PHYTOMEDICINE
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2024
·
IF 6.7
Fei Qian,Bingchen Ouyang,Zuhuan Cai,Dan Zhu,Simiao yu,Jingcheng Zhao,Naijie Wei,Guangji Wang,Lin Wang,Jingwei Zhang
ABSTRACT Background Nonalcoholic fatty liver disease (NAFLD) is a common chronic liver disease with few therapeutic options currently available. Traditional Chinese medicine has been used for thousands of years and exhibited remarkable advantages against such complicated disease for its “multi-component, multi-target and multi-pathway” characteristics. Compound Shouwu Jiangzhi Granule (CSJG) is a clinical empirical prescription for the treatment of NAFLD, but its pharmacological mechanism remains unknown. Methods The clinical efficacy of CSJG was retrospectively analyzed in NAFLD patients by comparing blood biomarkers levels and liver MR images before and after CSJG treatment. Then, high-fat/high-fructose (HFHF) diet-induced NAFLD mice were used to further confirm CSJG's effect against hepatic lipid accumulation through hepatic lipid determination and histopathological staining of liver samples. Next, the ingredients of CSJG were determined, and network pharmacology analysis was performed to predict potential targets of CSJG, followed by quantitative PCR (qPCR) and western blotting for verification. Then, lipidomics study was carried out to further explore the anti-NAFLD mechanism of CSJG from the perspective of triacylglyceride (TAG) synthesis but not free fatty acid (FFA) synthesis. The enzymes involved in this process were assayed by qPCR and western blotting. The potential interactions between the key enzymes of TAG synthesis and the active ingredients of CSJG were analyzed by molecular docking. Results CSJG attenuated blood lipid levels and hepatic fat accumulation in both NAFLD patients and mice. Although network pharmacology analysis revealed the FFA synthesis pathway, CSJG only slightly affected it. Through lipidomics analysis, GSJG was found to significantly block the synthesis of diglycerides (DAGs) and TAGs in the liver, with decreased DGAT2 and increased PLD1 protein expression, which diverted DAGs from the synthesis of TAGs to the production of PEs, PCs and PAs and thus lowed TAGs level. Molecular docking suggested that rhein, luteolin and liquiritigenin from CSJG might be involved in this regulation. Conclusion Clinical and experimental evidence demonstrated that CSJG is a promising agent for the treatment of NAFLD. CSJG regulated TAGs synthesis to alleviate hepatic lipid accumulation. Rhein, luteolin and liquiritigenin from CSJG might play a role in it.
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APPLIED ORGANOMETALLIC CHEMISTRY
·
2025
·Vol. 39
·期 2
·e7957
·
IF 0
Yuzhang Yang,Minji Li,Yunqian Gong,Xingliang Li,Jia Zhou,Qinping Wei,Junke Zhang
Enzyme mimics attract wide attentions as detection sensors. However, the widespread application is limited by low efficiency and high cost. Herein, two novel enzyme mimics 1 and 2 were developed with peroxidase-like activities. The chain-like structure of 1 exhibited better stability, energy transformation efficiency, and catalytic property than 2 . Both of them could generate •OH radicals to oxide chromogenic agents, realizing the colorimetric detection of H 2 O 2 . Additionally, under the synergy of glucose oxidase, a cascade strategy for detection of glucose was constructed. This method exhibited excellent selectivity, anti-interference, and storage performance, as well as a wide linear range (1–60 μM) at pH = 7.4, with the low detection limits of 0.095 and 0.061 μM for 1 and 2 , respectively. Furthermore, the strategy was applied for detecting glucose in apple fruit samples. This work overcomes the shortcomings of current enzyme mimics and paves the way for bioanalysis and agricultural applications.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Liling Wang,Yanbin Wang,Yifeng Zhou
Bimetallic MOF derivatives have shown excellent performance as nano-enzymes in the field of catalysis. Herein, PdCo oxide nanoflowers with three-dimensional flower were prepared by a simple pyrolysis method on a precursor of bimetallic PdCo-MOF. PdCoO x showed excellent peroxidase mimic activity, which could significantly promote the oxidation of TMB by H 2 O 2 . Compared with CoO x , the peroxidase mimic activity of the optimized PdCoO x -300 increased by 2.41-fold. PdCoO x -300 has high affinity for TMB and H 2 O 2 with K m values of 0.16 mM and 2.11 mM, which are only 57.03% and 36.87% of HRP, respectively. The highly specific peroxidase mimic activity is conducive to the sensitive detection of H 2 O 2 , glucose and ascorbic acid with limit of detection of 10, 100 and 10 nM, respectively. Furthermore, the total antioxidant capacity in the actual beverage samples was conducted, which showed good anti-interference ability and recovery rate.
