ACS Applied Materials & Interfaces
·
2014
·
IF 5.9
Guilong Zhang,Minglei Yang,Dongqing Cai,Kang Zheng,Xin Zhang,Lifang Wu,Zhengyan Wu
An efficient enzyme-responsive controlled release carrier system was successfully fabricated using single-stranded DNA encapsulated functional mesoporous silica nanoparticles. Mesoporous silica nanoparticles were initially fabricated through hydrolysis of tetraethyl orthosilicate (TEOS) in cetyltrimethylammonium chloride (CTAC) solution, and the surface of nanoparticles could be encapsulated with single-stranded DNA. This nanomaterial has excellent bioactivity and its hydrolysate cannot cause damage to normal cell, thus the biocompatibility of the nanomaterial is improved. In addition, this nanomaterial showed an excellent drug release performance when loaded with drugs, which would be helpful to increase the treatment efficiency and decrease side effects of drugs.
研究领域:
材料科学
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生物医学工程
·
纳米技术
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DOI
PubMed
高影响因子
Nanoscience and Nanotechnology Letters
·
2019
·Vol. 11
·期 8
·1139-1144
·
IF 0
Gu Mengqiao,Liu Jiali,Li Delei,Wang Min,Chi Kuanneng,Zhang Xi,Deng Yan,Ma Yuchan,Hu Rong,Yang Yunhui
In this paper, a signal-off aptasensor was constructed based on the specificity of OTA and OTA aptamer (DNA2), it can detect the small molecule ochratoxin A (OTA). DNA1 chain with mercapto modified at 3′-end was firstly immobilized on gold electrode, which cross-fertilize with
DNA 2 to form double strands. 30 thymine T modified at the end of DNA2 were used as templates to synthesize Cu NCs which is peroxidase mimics and can catalyze hydrogen peroxide. Presence of OTA, it specifically binds to DNA2, which made DNA2 dissociated, and so did Cu NCs. The more OTA in
solution, the more the aptamers and Cu NCs dissociated from the gold electrode, the less copper nanoclusters remained on the electrode, and the smaller the current response value of the electrode measured by chronoamperometry. Thus, the quantitative determination of OTA can be achieved. This
aptasensor provides a simple and fast technique for OTA detection in red wine samples.
研究领域:
分析化学
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食品安全
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纳米技术
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生物传感器
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DOI
Cell Death & Disease
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2025
·Vol. 16
·期 1
·1-14
·
IF 9.6
Liu Min,Li Yuanyuan,Deng Zhilin,Zhang Ke,Huang Shuying,Xia Jiamin,Feng Yi,Liang Yundan,Sun Chengfu,Liu Xindong,Li Shurong,Su Bingyin,dong Yong,Huang Sizhou
Mutation in genes involved in DNA replication continuously disrupt DNA replication and give rise to genomic instability, a critical driver of oncogenesis. To prevent leukemia, immature T lymphocytes with genomic instability often undergo rapid cell death during development. However, the mechanism by which immature T lymphocytes undergo rapid cell death upon genomic instability has been enigmatic. Here we show that zebrafish mcm5 mutation leads to DNA damage in immature T lymphocytes and the immature T cells sensitively undergo rapid cell death. Detailed analyses demonstrated that the immature T lymphocytes undergo rapid apoptosis via upregulation of tp53 and downregulation of bcl2 transcription in mcm5 mutants. Mechanistically, Mcm5 directly binds to Stat1a and facilitates its phosphorylation to enhance bcl2a expression under the conditions of DNA replication stress. However, in mcm5 mutants, the absence of the Mcm5-Stat1 complex decreases Stat1 phosphorylation and subsequent bcl2a transcription, accelerating apoptosis of immature T lymphocytes with genomic instability. Furthermore, our study shows that the role of Mcm5 in T-cell development is conserved in mice. In conclusion, our work identifies a role of Mcm5 in regulating T cell development via Stat1-Bcl2 cascade besides its role in DNA replication, providing a kind of mechanism by which immature T cells with gene mutation-induced DNA damage are rapidly cleared during T lymphocyte development.
