INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
·
IF 8.7
Yueling Shang,Jinhui Yi,Ying Fan,Shihaoran Xu,Xin Zhao,Xujia Dai,Xia Zhao,Yanli Liu,Jinbin Liu,Xiaohong Yu
In this study, sea buckthorn juice phenolics, free, bound, and conjugated fractions, were investigated for pancreatic lipase (PL) inhibition, and conjugated phenolics (CPs) were found to exhibit the highest inhibitory activity (IC50: 253.9 μg/mL pre-fermentation, 185.2 μg/mL post-fermentation). Fermentation modified the conjugated phenolic composition, upregulating 31 compounds and downregulating 18 compounds, notably protocatechuic acid and isorhamnetin derivatives. Importantly, kinetic analysis demonstrated a fermentation-induced shift in the inhibition mode from mixed-type (unfermented CPs [CCP]) to uncompetitive (fermented CPs [FCP]), suggesting its potential application as an adjunctive therapy. Spectroscopic studies indicated that CP-PL interactions occurred through static quenching and hydrophobic forces, with FCP inducing secondary structural perturbations in PL. Notably, FCP demonstrated synergistic effects with orlistat, suggesting its potential for combined therapeutic applications. These results highlight the value of sea buckthorn as a source of anti-obesity polyphenols and provide a mechanistic basis for the development of functional ingredients that target PL activity regulation. These findings suggest that fermented sea buckthorn phenolics are promising candidates for metabolic disorder interventions owing to their dual inhibition mechanism and structural modulation of PL.
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高影响因子
Food and Bioprocess Technology
·
2026
·Vol. 19
·期 9
·440
·
IF 5.6
Huang Huisha,Chen Shizhuo,Xu Xinyi,Mao Xin,Shou Yiwen,Lu Qinpei,Feng Chao,Ye Yu,Guo Hongwei,Su Zhiheng,Huang Zhaoquan
Sea cucumber peptide products are valued for their high cost and potent biological activity, making it essential to monitor Escherichia coli contamination to ensure quality control. A novel sandwich assay had been developed, integrating magnetic enrichment with positive magnetic beads, magnetophoretic chromatographic separation, and nanozyme-mediated colorimetric photothermal detection to quantify Escherichia coli O157:H7 ( E. coli O157:H7). This approach used amino-functionalized magnetic nanoparticles (Fe 3 O 4 @SiO 2 -NH 2 ) for effective bacterial concentration, demonstrating substantial capture efficiency. Magnetophoretic separation further enhanced enrichment, achieving a capture efficiency exceeding 85% for high-concentration samples (10 7 CFU/mL). An aptamer specific to E. coli O157:H7, modified on V-doped manganese dioxide nanozyme encapsulated by ZIF-90 (V-MnO 2 @ZIF-90), served as the secondary binding agent, ensuring high specificity. This configuration resulted in the formation of the Fe 3 O 4 @SiO 2 -NH 2 - E. coli O157:H7-V-MnO 2 @ZIF-90/Apt sandwich complex. Upon the addition of ABTS, both colorimetric and photothermal signals were generated, yielding a detection limit of 1 × 10 CFU/mL for sea cucumber peptides samples, with the total detection time being approximately 75 min. This method presents significant promise for the rapid separation and detection of pathogenic bacteria in food products.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Jinghe Sun,Weiyun Zheng,Xiaoyan Wang,Yimeng Li,Jingfeng Yang
The freshness in A. japonicus after thymol treatment was tested. The results showed that sensory scores in thymol group significantly increased 14.83 and 21.67 compared to the Frozen and BK groups after storage. Texture parameters showed that hardness, springiness, and chewiness of body wall treated with thymol were 2.56, 1.21, and 2.02 folds that of the BK group, respectively. Volatile flavor results revealed 38 flavor substances and 33 volatile organic compounds in the body wall. Aldehydes, alcohols, heterocycles, and alkane were the key sources to odor components of A. japonicus , including nonanal, hexanal, 1-octanol, 1-hexanol, d -limonene, and 2-methylbutyl acetate. Moreover, heptenoic acid, dimethyl disulfide, and dimethylamine, as distinct volatile flavor substances, were the main contributors of fishy, malodorous, and spicy smells of autolysis A. japonicus . This study guides the potential use of thymol in sea cucumber preservation and seeks the spoiled flavor markers for non-destructive rapid testing technology development.
