ENVIRONMENTAL POLLUTION
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2023
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IF 8.9
Wenjing Zhang,Yue Han,Shuxin Li,Shengyu Wu,Juanfen Chai,Jincai Ma,Dayi Zhang,Yuesuo Yang
The diversity of colloidal types and the differences in the composite ratios in porous media are important factors governing the migration and biological risk of pathogenic microorganisms in the subsurface environment. In this study, E. coli O157:H7 was subjected to co-migration experiments with different compositions of the composite colloid montmorillonite (MMT)-Fe 2 O 3 , and the biomolecular response of E. coli under the action of colloids was analyzed by Raman spectroscopy to quantify the risk of E. coli under the action of composite colloids based on both. The results showed that Fe 2 O 3 colloids inhibited E. coli migration mainly by electrostatic adsorption and reduced E. coli metabolism. MMT colloid inhibited E. coli migration mainly by blockage, and E. coli metabolism increased, and surface macromolecules decreased to reduce E. coli adhesion. MMT-Fe 2 O 3 complex colloids inhibited migration through electrostatic attraction between the two and formation of cohesive colloids, with reduced E. coli metabolism and insignificant biomolecular response. It was briefly assessed that the composite colloids reduced E. coli risk less strongly than single colloids, stemming from the difference in the mechanism of influence and the actual need to consider colloid interactions. This conclusion can inform the management and control of pathogen risk in porous media environments.
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CHINESE JOURNAL OF CHEMICAL ENGINEERING
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2023
·
IF 3.8
Xiaoyun Hou,Qinghong Shi
We described a novel polymer-lipase conjugate for high-efficient esterification of vitamin E using vitamin E and succinic anhydride as the substrates in nonaqueous media. In this work, the monomer, N -isopropylacrylamide (NIPAM), was grafted onto Candida rugosa lipase (CRL) to synthesize poly(NIPAM) ( p NIPAM)-CRL conjugate by atom transfer radical polymerization via the initiator coupled on the surface of CRL. The result showed that the catalytic efficiencies of p NIPAM-CRL conjugates (19.5 – 30.3 L·s -1 ·mmol -1 ) were at least 7-times higher than that of free CRL (2.36 L·s -1 ·mmol -1 ) in DMSO. It was attributed to a significant increase in K cat of the conjugates in nonaqueous media. The synthesis catalyzed by p NIPAM-CRL conjugates was influenced by the length and density of the grafted polymer, water content, solvent polarity and molar ratio of the substrates. In the optimal synthesis, the reaction time was shortened at least 7-times, and yields of vitamin E succinate by p NIPAM- g -CRL and free CRL were obtained to be 75.4% and 10.1 % at 55°C after the reaction for 1.5 h. The result argued that conjugation with p NIPAM induced conformational change of the lid on CRL based on hydrophobic interaction, thus providing a higher possibility of catalysis-favorable conformation on CRL in nonaqueous media. Moreover, p NIPAM conjugation improved the thermal stability of CRL greatly, and the stability improved further with an increase of chain length of p NIPAM. At the optimal reaction conditions (55°C and 1.5 h), p NIPAM- g -CRL also exhibited good reusability in the enzymatic synthesis of vitamin E succinate and kept ∼ 70% of its catalytic activity after ten consecutive cycles. The research demonstrated that p NIPAM- g -CRL was a more competitive biocatalyst in the enzymatic synthesis of vitamin E succinate and exhibited good application potential under harsh industrial conditions.
