Biochemistry and Cell Biology
·
2022
·Vol. 101
·期 1
·
IF 3.73
Guihua Song,Suping Yu,Yan Zhang,Mengmeng Sun,Bingxue Zhang,Minghao Peng
Asthma is characterized by airway inflammation and remodeling. 2-Undecanone (methyl nonyl ketone), a volatile organic compound originating from Houttuynia cordata, has the potential to ameliorate inflammatory diseases. This study aimed to explore potential benefits of 2-undecanone in asthma. 2-Undecanone (100, 200, or 400 mg/kg) was administered intragastrically to ovalbumin (OVA)-challenged BALB/c mice. Lung tissues were collected to observe histopathological changes, and bronchoalveolar lavage fluid (BALF) was collected for the detection of inflammatory cells and cytokine production. The results showed that 2-undecanone ameliorated OVA-induced pathologic changes of lungs, including reducing inflammatory cell infiltration, goblet cell hyperplasia, and airway smooth muscle thickness. The number of inflammatory cells and the levels of IL-4, IL-5, IL-13, and IgE in BALF were decreased by 2-undecanone in asthmatic mice. Furthermore, abnormal activation of NF-κB pathway in lung tissues of asthmatic mice was impeded by 2-undecanone. In vitro, 2-undecanone (12.5, 25, or 50 µM) suppressed platelet-derived growth factor-BB-induced proliferation and migration of primary airway smooth muscle cells (ASMCs), and inhibited the switching of ASMCs from contractile phenotype to synthetic phenotype. Consistently, 2-undecanone blocked NF-κB activation in ASMCs. Collectively, 2-undecanone relieves asthma through alleviating airway inflammation and remodeling, and this beneficial effect is achieved by inhibiting NF-κB pathway.
研究领域:
免疫学
·
药理学
·
呼吸医学
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF CATALYSIS
·
2020
·
IF 6.146
Zhou Ren,Yang Liu,Yuan Lyu,Xiangen Song,Changyong Zheng,Zheng Jiang,Yunjie Ding
A single-Rh-site catalyst (Rh-TPISP) that was ionically-embedded on a P(V) quaternary phosphonium porous polymer was evaluated for heterogeneous ethanol carbonylation . The [Rh(CO)I 3 ] 2– unit was proposed to be the active center of Rh-TPISP for the carbonylation reaction based on detailed Rh L 3 -edge X-ray absorption near edge structure (XANES), X-ray photoelectron spectroscopy (XPS), and Rh extended X-ray absorption fine structure (EXAFS) analyses. As the highlight of this study, Rh-TPISP displayed distinctly higher activity for heterogeneous ethanol carbonylation than the reported catalytic systems in which [Rh(CO) 2 I 2 ] − is the traditional active center. A TOF of 350 h −1 was obtained for the reaction over [Rh(CO)I 3 ] 2– , with >95% propionyl selectivity at 3.5 MPa and 468 K. No deactivation was detected during a near 1000 h running test. The more electron-rich Rh center was thought to be crucial for explaining the superior activity and selectivity of Rh-TPISP, and the formation of two ionic bonds between [Rh(CO)I 3 ] 2– and the cationic P(V) framework ([P] + ) of the polymer was suggested to play a key role in firmly immobilizing the active species to prevent Rh leaching.
