JOURNAL OF APPLIED ELECTROCHEMISTRY
·
2024
·Vol. 54
·期 8
·1807-1817
·
IF 2.4
Shang Ling-Jun,Yu Shuai-Qun,Shang Xin-Wei,Wei Xiang-Yu,Wang Hui-Ying,Jiang Wen-Shuai,Ren Qiong-Qiong
Regular monitoring of blood glucose levels is crucial in diabetes management. Commonly used procedures may be invasive and can lead to cross-infection and inflammation. Therefore, a safe, non-invasive blood glucose-monitoring technology is needed. Herein, a 3D reduced graphene oxide (rGO) electrode was modified with MXene (Ti 3 C 2 ) and gold nanoparticles (AuNPs) to construct a non-invasive, saliva glucose detection electrode. The Au/rGO-Ti 3 C 2 electrode exhibited good detection performance for glucose concentrations in the range of 10 µM–21 mM at a working potential of 0.6 V, with a detection limit of 3.1 µM glucose, and a sensitivity of 355 µA mM −1 cm −2 . The Au/rGO-Ti 3 C 2 electrode showed almost no response to the addition of interfering substances, and no effect was observed upon subsequent glucose detection. The sensor could detect glucose in artificial saliva and saliva supernatant. The modified electrode showed a significant response to low glucose concentrations, even at 10 µM. These results indicate the suitability of the Au/rGO-Ti 3 C 2 electrode for saliva glucose detection. The electrode developed in this study has great potential in improving the efficiency of diabetes monitoring through pain-free, non-invasive glucose detection in saliva. Graphical abstract
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2022
·
IF 5.999
Laibao Zheng,Wenjia Dong,Chaochuan Zheng,Yunqiu Shen,Ruolan Zhou,Zhenxing Wei,Zhixuan Chen,Yongliang Lou
Rapid and portable detection of foodborne pathogens is of great significance for food safety and public health. The colorimetric methods based on naked-eye have been demonstrated to be a suitable qualitative method for point-of-care testing (POCT). However, analytical instruments like a microplate reader must be needed for the quantitative assay. To overcome its limitation, we herein report a novel photothermal method for foodborne pathogens based on the photothermal effect of aggregated mercaptophenylboronic acid-functionalized AuNPs (MPBA-AuNPs) induced by MPBA to translate the colorimetric detection into a simple temperature measurement using thermometers as the readout. The aggregated AuNPs show higher photothermal conversion efficiency than well-separated AuNPs under 660 nm laser irradiation. In the presence of bacteria, MPBA-AuNPs will attach to the surface of bacteria and keep separated from aggregation induced by excess MPBA, resulting in a lower temperature increase under 660 nm laser irradiation. Using E. coli O157:H7 as a model target, a good linear relationship is observed between temperature increase and bacteria concentration from 1.00 × 10 5 -1.00 × 10 9 cfu mL −1 (R 2 = 0.9877) with a detection limit of 1.97 × 10 4 cfu mL −1 , which is three orders of magnitude lower than of the MPBA-AuNPs-based colorimetric assays . The proposed photothermal method provided a universal platform for rapid and portable detection of broad-spectrum bacteria strains in real samples.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-11
·
IF 5.3
Wei Yuxin,Fu Yang,Li Chuanhai,Chen Siyu,Xie Linzhi,Chen Mei
A electrochemical biosensor was designed utilizing a CRISPR Cas9n-driven DNA walker combined with gold-nanosphere-like covalent organic frameworks (COFs-AuNPs) to detect breast cancer markers (PIK3CA E545K ctDNA). The DNA walker probe is activated only in the presence of circulating tumor deoxyribonucleic acid (ctDNA), binding to a support probe to form a double strand that is then specifically cleaved by the Cas9n/sgRNA complex. This cleavage produces numerous DNA fragments for signal amplification. The COF-AuNPs as electrode materials facilitate electronic transfer and provide additional active sites for the immobilization of nucleic acid probes. This setup achieves a detection limit of 1.76 aM, demonstrating high sensitivity. Additionally, Cas9n improves the specificity of the sensor, accurately distinguishing a pair of base-mismatched sequences, and reducing the occurrence of false positives. Overall, the sensor exhibits excellent selectivity, reproducibility, and potential for early diagnosis of breast cancer. Graphical Abstract