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ACS Sustainable Chemistry & Engineering
·
2023
·
IF 8.4
Xiaoxia Liu,Yangyang Wang,Ronghao Liu,Ping Lv,Wenfeng Xue,Jinxin Guo,Huiying Wei,Yanzhao Yang
With environmental problems and a shortage of resources, it is urgent to recover gold from electronic waste (e-waste). Meanwhile, it is necessary to explore the relationships between the structures and extraction performances of ionic liquids (ILs) to further improve the extraction capacities of ILs for gold. Herein, three pyrrolidinium-based ILs with various N-substituents were synthesized for solvent-free extraction of Au(III). The three pyrrolidinium-based ILs exhibited ultrahigh extraction capacities for Au(III) with the order of [Pyr-EA][NTf2] (524.6 mg·g–1) > [Pyr-Bu][NTf2] (457.3 mg·g–1) > [Pyr-C2OC2][NTf2] (403.5 mg·g–1). The effects of N-substituents on extraction performances were quantitatively investigated by electrostatic potential (ESP) and quantitative NMR. Among them, the ester group (−COOR) is the most beneficial to the extraction process, which is associated with an increased ESP value and boosted hydrophobicity. Notably, [Pyr-EA][NTf2] displayed outstanding selectivity in multimetal solutions; especially, it could selectively and efficiently extract Au(III) from the actual CPU. Furthermore, [Pyr-EA][NTf2] possesses significant reuseability and can maintain superior extraction efficiency of 84.3% after five cycles. These excellent performances together with low cost ($0.44·g–1 [Pyr-EA][NTf2]) and considerable profit ($29.72·g–1 [Pyr-EA][NTf2]) confirmed that [Pyr-EA][NTf2] is a promising prospect for sustainable recovery of gold from e-waste.
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BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Ying Gan,Ning Hu,Chuanjiang He,Shuqi Zhou,Jiawei Tu,Tao Liang,Yuxiang Pan,Dmitry Kirsanov,Andrey Legin,Hao Wan,Ping Wang
Urolithiasis commonly occurs in kidney and ureteral, and may cause local organ/tissue damage, even kidney failure. The incidence of this disease is increasing worldwide, in which calcium oxalate is the major composition forming the urinary calculus. Therefore, to monitor this disease for the prevention and treatment, measuring the oxalate in the urine is of great significance. Here, a rapid and sensitive colorimetric method was developed based on 3,3',5,5'-tetramethylbenzidine-manganese dioxide (TMB-MnO 2 ) nanosheets for oxalate detection. MnO 2 nanosheets acted as an efficient biomimetic oxidase to catalyze the reaction with TMB and oxalate. Pale yellow TMB can be oxidized to blue oxide TMB catalyzed by BSA-stabilized MnO 2 nanosheets, and oxalate can selectively inhibit this reaction by consuming and reacting with MnO 2 nanosheets, thus achieving the quantitative detection of oxalate. Moreover, a home-made bionic electronic-eye (E-eye) system was developed as a portable in-situ detection platform to efficiently measure the oxalate concentrations in 10 s by direct photographing. By optimizing experimental conditions, this method shows a wide linear range (7.8 μM to 250 μM) and a low detection limit (0.91 μM) for oxalate detection. Besides, this method exhibits high selectivity even with 80-fold interfering chemicals. Furthermore, the performance of the method was validated by testing the artificial urine samples, indicating its great potential for monitoring and diagnosis of urolithiasis in point-of-care applications.
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FOOD CHEMISTRY
·
2018
·
IF 4.946
Chun Wang,Keyu Xing,Ganggang Zhang,Meifang Yuan,Shaolan Xu,Daofeng Liu,Wenyao Chen,Juan Peng,Song Hu,Wei-Hua Lai
Escherichia coli O157:H7 ( E. coli O157:H7) is a potential threat to human health; thus, a rapid and sensitive method for detecting it is necessary. We designed a single-stranded DNA that contained an appended block and anchoring block. The appended block acted as a scaffold to prepare fluorescent Ag nanoclusters (AgNCs). The anchoring block contained Poly A, which bound with the surface of gold nanoparticles to quench the fluorescence of AgNCs. An interesting ELISA approach for detecting E. coli O157:H7 was established via fluorescent quenching of DNA-stabilized AgNCs by using a sandwich complex. The changes in fluorescence intensity of AgNCs were used to quantitatively detect E. coli O157:H7. The sensitivity for detecting E. coli O157:H7 reached 1.905 × 10 3 CFU/mL with a good linear range. Compared with conventional ELISA, the sensitivity of this technique increased by 30-fold. Moreover, this method demonstrated specificity and reproducibility and could be used in food samples.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2018
·
IF 3.997
De-Sheng Liang,Jian Liu,Tao-Xing Peng,Hui Peng,Feng Guo,Hai-Jun Zhong
Cancer stem cells (CSCs) can resist conventional chemotherapy to lead to cancer recurrence. For complete eradication of cancers, an effective CSCs therapeutic strategy should be developed to combine with conventional chemotherapy. In this work, a novel vitamin E-based redox-sensitive salinomycin (SAL, an inhibitor for CSCs) prodrug nanoparticles (TS NPs) and hyaluronic acid (HA)-coated TS NPs (HTS NPs) were fabricated to deliver paclitaxel (PTX) for cancer-targeted and combined chemotherapy. Both TS and HTS prodrug NPs had mean diameter of about 200 nm with uniform size distribution, excellent drug loading capacity for PTX, and glutathione-triggered SAL and PTX release profiles. The HTS prodrug NPs had enhanced cellular uptake efficiency over TS NPs due to CD44 receptor-mediated endocytosis, hence exerting stronger potency of SAL upon CSCs-enriched mammospheres formation and G 0 /G 1 cell phase arresting. Cytotoxicity and 3D tumor spheroids assays demonstrated that both TS and HTS prodrug NPs themself can synergize with loaded PTX to maximize the chemotherapeutic effect. Obviously, the latter demonstrated a more potent anticancer efficacy due to improved intracellular drug delivery efficiency. These results suggested that the designed TS prodrug NPs, especially the coated HTS NPs can serve as an effective anti-CSCs strategy for cancer targeted and combination treatments.
