INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Jing Lv,Guangyu Liu,Huimin Gao,Yutao He,Xiaorong Tang,Ziqi Wang,Kaikai Sun,Kulbaram Bayazitova,Zhongliang Jiang
Trehalose, as a food and feed additive, can regulate physiological and pathological processes by activating autophagy, yet the molecular mechanism of trehalose dominated a rise in autophagy has not been elucidated. This study investigated the mechanisms of trehalose-induced autophagy in porcine granulosa cells (PGCs). Trehalose was internalized into PGCs by endocytosis and caused a temporary change of the lysosome pH, ultimately inducing autophagy. Furthermore, miRNA-seq analysis of PGCs indicated that trehalose upregulated the expression of miR-221-5p_R-4, which can target nuclear receptor binding factor 2 (NRBF2) to induce autophagy of PGCs. In summary, this study reveals that miR-221-5p_R-4 targeting NRBF2 was involved in the autophagy of PGCs challenged by trehalose, which was taken up by PGCs through endocytosis.
研究领域:
分子生物学
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细胞生物学
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生殖生物学
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产品关联
DOI
PubMed
高影响因子
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
·
2026
·Vol. 116
·期 3
·117523
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IF 1.8
Huifeng Yang,Xiaoyi Li,Yimin Su,Jianxin Guo,Tengfei Wang,Zhigang Wang,Hai Fu,Zhi Chen,Zhongjian Xie,Defa Li
Purpose Acute respiratory infections caused by bacterial and viral pathogens pose a major global health burden. The recombinase polymerase amplification (RPA)-CRISPR-Cas12a system offers a promising point-of-care testing (POCT) platform, but its field deployment is limited by the instability of lyophilized reagents. This study aims to develop a composite lyoprotectant to enhance the stability of the RPA-CRISPR-Cas12a system for respiratory pathogen detection. Materials and methods A composite lyoprotectant composed of trehalose and carboxymethyl chitosan (Tre-CMC) was formulated at various mass ratios. The optimal ratio was identified by evaluating matrix microstructure, enzyme activity retention, and primer-dimer suppression. The lyophilized system was tested for sensitivity, specificity, and long-term stability against six respiratory pathogens (H1N1, IBV, Neisseria meningitidis , SARS-CoV-2, Streptococcus pneumoniae , and human adenovirus) using real-time fluorescence and gel electrophoresis. Results This study establishes a promising proof-of-concept framework for an integrated, lyophilized RPA-CRISPR–Cas12a diagnostic system. The optimal Tre: CMC ratio (2:1) produced a porous, non‑hygroscopic matrix that preserved reagent integrity. The lyophilized system achieved a detection limit of 10 copies/reaction within 30 min for all six pathogens, with 100% specificity. After six months of storage at 4°C, RPA enzyme activity remained above 89%, and Cas12a-crRNA complex functionality was fully retained. Tre-CMC significantly reduced primer-dimer formation and nonspecific background fluorescence. While clinical evaluation using 21 nasopharyngeal swab samples demonstrated 100% concordance with RT-qPCR—preliminarily supporting the system's potential diagnostic accuracy—we acknowledge that this initial methodological framework requires broader and more rigorous downstream validation with larger clinical cohorts to formally establish its robust diagnostic performance. Conclusion The Tre-CMC composite lyoprotectant effectively stabilizes the RPA-CRISPR-Cas12a system, enabling cold-chain-independent storage and reliable POCT for respiratory pathogens in resource-limited settings.
