Communications Biology
·
2024
·Vol. 7
·期 1
·1-17
·
IF 5.2
Wang Yi,Fu Xiaolong,Zeng Li,Hu Yan,Gao Rongyang,Xian Siting,Liao Songjie,Huang Jianxiang,Yang Yonggang,Liu Jilong,Jin Hai,Klaunig James,Lu Yuanfu,Zhou Shaoyu
Nuclear factor erythroid 2-related factor-2 (Nrf2) antioxidant signaling is involved in liver protection, but this generalization overlooks conflicting studies indicating that Nrf2 effects are not necessarily hepatoprotective. The role of Nrf2/heme oxygenase-1 (HO-1) in cholestatic liver injury (CLI) remains poorly defined. Here, we report that Nrf2/HO-1 activation exacerbates liver injury rather than exerting a protective effect in CLI. Inhibiting HO-1 or ameliorating bilirubin transport alleviates liver injury in CLI models. Nrf2 knockout confers hepatoprotection in CLI mice, whereas in non-CLI mice, Nrf2 knockout aggravates liver damage. In the CLI setting, oxidative stress activates Nrf2/HO-1, leads to bilirubin accumulation, and impairs mitochondrial function. High levels of bilirubin reciprocally upregulate the activation of Nrf2 and HO-1, while antioxidant and mitochondrial-targeted SOD2 overexpression attenuate bilirubin toxicity. The expression of Nrf2 and HO-1 is elevated in serum of patients with CLI. These results reveal an unrecognized function of Nrf2 signaling in exacerbating liver injury in cholestatic disease.
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RESEARCH ON CHEMICAL INTERMEDIATES
·
2024
·
IF 2.8
Xiong Neng,Jiang Lin-Li,Chen Jia-Yu,Lin Lei,Huang Jin-Rong,Shen Qi,Xue Ya-Ping,Zheng Yu-Guo
Hydrogenation plays an important role in the production of bulk and fine chemicals. In the present work, the hydrogenation process of 1-cyanocyclohexaneacetic acid (1-CA), a key intermediate in the chemo-enzymatic synthesis of the antiepileptic drug gabapentin, was investigated. The catalysts were screened, the operating parameters for the 1-CA hydrogenation were determined by response surface methodology, and the effects of biological impurities on the reaction and catalysts were investigated. The reaction temperature, reaction solution pH and reaction time were identified as the main factors for hydrogenation by single-factor experiments. Under the optimal reaction conditions: reaction temperature 110 °C, solution pH 12.33, reaction time 3.74 h, hydrogen pressure 2 MPa and stirring speed 500 rpm, the substrate conversion reached 100%, and the total product yield reached 96.40%, which was very close to the prediction (96.88%). It was demonstrated that biological impurities greatly affect the reaction rate of hydrogenation, as well as the activity and reusability of the catalyst, in which protein impurities lead to catalyst deactivation through cumulative deposition on the catalyst surface and within the pores.
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INORGANIC CHEMISTRY
·
2024
·
IF 4.3
Jing-ran Yu,Pan-pan Zhang,Jia-yu Ding,Yue-bin Liu,Wei-hai Lin,Wei Shao,Jia-jia He,Qihan Gong,Ming Xue
The separation of high-octane dibranched alkanes from naphtha is critical in the refining of gasoline. To date, research on the membrane-based separation of alkane isomers has been limited, with a particular paucity of investigations into mixed-matrix membranes. Herein, the continuous and dense UiO-66/PIM-1 mixed-matrix membrane, which was prepared through precise control of the interfacial structure, was first applied to the differentiation of C6 alkane isomers. Due to the synergistic combination of UiO-66 with differential adsorption capabilities for alkanes and PIM-1 that possesses a cross-linkable structure, the resulting UiO-66/PIM-1-(20) membrane demonstrated remarkable separation performance and high stability. Pervaporation measurements showed that the mass fraction of 2,2-dimethylbutane in the feed side was increased from 50.0 to 75.8 wt % while an excellent flux of 1700 g m–2 h–1 was maintained over a continuous 40 h period. The UiO-66/PIM-1-(20) membrane, characterized by its facile replication and processing, shows potential for large-scale fabrication. This study offers a new approach to the membrane separation of alkane isomers.