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JOURNAL OF BIOMATERIALS APPLICATIONS
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2018
·
IF 2.082
Yude Liu,Lian Rao,Hongguan Zhang,Yanyou Cen,Kaili Cheng
Herb borneol is usually used in clinics for the treatment of central nervous system illness, for its ability of blood–brain barrier permeability, although its poor water solubility and poor bioavailability limit its clinical application to some degree. In this study, we developed a novel nanoparticle combining the benefits of vitamin E d-ɑ-tocopheryl poly(ethylene glycol) succinate (E-TPGS) (or TPGS) and guar gum to get TPGS-g-guar gum as a drug delivery system to carry borneol, which could improve the solubility of borneol and increase the drug-loading capacity efficiently. The results showed that TPGS-g-guar gum nanoparticles delivery system was suitable to carry borneol and release the drug effectively, and TPGS-g-guar gum/borneol nanoparticles would be a potential platform for improving the treatment of central nervous system illness and cerebrovascular disease.
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Small
·
2024
·
IF 13
Baozhong Chen,Kangxin Shen,Yaping Li,Bo Huang,Huiming Su,Jintao Xu,Shuai Yang,Qi Zhou,Linfeng Lan,Junbiao Peng,Yong Cao
Sensing pressure and temperature are two important functions of human skin that integrate different types of tactile receptors. In this paper, a deformable artificial flexible multi-stimulus-responsive sensor is demonstrated that can distinguish mechanical pressure from temperature by measuring the impedance and the electrical phase at the same frequency without signal interference. The electrical phase, which is used for measuring the temperature, is totally independent of the pressure by controlling the surface micro-shapes and the ion content of the ionic film. By doping the counter-ion exchange reagent into the ionic liquid before pouring, the upper temperature measuring limit increases from 35 to 50 °C, which is higher than the human body temperature and the ambient temperature on Earth. The sensor shows high sensitivity to pressure (up to 0.495 kPa −1 ) and a wide temperature sensing range (−10 to 50 °C). A multimodal ion-electronic skin (IE M -skin) with an 8 × 8 multi-stimulus-responsive sensor array is fabricated and can successfully sense the distribution of temperature and pressure at the same time. Finally, the sensors are used for monitoring the touching motions of a robot-arm finger controlled by a remote interactive glove and successfully detect the touching states and the temperature changes of different objects.
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Food Chemistry-X
·
2024
·
IF 6.5
Shan Jiang,Pengfei Jiang,Dingding Feng,Meiran Jin,Hang Qi
The flavor of algae was one of the key factors for consumer acceptance. The objective of this study was to investigate the characteristic volatile compounds in cooking and seasoned cooking edible brown seaweeds ( Undaria pinnatifida and Laminaria japonica ). The gas chromatography-ion mobility spectrometry (GC-IMS) and electronic nose (E-nose) analysis showed that baking resulted in significant difference in flavor of brown seaweeds. However, the overall effect of cooking was not as significant as that of the seasoning solution treatment. Additionally, brown seaweeds treated with the seasoning solution were more acceptable. Undaria pinnatifida was found to contain 72 volatile flavor compounds, while Laminaria japonica had a total of 70. This study proved the applicability of GC-IMS combined with E-nose technology to detect the changes of volatile components of brown seaweeds after processing, providing beneficial knowledge and basic theory for the deep processing of brown seaweeds.