研究领域:
食品科学
·
海洋生物学
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Biology-Basel
·
2024
·Vol. 13
·期 9
·
IF 3.6
Yong Liu,Jiahua Cheng
The spatial niche has garnered significant attention in ecological research, particularly regarding species distribution patterns. The East China Sea, known for its favorable natural conditions and abundant fishery resources, exhibits diverse spatial distribution patterns among species, shaped by their seasonal physiological needs. This study utilized a habitat suitability index model to explore the spatial distribution patterns of key fishery resources in the East China Sea across four seasons and their interactions. Two methodologies were employed to identify key environmental factors and assess the ecological niche overlap among different species and seasons. Results indicated that the initial method identified water temperature as the critical factor for hairtail, while the subsequent method emphasized water temperature and salinity for hairtail, salinity for small yellow croaker, and water depth for Bombay duck. The main spatial habitat overlap was observed between paired species, likely driven by predator-prey interactions. During summer and autumn, increased overlap among multiple species was primarily influenced by synchronized life cycles. An overlap index formula quantified the seasonal species overlap, showing an increase from spring to winter, reflecting changes in convergent habitat preferences. The peak overlap occurred in winter, driven by overwintering, reduced food competition, and enhanced coexistence potential, while the lowest overlap was noted in spring as overwintering ended and predation and competition intensified.
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MOLECULES
·
2019
·Vol. 24
·期 18
·3292
·
IF 3.06
Shiyuan Fang,Qianqian Zhou,Yan Hu,Feng Liu,Jun Mei,Jing Xie
The objective of this research was to explore the antimicrobial activity and mechanism of carvacrol againstVibrio Parahemolyticus,Shewanella putrefaciens,Staphylococcus aureusandPseudomonas fluorescensand evaluate the effect of the addition of carvacrol/β-cyclodextrin emulsions to flaxseed gum (FSG)-sodium alginate (SA) edible films on the preservation of Chinese sea bass (Lateolabrax maculatus) fillets during refrigerated storage. The minimum inhibitory concentration (MIC) of carvacrol againstV. parahemolyticus,S. putrefaciens,S. aureusandP. fluorescenswere 0.5, 0.5, 0.125, and 0.5 mg/mL, respectively. Alkaline phosphatase activity assay, nucleotide and protein leakage, and scanning electron microscope demonstrated that carvacrol damaged the external structure of the tested bacterial cells causing leakage of cytoplasmic components. At the same time, when FSG-SA films containing carvacrol used as coating agents for Chinese sea bass fillets cold storage, FSG-SA films containing 1.0 or 2.0 mg/mL carvacrol could significantly reduce TVB-N content, K-value, the degree of microbial deterioration and maintain quality of sea bass fillets according to organoleptic evaluation results.
研究领域:
食品科学与技术
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食品微生物学
·
活性包装
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水产品保鲜
·
天然抗菌剂
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
·
IF 6
Yamei Wu,Huan Xiang,Shengjun Chen,Yongqiang Zhao,Qiuxing Cai,Wanling Lin,Yanyan Wu,Yueqi Wang
Ionic strength affects the aggregation behavior and function of myofibrillar proteins (MPs). This study investigated changes in the aggregation behavior and function of sea bass MPs under ion induction to determine the mechanism of its impact. The ionic strength increased the solubility of proteins (up to 90% at 0.6 mol/L), facilitated the transition from α-helices to β-sheets and increased the reactive sulfhydryl groups from 3.12 μmol/g to 3.93 μmol/g. The unfolding of MPs ultimately changed their functional properties. Scanning electron microscopy images showed that a gel network structure began to form at an ionic strength of 0.4 mol/L. Furthermore, the water holding capacity and cooking yield of the gel increased from 51.40% to 28.97%–69.01% and 46.16%, respectively, with an increasing ionic strength. The distribution of droplets in the field of view was relatively uniform at an ionic strength of 0.6 mol/L with a minimum value of CI (20.66%). However, the droplets aggregated at higher ionic strengths (>0.8 mol/L), which also decreased the emulsification properties and gel properties. It is hoped that these results will broaden the potential applications of myofibrillar proteins in emulsions and provide guidance for the processing of surimi products.
