ARCHIV DER PHARMAZIE
·
2019
·Vol. 353
·期 2
·1900231
·
IF 2.145
Xiujie Liu,Xin Chen,Kai Qiu,Zhihao Zhang
A series of 4-methoxy-3-arylamido- N -(substitutedphenyl)benzamides 6a – u were designed according to the splicing principle of structural design in the medicinal chemistry theory and were synthesized in five steps: nitration, acylation, ammoniation, reduction, and secondary ammoniation. The structures of the target compounds were characterized and verified by infrared, 1 H nuclear magnetic resonance (NMR), 13 C NMR, and electron spray ionization spectroscopy. Their in vitro antiplatelet aggregation activities induced by adenosine diphosphate (ADP) or arachidonic acid (AA) were assessed by Born's method. The biological evaluation revealed that all compounds exhibited certain levels of activities in both of the antiplatelet aggregation assays; compounds 6c (IC 50 = 3.84 μM) and 6f (IC 50 = 3.12 μM) displayed the strongest antiplatelet aggregation activities in the ADP-induced and AA-induced assay, separately. Moreover, compounds that had stronger activities were chosen for cell toxicity testing via the cell counting kit-8 assay. The results indicated that none of the compounds had obvious cell toxicity against L929 cells at the doses of 10 and 20 μM. It is worth pointing out that compound 6c showed the highest antiplatelet activity and the lowest cell toxicity. In general, 4-methoxy-3-arylamido- N -(substitutedphenyl)benzamides have the potential to become a kind of safer and more effective antiplatelet agents.
研究领域:
药物化学
·
药理学
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DOI
PubMed
高影响因子
Energy Storage Materials
·
2019
·
IF 0
Jian Zhang,Xudong Zhao,Yuzhu Song,Qiang Li,Yongchang Liu,Jun Chen,Xianran Xing
Na 3 V 2 (PO 4 ) 3 is one of the most promising cathodes for sodium-ion batteries (SIBs) due to its three-dimensional framework. To meet practical feasibility, the substitution of vanadium with low-cost active elements is urgent. Here the Na + superionic conductor (NASICON)-type Na 3.5 Mn 0.5 V 1.5 (PO 4 ) 3 nanoparticles homogeneously embedded in porous carbon matrix are prepared and investigated as a novel cathode for SIBs. The as-prepared Na 3.5 Mn 0.5 V 1.5 (PO 4 ) 3 /C could deliver a desirable average discharge potential of 3.43 V vs. Na + /Na with fascinating rate capability of 92.7 mA h g −1 at 60C and impressive capacity retention of 87.2% after 4000 cycles at 20C. In situ X-ray diffraction reveals that the electrochemical process undergoes highly reversible biphasic transition and single phase change with a relatively small volume change (8.21%), ensuring the high structural stability and excellent cyclic capability. The reversible evolution of Mn 2+/3+ and V 3+/4+ redox couples upon Na + extraction/insertion has been revealed by the confirmation of valence state via both ex situ X-ray absorption near-edge structure spectra and X-ray photoelectron spectroscopy. The superior performance of Na 3.5 Mn 0.5 V 1.5 (PO 4 ) 3 could be attributed to the high electronic/ionic conductivity and low sodium-ion diffusion energy barrier, which is supported by the electrochemical impedance spectroscopy and cyclic voltammetry measurements, as well as density functional theory computations.
研究领域:
材料科学
·
能源存储
·
电化学
·
电池技术
✓ 已验证
DOI
Nanoscale
·
2018
·Vol. 11
·期 3
·962-967
·
IF 7.233
Zhaohong Su,Xiaolin Xu,Yongbing Cheng,Yueming Tan,Langtao Xiao,Daili Tang,Hongmei Jiang,Xiaoli Qin,Huixian Wang
A porous graphene (PG) bionanocomposite of PG, gold nanoparticles (AuNPs) and anti-indole-3-acetic acid (anti-IAA) antibody for sensitive and label-free amperometric immunoassay of IAA was reported. A PG film was produced by a pre-reduction/electrochemical reduction process on a glassy carbon electrode (GCE) and then a homogeneous AuNPs layer electrodeposition on the PG film. The anti-IAA antibody was immobilized onto the AuNPs through electrostatic adsorption and covalent conjugation. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV), elecro-chemical impedance spectroscopy (EIS), ultraviolet visible spectroscopy (UV-vis) and differential pulse voltammetry (DPV) were used to characterize the PG film and the stepwise modification of the immunosensor. The electrochemical immunosensor exhibited a wide linear range from 2 × 10−11 to 2 × 10−8 g mL−1 with a detection limit of 0.016 ng mL−1 (S/N = 3) and showed significant linearity R2 = 0.9970. In addition, the proposed immunosensor showed acceptable selectivity and has been applied to the determination of IAA in the extract samples of several plant seeds with acceptable relative derivation (%) ranging from −5.25% to 4.24% between the immunosensor and high performance liquid chromatography. The proposed chemical pre-reduction and electro-reduction guided protocol can be extended to the preparation of many other functionalized PG nanocomposite films for wide applications.