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CHEMICAL PHYSICS
·
2024
·
IF 2
Zhifeng Cai,Qi Liang,Yutong Wang,Xiao Wang,Lulu Jing,Yajie Cheng,Jinqi Liu,Zixuan Jia
Bright, blue fluorescent carbon quantum dots ( N -CDs) were carried out utilizing phenylenediamine and ascorbic acid as raw precursor through the hydrothermal method. The as-designed N -CDs suggested excitation-dependent characteristic changing from 340 nm to 380 nm. Moreover, Folic acid could availably quench the fluorescence intensity of the N -CDs. According to this experimental phenomenon, the sensitivity and selectivity of N -CDs for folic acid detection were developed. Under optimal experimental conditions, the as-prepared probe displayed excellent linearity with the folic acid concentration changing from 0.5 to 200 μM, and the limit of detection was 0.079 μM. Furthermore, the as-prepared probe indicated good sensitivity towards folic acid sensing in glucose and bovine serum samples. The recoveries of 95.9–104.8 % and 98.6–106.9 % were obtained in glucose and bovine serum samples, respectively. The corresponding relative standard deviation was lower than 2.74 % and 2.91 %, respectively. With preeminent sensitivity and selectivity, this as-developed nanosensor afforded a prospective platform for measuring folic acid in real samples. Furthermore, this N -CDs could be applied for the temperature detection.
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ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Qiumu Lin,Xiaoge Ji,Shijing Sun,Zhongyuan Zhao
Driven by sustainable development goals, the wood-based panel industry increasingly favors renewable biomass-based and formaldehyde-free adhesives. Saccharides, owing to their renewable environmental friendliness and high reactivity, have garnered attention for green adhesive development. However, existing saccharide-based adhesives face challenges due to demanding curing conditions (hot-pressing temperature ≥160 °C), limiting their industrial application. In this study, a fructose and toluene-4-sulfonic acid adhesive (FT adhesive) was prepared by a simple one-pot process. FT adhesive can be hot-pressed at 110 °C to prepare plywood in line with the China National Standard GB/T 9846–2015 (wet strength ≥0.7 MPa). Compared with other saccharide-based adhesives, FT adhesives show superior curing efficiency while maintaining high bonding strength. During the synthesis phase, fructose reacts with toluene-4-sulfonic acid at lower temperatures to produce various active compounds, including 5-HMF and its derivatives, formic acid, acetic acid, and furan compounds. These compounds undergo condensation reactions during high-temperature curing, forming polyfurans, humin polymers, and other insoluble polymers predominantly bonded by C–O–C, C–O, and C═O linkages. Additionally, toluene-4-sulfonic acid acts as a catalyst and an enhancer in both synthesis and curing phases, facilitating the conversion and cross-linking of 5-HMF and its derivatives. This process results in a robust and dense network structure, significantly enhancing the adhesive performance of FT adhesives at lower curing temperatures (110–130 °C). This research offers insights into the development of efficient and environmentally friendly saccharide-adhesives for wood-based panels.
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MICROCHIMICA ACTA
·
2012
·Vol. 177
·期 3
·485-490
·
IF 3.033
Luo Jing,Zhang Hongyan,Jiang Sisi,Jiang Jinqiang,Liu Xiaoya
We have developed a non-enzymatic glucose sensor by using a composite prepared from copper nanoparticles (CuNPs) and graphene which can be prepared by simple 1-step electrochemical reduction using graphene oxide (GO) and copper ion as the starting materials. The GO is electrochemically reduced to graphene at a voltage of −1.5 V, and this is accompanied by the simultaneous formation of CuNPs on the surface of the graphene. This novel nanocomposite combines the advantages of graphene and of CuNPs and displays good electrocatalytic activity toward glucose in alkaline media. The performance of the respective glucose electrode was evaluated by amperometric experiments and revealed a fast response (<2 s), a low detection limit (200 nM), and high sensitivity (607 μA mM −1 ). The sensor also exhibits good reproducibility and very good specificity for glucose over ascorbic acid, dopamine, uric acid, fructose, lactose and sucrose.