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DOI
PubMed
高影响因子
Frontiers in Plant Science
·
2022
·
IF 6.627
Li Bingyan,Guo Tengfei,Zhou Wei
Despite the fact that there are many distinct types of plant growth regulators (PGRs), the diverse ways in which they regulate plant development are rarely compared. In this study, four PGRs (trehalose, chitosan, humic acid and gamma-aminobutyric acid) were selected and sprayed folially, and plant samples were collected while maize was at vegetative leaf stages 6, 10, and 14 (V6, V10, and V14, respectively) to reveal the effects of different PGRs on photosynthesis, dry matter accumulation, oxidative stress, carbon and nitrogen metabolism, hormone levels, and gene expression of maize. Results showed that 100 mg/L PGRs did not induce oxidative damage or repair activities in maize. Trehalose significantly increased chlorophyll content at V6 and promoted dry matter (roots and shoots) accumulation at V6 and V10. The activities of carbon and nitrogen metabolizing enzymes were significantly enhanced by trehalose treatment, which promoted the accumulation of sucrose and soluble sugar, but did not affect the biosynthesis of auxin and gibberellin at V6. Changes in carbon and nitrogen metabolism enzymes are regulated by transcription of related synthetic genes. Lower starch content and higher sucrose content in trehalose-treated maize leaves are important biological characteristics. Further analysis revealed that the effect of trehalose on the metabolic activity of maize was a short-term promoting effect (0–12 days), while the effect on maize growth was a long-term cumulative effect (13–28 days). Overall, exogenous trehalose induced stronger carbon and nitrogen metabolic activity, higher photosynthetic capacity and more dry matter accumulation than chitosan, humic acid and gamma-aminobutyric acid.
研究领域:
农业科学
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植物生理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2022
·
IF 9.336
Lixin Kang,Qiufang Liang,Arif Rashid,Abdul Qayum,Zhuzhong Chi,Xiaofeng Ren,Haile Ma
A novel food packaging film was developed by incorporating a tea polyphenols-loaded pullulan/trehalose (TP@Pul/Tre) into a composite film with ultrasound-assisted treatment of dual-frequency (20/35 kHz, 40 W/L) for 15 min to assess the physicochemical and mechanical properties of a composite film. The optimized ultrasound-assisted significantly increases elongation at break, tensile strength, and improves the composite film's UV/water/oxygen barrier properties. Structure analysis using attenuated total reflectance-Fourier transform infrared, X-ray diffraction and thermal stability revealed that these improvements were achieved through ultrasound-enhanced H-bonds, more ordered molecular arrangements, and good intermolecular compatibility. Besides, the ultrasound-assisted TP@Pul/Tre film has proven to have good antibacterial performance against Escherichia coli and Staphylococcus aureus, with approximately 100 % lethality at 4 h and 8 h, respectively. Moreover, the ultrasound-assisted TP@Pul/Tre film effectively delayed moisture loss, oxidative browning, decay, and deterioration in fresh-cut apples and pears, thereby extending their shelf life. Thus, ultrasound has proved to be an effective tool for improving the quality of food packaging films, with a wide range of applications.
研究领域:
食品科学
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材料科学
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生物化学
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DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2022
·
IF 5.546
Zhi Li,Lifen Zhang,Changning Xue,Yuman Zhang,Yao Yu,Xinhong Guo,Zhenzhong Zhang
Hydroxypropyl-β-Cyclodextrin (HP-β-CD) was used to form the HP-β-CD/Oridonin inclusion complex. Then, the novel Multi-loaded nanovesicles systems (NVs) were established to encapsulate both hydrophilic (Trehalose) and hydrophobic drugs (Oridonin inclusion complex) with peptides. NVs had an average size of 224.4 ± 10.84 nm and a zeta potential of 17.83 ± 1.05 mV. Furthermore, these NVs significantly decreased lipid over-accumulation, and reverse the formation of foam cells in RAW264.7 cells. Oridonin and Trehalose in NVs provided significant synergistic effects to inhibit lipid over-accumulation (CI < 1). In addition, NVs could modulate the release of IL-1β, IL-6 and TNF-α, promote the formation of autophagosomes. Taken together, the Multi-loaded NVs may be used as an effective drug delivery system, providing new insights into decrease the formation of foam cells and regulate the inflammation.
