RSC Medicinal Chemistry
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2024
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IF 4.1
Ji-Chao Guo,Shi-Hui Deng,Shu-Min Zhou,Xuan Zhou,Jiajia Du,Si-Han Zhou,Qi-Hua Zhao,Zhong-Yan Cai,Xiaoxia Ren,Ming-jin Xie
There is significant value in developing multifunctional drug delivery systems with high therapeutic efficiency for diagnosing and treating tumors. In this study, we synthesized the ATP-triggered and pH-sensitive material ZIF-90 using the liquid-phase diffusion method. This was done to load 10-hydroxycamptothecin (HCPT), and the FA-PEG-NH2 conjugate was synthesized through an amidation reaction. We further modified the HCPT@ZIF-90 nanocomposite by employing the Schiff base reaction to create the HCPT@ZIF-90-PEG-FA nanomaterial. Drug loading test results revealed a high HCPT drug loading of up to 22.3% by weight. In the drug release experiment, the cumulative drug release of HCPT@ZIF-90 nanomaterials in pH 5.4 and ATP solutions was the highest after 72 hours. The active targeted delivery of FA and the dual-responsive release of HCPT by ZIF-90 significantly enhanced the therapeutic effect of HCPT@ZIF-90-PEG-FA on human colon cancer cells (HCT116). In the cytotoxicity test, when 100 μg mL−1 of HCPT@ZIF-90-PEG-FA was incubated with cells, the cell survival rate was 16.61 ± 1.19%, significantly lower than that of the other experimental groups. This result indicates that HCPT@ZIF-90-PEG-FA exhibits excellent anti-tumor activity. Cell cycle experiments have shown that HCPT@ZIF-90-PEG-FA may inhibit the proliferation of cancer cells by blocking DNA synthesis and halting cell cycle progression. Cell uptake experiments showed that HCPT@ZIF-90-PEG-FA was mainly present in the cytoplasm of HCT1116 cells, indicating successful cellular entry of the drug to exert its therapeutic effect. In vivo experiments also demonstrated that HCPT@ZIF-90-PEG-FA nanomaterials can effectively eradicate HCT116 tumors. The utilization of the nano-drug carrier ZIF-90, along with the modification with PEG-FA, notably improved the therapeutic efficacy of HCPT. These results suggest that the system, with its active targeted delivery of FA and dual-responsive release of HCPT, could present a novel strategy for treating human colorectal cancer.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Donghui Zhao,Yunhao Deng,Junyi Shi,Xinye Ni,Chaoqing Li,Yang Bai,Yang Xuan,Jianhao Wang
Nanozymes with multienzyme activity for reactive oxygen species (ROS) generation and intracellular redox imbalance are attractive strategy for cancer therapy. However, it is severely limited by low biocompatibility and catalytic efficiency, hypoxic and high levels of GSH in the tumor microenvironment. To address these issues, a copper doping carbon nanozyme (CC) with multienzyme activity was designed and integrated with photosensitizer Ce6 and gelatin to fabricate ROS amplifier (CCC). Gelatin endowed CCC with good biocompatibility, low hemolysis, and enzyme responsive degradation. CCC with high CAT-like, POD-like, OXD-like, and GSHox-like activities can induce the intracellular ROS storm formation to eliminate the cancer cells. The OXD-like activity and PDT performance mediated 1 O 2 generation was markedly potentiated by the CAT-like activity of CCC via catalyzing high expression of H 2 O 2 to generate O 2 . At the same time, a large amount of ·OH were produced through POD-like activity of CCC and GSH was depleted by the GSHox-like activities of CCC, resulting in a destructive ROS storm formation and cellular redox homeostasis disruption. Both in vivo and in vitro experiments showed that CCC displayed satisfactory anti-tumor activity and biocompatibility. Our work provides a novel strategy for the development of nanozyne enhanced photodynamic therapy of cancer.