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高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Mingzhu Liu,Xueyan He,Bo Peng,Jin Wu,Zunquan Zhao,Jialei Bai,Jingran Sun,Yanjun Fang
Highly mimetic enzymatic Mn₃O₄/gold nanoparticle (AuNP) composites were prepared, which catalyze the oxidation of tert-butylhydroquinone (TBHQ) to red 2-tert-butyl-1,4-benzoquinone (TBBQ) for TBHQ detection. Absorbance at 487 nm (A₄₈₇) correlates linearly with TBHQ concentrations in the range of 0.52–50 μg/mL, with a limit of detection (LOD) of 0.063 μg/mL, and a limit of quantification (LOQ) of 0.21 μg/mL. TBBQ quenches the fluorescence of copper/iodine-doped carbon dots (Cu,I-CDs) at 500 nm via the inner filter effect (IFE), forming an “on-off” fluorescence probe for TBHQ. TBHQ concentration correlates linearly with the fluorescence intensity difference (F₀ − F) in the range of 0.31–54 μg/mL, with an LOD of 0.054 μg/mL and an LOQ of 0.18 μg/mL. This study uses a dual-mode detection approach to determine TBHQ in various food samples, with recovery rates ranging from 82.5% to 103.0%. The method is sensitive, accurate, cost-effective, and suitable for the rapidly analyzing of TBHQ in food.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Yan Wang,Kai Li,Zhen Xing-Wang,Shi Lei-Bao,Li Rui-Sun,Shuai Zhang,Jia Ying-Xin,Siew Young Quek,Na Zhang
Starch is an important raw material in the food industry, and the ratios of amylose to amylopectin within it is closely related to the quality of starch products. Therefore, the development of rapid detection methods for the amylose/amylopectin ratios in starch is a practical demand to meet industrial development. Consequently, the development of a rapid, sensitive, and portable detection method is of great significance. In this study, an electrochemical detection method for the amylose-to-amylopectin ratios was established by utilizing alcohol dehydrogenase (ADH) and pullulanase to fabricate an ADH@pullulanase-AuNPs biosensor. Specifically, protein-metal nanoflowers were synthesized by regulating the interactions of Cu 2+ and PO 4 3− with the enzymes to achieve the spatially localized immobilization of ADH and pullulanase; meanwhile, AuNPs were modified to enhance electrical conductivity. Additionally, the applicability of this method to rice products was investigated. The experimental results showed that the ADH@pullulanase cluster exhibited a porous flower-like structure, good enzymatic activity, and excellent electrochemical performance. Under optimal conditions, this method exhibited a wide linear range (1 × 10 −6 –1 × 10 −4 mol/L) for the amylose/amylopectin ratios, a low limit of detection (0.5 × 10 −6 mol/L), and a short detection time (< 10 min). When applied to the detection of rice products, the average relative deviation between it and the iodine colorimetric method was 6.9%. The successful development of this method provides a technical reference and theoretical basis for the on-site rapid and sensitive detection of the amylose/amylopectin ratios.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.1
Zhenyu Wang,Tong Qi,Hebin Sun,Ningran Wang,Wenbin Zhang,Jianlong Zhao,Liancheng Zhao,Lijuan Liang
The creation of dependable analytical platforms capable of detecting multiple heavy metal ions (HMIs) concurrently continues to be a significant challenge in environmental monitoring. This study introduces an innovative composite sensing material composed of gold nanoparticles (AuNPs) modified UiO-66-NH 2 metal organic frameworks (MOFs) and carbon nanotubes (CNTs) for simultaneous detection of multiple HMIs. UiO-66-NH 2 , with its extensive specific surface area and three-dimensional porous framework, facilitates effective ion diffusion and target preconcentration. The interconnected CNTs network significantly enhances electron transfer kinetics and expands the electroactive surface area, as confirmed by cyclic voltammetry (CV) analyses. AuNPs can effectively amplify electrochemical reactions and enhance the sensitivity of electrochemical detection. The thoughtful design of this sensing material amalgamates the unique benefits of its constituents. This sensing material attains remarkably low limits of detection (LOD), which is 0.021 μg·L −1 for Cd 2+ and 0.008 μg·L −1 for Pb 2+ , showcasing outstanding resistance to interference in complex environments. The potential applicationsapplications of MOF-based composites in the electrochemical sensors of HMIs are expanded by this methodology.