研究领域:
药学
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生物技术
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细胞生物学
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药物递送系统
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产品关联
DOI
高影响因子
Bioresources and Bioprocessing
·
2026
·Vol. 13
·期 1
·57
·
IF 6.3
Liu Bohua,Guo Qingqing,Wang Shuo,Shi Ting,Lu Fuping,Zhang Yi-Heng P. Job
Trehalose 6-phosphate (T6P), often referred to as plant insulin, serves as a central signaling molecule that regulates carbon partitioning and sucrose flux in plants, thereby influencing key agronomic traits, such as grain yield and drought resilience. Foliar spraying of T6P has been shown to significantly enhance yield and stress tolerance of numerous grains and vegetables. To circumvent the dependency on coenzymes in natural T6P synthesis, we designed and validated an in vitro new-to-nature, coenzyme-free, minimal enzymatic pathway for the biosynthesis of T6P from maltose and polyphosphate. This three-enzyme cocktail contained maltose phosphorylase, trehalose 6-phosphate phosphorylase, and polyphosphate glucokinase, and did not involve any costly coenzymes, such as ATP or UDP. Through systematic optimization of experimental parameters (including pH, temperature, Mg²⁺, phosphate concentration, and enzyme ratios), a 93% molar yield of T6P was achieved from 10 g/L maltose. The scale-up of this in vitro bioprocess to a 100-mL bioreactor with 200 g/L maltose enabled the production of up to 541 mM T6P (i.e., 252 g/L T6P disodium salt) within two hours, corresponding to a very high volumetric productivity of 126 g/L/h. This study established a scalable and cost-competitive in vitro biomanufacturing of T6P. Chemical intervention based on timed foliar spraying of T6P to cultivated crops offers a simpler and safer agricultural practice compared to genetic modification of crops. Graphical abstract The alternative text for this image may have been generated using AI.
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2024
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IF 8.5
Lian Jiang,Yongli Tian,Haide Zhang,Shisheng Liu
The high temperature induces conformational changes in β-glucosidase, making it inactive and limiting its application field. In this paper, the effect of trehalose on the thermostability of β-glucosidase from low-moisture Hevea brasiliensis seeds was investigated. The results showed that the residual enzyme activities of β-glucosidase supplemented with trehalose after high-temperature treatment were significantly higher than that of the control group. The improvement of thermostability could be explained by low-field nuclear magnetic resonance (LF-NMR) and molecular dynamics (MD) simulations at the molecular level. Moreover, adding trehalose increased the water activity and water content of β-glucosidase, leading to a more stable conformation. Trehalose replaced some water and formed a stable network of hydrogen bonds with protein and surrounding water. The glass formed by trehalose also reduced molecular movement, thus providing good protection for enzymes.
研究领域:
酶学
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生物化学
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生物物理学
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产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2025
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IF 16
Lingmei Wang,Huicai Wang,Junlun Cao,Jicheng Yan,Changwei Dai,Wuzhu Sun,Qingyang Du,Zhiqiang Huang,Dan Liu,Chao Li,Jingyu Sun
Aqueous Zn-ion batteries utilizing moldable gel electrolytes are expected to meet power requirements for wearable devices because of their inherent safety and energy output. Nevertheless, comprehensive modulation over the mechanical robustness, water retention capability, and electrode–electrolyte interface stability remains at a nascent stage. Drawing inspiration from the naturally cryoprotective and hygroscopic properties of trehalose, we herein devise a strategy by incorporating trehalose into polyacrylamide hydrogel electrolytes, targeting the construction of wearable Zn-ion batteries. The optimized hydrogel electrolyte demonstrates low-temperature adaptability (−15 °C), high-temperature stability (50 °C), and water retention capability while helping to suppress dendrite growth and parasitic reactions. Theoretical calculations and electrochemical characterizations reveal that trehalose modifies the Zn-ion solvation structure and optimizes the electrode–electrolyte interface. The thus-fabricated Zn-ion batteries exhibit favorable electrochemical performances in a wide-temperature range, achieving a capacity retention of 87.2% after 2000 cycles at 5 A g–1. The assembled pouch cell could also be sustained for more than 500 cycles. Moreover, the integration of our Zn-ion batteries with Si solar cells to construct a wearable solar-charging system enables an energy conversion efficiency exceeding 10%.