(A) CVs of Cu NPs/graphene electrode (a), graphene electrode (b),and Cu/GC electrode (c) in 0.1 M NaOH solution with 0.5 mM glucose; (B) The response of the Cu NPs/graphene electrode to successive addition of glucose from 5 μM to 0.2 mM.
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LUMINESCENCE
·
2024
·Vol. 39
·期 10
·e4912
·
IF 3.2
Congcong Hu,Wenjuan Wang,Qianwen Li,Bin Liu,Fubing Xiao,Jinquan Liu,Shengyuan Yang
Hence, N,S-CDs with photoluminescent property were simply synthesized via a one-step hydrothermal method. Combined with the commercial reagent Ce 4+ , a ratiometric fluorescence assay for ascorbic acid (AA) detection was established. Ce 4+ , possessing oxidization, could directly oxidize o-phenylenediamine (OPD) to form the yellow fluorescent product oxOPD. Under the excitation wavelength of 370 nm, oxOPD had a maximum fluorescence emission at 562 nm. Meanwhile, due to the occurrence of the inner filter effect (IFE), oxOPD quenched the fluorescence of N,S-CDs. However, ascorbic acid (AA) inhibited the oxidation of Ce 4+ , causing the fluorescence of oxOPD at 562 nm to decrease, accompanied by an increase in the fluorescence belonging to N,S-CDs at 450 nm. Thus, a Ce 4+ -assisted ratiometric fluorescence method was established for AA detection. The two fluorescence output signals in this method had opposite changing trends, which could reduce system errors and improve the accuracy. This method was successfully applied to the determination of AA in drugs and fruits.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Mingfu Li,Liyuan Huai,Yingchuan Zhang,Hongli Ma,Pingjun Zhang,Feixiang Xu,Jian Zhang,Liqun Jiang
The green synthesis of 5-hydroxymethylfurfural (HMF) from biomass feedstocks is a crucial step for the development of sustainable fuels, resins and plastics. Here, a novel binary catalytic system was developed with metal–organic frameworks (MOFs) and deep eutectic solvents (DESs), which synergically exhibited a superior fructose dehydration efficiency with a HMF yield of 98.5% within 40 min. The replacement from terephthalic acid to 2,5-furandicarboxylic acid as the organic ligand in MOFs catalysts altered the coordination microenvironment of metal nodes, resulting in remarkably enhanced fructose conversion rates. The formation of hierarchical porous structures and moderated Brønsted acid sites in sulfonated MOFs further promoted the HMF production. Notably, HMF could be readily separated from the MOFs-DESs system and the MOF catalyst maintained stable performance after six recycles. Theoretical calculations clarified a positive correlation between the hydrogen bond interaction (between fructose and DESs components) energy and reaction efficiency. This study provided a new strategy to couple hierarchical solid acid catalysts with functional solvents binary towards effective and sustainable upgrading of biomass-derived molecules.
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CHEMICAL PHYSICS
·
2024
·
IF 2
Ronghua Zhang,Shikun He,Fei Wang,Bo Cai,Junfeng Feng,Hui Pan
Porous bi-functional catalsyts consisting of metals and zeolites are used as solid acid catalysts, characterized by octahedral morphology and good stability. In this work, a series of bifunctional catalysts (xZr-HY) with Lewis and Brønsted acid sites were prepared by a simple impregnation method, and used for the conversion of glucose to levulinic acid and methyl levulinate. The catalysts were characterized using different methods of physical and chemical properties. The analysis results showed that these catalysts existed in tetrahedral form with good pore structure , suitable acid strength and acidity. Using 10Zr-HY as the catalyst, 43.0 % yield of LA and ML could be obtained at 200 ℃ for 2.5 h. In addition, this catalytic system also gave high LA and ML yields from other biomass carbohydrates. Meanwhile, the 10Zr-HY catalyst had good stability and reusability after four cycles. This catalyst has the potential to convert biomass into high value-added platform compounds.