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MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Linzhi Li,Xing Liu,Benchao Su,Huiyin Zhang,Rui Li,Zilong Liu,Qi Chen,Tianzeng Huang,Hongmei Cao
A novel electrochemical immunoassay based on nanobody heptamer for ultrasensitive detection of alpha fetoprotein (AFP) is proposed. First, AFP heptamer fusion protein (A1-C4bp α) is prepared via fusing the AFP-specific nanobody (A1) with the C-terminal fragment of C4-binding protein (C4bp α). Then A1-C4bp α heptamer with higher affinity is used as the molecule recognizing probe to capture numerous the AFP, increasing the sensitivity of the immunosensor. Moreover, AuNPs decorated zeolitic imidazolate framework ( [email protected] ) with large surface area, and abundant binding sites serves an ideal nanocarrier to increase heptamer loading. Multiwalled carbon nanotube (MWCNTs) with excellent electrical conductivity was immobilized on glassy carbon electrode (GCE) to enhance electrochemical signals. The coupling between the [email protected] @MWCNTs and heptamer technologies constructed an ultrasensitive and specific immunosensor for AFP detection in the range of 0.1 to 10 5 pg mL −1 with a lower detection limit of 0.033 pg mL −1 . The as-prepared electrochemical sensing exhibited excellent performance and could be employed to determine AFP with satisfactory results in actual serum samples, suggesting that the proposed strategy for nanobody multimerization provides an alternative scheme for enhancing sensitivity in important biomarkers detection.
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ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Yanping Zhao,Xu Geng,Xiaoling Zhou,Li Xu,Shuai Li,Zhengqiang Li,Yi Guo,Chen Li
Pyruvate participates in diverse metabolic pathways in the body and is normally present in human blood at 40–120 μM, with concentrations outside this range associated with various diseases. Therefore, accurate and stable blood pyruvate level tests are necessary for effective disease detection. However, traditional analytical techniques require complicated instrumentation and are time consuming and expensive, prompting researchers to develop improved methods based on biosensors and bioassays. Here, we designed a highly stable bioelectrochemical pyruvate sensor affixed to a glassy carbon electrode (GCE). To maximize biosensor stability, 0.1 U of lactate dehydrogenase was affixed to the GCE using a sol-gel process, resulting in generation of Gel/LDH/GCE. Next, 2.0 mg/mL AuNPs-rGO was added to enhance current signal strength, resulting in generation of the bioelectrochemical sensor Gel/AuNPs-rGO/LDH/GCE. AuNPs-rGO synthesized in advance was verified as correct using transmission electron microscopy and UV–Vis, Fourier-transform infrared and X-ray photoelectron spectroscopy. Pyruvate detection conducted via differential pulse voltammetry in phosphate buffer (pH 7.4, 100 mM) at 37 °C for 1–4500 μM pyruvate provided detection sensitivity as high as 254.54 μA/mM/cm 2 . The reproducibility, regenerability and storage stability were analyzed with the relative standard deviation of 5 bioeletrochemical sensors detection was 4.60% and biosensor accuracy after 9 cycles was 92%, with accuracy remaining at 86% after 7 days. In the presence of D-glucose, citric acid , dopamine, uric acid and ascorbic acid , the Gel/AuNPs-rGO/LDH/GCE sensor exhibited excellent stability, high anti-interference ability and better performance than conventional spectroscopic methods for detection of pyruvate in artificial serum.
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BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Wenjing Wang,Huabiao Tang,Yuan Wu,Yinli Zhang,Zhaohui Li
A non-enzymatic and highly electrocatalytic H 2 O 2 biosensor was proposed by using a novel electrode composed of hemin-capped biomineralized gold nanoparticles ( [email protected] ), reduced graphene oxide (rGO) and chitosan (CS). Owing to the excellent electrocatalytic activity of [email protected] and great conductivity of rGO, this developed biosensor is capable of ultrasensitive measuring H 2 O 2 in real-time. In addition, the introduction of CS resulted in a film on the electrode, which can enhance the stability by protecting the biosensor from external disturbances . Taking advantages of the peroxidase-like activities of the nanohybrids , the developed electrode exhibited outstanding electrochemical performance toward H 2 O 2 with high selectivity , fast response, superior sensitivity and good stability. More importantly, the lower determination limit of 9.3 nM and wider linear ranges of 5 orders of magnitude enable this biosensor to detect H 2 O 2 releasing from living Hela cells accurately. This work provides tremendous potential for real-time monitoring the secretion of H 2 O 2 in living cells.