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产品关联
DOI
高影响因子
INTERNATIONAL DENTAL JOURNAL
·
2025
·Vol. 76
·期 1
·109304
·
IF 3.7
Zhaoxia Yu,Shihui Jiang,Xinxin Li,Xingjiao Li,Guanhua Wang,Xiaohua Dai,Xiaoli Lian,Yan Yan,Yue Wang,Zhimou Yang,Huiru Zou
Objectives Enamel matrix derivative (EMD) is widely recognized for its ability to induce osteogenic and odontogenic differentiation. However, the complex composition of EMD frequently results to inconsistent experimental and clinical outcomes. Amelogenin, a key component of EMD, plays crucial roles in tooth germ development as well as in the repair and regeneration of dental tissue. In this study, an amelogenin peptide was investigated on the proliferation and odontogenic differentiation of human dental pulp cells (HDPCs). Methods The amelogenin peptide with the amino acid sequence KWYQNMIR was synthesized using solid-phase techniques. HDPCs were stimulated with various concentrations of the peptide. CCK-8 assays, alkaline phosphatase (ALP) activity measurements, quantitative reverse transcription-polymerase chain reaction, Western blot analysis, Alizarin red staining, and scanning electron microscopy were used to assess the effects. Results The amelogenin peptide concentrations of 100 nM and 1 μM significantly enhanced HDPC proliferation at 5 and 7 days ( P < .01), with 1 μM exhibiting the most pronounced effect. Treatment with 1 μM amelogenin peptide enhanced ALP activity in HDPCs at 7 and 14 days ( P < .01) and markedly upregulated the mRNA expression of DSPP, DMP-1 , and RUNX-2 . Western blot analysis revealed that 1 μM amelogenin peptide enhanced the protein expression of DSPP, DMP-1, and ALP in HDPCs at 7 and 14 days. Additionally, it promoted calcium deposition and mineralized nodule formation at 28 days. Importantly, the amelogenin peptide upregulated the phosphorylation level of ERK1/2 (p-ERK1/2), and the application of an ERK1/2 inhibitor suppressed HDPC differentiation. Conclusions The amelogenin peptide stimulates both the proliferation and odontogenic differentiation of HDPCs via the ERK1/2 pathway. These findings suggest that the amelogenin peptide may have significant implications for regenerative dentistry, particularly in the context of dentine-pulp complex regeneration.
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产品关联
DOI
PubMed
高影响因子
DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 31
·10918-10926
·
IF 4
Yu Zhang,Xia Liu,Qian-Yan Liu,Jian-Hua Wang,Ting Hu,Yan-Mei Lin,Jian-Han Zhang
Two novel alkaline-earth zincophosphites, namely CaZn(HPO3)2 and Ba2Zn(HPO3)3, were successfully synthesized under hydrothermal conditions. CaZn(HPO3)2 exhibits a three-dimensional (3D) anionic framework with a 6-connected uni-nodal pcu α-Po primitive cubic topology, constructed by unique Zn2O8 dimers and HPO3 pseudo-tetrahedra, while Ba2Zn(HPO3)3 displays one-dimensional (1D) [Zn(HPO3)3]4− anionic chains. It is worth mentioning that CaZn(HPO3)2 represents the first example of an alkaline-earth zincophosphite compound with a 3D framework structure. Our research also revealed the importance of both alkaline earth cation sizes and Zn/P ratios in anionic open-framework formation. The crystal structures of both compounds were further verified by energy dispersive spectroscopy, IR spectroscopy and Raman spectroscopy. Optical diffuse reflectance spectra, coupled with Tauc's fitting, revealed direct bandgaps with energy values of 4.33 and 4.48 eV for CaZn(HPO3)2 and Ba2Zn(HPO3)3, respectively, which differ from the prediction of theoretical calculations. Density of states calculations were conducted to reveal the origin of the bandgaps and bond interactions. Both compounds exhibited moderate birefringence values. This work may have implications for the design and synthesis of novel metal phosphites with desired properties.
研究领域:
无机化学
·
材料科学
·
固体化学
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DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2016
·
IF 3.15
Fubao Gong,Jun Shen,Runhua Gao,Xiong Xie,Xiong Luo
A novel organic–inorganic Mg(OH) 2 /silane surface layer has been developed for corrosion protection of AZ31 magnesium alloy. The results of electrochemical impedance spectroscopy (EIS), the immersion tests, Fourier-transform infrared spectroscopy (FTIR) and sellotape tests showed that the Mg(OH) 2 /silane-based composite surface layer possessed excellent corrosion resistance and very good adhesion due to the formation of Si–O–Mg bond between Mg(OH) 2 layer and silane layer. Electrochemical impedance spectroscopy tests results indicated that for the long-term corrosion protection of AZ31 the increase of the curing temperature improved the impedance of the composited layer when the curing temperature was lower than 130 °С. However, the impedance of the composited layer deceased when the curing temperature was more than 130 °С due to the carbonization of the silane layer.