研究领域:
材料科学
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电化学
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储能技术
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高分子科学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2021
·
IF 3.963
Chao Xu,Hongzhou Chen,Qinyan Wu,Yuqi Wu,Paul Daly,Jian Chen,Hongfu Yang,Lihui Wei,Yiqing Zhuang
Fusarium head blight( FHB )caused by Fusarium graminearum species complex (FGSC) is one of the most important diseases around the world. Deoxynivalenol (DON) is a type of mycotoxin produced by FGSC when infecting cereal crops . It is a serious threat to the health of both humans and livestock. Trehalose-6-phosphate phosphatase ( TPP ), a conserved metabolic enzyme found in many plants and pathogens, catalyzes the formation of trehalose. N-(phenylthio) phthalimide (NPP) has been reported to inhibit the normal growth of nematodes by inhibiting the activity of TPP , but this inhibitor of nematodes has not previously been tested against F. graminearum . In this study, we found that TPP in F. graminearum (Fg TPP ) had similar secondary structures and conserved cysteine (Cys356) to nematodes by means of bioinformatics. At the same time, the sensitivity of F. graminearum strains to NPP was determined. NPP exhibited a better inhibitory effect on conidia germination than mycelial growth . In addition, the effects of NPP on DON biosynthesis and trehalose biosynthesis pathway in PH-1 were also determined. We found that NPP decreased DON production, trehalose content, glucose content and TPP enzyme activity but increased trehalose-6-phosphate content and trehalose-6-phosphate synthase ( TPS ) enzyme activity. Moreover, the expression of TR I 1 , TR I 4 , TR I 5 , TR I 6 , and TPP genes were downregulated, on the contrary, the TPS gene was upregulated. Finally, in order to further determine the control ability of NPP on DON production in the field, we conducted a series of field experiments, and found that NPP could effectively reduce the DON content in wheat grain and had a general control effect on FHB. In conclusion, the research in this study will provide important theoretical basis for controlling FHB caused by F. graminearum and reducing DON production in the field.
研究领域:
植物病理学
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真菌学
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农学
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毒理学
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DOI
PubMed
高影响因子
FASEB JOURNAL
·
2020
·Vol. 34
·期 6
·8402-8415
·
IF 4.966
Xingquan Xu,Rongliang Wang,Rui Wu,Wenjin Yan,Tianshu Shi,Qing Jiang,Dongquan Shi
Bone loss is a severe complication of primary biliary cirrhosis (PBC). Trehalose was intermittently administered in bile duct-ligated (BDL) male rats, a PBC-related osteoporosis model, for 4 weeks to reduce osteoporosis. Femoral bones were assessed ex vivo by micro computed tomography (CT) and histomorphometry. The potential mechanisms related to the reduction of osteoporosis were explored by evaluating the effect of trehalose on osteoblast autophagy, osteogenesis, osteoclastogenesis, and ERK phosphorylation. The results demonstrated that trehalose reduced osteoporosis of BDL rats and decreased osteoblast-mediated osteoclast differentiation by enhancing osteoblast autophagy to regulate osteoprotegerin (OPG) secretion. Hydroxychloroquine (HCQ) increased the expression of OPG and OPG /receptor activator genes for nuclear factor-κB ligand (RANKL) ratio, and reduced osteoblast-mediated osteoclastogenesis by inhibiting autophagy flux and inducing autophagosome formation. Furthermore, trehalose increased the phosphorylation of ERK1/2 in MC3T3-E1 cells, and the ERK inhibitor PD98059 reversed the upregulation of OPG gene and reduction of trehalose-induced osteoclastogeneis. The treatment with HCQ markedly increased the ERK phosphorylation. The correlation between autophagosome formation and ERK phosphorylation was confirmed in autophagy proteins (ATG) 4B or ATG5-deficient cells. Thus, trehalose could decrease osteoblast-mediated osteoclastogenesis and reduce PBC-related bone loss by regulating ERK phosphorylation via autophagosome formation.