研究领域:
免疫学
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遗传学
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肿瘤学
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分子生物学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Kaiping Sun,Xiaoyi Yan,Luo Li,Yunxue Zhu,Puyu Zhao,Zhiquan Zhang
Mercury pollution has always been a concern due to its high toxicity and persistence. In this study, we designed an aptamer-based sensor relying on lanthanide metal-organic framework and in-situ generation of active substances through electrochemical conversion. Firstly, a lanthanide metal-organic framework with rod-shaped structure was successfully synthesized, which has higher conductivity and stability compared to traditional MOFs. By possessing the substantial specific surface area and profusion of active sites offered by rod-shaped Gd 4 -MOF, gold nanoparticles (AuNPs) were deposited for the connection of tetrahedron DNA nanostructure (TDN). The active substances generated by electrochemical conversion has a more stringent arrangement and better response current compared to ordinary active substances, which can be used for ultra trace detection of mercury with higher sensitivity. After mercury added, DNA and mercury specifically bind to form thymine–Hg–thymine (T-Hg-T) structures, which cause changes in response current through conformational changes. The results demonstrated that a pronounced linear correlation between the DPV response and mercury concentration was observable, with a linear range of 1 fM to 200 fM and a detection limit of 0.66 fM. This sensor is successfully applied to detect the mercury content in marine products, so it has great application prospects for preventing and controlling mercury pollution.
研究领域:
环境科学
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分析化学
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纳米技术
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生物化学
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材料科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yang Li,Wanping Dang,Weirui Tang,Xingyu Chen,Jingjing Zhang
In this study, a novel imprinted electrochemical sensor was constructed by modifying deoxyribonucleic acid (DNA) for the detection of Ag + with high sensitivity and selectivity. Chitosan (CS) and Ag + template ions were mixed and used to modify a glassy carbon electrode, forming a composite CS-Ag + film. After CS-Ag + film crosslinking, gold nanoparticles (AuNPs) electrodeposition, and removal of Ag + templates, the imprinted sensor was prepared. A novel DNA-imprinted sensor was prepared by immobilizing Ag + DNA aptamers on the surface of AuNPs. The electroactive area, surface morphology and electrochemical reaction mechanism of the DNA-imprinted sensor were analyzed. The basic steps of the reaction were studied through electrochemical reaction kinetics analysis. The experimental results show that the DNA-imprinted electrochemical biosensor can quantitatively detect Ag + in the range of 10–50 nM (R 2 = 0.99695) with a limit of detection (LOD) of 8.25 nM (3σ/slope). The sensitivity of the electrochemical biosensor was 1.7473 × 10 −6 A/μM, and the effective area was 8.2121 cm 2 . The proposed novel DNA-imprinted sensor for detecting Ag + has high sensitivity and selectivity.
研究领域:
分析化学
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材料科学
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纳米技术
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生物传感器
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产品关联
DOI
高影响因子
Nature Protocols
·
2025
·
IF 16
Lv Zhaoyue,Li Peiran,Liu Mingxing,Yao Chi,Yang Dayong
Artificial DNA nanostructures, with their sequence programmability, precise molecular recognition and tunable stimuli responsiveness, bridge material chemistry and biomedicine. Here we detail the design and construction of hybridization chain reaction (HCR)-based DNA nanoframeworks, a class of DNA nanostructures with programmable sequences and customizable functions. HCR is an efficient, enzyme-free amplification strategy that isothermally produces nicked double-stranded DNA with periodically repeated modules via the assembly of two DNA hairpins, triggered by a DNA initiator. In contrast to other available assembly methods for the synthesis of DNA nanostructures, such as tile-mediated assembly, DNA origami and rolling circle amplification, the HCR method offers improved stability and efficiency under mild conditions, without reliance on enzymatic activity. The procedure uses radical polymerization to integrate DNA initiator into nanoframeworks, with overhangs complementary to functional sequences — termed linkers —which are amplified and incorporated through HCR. The linkers enable the incorporation of functional nucleic acid sequences. The HCR-based DNA nanoframeworks facilitate the loading capability of the delivered molecules, showing notable therapeutic efficacy and biosensing sensitivity. Preparation time for HCR-based DNA nanoframeworks ranges from 30 h to 45 h, depending on the payload.