研究领域:
材料科学
·
腐蚀与防护
✓ 已验证
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 97
·95199-95203
·
IF 3.289
Shi-Yu Lu,Sheng-Hui Liao,Shu-Juan Bao,Meng Jin,Bo Weng,Chang-Ming Li
The excellent selectivity and anti-interference ability of enzyme-based biosensors have attracted considerable attention by researchers. However, the short lifetime, difficult electron transfer, and easily leak from the supporting materials restrict the practical applications of enzyme. In this study, an enzyme–inorganic hybrid biosensor constructed from slack Hb–Mn3(PO4)2·3H2O nanosheets was prepared by a simple and effective in situ immobilization method. Designed as an H2O2 sensor, it exhibits a fast electron transfer rate constant (ks = 4.16 s−1), an ultra-wide linear range for H2O2 detection (20 to 56 100 μM), and long life-time (85% of initial response after 29 days), which may be attributed to the effective immobilization of the enzyme and the good biocompatibility of Mn3(PO4)2·3H2O. For the detection of a real sample, the range of recovery of our designed biosensor was around 98.2–102.6%, confirming that our proposed method presents a promising immobilization enzyme-based biosensor for use in practical applications.
研究领域:
生物化学
·
分析化学
·
材料科学
✓ 已验证
DOI
高影响因子
STAR Protocols
·
2022
·
IF 0
Jing Zhang,Haohao Yan,Gangan Yan,Xiaoping Liu,Yanchang Wang,Yunyu Chen
Summary Discovery of efficacious antiviral agents targeting SARS-CoV-2 main protease (Mpro) is of the highest importance to fight against COVID-19. Here, we describe a simple protocol for high-throughput screening of Mpro inhibitors using a robust fluorescence polarization (FP) assay. Candidate Mpro inhibitors from large compound libraries could be rapidly identified by monitoring the change of millipolarization unit value. This affordable FP assay can be modified to screen antiviral agents targeting virus protease. For complete details on the use and execution of this protocol, please refer to Li et al. (2022) , Yan et al. (2021) , and Yan et al. (2022c) .
研究领域:
病毒学
·
药学
·
分子生物学
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DOI
PubMed
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2020
·
IF 4.939
Yan Sang,Xi Cao,Lvxuan Wang,Gaofei Ding,Yingjie Wang,Deshuang Yu,Yanan Hao,Linlin Li,Shengjie Peng
Synthesis of highly efficient, non-noble and bi-functional electrocatalysts is exceedingly challenging and necessary for water splitting devices. In this work, three-dimensional spherical Ni(OH) 2 /NiCo 2 O 4 heterojunctions are prepared by a one-step hydrothermal method and the hybrids are explored as efficient electrocatalysts for oxygen evolution reaction (OER) and hydrogen evolution reaction (HER) in an alkaline electrolyte via tuning different Ni/Co atomic ratios of heterojunctions . The optimized Ni(OH) 2 /NiCo 2 O 4 (S (1:1)) exhibits high electrocatalytic activity with an ultralow over-potential of 189 mV at 10 mA cm −2 for the HER. With regard to the OER, the over-potential of the as-synthesized S (1:1) heterojunction is only 224 mV at the current density of 10 mA cm −2 . The improved catalytic performance of the Ni(OH) 2 /NiCo 2 O 4 heterojunctions is attributed to the chemical synergic combining of Ni(OH) 2 and NiCo 2 O 4 , large specific surface area for exposing more accessible active sites, and heterointerface for activating the intermediates that facilitates electron/electrolyte transport. The prepared catalyst exhibits good durability and stability in HER and OER catalyzing conditions. This study provides a feasible approach for the building of highly efficient bifunctional water splitting electrocatalysts and stimulates the development of renewable energy conversion and storage devices.
研究领域:
材料科学
·
能源化学
✓ 已验证
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Xintong Wang,Jiang Yang,Xu Chen,Wei Ding
The inhibition properties of 2-mercaptobenzimidazole (MBI) and its synergism with oleic imidazoline (OIM) for carbon steel in CO 2 -saturated brine solution were investigated by electrochemical, weight loss measurements, surface analysis and quantum chemical calculations. The effectiveness of MBI for OIM was also compared to the other sulfur-containing corrosion inhibitors such as 2-mercaptothanol (ME), mercaptoacetic acid (TGA), and thiourea (TU). The results show that a mixture of MBI and OIM gave a better synergistic corrosion inhibition than that of ME, TGA, and TU. OIM is protonated and coupled with MBI by hydrogen bonding to co-adsorb on the carbon steel surface. MBI adsorbs on Fe by a bidentate binding N S bridge connection, which effectively prevents the carbon steel from CO 2 corrosion.