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Nano Energy
·
2025
·
IF 17.1
Shengxin Xiang,Xiao Wei,Lei Liu,Jianlong Hong,Shengshun Duan,Huiyun Zhang,Jinqiu Huang,Zhishui Chen,Zhiwei Zhao,Qiongfeng Shi,Jun Wu
Electrodes are transitioning from flexible to stretchable modality to accommodate more complex application scenarios. Here, inspired by the human skin, we report a rapidly formed Janus-structured stretchable (JSS) electrode. Wherein, the phase separation technology enables rapid film formation, and the gravity effect constructs the Janus structure. The Janus structure aggregates liquid metal (LM) on one side and thermoplastic polyurethane (TPU) on the other, providing a combination of highly stretchable and highly conductive nature at the same time. The JSS film has fast film forming time (15 s), metal-like conductivity (10 6 S/m), high stretchability (260 %), permeable (4077.41 g m −2 day −1 ), ultra-thin thickness (50–1000 μm), strain-insensitivity (stretching, folding, and rotation states), as well as excellent cuttable, transferable, recyclable, reconfigurable, and biocompatible property. Furthermore, the JSS films successfully achieve the effective acquisition of physiological, triboelectric, and resistive signals, when applied as standalone devices and integrated electrodes for electrophysiological signal recording, energy harvesting, and wearable sensing. With its prominent performance and broad adaptability, the proposed JSS film is expected to significantly advance the field of stretchable electrodes and further promote the practical applications of flexible and stretchable devices in industrial and electronic skin.
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Food & Function
·
2017
·Vol. 8
·期 7
·2601-2610
·
IF 3.247
Meihui Fan,Guowen Zhang,Junhui Pan,Deming Gong
Dihydromyricetin (DMY), a natural flavonoid, was found to effectively inhibit tyrosinase activity in a mixed-type manner with an IC50 value of (3.66 ± 0.14) × 10−5 mol L−1. DMY combined with the dietary vitamin D3 at lower concentrations exhibited a synergistic effect on the inhibition of tyrosinase. The formation of a DMY–tyrosinase complex led to fluorescence quenching and conformational changes of tyrosinase, which was driven mainly by hydrophobic interactions and hydrogen bonds. The molecular simulation further found that DMY inserted into the active pocket of tyrosinase interacted with amino acid residues Tyr78, His85, and Ala323, occupying the catalytic center of tyrosinase to hinder entrance of the substrate, leading to the inhibition of tyrosinase. This study may provide a scientific foundation for screening effective tyrosinase inhibitors.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2016
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IF 3.994
Jing Wang,Peng Xue,Jiahua Zhou,Lin Li,Lu Xu,Yongjun Wang
To achieve optimal therapeutic index of docetaxel , we conjugated docetaxel (DTX) with vitamin E (VE) by ester bond with disulfide bond or thioether bond as spacer to produce DTX-ss-VE or DTX-s-VE. The two prodrugs were successfully loaded into liposomes . The physicochemical characterization and in vitro release profiles of the prodrug loaded liposomes were investigated. MTT assays showed that the cytotoxicity of DTX-ss-VE loaded liposomes on PC3 (IC 50 = 27.5 nM) and A549 cells (IC 50 = 63.4 nM) was comparable with the cytotoxicity of DTX-s-VE loaded liposomes (IC 50 = 99.2 and 159.5 nM, respectively). The pharmacokinetic studies demonstrated that DTX-ss-VE and DTX-s-VE loaded liposomes exhibited an extended DTX half-life (3.6 ± 1.2 and 10.0 ± 3.0 h, respectively) as compared to DTX solutions (2.1 ± 1.5 h) and an increased AUC (30487.3 ± 3791.6 and 20922.1 ± 5633.3 ng/L × h, respectively) compared with DTX solutions (1779.3 ± 226.6 ng/L × h). Finally, the in vivo anti-tumor studies showed that DTX-ss-VE loaded liposomes possessed similar antitumor activity compared with DTX solutions. Unexpectedly, not any tumor inhibition effect was observed in DTX-s-VE loaded liposomes group. In a conclusion, our studies suggested that simplex favorable properties of the anticancer prodrug can not ensure available good therapeutic index and the rational design of prodrug needs a comprehensive understanding of the in vitro and in vivo behavior of the prodrug.
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