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TALANTA
·
2017
·
IF 4.162
Xiaofang Ma,Yali Wu,Samrat Devaramani,Caizhong Zhang,Qixia Niu,Mahgoub Ibrahim Shinger,Wenqi Li,Duoliang Shan,Xiaoquan Lu
The GO-COOH/AuNPs/ZnAPTPP nanocomposites were constructed using zinc monoamino porphyrin (ZnAPTPP) through π–π conjugation with carboxylated graphene oxide (GO-COOH) loaded with Au nanoparticles (AuNPs). Prepared materials were characterized by 1 H NMR spectra, UV–vis absorption spectroscopy and electrochemical impedance spectroscopy . ITO electrode surface was modified with the prepared nanocomposites showed a good photocurrent response when the bias potential, −0.1 V was applied. Nanocomposites modified ITO electrode exhibited good photo-response to the 4-nitrophenol (4-NP). ZnAPTPP were excited from HOMO to LUMO under light irradiation , the photoexcited electrons injected into the conduction band of GO-COOH, and then transferred to AuNPs further to the ITO. The presence of GO-COOH and AuNPs improved the separation of photogenerated charges due to their synergetic effect and excellent conductivity. Externally added 4-NP scavenges the photogenerated holes i.e. it acts as a sacrificial electron donor thereby it enhances the photocurrent of the system. Based on this interaction, a novel method for photoelectrochemical detection of 4-NP was developed with a linear range from 0.1 to 15 nmol/L (r = 0.996) and detection limit of 0.04 nmol/L (S/N = 3). Proposed method is simple and sensitive and this was successfully applied for the quantification 4-NP in river water sample matrices.
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BIOSENSORS & BIOELECTRONICS
·
2012
·
IF 5.602
Huanshun Yin,Yunlei Zhou,Haixia Zhang,Xiaomeng Meng,Shiyun Ai
MicroRNAs (miRNAs), a kind of small, endogenous, noncoding RNAs (∼22 nucleotides), might play a crucial role in early cancer diagnose due to its abnormal expression in many solid tumors. As a result, label-free and PCR-amplification-free assay for miRNAs is of great significance. In this work, a highly sensitive biosensor for sequence specific miRNA-21 detection without miRNA-21 labeling and enrichment was constructed based on the substrate electrode of dendritic gold nanostructure (DenAu) and graphene nanosheets modified glassy carbon electrode. Sulfydryl functionalized locked nucleic acid (LNA) integrated hairpin molecule beacon (MB) probe was used as miRNA-21 capture probe. After hybridized with miRNA-21 and reported DNA loading in gold nanoparticles (AuNPs) and biotin multi-functionalized bio bar codes, streptavidin–HRP was brought to the electrode through the specific interaction with biotin to catalyze the chemical oxidation of hydroquinone by H 2 O 2 to form benzoquinone. The electrochemical reduction signal of benzoquinone was utilized to monitor the miRNA-21 hybridization event. The effect of experimental variables on the amperometric response was investigated and optimized. Based on the specific confirmation of probe and signal amplification, the biosensor showed excellent selectivity and high sensitivity with low detection limit of 0.06 pM. Successful attempts are made in miRNA-21 expression analysis of human hepatocarcinoma BEL-7402 cells and normal human hepatic L02 cells.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 10
·1-13
·
IF 5.3
Guo Liang,Zhou Shijin,Xue Jinyan,Liu Zenghui,Xu Shuqing,He Zhangxu,Yang Huaixia
A sandwich electrochemical sensor was fabricated based on multi-walled carbon nanotubes/ordered mesoporous carbon/AuNP (MWCNTs/CMK-3/AuNP) nanocomposites and porous core–shell nanoparticles Au@PdNPs to achieve rapid and sensitive detection of AFB 1 in complex matrices. MWCNTs/CMK-3/AuNP nanocomposite, which was prepared by self-assembly method, served as a substrate material to increase the aptamer loading and improve the conductivity and electrocatalytic activity of the electrode for the first signal amplification. Then, Au@PdNPs, which were synthesized by one-pot aqueous phase method, were applied as nanocarriers loaded with plenty of capture probe antibody (Ab) and signal molecule toluidine blue (Tb) to form the Au@PdNPs-Ab-Tb bioconjugates for secondary signal amplification. The sensing system could still significantly improve the signal output intensity even in the presence of ultra-low concentration target compound due to the dual signal amplification of MWCNTs/CMK-3/AuNP nanocomposites and Au@PdNPs-Ab-Tb. The method exhibited high selectivity, low detection limit (9.13 fg/mL), and strong stability to differentiate AFB 1 from other mycotoxins. Furthermore, the sensor has been successfully applied to the quantitative determination of AFB 1 in corn, malt, and six herbs, which has potential applications in food safety, quality control, and environmental monitoring. Graphical Abstract
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高影响因子
危险品化学品经营许可证（带存储）