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SusMat
·
2025
·
IF 21.3
Xuechun Hu,Yuchen Pang,Chaoqun Li,Xiaoyue Zhang,Xuebin Yu,Fang Fang,Guanglin Xia
Magnesium hydride (MgH 2 ) has been regarded as an attractive candidate for solid-state hydrogen storage, yet its practical applications are limited by the requirement of elevated temperatures and sluggish hydrogen uptake and release kinetics. Herein, TiO 2 polyhedral frameworks with uniformly distributed V 2 O 5 (denoted as V 2 O 5 /TiO 2 ) are constructed to improve hydrogen storage performance of Mg/MgH 2 . During the reversible hydrogenation and dehydrogenation process, metallic V and Ti along with low-valent Ti- and V-based oxides are in situ formed. Among them, metallic V supported on TiO 2 exhibits the lowest hydrogen adsorption energy, enabling superior catalytic performance over TiO 2 and V 2 O 5 . As a result, the peak dehydrogenation temperature of MgH 2 decreases to 215°C, 105°C lower than that of pristine MgH 2 , with a decrease of the apparent activation energy from 139.50 kJ·mol −1 to 68.99 kJ·mol −1 . Moreover, electron migration from V toward TiO 2 leads to charge accumulation around Ti and O atoms, shifting the V 3 d -band center toward the Fermi level and thereby improving the catalytic function of V's d-electrons, facilitating hydrogen dissociation without energy barriers. Therefore, the V 2 O 5 /TiO 2 -catalyzed Mg absorbs 4.12 wt% H 2 under an ultralow pressure of 1 bar at 25°C. This provides a new strategy for developing advanced Ti and V-based catalysts for mild-condition hydrogen storage of MgH 2 .
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Jingyi Chen,Xuan Wang,Xuemei Yuan,Haiting Zou,Jiaye Li,Guopu Chen,Qian Tan
Fibroblast dysfunction is a key factor in the prolonged healing of diabetic wounds. Netrin-1 was found to be able to ameliorate high-glucose-induced fibroblast dysfunction. In this study, we developed a biomimetic dual-network hydrogel loaded with Netrin-1 to promote diabetic wound healing. The hydrogel was composed of natural ingredients, sodium alginate (SA) and glycyrrhizic acid (GA). In addition, with the octopus’s suction cup structure on the surface, this hydrogel exhibited strong adhesion properties and provided protective and sustained release of Netrin-1. The released could promote autophagy by activating the AMPK-mTOR pathway under sustained hyperglycemic conditions, thereby reversing fibroblast dysfunction and restoring cell migration and collagen synthesis. The resultant SA/GA@Netrin-1 hydrogel not only possessed potent antioxidant and antimicrobial effects but also enhanced collagen deposition in diabetic rat wounds, attenuated the inflammatory response, promoted autophagy, and significantly accelerated wound closure. These findings suggest that the SA/GA@Netrin-1 hydrogel provided an innovative solution for chronic diabetic wound management and has the potential to be applied to other refractory wound therapies.
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VETERINARY MICROBIOLOGY
·
2025
·
IF 2.7
Qing Yang,Shengda Xie,Yundi Zhao,Xinxin Lin,Ning Wei,Miaolei Shi,Ruibing Cao
Japanese encephalitis virus (JEV) is a zoonotic pathogen that can be transmitted to humans and animals via arthropods, causing viral encephalitis. In this study, we investigated the role of transferrin receptor 1 (TfR1) in JEV infection. We found that knocking down TfR1 expression in A549 and HeLa cells significantly inhibited JEV entry, while overexpression of TfR1 in 293 T cells enhanced viral entry. The JEV E protein interacts with TfR1, specifically via the protease-like domain (residues 384–606). Additionally, the hTfR1 soluble ectodomain protein significantly blocked JEV infection in HeLa cells, and ferric ammonium citrate (FAC), an iron supplement, downregulated TfR1 expression and effectively inhibited JEV entry and infection. These findings identify TfR1 as an entry factor for JEV and a potential antiviral target, suggesting that small molecule inhibitors like FAC could offer a promising antiviral strategy.