研究领域:
食品科学
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蛋白质化学
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水产养殖
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SCIENCE OF THE TOTAL ENVIRONMENT
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2021
·
IF 7.963
Yang Liu,JinAo You,YuJun Li,JiaoDi Zhang,Yong He,Florian Breider,Shu Tao,WenXin Liu
Studies focused only on surface water may underestimate microplastic abundance in aquatic environments. This was the first survey to investigate the vertical (surface, intermediate, and bottom waters) distribution and composition of microplastics (MPs) in the water columns and surface sediments collected from an urban seagoing river in northern China. Microplastic abundance in the water columns ranged from 5.6 to 31.4 items∙L −1 and from 2141 to 10,035 items∙kg −1 dry weight (dw) in the surface sediments. Polyethylene dominated throughout the water columns to the surface sediments, in which low- and high-density polyethylene (LDPE and HDPE) were dominant in the water columns and surface sediments, respectively. The dominant shape of MPs was fibers/lines in both the water column and the surface sediment samples. Different from the estimations, the average abundance of MPs in the surface and intermediate waters was significantly lower ( p < 0.01) than that in the bottom water, which may be due to the resuspension of small-sized MPs in the bottom water. As the MPs size increased, their vertical distributions in the water columns were more affected by the water depth. The results showed that MPs were detained in the water columns of river system, and the high concentrations of MPs in the bottom water could not be neglected.
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高影响因子
BIOLOGICAL TRACE ELEMENT RESEARCH
·
2018
·Vol. 186
·期 2
·554-561
·
IF 2.361
Liu Hao,Luo Jiaoyang,Ding Tong,Gu Shanyong,Yang Shihai,Yang Meihua
In this paper, a simple and cost-effective method using high-performance liquid chromatography in conjunction with inductively coupled plasma mass spectrometry with a rapid ultrasound-assisted extraction was used for analysis speciation of trace mercury in sea cucumber species of Apostichopus japonicus . The effective separation of inorganic mercury, methylmercury, and ethylmercury was achieved within 10 min using Agilent ZORBAX SB-C 18 analytical and guard columns with an isocratic mobile phase consisting of 8% methanol and 92% H 2 O containing 0.12% L-cysteine ( m / v ) and 0.01 mol/L ammonium acetate. Mercury species were extracted from A. japonicus samples using a solution containing 2-mercaptoethanol, L-cysteine, and hydrochloric acid and sonicating for 0.5 h. The limits of detection of inorganic mercury, methylmercury, and ethylmercury were 0.12, 0.08, and 0.20 μg/L, and the minimum detectable concentrations (measured at 0.500 g sample volume in 10.00 mL) were 2.4, 1.6, and 4.0 μg/kg, respectively. Analysis of a scallop certified reference material (GBW 10024) revealed accordance between the experimental and certified values. This study provides a reference for the evaluation of mercury speciation in sea cucumber and other seafood.
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CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Huadong Fu,Yan Qin,Zhengwei Peng,Jipeng Dou,Zhixiong Huang
Lightweight phenolic matrix composites are highly potential in ablative thermal protective materials for future aerospace vehicles, and hybrid modification is considered as one of effective ways to improve the oxidation and ablation resistance of phenolic matrix. Herein, the co-continuous silica zirconium hybrid phenolic resin (SZRx) was synthesized, which can be used to prepare lightweight ablation resistant aerogel composites by the sol-gel method. The SZRx aerogel showed stable porous structure and excellent hydrophobicity, and the silicon zirconium hybrid greatly improved the high temperature carbon residue and oxidation resistance of phenolic resin. The SZRx aerogel composites also possessed excellent integrated characteristics of heat protection and insulation, with a linear ablation rate as low as 0.032 mm/s and a back temperature below 200 °C. The synergistic effect of silicon and zirconium formed the multiphase ceramic layer (C, SiO 2 , SiC and ZrO 2 ) with the sea-island-like structure on the surface of the composite material, which can effectively resist high-temperature ablation and aerodynamic exfoliation. This can indicate that silicon zirconium dual element hybrid phenolic resin is the highly competitive matrix for lightweight and ablation resistant composite materials.
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JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Min Du,Haokun Shi,Rui Yin,Jianlei Yang,Fulin Shi,Qinggong Zheng,Yu Zhou,Ruixue Guo,Wanqing Wu
Oil spills are a global environmental protection challenge, and traditional adsorption materials have poor effect on low temperature and high viscosity marine oil spills. This article reports composite adsorption materials TDA/rGO@WS for viscous oil spills: loaded with rGO/TDA coating on a commercial biomass wood pulp sponge (WS), achieving Joule heating , photothermal effect and hydrophobic modification. The results showed that the TDA/rGO@WS has good photothermal conversion ability and Joule heating ability, and the temperature increased to 83.7 °C and 148 °C, respectively, under simulated solar irradiation and additional voltage at room temperature. The efficiency of adsorption at a low temperature of 5 °C increased by 22.41% at 1 sun and by 51.53% at 10 V. Temperature effectively reduced the viscosity of the offshore oil spill and ensures the efficient adsorption of oil spills at low temperatures promoted. The TDA/rGO@WS also showed good hydrophobicity (WCA=129°), excellent efficiency of water–oil separation (99.53%) and good adsorption capacity (9.4–13.68 g/g) for marine fuel oils. TDA/rGO@WS effectively solves the problem of cleaning up high–viscosity oil spills from ships in winter and polar waters, and proposes a new strategy for all–weather efficient treatment of oil spills at sea.