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CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Zhijun He,Xiaoqian Li,ZhaoXing Yang,Xiaoqing Liu,Fang Xie,Xuqin Lv,JiaZuan Ni,Shuiyuan Cheng,Qiong Liu,Jie Cai
Neuroinflammation, caused by abnormal activation of glial cells, plays an important role in nerve injury, which can lead to cognitive impairment. Consequently, regulating glial cell activation and subsequent generation of inflammatory mediators is valuable for the treatment of neuroinflammation-based brain diseases. Selenium (Se) and folic acid (FA) are two key nutritional supplements that have bioactivities associated with regulating neuroinflammatory responses, resisting oxidative stress, and preventing neuronal apoptosis in vivo. Based on this, we prepared multifunctional nanoparticles using octenyl succinic anhydride (OSA)-modified starch (OSAS) to carry both Se and FA, with the aim to facilitate synergistic resistance of neuroinflammatory diseases. The FA-grafted OSAS molecules self-assembled into nanomicelles and functioned as nanocarriers to load selenium nanoparticles (SeNPs) (a unique form of Se supplement) and form novel nanoparticles FA-OSAS-SeNPs with a protective shell of starch. Furthermore, the effect and mechanism of FA-OSAS-SeNPs on inflammatory responses were investigated using lipopolysaccharide (LPS)-induced BV2 microglial cells and BALB/c mice. Our results showed that FA-OSAS-SeNPs ameliorated LPS-induced spatial learning and memory impairment. Furthermore, FA-OSAS-SeNPs significantly suppressed the activation of glial cells, the expression of oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and the release of inflammatory factors. Moreover, FA-OSAS-SeNPs prominently alleviated endoplasmic reticulum (ER) stress and LPS-induced overactivation of the NF-κB and MAPK signaling pathways. In conclusion, these results demonstrate that FA-OSAS-SeNPs represent a promising candidate in alternative/supplementary medication or functional foods for treating inflammatory brain diseases.
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Materials Today Bio
·
2025
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IF 10.2
Jiangpeng Pan,Juntao Wang,Wei Wang,Ziyang Liu,Shuai Huo,Lei Yan,Wei Jiang,Fengmin Shao,Yue Gu
Mitochondrial dysfunction-induced oxidative stress is a key pathogenic factor in acute kidney injury (AKI). Despite this, current mitochondrial-targeted antioxidant therapies have shown limited efficacy in clinical settings. In this study, we introduce a novel renal-clearable and mitochondria-targeted antioxidant nanozyme (TPP@RuCDzyme) designed to precisely modulate mitochondrial oxidative stress and mitigate AKI progression. TPP@RuCDzyme was synthesized by integrating ruthenium-doped carbon dots (CDs) with triphenylphosphine (TPP), a mitochondria-targeting moiety. This nanozyme system exhibits cascade enzyme-like activities, mimicking superoxide dismutase (SOD) and catalase (CAT), to efficiently convert cytotoxic superoxide (O 2 • - ) and hydrogen peroxide (H 2 O 2 ) into non-toxic water (H 2 O) and oxygen (O 2 ). This dual-enzyme mimicry effectively alleviates mitochondrial oxidative damage, restores mitochondrial function, and inhibits apoptosis. Compared to RuCDzyme alone, TPP@RuCDzyme demonstrated significantly enhanced efficacy in alleviating glycerol-induced AKI by inhibiting oxidative stress. By leveraging the catalytic activity derived from the integration of CDs and a metallic element, this study presents a promising therapeutic strategy for AKI and other renal diseases associated with mitochondrial dysfunction.