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MOLECULES
·
2022
·Vol. 27
·期 14
·4466
·
IF 4.927
Xue Li,Jun Wu,Fan Fang,Hongping Li,Lei Wang,Hui Wan,Guofeng Guan
Based on the acid–base neutralization, the (1-methylimidazolium)(tetrazol-1-yl)borane was successfully synthesized by taking advantage of the acidity of the tetrazole and the basicity of the 1-methylimidazole borane complex. Through HRMS, NMR, and FT−IR, the structure of synthetic compounds was characterized in detail. Concerning about the (1-methylimidazolium)(tetrazol-1-yl)borane, it had an ignition−delay time of about 25 ms and a density specific impulse over 351 s·g/cm3, making it a suitable candidate for green hypergolic fuels. Moreover, it also demonstrated that introducing tetrazole into the borane could be an appropriate strategy to adjust the performance of the energy of those borane compounds.Keywords:borane;azole;hypergolic fuel;acid–base neutralization
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GEOMICROBIOLOGY JOURNAL
·
2015
·
IF 1.44
Hongliang Liu,Jun Huang,Shengzhe Zhang,Biao Xu,Gejiao Wang
This study was conducted to determine the microbe-chromate [Cr(VI)] interaction and the effect of quinoid analogue anthraquinone-2-sulfonate (AQS) on aerobic Cr(VI) reduction by Intrasporangium chromatireducens Q5-1. The addition of redox mediator AQS, which might expedite the electron transfer, promoted Cr(VI) bioreduction. Addition of carbon sources, such as maltose, acetate, sucrose and lactose stimulated the AQS-promoted Cr(VI) reduction of strain Q5-1. Induction experiment clarified that the enzyme involves in the Cr(VI) reduction is constitutive. Energy-dispersive spectroscopy (EDS) spectra showed the existence of trace Cr distributed on the cell surface. X-ray photoelectron spectroscopy (XPS) analysis revealed that the Cr(III) complex was bound to the cell surface (0.87%, atomic percent). The spectra shifts detected by Fourier transform infrared (FTIR) spectroscopy indicated that Cr(III) was bound to the carbonyl and amide groups. In addition, Cr(VI) reduction by different cell fractions showed that Cr(VI) reduction was occurred extracellularly rather than intracellularly. The results disclosed that Cr(VI) detoxification of strain Q5-1 was mainly associated with extracellular Cr(VI) reduction process in combination with trace Cr(III) adsorption on the cell surface. A schematic figure depicting the interactions between strain Q5-1 and Cr(VI) was presented. This study enhanced the understanding of the microbe-Cr(VI) interaction mechanism and revealed the AQS-promoted aerobic Cr(VI) reduction of strain Q5-1. Such strain and quinoid analogue-mediated bacterial Cr(VI) reduction may facilitate the bioremediation for Cr(VI)-polluted environment.
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COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
·
2022
·Vol. 25
·期 9
·1545-1551
·
IF 1.714
Chen Genqiang,Zhu Lina,He Jiaxuan,Zhang Song,Li Yuanhao,Guo Xiaolong,Sun Di,Tian Yuee,Liu Shengming,Huang Xiaobo,Che Zhiping
Background: Developing the high-efficiency and low-risk small-molecule greenfungicide is the key to effective control of the plant pathogenic oomycetes. Essential oils play a very important role in novel fungicide discovery for their unique sources and potential target sites. Eugenol,
a kind of plant essential oil, was mainly isolated from the unopened and dried flower buds of Syzygium aromaticum of the Myrtaceae family. Due to its unique structural skeleton, eugenol and its derivatives have exhibited a wide range of biological activities. However, a study on the synthesis
of novel 1-sulfonyloxy/acyloxyeugenol derivatives as fungicidal agents against Phytophthora capsici has not yet been reported. Methods: Twenty-six novel 1-sulfonyloxy/acyloxyeugenol derivatives (3a-p and 5a-j) were prepared and their structures were well characterized by 1H
NMR, HRMS, and m.p. Their fungicidal activity was evaluated against P. capsici by using the mycelial growth rate method. Results: To find novel natural-product-based fungicidal agents to control the plant pathogenic oomycetes, we herein designed and synthesized two series of novel 1-sulfonyloxy/acyloxyeugenol
derivatives (3a-p and 5a-j) as fungicidal agents against P. capsici Leonian, in vitro. Results of fungicidal activity revealed that, among all compounds, especially compounds 3a, 3f, and 3n displayed the most potent anti-oomycete activity against P. capsici with EC50 values of 79.05, 75.05,
and 70.80, respectively. Conclusion: The results revealed that the anti-oomycete activity of eugenol with the sulfonyloxy group was higher than that with the acyloxy group. It is suggested that the fungicidal activity of eugenol can be improved by introducing the sulfonyloxy group. This
will pave the way for further design, structural modification, and development of eugenol derivatives as fungicidal agents.