研究领域:
生物传感
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纳米生物技术
·
分析化学
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植物生理学
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电化学
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免疫分析
✓ 已验证
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Guoqiang Zhao,Wenjing Chen,Rongrong Zhang,Rui Zhang,Bo Jiang,Ruyan Hou,Xing Huang
Cypermethrin, a common pyrethroid insecticide, raises significant ecological concerns due to its widespread use. In this study, a highly efficient cypermethrin-degrading strain, Rhodococcus sp. H-3, was isolated. The strain was capable of degrading 30 mg/L cypermethrin at the rate of 83.48 % within 7 days and it could hydrolyze cypermethrin into 3-phenoxybenzoic acid (3-PBA) and permethric acid (DCVA). A novel cypermethrin degradation gene, cymE1 , was identified in strain H-3 through genome analysis, and its highest level of amino acid sequence similarity observed was 33.50 % in the Swiss-Prot database. The functional strain, SCK6-E, which could secrete CymE1, was successfully constructed by using double-exchange strategies. Strain SCK6-E could be applied for the removal of cypermethrin residues from tea plant leaves with a removal rate of 57.83 % within 2 h. Furthermore, strain H-3 demonstrated the capability to remediate cypermethrin-contaminated soil, exhibiting a degradation rate of 65.80 % over 10 days. The results of the 16S rRNA high-throughput sequencing indicated that strain H-3 was conducive to the restoration of the diversity and richness of the bacterial community in the soil. This study provides novel strain and gene resources for researching the degradation mechanism of pyrethroids, and offers valuable insights for developing effective pesticide residue remediation strategies.
研究领域:
环境微生物学
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生物修复
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基因组学
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分子生物学
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农业科学
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产品关联
DOI
PubMed
高影响因子
MUSCLE & NERVE
·
2025
·Vol. 71
·期 3
·474-486
·
IF 3.1
Zhuajin Bi,Qing Zhang,Huajie Gao,Huizhen Ge,Jiayang Zhan,Mengge Yang,Bitao Bu
Introduction/Aims Tofacitinib, a first-generation Janus kinase (JAK) 1/3 inhibitor, is commonly used for treating ulcerative colitis and rheumatoid arthritis. However, its role in myasthenia gravis (MG) remains unclear. This study aimed to evaluate the immunomodulatory effects of tofacitinib on experimental autoimmune myasthenia gravis (EAMG) and peripheral blood mononuclear cells (PBMCs) from patients with MG. Methods Flow cytometry, enzyme-linked immunosorbent assay (ELISA), quantitative reverse transcription polymerase chain reaction (qRT-PCR), and Western blot were used to evaluate the effects of tofacitinib on T helper (Th) cell profiles, humoral immune responses, and the JAK-signal transducer and activator of transcription (STAT) pathway proteins. Results In vivo, tofacitinib significantly ameliorated EAMG severity in rats, reducing the proportions of Th1, Th17 and memory B cells, and anti-acetylcholine receptor (AChR) antibodies levels, while increasing the proportions of regulatory T (Treg) cells. In vitro, tofacitinib administration resulted in a significant decrease in the proportions of Th1 and IgG-secreting B cell, and a significant upregulation of Treg cells in mononuclear cells (MNCs) from EAMG rats, which was consistent with findings in PBMCs from MG patients. Further analysis revealed that tofacitinib inhibited CD4 + T cell differentiation into Th1 by decreasing phosphorylated STAT1 levels, while promoting Treg differentiation via increased phosphorylated STAT5 levels in MNCs from EAMG rats. Discussion Tofacitinib modulates Th cell profiles and humoral immune responses by targeting the JAK–STAT pathway, suggesting its potential as a therapeutic candidate for MG. Further clinical studies are warranted to evaluate the efficacy and safety of tofacitinib in MG patients.
研究领域:
免疫学
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药理学
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神经病学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Hongjun Zang,Yimo Feng,Jing Lou,Kai Wang,Changchun Wu,Zhipeng Liu,Xi Zhu
Ionic liquids have shown good catalytic performance and been widely used in the biomass conversion process due to their unique properties, including environmental friendship, low toxicity, and simple preparation. In this work, a series of novel piperidinium-based ionic liquids were synthesized under mild conditions via a simple protonation reaction or nucleophilic substitution reaction. Among them, 4,4′-(propane-1,3-diyl)bis(1-(carboxymethyl)piperidin-1-ium) chloride ([PDCMPi]Cl) displayed the best catalytic performance. The highest yield (42.81 %) of 3A5AF from NAG was obtained after reacting in N-methyl-pyrrolidone (NMP) in the presence of piperidinium-based ionic liquids at 180 °C for 20 min under normal atmospheric conditions. Additionally, ionic liquid [PDCMPi]Cl showed good catalytic activity in six repeated catalytic runs. Meanwhile, a possible reaction pathway was also proposed on the basis of the results of NMR spectra.