研究领域:
生物化学
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纳米医学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2014
·Vol. 2
·期 12
·1667-1672
·
IF 0
Yan-Zhi Tian,Yan-Li Li,Zhi-Fei Wang,Yong Jiang
In this paper, a DNA–PEI microcapsule with novel nuclease-responsive controlled release properties was demonstrated. This microcapsule was constructed by the self-assembly of native DNA and PEI, and can work as a dual carrier for both DNA in the capsule shell and drugs inside the capsule. The controlled release behavior of the model drug BSA–FITC was studied using fluorescence microscopy and UV spectroscopy, and the results show that the synergetic controlled release can be triggered very well by a bio-stimulus, DNA nuclease recognition capsule decomposition, as well as the pH value and salt concentration. The therapeutic effects of the drug are enhanced due to the bio-stimuli responsive properties and the synergetic release of DNA and the drug together.
研究领域:
生物医学工程
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药物递送系统
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分子生物学
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材料科学
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转化医学
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DOI
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Haimeng Pei,Yiqun Zheng,Rongmei Kong,Lian Xia,Fengli Qu
We describe a highly sensitive nanoparticle–based fluorescence resonance energy transfer (FRET) probe developed without using molecular fluorophores as donors and acceptors. The success of this work relies on the strategy that DNA scission was designed to occur to the probe when target presented, which enabled the fluorescence signal “turn-on” of graphene quantum dots (GQDs) and thus quantitative analysis . In particular, amino-modified SiO 2 NPs were initially coated by GQDs to form highly emitting SiO 2 /GQDs, followed by conjunction with DNA functionalized gold nanoparticles (Au NPs–DNA) to form SiO 2 /GQDs–DNA–Au NPs composite. Owing to the FRET interactions between the GQDs and Au NPs, the fluorescence of GQDs was effectively quenched by Au NPs. When bleomycin (BLM), a model analyte , was mixed with the probe, the fluorescence signal of GQDs would be restored due to the removal of Au NPs from the SiO 2 /GQDs surface by DNA scission treatment with BLM in the presence of Fe (II). The current FRET probe shows a good linear relationship between the fluorescence intensity and the concentration of BLM in the range from 0.5 nM to 1 μM with a detection limit of 0.2 nM. The probe also shows satisfactory results for the analysis of clinical serum samples. This method provides versatility to the application of GQDs in FRET biosensing and could be potentially extended to other similar systems by replacing the linker between the GQDs and Au NPs.
研究领域:
生物化学
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纳米医学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2023
·Vol. 13
·期 9
·875
·
IF 5.4
Ziang Wu,Roujuan Kou,Kun Ni,Rui Song,Yuxuan Li,Tianhu Li,Hao Zhang
DNA ligases are essential enzymes involved in DNA replication and repair processes in all organisms. These enzymes seal DNA breaks by catalyzing the formation of phosphodiester bonds between juxtaposed 5′ phosphate and 3′ hydroxyl termini in double-stranded DNA. In addition to their critical roles in maintaining genomic integrity, DNA ligases have been recently identified as diagnostic biomarkers for several types of cancers and recognized as potential drug targets for the treatment of various diseases. Although DNA ligases are significant in basic research and medical applications, developing strategies for efficiently detecting and precisely quantifying these crucial enzymes is still challenging. Here, we report our design and fabrication of a highly sensitive and specific biosensor in which a stable DNA hairpin is utilized to stimulate the generation of fluorescence signals. This probe is verified to be stable under a wide range of experimental conditions and exhibits promising performance in detecting DNA ligases. We anticipate that this hairpin-based biosensor will significantly benefit the development of new targeting strategies and diagnostic tools for certain diseases.Keywords:DNA ligases;extraordinarily stable hairpins;biomarker detection;fluorescence probes;molecular sensing
研究领域:
分子生物学
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医学诊断
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2024