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MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Zhongqiu Zhao,Zixuan Zhou,Lei Wang,Ying Zhang,Yaguang Sun,Mingchang Zhu,Shuangyan Wu
Riboflavin was first isolated from milk and widely used in food, biological and pharmaceutical fields. Accurate detection of riboflavin content is of great significance for the treatment of related diseases and the supervision of food/drug quality. In this paper, a 1D chain copper-based coordination polymer with excellent water stability was synthesized by hydrothermal method using rigid ligand 1,10-phenanthroline and flexible ligand 1,4-phenylenedioxydiacetic acid, named Cu-phen-PEDA. Based on the Förster resonance energy transfer (FRET) and dynamic quenching detection mechanism, the emission spectrum of Cu-phen-PEDA overlaps with the absorption spectrum of riboflavin. As an energy donor in this case, Cu-phen-PEDA significantly increases the fluorescence intensity of riboflavin (energy acceptor), while simultaneously decreasing its own fluorescence intensity, which is an effective proportional-building strategy. In the lower concentration range, riboflavin can be specifically recognized by ratiometric fluorescence with a good linear relationship, and the detection limit is as low as 23 nM. The potential aspects of its specificity, stability, reusability and practical application were explored.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Fansu Meng,Panpan Wang,Lina Yang,Junjie Yan,Anil K. Giri,Jaiwoo Lee,Kosuke Kusamori,Makiya Nishikawa,Shoko Itakura,Honghui Gu,Qi Li,Zhong Chen,Zhenjiang Yang,Detang Li,Yu Cai
Breast cancer (BC) stands as one of the primary malignancies threatening women's health. Baicalin (BA) exhibits anti - BC effects, yet it is plagued by issues such as low water solubility and poor bioavailability. To enhance the anti-BC efficacy of BA, this study developed a pH - responsive albumin nanoparticle loaded with BA (BA-AN-FA@mPEG). The optimized BA-AN-FA@mPEG features an average particle size of (185.8 ± 2.36) nm, a Zeta potential of (−32.70 ± 1.27) mV, an encapsulation efficiency of approximately 73.82 %, and a drug loading of about 5.68 %. The aqueous solution of BA-AN-FA@mPEG demonstrates excellent stability at 4 °C, with no significant drug leakage occurring within 12 weeks. BA-AN-FA@mPEG shows a certain sustained - release effect, and the drug release rate is faster under acidic conditions. The results of efficacy evaluation indicate that the half - maximal inhibitory concentration of BA-AN-FA@mPEG against MDA-MB-231 cells is 26.34 μg/mL. It can promote cellular uptake, effectively induce apoptosis, and cause cell cycle arrest. In summary, BA-AN-FA@mPEG is a promising tumor - targeted therapeutic formulation that can improve drug stability, tumor delivery efficiency, and therapeutic efficacy.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2025
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IF 4.6
Min Sun,Xujie Wang,Lingwei Bu,Yawen Fan,Zihan Guo,Yingying Shan,Xiao Zhu,Lei Hu,Zhengquan Yan
Herein, a novel Bi 3+ -doped Au-Ag quantum dot (Bi 3+ -Au-Ag QD) system was synthesized, by homogeneous reduction method using AgNO 3 , Bi(NO 3 ) 3 ·5 H 2 O and HAuCl 4 ·4 H 2 O as raw materials, for multi-channel detection of S 2− in agricultural foods. Owing to the strong interaction between S 2− and the Ag/Au components, trace amounts of S 2− led to the formation of Ag 2 S or Au 2 S sulphides on the surface of the Bi 3+ -Au-Ag QDs. This surface modification altered the intrinsic fluorescence property of QDs, and their catalytic ability to the oxidization of colourless 3,3′,5,5′-tetramethylbenzidine (TMB) into its blue oxide (oxTMB), resulting in noticeable changes in spectral responses, colour and RGB values. Under optimised experimental conditions, Bi 3+ -Au-Ag QDs were employed successfully for ‘turn-on’ fluorescence, UV–vis and smartphone-assisted red-green-blue (RGB) detection of S 2− in real agricultural food samples. The limits of detection –4.75 × 10 −10 , 6.48 × 10 −9 and 7.22 × 10 −9 mol·L −1 respectively, well met the safety threshold set by the World Health Organization.