研究领域:
药理学
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病理学
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分子生物学
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骨科
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 103
·期 3
·1194-1204
·
IF 4.125
Pengyu Zhu,Chunmin Ma,Jing Fan,Yang Yang,Xiaofei Liu,Xin Bian,Likun Ren,Yue Xu,Dehui Yu,Linlin Liu,Yu Fu,Jian Gao,Na Zhang
BACKGROUND Soybean 11S globulin has good functional properties, which are widely used in the field of food. However, natural soybean 11S globulin (N-11S) has low flexibility and is easy to aggregate, impacting its foaming process. Studies have shown that soybean 11S globulin in molten globule state (MG-11S) has better molecular flexibility than N-11S, and trehalose has been shown to improve the properties of proteins. Therefore, this study investigated the interaction mechanism between trehalose and MG-11S, and its impact on rheological and foaming properties of MG-11S. RESULTS The molecular docking and intrinsic fluorescence results showed that hydrogen bonding was the main interaction force at lower than 0.5 mol L −1 trehalose added. Meanwhile, rheology and foaming showed that the MG-11S–trehalose complexes had better viscoelasticity, foaming ability (66.67–86.67%) and foaming stability (75.00–89.29%) than N-11S (16.67% foaming ability and 40.00% foaming stability); however, when the trehalose was higher than 0.5 mol L −1 , molecular crowding occurred and H-bonds were weakened, resulting in reduction of foaming capacities. Microstructure determination showed that trehalose attached to the surface of foam membrane; meanwhile, the foaming structure of the complex with 0.5 mol L −1 trehalose had a thicker liquid film with decreased drainage rate, less agglomeration and disproportionation of foam, illustrating the best foaming ability and foaming stability. CONCLUSION The results suggested that trehalose at different concentrations can interact with MG-11S through different mechanisms, and improve the foaming capacity of MS-11S. This provided a reference for the application of MS-11S in foaming food. © 2022 Society of Chemical Industry.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
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2020
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IF 3.701
Zhenbang Xiao,Shengtao Yang,Jiali Chen,Chengyong Li,Chunxia Zhou,Pengzhi Hong,Shengli Sun,Zhong-Ji Qian
Ultraviolet radiation B cause photoaging of the skin by enhancing the high expression of matrix metalloproteinases (MMPs) to degrade collagen and inhibit the synthesis of procollagen. Trehalose (TRE), a widely occurring natural disaccharide, has been shown to play a cytoprotective role in many pathological processes. However, few studies have evaluated whether it has anti-photoaging potential. In this study, we investigated the anti-photoaging and mechanism of action of TRE using Human immortalized keratinocytes (HaCaT) cells. The results indicated that TRE pretreatment effectively scavenges reactive oxygen species (ROS) accumulation induced by UVB and increases the content of certain endogenous antioxidant factors in HaCaT cells. TRE suppressed UVB-induced MMP expression by blocking mitogen-activated protein kinases (MAPKs), activator protein 1 (AP-1) and NF-κB signaling. TRE treatment increased procollagen I synthesis via activating transforming growth factor-β (TGF-β)/Smad pathway and demonstrated that TRE may be a potential natural disaccharide for photoaging therapy in the future.
研究领域:
皮肤科
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细胞生物学
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药理学
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DOI
高影响因子
危险品化学品经营许可证（带存储）