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ANALYTICAL CHEMISTRY
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2024
·
IF 6.7
Shen Jiang,Qiuyun Li,Guangrun Wu,Xuming Mu,Xiaotong Wang,Yunpeng Wang,Yanli Wu,Jing Wu,Yang Li
In the landscape of biomolecular detection, surface-enhanced Raman spectroscopy (SERS) confronts notable obstacles, particularly in the label-free detection of biomolecules, with glucose and other sugars presenting a quintessential challenge. This study heralds the development of a pioneering SERS substrate, ingeniously engineered through the self-assembly of nanoparticles of diverse sizes (Ag1@Ag2NPs). This configuration strategically induces ‘hot spots’ within the interstices of nanoparticles, markedly amplifying the detection signal. Rigorous experimental investigations affirm the platform’s rapidity, precision, and reproducibility, and the detection limit of this detection method is calculated to be 6.62 pM. Crucially, this methodology facilitates nondestructive glucose detection in simulated samples, including phosphate-buffered saline and urine. Integrating machine learning algorithms with simulated serum samples, the approach adeptly discriminates between hypoglycemic, normoglycemic, and hyperglycemic states. Moreover, the platform’s versatility extends to the detection and differentiation of monosaccharides, disaccharides, and methylated glycosides, underscoring its universality and specificity. Comparative Raman spectroscopic analysis of various carbohydrate structures elucidates the unique SERS characteristics pertinent to these molecules. This research signifies a major advance in nonchemical, label-free glucose determination with enhanced sensitivity via SERS, laying a new foundation for its application in precision medicine and advancing structural analysis in the sugar domain.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Siyao Zhang,Shiyou Yu,Xin Wang,Yipeng Zhang,Zhenge Yue,Chenchen Li,Yongqiang Ma
During beekeeping, the utilization of metronidazole (MNZ) for treating bee ailments leads to the MNZ residues in honey. Prolonged ingestion of honey with MNZ residues could pose potential health hazards to humans. Therefore, the development of a straightforward and efficient MNZ detection method is of utmost importance. ZnO, a non-toxic catalytic material, exhibits promising potential in electrochemical sensor design. The resulting g-C 3 N 4 /MnO 2 /ZnO composite was employed to craft an electrochemical sensor that facilitates swift MNZ quantification in honey. Comprehensive characterization of the synthesized composites was performed, and the electrochemical behavior of MNZ was explored using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). It was found that the g-C 3 N 4 /MnO 2 /ZnO composite exhibits remarkable electrocatalytic capability, and the resulted sensor demonstrates good sensitivity. Within a range of 2–250 μM, MNZ's current value showed two linear relationships with its concentration, achieving the detection limit of 0.21 μM. When applied to honey samples, the g-C 3 N 4 /MnO 2 /ZnO/GCE sensor detected MNZ with recovery rates spanning 93.7% to 100.3% and relative standard deviations (RSD) under 3%. This attributed to heightened sensitivity, selectivity, repeatability and durability of the sensor. Furthermore, its effectiveness extends to the rapid assessment of MNZ content in actual honey, highlighting its practical utility in identifying MNZ residues in honey.
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Nanomaterials
·
2024
·Vol. 15
·期 1
·47
·
IF 4.4
Yueyun Huang,Jiahua You,Yingru Ding,Yun Xie,Ting Wang,Fanglong Zhu,Weiping Gong,Zhenting Zhao
Non-enzymatic glucose detection is an effective strategy to control the blood glucose level of diabetic patients. A novel hierarchical core–shell structure of nickel hydroxide shell coated copper hydroxide core based on copper foam (Ni(OH)2@Cu(OH)2-CF) was fabricated and derived from NiO@Cu2O-CF for glucose sensing. Cyclic voltammetry and amperometry experiments have demonstrated the efficient electrochemical catalysis of glucose under alkaline conditions. The measurement displays that the fabricated sensor exhibits a detection scale of 0.005–4.5 mM with a detection sensitivity of 4.67 µA/µM/cm2. It has remarkable response/recovery times in respect of 750 μM glucose (1.0 s/3.5 s). Moreover, the NiO@Cu2O-CF shows significant selectivity, reliable reproducibility and long-term stability for glucose determination, suggesting it is a suitable candidate for further applications.
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