS, SEM and TEM. Besides, the photocatalyzed isomerization products over BiOBr in different atmospheres were analyzed with FT-IR and 1H NMR for comparison. The results demonstrate that there are significant differences in the photocatalytic isomerization process of oleic acid in different atmospheres, and especially the N2 atmosphere is the most favorable atmosphere, while the presence of O2 will inhibit the formation of elaidic acid. Moreover, density functional theory (DFT) was employed to calculate the charge density and energy difference of ground states between cis- and trans-isomers of oleic acid. Combining the theoretical and experimental findings, the radical cation and photo-induced h+ action mechanisms in the photocatalytic isomerization process were discussed and proposed. Our findings should provide new insights and basic data for understanding the photocatalytic isomerization of unsaturated fatty acids.
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PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2018
·Vol. 20
·期 42
·27205-27213
·
IF 3.906
Lu Zhou,Ligang Chen,Guanhua Ren,Zhongjie Zhu,Hongwei Zhao,Huabin Wang,Weili Zhang,Jiaguang Han
We present terahertz time-domain spectroscopy (THz-TDS) to explore the conformational dynamics of thermally induced and photoinduced isomerization of azobenzene. The essence of the method is that isomerization of azobenzene proceeds via large structural changes in the molecule, while the THz response is sensitive to these changes. We experimentally demonstrate that the THz spectra of azobenzene show remarkable variations upon heating and irradiation, and as such quantitatively recorded and identified THz spectroscopy can be used to monitor the isomerization process. Specifically, the measured THz spectra clearly reveal that the rate of thermal-isomerization from cis-to-trans in non-polar solvents is faster than that in polar solvents, and an about 6-fold acceleration of the rate could be achieved when Au NPs were introduced as a catalyst into azobenzenes. Moreover, we provide evidence that the temperature and Au NP catalyst do not have an obvious influence on the photoinduced isomerization of azobenzene. The presented example illustrates the power of the THz-TDS method to open up a novel avenue for exploring molecular dynamics.
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BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Xueying Lei,Lihua Ding,Xiaonan Yang,Feng Xu,Yongjun Wu,Songcheng Yu
The CRISPR/Cas12a system is increasingly used in biosensor development. However, high background signal and low sensitivity for the non-nucleic acid targets detection is challenging. Here, a p adlock activator which could i nhibit the t rans -cleavage activity of CRISPR/Cas12a system in the intact form by steric hindrance effect (PAIT effect) was designed for non-nucleic acid targets detection. The PAIT effect disappeared when padlock activator was separated into two split activators. To verify the feasibility of padlock activator, a Ca 2+ sensor was developed based on PAIT effect with the assistance of DNAzyme, activity of which was Ca 2+ dependent. In the presence of Ca 2+ , DNAzyme was activated to cleave its substrate, a padlock activator modified with adenine ribonucleotide, into split padlock activators which would trigger the trans -cleavage activity of Cas12a to generate fluorescence. There was a mathematical relationship between the fluorescence intensity and the logarithm of Ca 2+ concentration ranging from 10 pM to 1 nM, with a limit of detection of 3.98 pM. The little interference of Mg 2+ , Mn 2+ , Cd 2+ , Cu 2+ , Na + , Al 3+ , K + , Fe 2+ , and Fe 3+ indicated high selectivity. Recovery ranged from 93.32% to 103.28% with RSD s from 1.87% to 12.74% showed a good accuracy and reliability. Furthermore, the proposed sensor could be applied to detect Ca 2+ in mineral water, milk powder and urine. The results were consistent with that of flame atomic absorption spectroscopy. Thus, PAIT effect is valuable for expanding the application boundary of CRISPR/Cas12a system.
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MOLECULES
·
2023
·Vol. 28
·期 14
·5309
·
IF 4.6
Shi-Hui Lu,Xiao-Na Liang,Xiao-Jin Nong,Ran Chen,Xiu-Xia Li
Trans-p-hydroxycinnamic acid and its esters in the leaves ofLigustrum robustummight be a new resource to prevent diabetes and its complications. In the present study, a new HPLC-UV method using hydrolyzation with sodium hydroxide for quantitation oftrans-p-hydroxycinnamic acid and totaltrans-p-hydroxycinnamic acid esters in the leaves ofL. robustumwas developed, since it was difficult and troublesome to analyze no less than 34trans-p-hydroxycinnamic acid esters by usual HPLC. The extract ofL. robustumwas hydrolyzed with sodium hydroxide at 80 °C for 2 h, and then, hydrochloride was added. HPLC analysis was performed in reverse phase mode using a C-18 column, eluting with methanol-0.1% acetic acid aqueous solution (40:60,v/v) in isocratic mode at a flow rate of 1.0 mL·min−1and detecting at 310 nm. The linear range fortrans-p-hydroxycinnamic acid was 11.0–352.0 μg·mL−1(r2= 1.000). The limit of detection and limit of quantification were 2.00 and 6.07 μg·mL−1, respectively. The relative standard deviations of intra-day and inter-day variabilities fortrans-p-hydroxycinnamic acid were less than 2%. The percentage recovery oftrans-p-hydroxycinnamic acid was 103.3% ± 1.1%. It is the first HPLC method using hydrolyzation for quantification of many carboxylic esters. Finally, the method was used successfully to determinetrans-p-hydroxycinnamic acid and totaltrans-p-hydroxycinnamic acid esters in various extracts of the leaves ofL. robustum. The 60–70% ethanol extracts ofL. robustumshowed the highest contents of freetrans-p-hydroxycinnamic acid (3.96–3.99 mg·g−1), and the 50–80% ethanol extracts ofL. robustumdisplayed the highest contents of totaltrans-p-hydroxycinnamic acid esters (202.6–210.6 mg·g−1). The method can be applied also to the quality control of the products ofL. robustum.Keywords:trans-p-hydroxycinnamic acid;esters;Ligustrum robustum;HPLC-UV;hydrolyzation;sodium hydroxide;quantification
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Humanities & Social Sciences Communications
·
2026
·
IF 4.8
Wen Bing,Lin Zhong
Drawing on translanguaging as a theoretical lens, this article reconceptualizes mistranslation, pseudotranslation, and wandering translation as distinct yet interrelated modes of trans-translating. Examining examples from colonial encounters, feminist writing, and digital culture, it shows how these practices enact epistemic resistance and cultural innovation, subverting linguistic hierarchies and amplifying marginalized voices. Trans-translating transcends source-target binaries to foster transcultural dialogue—yet this dialogue is not politically neutral. Its significance lies in what we theorize as situated accountability: a heuristic that assesses any translational act by asking who it serves, with what intention, under what conditions for reinterpretation, and through what counter-hegemonic spaces. By grounding ethical judgment in these concrete factors, the framework advances equitable exchange not by celebrating fluidity wholesale but by distinguishing emancipatory enactments from harmful ones.
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危险品化学品经营许可证（带存储）