JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2016
·Vol. 92
·期 4
·827-833
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IF 2.738
Jinxi Feng,Shenyu Lan,Chunyang Yao,Ya Xiong,Shuanghong Tian
BACKGROUND Electro-generation of NaOH and H 2 SO 4 with organic compound-free Na 2 SO 4 wastewater has been known for many years, but actually, lots of Na 2 SO 4 wastewater contains organic pollutants, such as black liquor, dyeing and sulphonating wastewater etc., therefore, there is a strong demand for investigations on performance and effect of the organic pollutants in electrochemically splitting Na 2 SO 4 into H 2 SO 4 and NaOH from these wastewaters. RESULTS Co-electrolysis of Na 2 SO4 (1.13 mol L −1 ) and AO7 (50 ∼ 500 mg L −1 ) was investigated in a diaphragm cell with IrO 2 -coated Ti electrodes. It was found that the concentrations of the resulting NaOH and H 2 SO 4 could remain at about 0.73 mol L −1 and 0.36 mol L −1 , respectively, although the COD removal was increased from 50.3 mg L −1 to 166.3 mg L −1 with the increase of AO7 concentration, for 120 min. CONCLUSION In a diaphragm cell with IrO 2 -coated Ti electrodes, NaOH and H 2 SO 4 could be effectively generated, and simultaneously, AO7 could also be degraded for the wastewater containing Na 2 SO 4 and AO7. Interestingly, the electro-generation of NaOH and H 2 SO 4 was not significantly affected by the occurrence and degradation of AO7 in the range 0–500 mg L −1 . The phenomenon of constant acid–base yield contributed to the independence of the main acid-generating reactions on the electro-oxidation of AO7. © 2016 Society of Chemical Industry
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JOURNAL OF LUMINESCENCE
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2024
·
IF 3.3
Shifang Wang,Mingzheng Sun,Xiaolang Fan,Bowei Liu,Chuangchuang Qiao,Shuang Jing,Xufeng Zhou,Wei Li,Yangbo Wang,Huaiyong Li
Broad band near-infrared emitting phosphors activated with Cr 3+ ions have great potential in a variety of applications. The luminescence efficiency and thermal stability are two critical parameters that are highly concerned in practical applications. In this work, a series of Cr 3+ ion-activated Ca 9 Ga(PO 4 ) x (VO 4 ) 7-x were synthesized. The photoluminescence (PL) properties of Cr 3+ were studied as functions of coordination environment, Cr 3+ concentration and temperature. The coordination environment of Cr 3+ ions is modified by gradually substitution of P by V without change the crystal structure of the host lattice. A continuous solid solution is formed, and the cell parameters and volume increase monotonically with V content. Great improvement in the intensity is observed for Ca 9 Ga(PO 4 ) 3.5 (VO 4 ) 3.5 , which increases by 26 and 2.4 times than the parents compositions Ca 9 Ga(PO 4 ) 7 and Ca 9 Ga(VO 4 ) 7 , respectively. Decay dynamic analysis suggests that both coordination environment modification and reduction of quenching center responsible for the great enhancement. An optimized concentration of 3.0 % is revealed and the intensity at 433 K maintains 81.1 % of the intensity at 293 K, respectively.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Huiyu He,Rongbin Nie,Nan Dong
Biogenic amines (BAs) are the vital indicators of food freshness, and monitoring the change of BAs content is quite essential for food safety. Herein, we developed a matrix solid phase dispersion (MSPD) extraction technology based on cucurbit[7]uril (Q[7]) to extract BAs from pork and shrimp. Then, the extracted BAs were analyzed by capillary electrophoresis (CE) with low sample consumption and high analytical efficiency. The factors affecting MSPD extraction were optimized by Plackett-Burman and the Box-Behnken design (BBD). The limits of detection (LODs) were ranging from 0.003 to 0.019 mg kg −1 , the recoveries of spiked samples were 80.15 %–110.3 %. The satisfactory accuracy and precision were attributed to the host–guest interaction between Q[7] and BAs. Furthermore, the freshness of pork and shrimp was evaluated by simultaneous determination of four BAs. For its wide linear range, high sensitivity, and cost-effectiveness, the proposed Q[7] mediated MSPD-CE-UV provides meaningful potential prospects in the rapid monitor of food freshness and safety.