研究领域:
催化化学
·
绿色化学
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生物质能源
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有机合成
·
离子液体化学
✓ 已验证
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 408
·期 20
·5527-5535
·
IF 3.125
Lian Ziru,Li Hai-Bei,Wang Jiangtao
An innovative and effective extraction procedure based on molecularly imprinted solid-phase extraction (MISPE) was developed for the isolation of gonyautoxins 2,3 (GTX2,3) from Alexandrium minutum sample. Molecularly imprinted polymer microspheres were prepared by suspension polymerization and and were employed as sorbents for the solid-phase extraction of GTX2,3. An off-line MISPE protocol was optimized. Subsequently, the extract samples from A. minutum were analyzed. The results showed that the interference matrices in the extract were obviously cleaned up by MISPE procedures. This outcome enabled the direct extraction of GTX2,3 in A. minutum samples with extraction efficiency as high as 83 %, rather significantly, without any need for a cleanup step prior to the extraction. Furthermore, computational approach also provided direct evidences of the high selective isolation of GTX2,3 from the microalgal extracts.
研究领域:
海洋生物学
·
分析化学
✓ 已验证
DOI
高影响因子
Advanced Composites and Hybrid Materials
·
2025
·Vol. 8
·期 4
·286
·
IF 21.8
Mao Gaoqiang,Lu Jingfan,Cai Haiyan,Yu Wanjing,Tong Hui,Guo Xueyi,Zeng Leiying,Jiang Long
Increasing the operating voltage can significantly increase the energy density of LiCoO 2 , but it is accompanied by severe structural damage and interface degradation. Especially at a high voltage above 4.5 V, LiCoO 2 faces severe challenges, such as irreversible phase transition, lattice oxygen loss, and electrode–electrolyte interface evolution. Herein, a high-voltage LiCoO 2 cathode material with bulk doping and surface coating synergistic modification was designed and prepared. The doped Mg 2+ ions entered the lithium layers as pillars, stabilizing the layered framework. Meanwhile, Li 1.3 La 0.3 Ti 1.7 (PO 4 ) 3 was uniformly coated on the surface of LiCoO 2 , which is beneficial for suppressing Co 3+/2+ ion leaching and interface side reactions during cycling. Furthermore, Li 1.3 La 0.3 Ti 1.7 (PO 4 ) 3 is a fast ion conductor, which facilitates the rapid transport of lithium ions on the surface of the cathode material. Owing to the synergistic effect of Mg 2+ ion doping and Li 1.3 La 0.3 Ti 1.7 (PO 4 ) 3 coating, the orbitals of Co 3d and O 2p in LiCoO 2 crystal are changed, and the irreversible phase transition and oxygen loss are inhibited. The modified material exhibited an excellent electrochemical performance. A discharge capacity of 175.5 mAh·g −1 at 10 C rate, as well as a capacity retention of 90.14% after 200 cycles at 1 C rate, was achieved in the modified material. This work supplies a new method for developing high-voltage oxide cathodes through bulk and interfacial modification.
研究领域:
材料科学
·
电化学
·
储能技术
·
锂离子电池
·
正极材料
✓ 已验证
产品关联
DOI
高影响因子
IONICS
·
2015
·Vol. 21
·期 9
·2503-2510
·
IF 1.754
Ma Xueli,Wang Chunfeng,Ren Hao,Chen Yujuan,Zhuo Kelei
1-Hexyl-3-methylimidazolium tetraphenylborate ([C 6 mim]-TPB) was synthesized and explored as ion exchanger in carbon paste electrode (CPE) as an ion-selective electrode (ISE). The electrode was found to be optimal with the composition of 42 % graphite powder, 20 % paraffin oil, 30 % ion exchanger, 5 % MWCNTs, and 3 % nano-silica. The as-prepared electrode exhibits a Nernstian response (59.2 mV per decade) to 1-hexyl-3-methylimidazolium cation in the concentration range from 1.0 × 10 −5 to 1.0 × 10 −2 mol kg −1 . The novel as-prepared MWCNTs/nano-silica/CPE was successfully applied in the detection of [C 6 mim] + in distilled water, tap water, and river water with satisfactory results.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF BIOTECHNOLOGY
·
2015
·
IF 2.871
Yu-Cai He,Zhi-Cheng Tao,Yun Ding,Dan-Ping Zhang,Yin-Qin Wu,Yun Lu,Feng Liu,Yu-Feng Xue,Cheng Wang,Jian-He Xu
To avoid adding NAD + and effectively transform ethyl 4-chloro-3-oxobutanoate, the mixture of l -glutamine (200 mM) and d -xylose (250 mM) was added into in n -butyl acetate–water (10:90, v/v) biphasic system instead of NAD + for increasing the biocatalytic efficiency. To further improve the synthesis of optically pure ethyl ( R )-4-chloro-3-hydroxybutanoate (>99% ee ), β-cyclodextrin was also added into this reaction media, and ethyl ( R )-4-chloro-3-hydroxybutanoate (>99% ee ) could be effectively synthesized from 800 mM ethyl 4-chloro-3-oxobutanoate in the yield of 100% by whole-cells of recombinant E. coli CCZU-A13. Finally, the possible mechanism for improving the reductase activity by supplementation of l -glutamine, d -xylose and β-CD was proposed. In conclusion, this strategy has high potential for the effective biosynthesis of ethyl ( R )-4-chloro-3-hydroxybutanoate (>99% ee ).