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高影响因子
AQUACULTURE
·
2025
·
IF 3.9
Song Wang,Liu Song,Yaru Li,Zhenxin Zhao,Ting Xue,Guoxiang Luan,Huarong Guo
Holothurian cell culture remains unsuccessful due to the lacking of suitable medium and tissues. Here, a reliable in vitro cell culture system has been developed for Apostichopus japonicus by optimizing the basal and growth media as well as the sourced tissues. Blood vessel and longitudinal muscle tissues were first found to be the best two sourced tissues. Subsequently, the composition and content of free amino acids and carbohydrates in holothurian coelomic fluid were analyzed and the obtained data were used to design and obtain an optimal basal medium (SCBM-4). Next, an optimal growth medium (SCGM-24) was further developed by supplementing 1 % FBS, 25 % coelomic fluid and 20 % body wall enzymatic hydrolysate of sea cucumbers into SCBM-4. Primary blood vessel (SCBV) cells could be efficiently isolated by mechanical dissociation in tissue dissociation apparatus and survive healthily for 22 d in SCBM-4, and 58 d in SCGM-24 with a gradually decreased proliferation activity. Primary longitudinal muscle (SCLM) cells could only be efficiently isolated by explant culture and healthily survive for 52 d in SCBM-4 but with no proliferation activity, whereas their viabilities decreased sharply in SCGM-24. The epithelial-shaped SCBV cells could be successfully sub-cultured for 3 times using 4 °C PE solution, whereas the loosely attached round-shaped SCBV and SCLM cells could be passaged for 6 and 4 times by pipetting, respectively. Additionally, shrimp covert mortality nodavirus (CMNV) could cross-species infect and multiply in SCBV cells, and acute heat shock treatment could significantly upregulate the transcriptional expression of heat shock protein genes.
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高影响因子
Food Packaging and Shelf Life
·
2025
·
IF 10.6
Yanqing Li,Xuchen Li,Shaomei Li,Jiao Li,Xiang Zou,Hongwei Zhan,Guanyuan Qu,Lixiu Zhang,Yongjun Sun,Wenming Ju,Mengqi Ye,Pengdong Sun,Jingyuan Li,Yang Deng,Ting Ding
Aeromonas sobria , recognized as a psychrotrophic specific spoilage organism in refrigerated aquatic products, facilitates its proliferation by metabolizing proteins, lipids, and carbohydrates, thereby generating spoilage-associated metabolites and compromising product quality. Quorum sensing (QS), a bacterial cell-to-cell communication mechanism, orchestrates the secretion of virulence and spoilage factors in A. sobria via the LuxI/R QS system. Targeting this regulatory pathway presents a novel strategy to mitigate spoilage and extend the shelf life of aquatic products. Previous studies have demonstrated that zingerone (ZRN) exhibits quorum-quenching activity at sub-inhibitory concentrations; however, its practical application is constrained by limited solubility and bioavailability. To overcome these challenges, ZRN was encapsulated within halloysite nanotubes (HNTs) and incorporated into a self-assembled composite film composed of chitosan (CS) and sodium carboxymethyl cellulose (CMC-Na). The fabricated film was characterized for its structural, thermal, and mechanical properties and subsequently evaluated for its preservation efficacy on refrigerated perch fillets. The composite film exhibited a homogeneous surface morphology with uniformly dispersed HNTs, alongside adequate thermal stability, tensile strength, and physicochemical attributes suitable for refrigerated aquatic product preservation. Notably, the film suppressed spoilage indicators, including total volatile basic nitrogen (TVB-N) accumulation, lipid peroxidation, and bacterial spoilage potential, without inhibiting A. sobria growth. Furthermore, it mitigated muscle tissue degradation and biogenic amine formation in perch fillets during refrigerated storage. Molecular docking simulations revealed that ZRN competitively binds to the LuxR-type receptor at the same binding pocket as the endogenous autoinducer C 4 -HSL, thereby disrupting QS signaling. Collectively, this ZRN-loaded composite film represents a promising biodegradable packaging solution for enhancing the quality and shelf life of refrigerated aquatic foods.
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危险品化学品经营许可证（带存储）