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Microbial Cell Factories
·
2021
·Vol. 20
·期 1
·1-11
·
IF 5.328
Wan Yupeng,Liu Hongchen,Xian Mo,Huang Wei
Background 1-Hydroxyphenazine (1-OH-PHZ) is a phenazine microbial metabolite with broad-spectrum antibacterial activities against a lot of plant pathogens. However, its use is hampered by the low yield all along. Metabolic engineering of microorganisms is an increasingly powerful method for the production of valuable organisms at high levels. Pseudomonas chlororaphis is recognized as a safe and effective plant rhizosphere growth-promoting bacterium, and faster growth rate using glycerol or glucose as a renewable carbon source. Therefore, Pseudomonas chlororaphis is particularly suitable as the chassis cell for the modification and engineering of phenazines. Results In this study, enzyme PhzS (monooxygenase) was heterologously expressed in a phenazine-1-carboxylic acid (PCA) generating strain Pseudomonas chlororaphis H18, and 1-hydroxyphenazine was isolated, characterized in the genetically modified strain. Next, the yield of 1-hydroxyphenazine was systematically engineered by the strategies including (1) semi-rational design remodeling of crucial protein PhzS, (2) blocking intermediate PCA consumption branch pathway, (3) enhancing the precursor pool, (4) engineering regulatory genes, etc. Finally, the titer of 1-hydroxyphenazine reached 3.6 g/L in 5 L fermenter in 54 h. Conclusions The 1-OH-PHZ production of Pseudomonas chlororaphis H18 was greatly improved through systematically engineering strategies, which is the highest, reported to date. This work provides a promising platform for 1-hydroxyphenazine engineering and production. Graphical Abstract
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2019
·Vol. 30
·期 7
·6760-6767
·
IF 2.195
Bao Runxi,Li Mingyu,Shen Meng,Liu Huan,Zhang Guangzu,Zeng Yike,Jiang Shenglin
Ceramic-polymer pyroelectric composite materials with favorable flexibility hold great potential for wearable sensors and energy harvesters, and the further improvement in pyroelectric properties would push them closer to practical application. In this work, an uniaxial stretching method was proposed to optimize the performance of lead zirconate titanate nanowires (PZT NWs)/polyvinylidene fluoride (PVDF) 1–3 composites. With just one stretching treatment, the pyroelectric coefficient of the composite was enhanced by inducing more β phase in PVDF matrix, and the permittivity was decreased by reorientation of PZT NWs to the strain direction in the meantime. As a result of the incorporated effects, the figure of merit of the stretched composite with orientated PZT NWs increases to the value of 2.18 × 10 −10 C cm −2 K −1 , which is 619% higher than that of untreated samples at 35 °C. This work could provide an efficient approach to fabricate high-performance ceramic-polymer pyroelectric composite materials.
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AMERICAN JOURNAL OF THE MEDICAL SCIENCES
·
2022
·
IF 3.462
Qi Wang,Yangfan Li,Chuanqiang Wu,Tong Wang,Ming Wu
Background Ischemia-reperfusion injury (IRI), which involves severe inflammation and edema, is an inevitable feature of the lung transplantation process and leads to primary graft dysfunction (PGD). The activation of aquaporin 1 (AQP1) modulates fluid transport in the alveolar space. The current study investigated the role of AQP1 in ischemia-reperfusion (IR)-induced lung injury. Methods A mouse model of lung IR was established by clamping the left lung hilar for 1 h and released for reperfusion for 24 h. The AQP1 inhibitor acetazolamide (AZA) was administered 3 days before lung ischemia with a dose of 100 mg/kg per day via gavage. Lung injury was evaluated using the ratio of wet-to-dry weight, peripheral bronchial epithelial thickness, degree of angioedema , acute lung injury score, neutrophil infiltration , and cytokine concentrations in bronchoalveolar lavage fluid . Results Compared with sham treatment , ischemia with no reperfusion (IR 0h) and ischemia with reperfusion for 24 h (IR 24 h) significantly upregulated AQP1 expression, increased the wet/dry weight ratio, angioedema, neutrophil infiltration and cytokine production (interleukin -6 and tumor necrosis factor -α) and thickened the peripheral bronchial epithelium . AZA exacerbated inflammation and pulmonary edema. Conclusion AQP1 may exert a protective effect against IR-induced lung injury, which could be attributed to alleviating pulmonary edema and inflammation. AQP1 upregulation might be a potential application to alleviate lung IRI and decrease the incidence of PGD.
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