研究领域:
材料科学
·
电化学
·
表面化学
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DOI
高影响因子
Materials
·
2020
·Vol. 13
·期 21
·4870
·
IF 3.057
Xiao Li,Jun Li,Ying Zhang,Peng Zhao,Ruyan Lei,Baige Yuan,Manman Xia
Ni(OH)2derived from an MOF template was synthesized as an electrode material for supercapacitors. The electrochemical performance of the electrode was adjusted by effectively regulating the morphology of Ni(OH)2. The evolution of electrochemical performance of the electrode with morphology of Ni(OH)2was highlighted in detail, based on which honeycomb-like Ni(OH)2was successfully synthesized, and endowed the electrode with outstanding electrochemical performance. For the three-electrode testing system, honeycomb-like Ni(OH)2exhibited a very high specific capacitance (1865 F·g−1at 1 A·g−1, 1550 F·g−1at 5 mV·s−1). Moreover, it also presented an excellent rate capability and cycling stability, due to 59.46 % of the initial value (1 A·g−1) being retained at 10 A·g−1, and 172% of initial value (first circle at 50 mV·s−1) being retained after 20,000 cycles. With respect to the assembled hybrid supercapacitor, honeycomb-like Ni(OH)2also displayed superior electrochemical performance, with a high energy density (83.9 Wh·kg−1at a power density of 374.8 W·kg−1). The outstanding electrochemical performance of Ni(OH)2should be attributed to its unique honeycomb-like structure, with a very high specific surface area, which greatly accelerates the transformation and diffusion of active ions.Keywords:honeycomb-like structure;Ni-MOF;Ni(OH)2;electrode material;supercapacitor
研究领域:
材料科学
·
电化学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2014
·Vol. 30
·期 5
·731-737
·
IF 1.119
Ma Jingwei,Kan Hong,Ma Yinghui,Men Lihui,Pi Zifeng,Liu Zhiqiang,Liu Zhongying
Ultra performance liquid chromatographic-electrospray ionization-mass spectrometry(UPLC-ESI-MS) was used to investigate the potential interaction between selected ingredients of Aconitum and fritillary. The efflux ratios of 14-benzoylmesaconine(BM), 14-benzoylaconine(BC), 14-benzoylhypaconine(BH), mesaconitine(MA), aconitine( AC) and hypaconitine(HA) was 11.16, 12.53, 11.69, 12.8, 11.03 and 6.15, respectively, and the secretion of them was inhibited by Verapamil, which means they are the substrates of permeability-glycoprotein(P-gp). The transport of Aconitum alkaloids extract through a Caco-2 cell monolayer was determined in the absence and presence of fritillary extract. And the fritillary extract increased the absorption of Aconitum alkaloids. Peimine(PE) and peiminine( PEN) in fritillary increased the absorption of pure Aconitum alkaloids. The transport of digoxin was respectively enhanced by PE and PEN, which means they are the inhibitors of P-gp. PEN showed more effective inhibition than PE at the same concentration. The in vitro data suggest that the compounds such as fritillary present in alkaloids were able to inhibit the P-gp activity and lead modifying the transport of alkaloids.
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产品关联
DOI
高影响因子
CORROSION SCIENCE
·
2021
·
IF 7.205
Hao Li,Yujie Qiang,Wenjie Zhao,Shengtao Zhang
2-Mercaptobenzimidazole (M) was successfully inbuilt in zeolitic imidazolate framework-8 (ZIF-8) on GO nanosheets and then imbedded into epoxy coating to prepare composite coating with pH-responsive and self-healing performances. Results showed that M-ZIF-8/GO possessed remarkable pH-triggered activity. The excellent self-healing behavior of M-ZIF-8/GO/EP coating was demonstrated by local electrochemical impedance spectroscopy, which was attributed to the release of corrosion inhibitors that could form a compact protective film on the metal. The anti-corrosion performance of matrix coating was also enhanced effectively because M-ZIF-8/GO could react with epoxy resin which greatly increased the compatibility of M-ZIF-8/GO and the crosslinking density of epoxy coating.
研究领域:
材料科学
·
腐蚀与防护
·
纳米技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）