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Biomaterials Science
·
2024
·Vol. 12
·期 20
·5266-5273
·
IF 5.8
Tao Hu,Ji-Na Hao,Yongsheng Li
Rapid and accurate detection of 7-methylguanine (m7Gua), a biomarker reflecting the degree of DNA methylation that occurs before or in the early stage of cancer, is of particular significance but remains a great challenge. Herein, a luminescent lanthanide-based covalent organic framework (Ln-COF) probe, namely DPA/Eu@ETTA-DHTA, is designed for the first time for the identification of m7Gua by assembling pyridine-2,6-dicarboxylic acid (DPA) as both an energy donor and a recognition molecule and Eu3+ ions as signal reporters into a stable COF matrix with high porosity and available binding sites. Significantly, the characteristic luminescence of Eu3+ ions can be turned on by the grafted DPA in the COF probe and effectively quenched by the addition of m7Gua via a competitive absorption process, thus achieving the sensing of m7Gua. Such a Ln-COF-based fluorescent platform presents high selectivity and a rapid response (<1 min) to m7Gua with a low detection limit (μM level) even in the presence of the main coexisting species in urine, allowing it to serve as a potentially practical probe for point-of-care monitoring of the level of m7Gua in human urine specimens. This study provides a convenient, time-saving, and economical approach for visual detection of m7Gua, and opens up new perspectives for the design of a luminescent COF-based probe for DNA methylation evaluation in diagnostics.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Wenqing Li,Shimin Zhang,Songchao Shi,Xiaoying Cheng,Chongwei An,Jingyu Wang,Baoyun Ye
Realizing the balance between high energy density and multifunctionality of energetic materials is a hot spot in current research, and its study is of great significance for the application of energetic materials. Regulating microstructure is widely recognized as an effective strategy for solving such problems. In this paper, four different microstructures of Hexanitrohexaazaisowurtzitane(CL-20)/1,1-diamino-2,2-dinitroethylene(FOX-7) composite microspheres, namely, hollow, porous irregular microspheres, porous microspheres, and layered porous microspheres, were prepared by microchannel recrystallization technology. The effects of crystal precipitation rate, binder mechanical properties, and purity of water phase on the microstructure of composite microspheres were systematically investigated. The four prepared samples were spherical particles with regular morphology and uniform particle size. The angle of repose and mechanical sensitivity tests showed that the CL-20/FOX-7 composite microspheres had good dispersibility and mechanical sensitivity. Ignition experiments show that hollow microspheres have the best energy release efficiency, while porous microspheres have higher energy density and more stable energy release efficiency. The results show that the combustion performance and safety of the composite microspheres can be effectively enhanced by adjusting the microstructure. Microchannel recrystallization technology can realize the controllable preparation of the microstructure of composite microspheres, which provides a reference for the design and preparation of other composite microspheres.
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ENZYME AND MICROBIAL TECHNOLOGY
·
2024
·
IF 3.4
Shuqi Xing,Wei Xie,Guangli Hu,Chaocheng Luo,Hong Zhu,Laping He,Cuiqin Li,Xiao Wang,Xuefeng Zeng
GDSL-type esterases are promising biocatalysts for the food and pharmaceutical industries. Here, a GDSL-type esterase from Aspergillus niger CCTCC No. M2012538 (INANE1) was expressed and purified in Pichia pastoris GS115, and its catalytic performances were evaluated, including the synthesis of cinnamyl acetate and deacetyl-7-aminocephalosporanic acid (D-7-ACA). In addition, molecular docking and molecular dynamics simulations analyzed INANE1's substrate specificity. The substrate specificity profile indicated the recombinant esterase (rINANE1) was an acetylesterase with high specificity for p -nitrophenyl acetate ( p -NPA). The rINANE1 exhibited maximum activity at pH 8.0 and 35 °C, where K m and V max were calculated as 0.13±0.03 mM and 22.56 ± 0.32 μmoL/min/mg, respectively. The yield of cinnamyl acetate of about 85 % was achieved in 24 h. The conversion rate of 7-aminocephalosporanic acid (7-ACA) could reach 92.71 ± 1.78 % at 25 °C and 2.5 h. Moreover, the INANE1 structure model, molecular docking, and molecular dynamics simulation demonstrated that the pocket of the catalytic triad Ser34, Asn267, and His270 could only accommodate p -NPA. INANE1 may be the first fungi esterase with cinnamyl acetate synthetic activity and 7-ACA hydrolysis activity. Therefore, INANE1 would be a promising enzyme with industrial values.