研究领域:
生物化学
·
酶工程
·
合成生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Yuxiang Chen,Xiangyue Liao,Peng Wang,Ji Chen,Xiaoqin Zhang,Xingqiao Wu,Sean C. Smith,Dunmin Lin,Xin Tan,Qiaoji Zheng
The stable three-dimensional framework and high operating voltage of sodium superionic conductor (NASICON)-type Na 3 V 2 (PO 4 ) 3 has the potential to work with long cycle life and high-rate performance; however, it suffers from the poor intrinsic electronic conductivity and low energy density . Herein, Ga 3+ is introduced into Na 3 V 2 (PO 4 ) 3 to activate the V 4+ /V 5+ redox couple at a high potential of 4.0 V for enhancing energy density of the materials (Na 3 V 2-x Ga x (PO 4 ) 3 ). After the partial substitution of Ga 3+ for V 3+ , three redox couples (V 2+ /V 3+ , V 3+ /V 4+ and V 4+ /V 5+ ) of V are reversibly converted in the voltage range of 1.4–4.2 V, suggesting multi-electrons (>2e − ) involved in the reversible reaction, and simultaneously the electronic conductivity of the materials is effectively enhanced. As a result, the cathode with x = 0.75 exhibits excellent electrochemical properties: in the voltage range of 2.2–4.2 V, delivering an initial capacity of 105 mAh/g at 1C with a capacity retention rate of 92.3% after 400 cycles, and providing a stable reversible capacity of 88.3 mAh/g at 40C; and in the voltage range of 1.4–4.2 V, presenting the reversible capacity 152.3 mAh/g at 1C (497.6 Wh kg −1 ), and cycling stably for 1000 cycles at 20C with a capacity decay of 0.02375% per cycle. It is found that the Na 3 V 2-x Ga x (PO 4 ) 3 cathodes possess the sodium storage mechanism of single-phase and bi-phase reactions. This investigation presents a useful strategy to enhance the energy density and cycling life of NASICON-structured polyanionic phosphates by activating high-potential V 4+ /V 5+ redox couple.
研究领域:
材料科学
·
电化学
·
能源存储
✓ 已验证
DOI
高影响因子
NANOTECHNOLOGY
·
2021
·Vol. 32
·期 17
·175101
·
IF 3.874
Yanling Liu,Manman Tan,Chunjuan Fang,Xiaoyan Chen,Huan Liu,Ying Feng,Yujuan Zhang,Weiping Min
Tumor-specific targeted delivery is a major obstacle to clinical treatment of hepatocellular carcinoma (HCC). Here we have developed a novel multi-functional nanostructure GAL-GNR-siGPC-3, which consists of Galactose (GAL) as the HCC-targeting moiety, golden nanorods (GNR) as a framework to destroy tumor cells under laser irradiation, and siRNA of Glypican-3 (siGPC-3) which induce specifically gene silence of GPC-3 in HCC. Glypican-3 (GPC-3) gene is highly associated with HCC and is a new potential target for HCC therapy. On the other hand, Gal can specifically bind to the asialoglycoprotein receptor which is highly expressed on membrane of hepatoma cells. GAL and siGPC-3 can induce targeted silencing of GPC-3 gene in hepatoma cells. In vivo and in vitro results showed that GAL-GNR-siGPC-3 could significantly induce downregulation of GPC-3 gene and inhibit the progression of HCC. More notably, GAL-GNR-siGPC-3 could induce both GPC-3 gene silencing and photothermal effects, and the synergistic treatment of tumors was more effective than individual treatments. In summary, GAL-GNR-siGPC-3 achieved a synergistic outcome to the treatment of cancer, which opens up a new approach for the development of clinical therapies for HCC.
研究领域:
纳米技术
·
癌症治疗
·
分子生物学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）