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Advanced Healthcare Materials
·
2025
·
IF 9.6
Fengjie Liu,Fansu Meng,Xiaoshan Hong,Anil K Giri,Mulazim Hussain Asim,Zhong Chen,Yiqi Chen,Yixuan Lin,Lulu He,Qiaowen Bu,Yong Huang,Xiaoli Sun,Zhenjiang Yang,Yu Cai
The inflammatory microenvironment (IME) is closely related to the progression of triple-negative breast cancer (TNBC). As an anti-inflammatory active compound, poor solubility and targeting limit the potential of baicalin (BA) to modulate IME in TNBC. In this study, BA loaded bifunctional albumin nanoparticles (BANP) with long circulation/acid-sensitive release and active targeting functions are developed to improve the delivery efficiency of BA. BANP have a spherical core–shell structure, with appropriate particle size (79.63 ± 1.91 nm), zeta potential (−33.7 ± 0.82 mV), and encapsulation efficiency (72.71%), and are released faster in an acidic environment. BANP can enhance the uptake by TNBC cells and “M2” macrophages, prolong circulation time in the blood, and increase accumulation at tumor sites in vivo. It induces cell cycle arrest and early apoptosis in MDA-MB-231 cells and inhibits tumor growth in 4T1 tumor-bearing mice. Additionally, BANP reduce tumor immune infiltration, promote macrophage recruitment and anti-inflammatory M2 polarization, and enhance the phagocytic activity of M2 macrophages. Transcriptome sequencing and cytokine profile analysis reveal that BANP regulate TNBC IME through multitarget mechanisms related to cell cycle, inflammatory response, metabolism, etc. In summary, this study provides a nanodelivery platform with broad application potential and a new strategy for the regulation of IME.
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ACS Nano
·
2025
·
IF 16
Chenyu Zhao,Rong Wang,Siyuan Luo,Ya Wang,Zhenhai Gan,Jingran Di,Daocheng Wu
To enhance synergistic therapeutic efficiency of tumor photothermal–thermodynamic–chemotherapy and address challenges of limited laser penetration, uneven heating, and poor temporal coordination among therapeutic modalities, we fabricated a photomicroneedle (PMN) system consisting of an optical fiber integrated into a perforated stainless-steel microneedle, in which the laser delivered via the PMN is emitted both from the tip and the sidewalls, enabling uniform intratumoral irradiation. The PMN-assisted tumor continuous high-intensity photothermal–thermodynamic alternate cycle therapy and chemotherapy strategy that consistently keeps over 90% tumor cell death within 48 h was developed. To implement this strategy, dual-layer nanocomposites were engineered by self-assembling IR825 and doxorubicin (DOX) into core nanoparticles (Dp825 NPs), which were then encapsulated in polydopamine (PDA)–Fe(III)–folic acid (FA) infinite coordination polymer (ICP) shell loaded with artesunate (ARS), forming nanocomposites (Dp825:ARS@PDA–Fe(III)–FA ICP NCPs). The resulting nanocomposites have high drug loading and tumor targeting efficiency, enabling precise spatiotemporal control of drug release to meet the requirements of the above coordinated strategy. In vivo studies demonstrated complete eradication of large tumors (∼300 mm3) without recurrence over 60 days, using only 300 mW·cm–2 laser power and a total injection dose of 5.0 mg·kg–1, which offers a promising platform for highly effective and minimally side-effect tumor therapy.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-21
·
IF 15.7
Yang Mei,Huang Yida,Chen Zhongxing,Ye Qianfang,Zeng Zhenhai,You Xinru,Bao Bijun,Xing Wenqian,Zhao Nan,Zou Zhilin,Pan Hongxian,Chen Xin,Gao Qingyi,Chen Tingting,Zhang Shenrong,Chen Aodong,Xiao Yichen,Xu Huilin,Li Jiawei,Li Yongjiang,Kong Na,Tao Wei,Zhou Xingtao,Huang Jinhai
Photothermal therapy (PTT), a vanguard strategy in cancer/ocular neovascularization treatment, has attracted considerable attention owing to its precision, controllability, high efficacy, and minimal side effects. Nevertheless, its inherent limitations necessitate innovative solutions. One promising strategy is to develop reagents with enhanced photothermal conversion efficiency under long-wavelength laser irradiation. Carbon nanomaterials, known for their broad absorption spectra, are currently hindered by single-wavelength lasers in clinical treatments. In this study, we address this limitation by coating mesoporous carbon nanomaterials (MCNs) with a lanthanide oxysulfide up-conversion material (Y 2 O 2 S:Yb 3+ ,Er 3+ ), converting 980 nm light into visible light. This advancement enhances the photothermal conversion efficiency of the produced MCNs/Ln/GD/FR nanocomposites from 59.48% to 82.86%. Furthermore, the incorporation of gambogic acid and doxorubicin intensifies the synergistic photothermal therapy effect. A dual stimuli-responsive hydrogel (PNIPAM) is employed to ensure controlled drug release and safe delivery to tumors. Evaluations demonstrate that the MCNs/Ln/GD/FR nanocomposites exhibit exceptional tumor targeting and evident photothermal synergistic therapy effects on both subcutaneous and ocular in situ melanoma tumors by activating tumor-suppressive signaling pathways while inhibiting proliferation and differentiation-related pathways. These findings might pave the way for the development of photothermal reagent and offer valuable insights for advancing therapeutic strategies.