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DRUG METABOLISM AND DISPOSITION
·
2024
·Vol. 52
·期 11
·1323-1331
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IF 4.4
Han Liu,Lanjing Li,Ting Liang,Ru Huan,Bruno Hagenbuch,Chunshan Gui
Human organic anion transporting polypeptide (OATP) 1B1 and 1B3 are two highly homologous liver-specific uptake transporters. However, 2’,7’-dichlorofluorescein (DCF) is preferably transported by OATP1B1. In the present study, the molecular mechanisms for the selective transport of DCF by OATP1B1 were investigated by constructing and characterizing an array of OATP1B1/1B3 chimeras and site-directed mutagenesis. Our results show that transmembrane domain (TM) 10 is crucial for the surface expression and function of OATP1B1, in which Q541 and L545 play the most important roles in DCF transport. Replacement of TM10 in OATP1B1 with its OATP1B3 counterpart led to OATP1B1’s complete intracellular retention. Q541 and L545 may interact with DCF directly via hydrogen bonding and hydrophobic interactions. The decrease of DCF uptake by Q541A and L545S was due to their reduced binding affinity for DCF as compared with OATP1B1. In addition, Q541 and L545 are also crucial for the transport of estradiol-17β-glucuronide (E17βG) but not for the transport of estrone-3-sulfate (E3S), indicating different interaction modes between DCF/E17βG and E3S in OATP1B1. Taken together, Q541 and L545 in TM10 are critical for OATP1B1-mediated DCF uptake, but their effect is substrate-dependent.
研究领域:
药理学
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分子生物学
·
细胞生物学
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2015
·
IF 0.476
S.E. Eltayeb,Zhigui Su,Yanyu Xiao,Qineng Ping
Diverse lines of research show that the cellular response to artemether (ART) is multi-factorial in nature. The cytotoxicity of ART is specific to cancer cells because most of them over expressed; transferrin receptors and have high level of intracellular iron and ART is mainly toxic after; interaction with iron ion. Our aim is to investigate the effect of some formulation characteristics on; the cytotoxicity of ART in C6 and MCF-7 cells lines. In this study, the cytotoxicity of ART-loaded; anionic, cationic or neutral (transferrin modified) lipid nanospheres was studied by MTT and; apoptosis tests. Characterizations of apoptosis were done. The effect on the mitochondria and the; nucleus was qualitatively characterized after using different types of cell tracker dyes. The cellular; uptake, accumulation and distribution of the formulations were characterized after loading; different hydrophobic fluorescence probes instead of ART. The relation between the; accumulated amount of ART and its cytotoxicity was defined. Our study shows that ART can be highly toxic to the tumor cells if accumulated in a large amount in the cell. Lipid nanosphere containing tween80 and transferrin can highly accumulate ART in brain cells and can be; formulated as a promising potent, safe and inexpensive drug carriers for brain tumors.
研究领域:
药理学
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肿瘤学
·
纳米技术
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JOURNAL OF CONTROLLED RELEASE
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2026
·
IF 12.4
Zhongshi Qiao,Danping Liu,Rui Huang,Yingxin Chen,Hui Li,Meiyun Hao,Xinke Zhang,Xiaoqing Li,Zhiyue Zhang,Xuezhen Ma
A major bottleneck in radio-immunotherapy is the inability to precisely couple the localized stimulus of radiotherapy (RT) with the systemic action of immunotherapeutics. To bridge this gap, we developed a chemically engineered RT-ignited nanoplatform that productively harnesses the biochemical consequences of RT to precisely activate immunotherapy locally, thereby amplifying its benefits while minimizing systemic exposure. The platform co-delivers a hydrogen sulfide (H₂S)-responsive prodrug of toll-like Toll-like receptor 7/8 (TLR7/8) agonist imiquimod (IMQ) using a reactive oxygen species (ROS)-sensitive, targeted nanocarrier. RT ignites a biochemical sequence: ROS bursts disassemble the carrier, and the subsequent H₂S upregulation cleaves the prodrug, ensuring tumor-localized drug release. This RT-primed, sequentially activated therapy profoundly enhances antitumor immunity by inducing immunogenic cell death (ICD), activating antigen-presenting cells (APCs), and overcoming systemic immunosuppression, culminating in durable tumor regression and immunological memory. This study highlights a chemistry-driven approach to spatially and temporally confine immune activation to the tumor site, effectively broadening the therapeutic window and transforming RT-induced biochemical changes into a precise trigger for systemic immunity. Download: Download high-res image (237KB) Download: Download full-size image
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Toxics
·
2026
·Vol. 14
·期 3
·222
·
IF 4.9
Xiao Kang,Wenting Song,Xueyan Li,Yuyan Yang,Xinke Wu
Breast cancer is the most common malignant tumor among women worldwide, and its occurrence is closely associated with long-term exposure to environmental pollutants. Polycyclic aromatic hydrocarbons (PAHs) are a class of persistent organic pollutants widely present in the living environment. Epidemiological studies indicate that exposure to PAHs increases the risk of breast cancer. PAH derivatives exhibit stronger toxicity or endocrine-disrupting activity than their parent compounds; however, research on their specific effects and mechanisms in breast cancer cells remains limited. For this purpose, this study selected 3-Hydroxybenz[a]anthracene, a PAH derivative with potential estrogenic activity, as the target compound. Using the estrogen receptor-positive breast cancer cell line MCF-7 as the model, we performed EdU staining, colony formation assays, scratch healing assays, Transwell invasion assays, and apoptosis assays and preliminarily examined changes in relevant signaling proteins via Western blot. Results indicate that 3-Hydroxybenz[a]anthracene promotes proliferation and migration in MCF-7 cells while inhibiting apoptosis under certain conditions, but it has no effect on cell invasion. Mechanistically, it upregulates key proteins including AKT, c-Myc, E-Cadherin, Vimentin, MMP2, MMP9 and Bcl-2 while downregulating p-AKT expression. This study confirms through in vitro experiments that 3-Hydroxybenz[a]anthracene exhibits estrogen-like effects and modulates malignant behavior in breast cancer cells by regulating relevant signaling pathways. These findings provide experimental evidence for further evaluating the potential role of this environmental contaminant in breast cancer initiation and progression.