·
IF 4
Xue-Wen Liu,Liang Ma,Geng-Jun He,Xia Hu,Yuan-Dao Chen,Song-Bai Zhang
Photodynamic therapy (PDT) has been proven to be an important and effective therapy method with good spatial and temporal accuracy in clinical cancer treatment. Among many PDT candidates, Ru(II)-based compounds have received more attention due to their excellent photochemical and photophysical properties, high singlet oxygen ( 1 O 2 ) photogeneration, and DNA affinities. In this study, three ruthenium(II) complexes (RC1, RC2, RC3) have been designed by varying the aromatic plane area of the main ligand to adjust their 1 O 2 photogeneration, DNA affinities, DNA photodamage, and photocytoxicity. All complexes display no cytotoxicity in the dark against Hela, A549, HepG2, and BEL-7402 cells. RC1 displays no photocytotoxicity against all tumor cells. RC2 and RC3 showed high photocytotoxicity against BEL-7402 cells. The cellular uptake, cell migration, colonies assay, apoptosis analysis, and cell cycle analysis of RC2 and RC3 confirmed that the ruthenium(II) complexes might effectively enter the cancer cells, and lead to BEL-7402 cells apoptosis through DNA damage pathway. DNA photocleavage, DNA binding, and 1 O 2 measurement experiments demonstrated the DNA damage caused by RC2 and RC3 under light, compared to RC1, might be due to their high DNA affinities, large 1 O 2 quantum yields, and large lipophilicity by varying the aromatic plane area of the main ligand. Time dependent density functional theory (TDDFT) calculated results further show that two complexes exhibit the photocytotoxicity possibly via type II photoreaction mechanism because of the larger Δ S-T than O 2 and strong SOC between S n and T m . Hence, rational design of ruthenium(II) complexes by increasing DNA affinities, 1 O 2 quantum yields and lipophilicity can be regarded as an effective approach for enhanced photodynamic therapy (PDT).
研究领域:
生物化学
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肿瘤学
·
药理学
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DOI
高影响因子
INORGANICA CHIMICA ACTA
·
2025
·
IF 3.2
Peishan Huang,Wenhui Deng,Qiong Wu,Wanwan Hong,Jing Liu,Jiangbo Liang,Bin Zhao,Wenjie Mei
Herein, four ruthenium(II) complexes, [Ru(L) 2 (PEIP)(CH 2 ) 4 Br)](ClO 4 ) 2 (L = phen, 1 ; bpy, 2 , PEIP = 2-(2-phenyl-ethenyl)-1 H -imidazole-[4,5 f ][1,10]phenanthroline) and [Ru(L) 2 (PEIP)(CH 2 ) 4 Erianin)](ClO 4 ) 2 (L = phen, 1E ; bpy, 2E ) have been synthesized and investigated as promising inducer to promote conformation inversion of AS1411 G4 DNA. All of these complexes can bind and stabilize AS1411 G4 DNA. Subsequent investigations reveal that in the presence of 1 and 1E, the described circular dichroism signal of AS1411 G4 DNA was reversed. Furthermore, it is discovered that in the presence of 1 and 1E, AS1411 G4 DNA can condense to a somewhat loose nano-network. Moreover, the analysis using molecular docking demonstrates that the conformation inversion of AS1411 G4 DNA is largely dependent on the binding sites of 1 and 1E. In conclusion, these complexes will be used for tumor diagnostics in the clinical context because of their capacity to stably bind AS1411 G4 DNA and dramatically boost its fluorescence emission. Besides, these complexes show promise as inducers of AS1411 G4 DNA conformational inversion.
研究领域:
生物化学
·
药物化学
·
核酸化学
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产品关联
DOI
高影响因子
SENSORS
·
2018
·Vol. 18
·期 8
·2679
·
IF 2.475
Zheng-Jun Xie,Xian-Yu Bao,Chi-Fang Peng
A new colorimetric detection of methylmercury (CH3Hg+) was developed, which was based on the surface deposition of Hg enhancing the catalytic activity of gold nanoparticles (AuNPs). The AuNPs were functionalized with a specific DNA strand (HT7) recognizing CH3Hg+, which was used to capture and separate CH3Hg+by centrifugation. It was found that the CH3Hg+reduction resulted in the deposition of Hg onto the surface of AuNPs. As a result, the catalytic activity of the AuNPs toward the chromogenic reaction of 3,3,5,5-tetramethylbenzidine (TMB)-H2O2was remarkably enhanced. Under optimal conditions, a limit of detection of 5.0 nM was obtained for CH3Hg+with a linear range of 10–200 nM. We demonstrated that the colorimetric method was fairly simple with a low cost and can be conveniently applied to CH3Hg+detection in environmental samples.Keywords:methylmercury;gold nanoparticles;enzyme mimic;chromogenic reaction
研究领域:
环境科学
·
纳米生物学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）