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ACTA PHYSICO-CHIMICA SINICA
·
2025
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IF 13.5
Zihan Cheng,Kai Jiang,Jun Jiang,Henggang Wang,Hengwei Lin
Integrating stimuli-responsive luminescence with dynamic emission properties offers a powerful strategy to enhance information encryption through multi-level authentication systems. By rationally tuning the singlet-triplet energy gap (Δ E ST ) of a material, simultaneous activation of phosphorescence (Phos) and delayed fluorescence (DF) can be achieved, enabling programmable dynamic afterglow behavior. In this work, we report the first carbon dot (CD)-based thermoresponsive dynamic afterglow material, synthesized via in situ covalent immobilization of CDs within a cyanuric acid matrix. The resulting system demonstrates a thermally driven green-to-blue afterglow transition across a wide temperature range (273.15–423.15 K), exhibiting dual-mode thermochromic afterglow (TCA) and time-resolved afterglow (TRA) characteristics. Notably, a blue-to-green afterglow transition occurs above the threshold temperature of 348.15 K, where TRA dominates due to temperature-dependent exciton redistribution. This synergistic TCA-TRA interplay endows the material with unprecedented dynamic afterglow modulation capabilities. Structural and photophysical analyses confirm that covalent fixation reduces the Δ E ST of CDs from 0.46 to 0.28 eV, as designed. This ΔE ST engineering enables thermal control over the Phos/DF equilibrium, directly governing the observed dynamic emission. Finally, the potential applications of the prepared material in thermal monitoring and high-security information protection are also demonstrated.
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-14
·
IF 3.9
Zhou Lei,Zhang Mengting,Sun Huinan,Duan Liyuan,Meng Qingwei
This study establishes the first reversed-phase HPLC-UV method for simultaneous baseline separation of 12 structurally diverse water-soluble vitamins (WSVs) within 25 min. The eco-friendly approach utilizes a methanol–potassium dihydrogen phosphate (pH 4.85) gradient on a ChromCore C18 column (250 × 4.6 mm, 5 μm) with dual-wavelength detection (210/266 nm), eliminating environmentally detrimental ion-pairing reagents. Rigorous validation per ICH Q2(R1) guidelines confirmed excellent linearity (R² > 0.999), precision (RSD < 2.1%), accuracy (spike recoveries: 93.9–106.8%), and sensitivity (LODs: 0.01–0.78 µg/mL). The method resolved key analytical challenges including polarity disparities, matrix interference, and vitamin instability. Successful quantification of endogenous vitamins in DMEM medium showed ≤ 5.1% deviation from certified values for 11/12 analytes. Applied to five commercial MDCK cell culture media, the method revealed significant inter-product variability in vitamin composition – notably universal under-supplementation of riboflavin (0.576–0.741 µg/mL, 10× lower than other vitamins) and inconsistent biotin/adenine supplementation. This robust, cost-effective platform provides biomanufacturers with a powerful quality control tool for optimizing vitamin supplementation in vaccine production systems, addressing a critical gap in cell culture analytics.
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危险品化学品经营许可证（带存储）