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Food Bioscience
·
2026
·
IF 6.2
Wei Tao,Xinyu Shan,Said Nawab,Zhangchenyu Ni,Elvis Kwame Adinkra,Mingyu Guo,Yifeng Jiang,Yu Chen,Chuanchao Wu,Yan Liu
Menaquinone-7 (MK-7), a subtype of Vitamin K 2 , is in high demand within the healthcare sector due to its health benefits. This study proposes a two-stage fermentation strategy combining submerged fermentation with leakage fermentation to enhance MK-7 production in a Bacillus subtilis mutant strain and preliminarily reveal its potential metabolic mechanisms. The Results showed that this strategy achieved a total MK-7 yield of 163.56 mg/L, representing an 85.48% increase over conventional biofilm fermentation, among which extracellular MK-7 production was significantly increased by 269.65%. Results from scanning electron microscopy, fluorescence microscopy, and flow cytometry confirmed that the enhanced MK-7 production was closely associated with increased membrane permeability induced by Brij-58. Furthermore, integrating gas chromatography–ion mobility spectrometry (GC-IMS) and untargeted metabolomics analyses revealed that the highly active central carbon metabolism in the first stage promoted cell growth and MK-7 precursor accumulation, while the possible diversion of carbon flux to secondary metabolic pathways in the second stage facilitated efficient MK-7 biosynthesis. Meanwhile, it was found that the increase in membrane permeability might be attributed to oxidative stress induced by Brij-58, thereby facilitating the efflux of intracellular metabolites and effectively alleviating excessive intracellular MK-7 accumulation. These findings could serve as a potential industrial reference for future process optimization and scale-up validation of MK-7 fermentation, while also offering useful insights for addressing yield bottlenecks in other microbial metabolites.
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RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
·
2025
·Vol. 51
·期 6
·2582-2592
·
IF 1.7
Wang Yong,Liu Li-Na,Wei Shang-Hua,Wang Wei,Liu Cong-Jun,Gao Song,Yu Shu-Ping,Yao Ning,Wang Yu-Fei
Objective: Dehydroabietic acid (DHAA) is an important natural triterpene resin acid with a wide range of pharmacological activities, including antibacterial, antifungal, antiviral, and anti-inflammatory effects, particularly in antitumor activity. The aim of this study was to synthesize a series of novel dehydroabietic acid 1,2,3-triazole derivatives and evaluate their antitumor activity. Methods: Click chemistry was employed to conjugate dehydroabietic acid (DHAA) with 1,2,3-triazole, yielding 12 DHAA derivatives containing 1,2,3-triazole with yields ranging from 64.5 to 90%. The structures of the target compounds were confirmed by 1 H, 13 C NMR, and HR-MS (ESI). The initial antiproliferative activity of these compounds was assessed in vitro using two tumor cell lines (human cervical cancer cells, HeLa, and human breast carcinoma cells, MCF-7), with cisplatin used as a reference. Results and Discussion: The results indicated that three compounds, VII , VIII , and IX , exhibited significant antiproliferative activity against the tumor cell lines. Among them, compound VIII (4-(4-fluorophenyl)-1-(((1 R ,4a S )-7-isopropyl-1,4a-dimethyl-1,2,3,4,4a,9,10,10a-octahydrophenanthren-1-yl)methyl)-1 H -1,2,3-triazole) demonstrated particularly remarkable activity, with an IC 50 value of 22.1 μM against HeLa cells and 23.2 μM against MCF-7 cells. Conclusions: This study provides useful strategies for the design and synthesis of novel antitumor agents. The synthesized compounds exhibit some inhibitory effects on tumor cell growth and proliferation while causing minimal damage to normal cells, warranting further investigation.
研究领域:
药物化学
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肿瘤学
·
有机合成
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药理学
·
天然产物研究
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危险品化学品经营许可证（带存储）